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The map label for this gene is yibK [C]

Identifier: 37520268

GI number: 37520268

Start: 745636

End: 746124

Strand: Reverse

Name: yibK [C]

Synonym: gll0699

Alternate gene names: 37520268

Gene position: 746124-745636 (Counterclockwise)

Preceding gene: 37520269

Following gene: 37520265

Centisome position: 16.01

GC content: 61.55

Gene sequence:

>489_bases
ATGCCCCCAGCAGCAATGGGCCGTGAAGCTTTCCATGTCGTGCTGGTGGATCCGGAGATCCCTCCCAACACCGGCAATGT
GGCGCGCACCTGTGCCGCCACCGGCACCAGCCTGCATCTGGTAGGGACTTTGGGCTTTCAACTGAGCGACAAATACCTCA
AGCGTGCCGGGCTCGACTACTGGGAACACGTCGATTTGCATTACCATCCGGATCTGGGCGCGTTGCTGGAACAGCAATCC
GCGCAGCGCTTCGTGCTCACCAGTGCCCGCCGGGGAGTGTGCCTGTGGGACTTTGCTTTTCAGCCCGGCGACTGGCTGGT
CTTTGGCTCCGAGAGCCGCGGCCTGCCCGAGGGTCTACTGTCAGACGAACGCCATCCGGTGGTGACCATTCCCCTCGCCG
AACCGGCGGTACGCAGTTTGAATCTCTCCAACGCCGTGGCTGTGGTCCTTTTTGAATCGCTTCGCCAGTTGGACCCGCCT
TCAAGCTAA

Upstream 100 bases:

>100_bases
GAGTACGTGGGCCGCATCTACGAAGAGATCAAAAACCGGCCGCTCTATATCCTCAAGGACGCCCAGGGGTTCGAGCGCCC
GGTACCGGGGCGGGTGGGCA

Downstream 100 bases:

>100_bases
AGCCTATTCACCCTTGGCTGCAGAGAAAAGTATCTTTCCCTTTTTAGATCCAACCCACCGCCAGTGCCAGGGTTCGTAGC
CGACACCCACGGCGTTGCCT

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MPPAAMGREAFHVVLVDPEIPPNTGNVARTCAATGTSLHLVGTLGFQLSDKYLKRAGLDYWEHVDLHYHPDLGALLEQQS
AQRFVLTSARRGVCLWDFAFQPGDWLVFGSESRGLPEGLLSDERHPVVTIPLAEPAVRSLNLSNAVAVVLFESLRQLDPP
SS

Sequences:

>Translated_162_residues
MPPAAMGREAFHVVLVDPEIPPNTGNVARTCAATGTSLHLVGTLGFQLSDKYLKRAGLDYWEHVDLHYHPDLGALLEQQS
AQRFVLTSARRGVCLWDFAFQPGDWLVFGSESRGLPEGLLSDERHPVVTIPLAEPAVRSLNLSNAVAVVLFESLRQLDPP
SS
>Mature_161_residues
PPAAMGREAFHVVLVDPEIPPNTGNVARTCAATGTSLHLVGTLGFQLSDKYLKRAGLDYWEHVDLHYHPDLGALLEQQSA
QRFVLTSARRGVCLWDFAFQPGDWLVFGSESRGLPEGLLSDERHPVVTIPLAEPAVRSLNLSNAVAVVLFESLRQLDPPS
S

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=153, Percent_Identity=43.7908496732026, Blast_Score=126, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17711; Mature: 17580

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPAAMGREAFHVVLVDPEIPPNTGNVARTCAATGTSLHLVGTLGFQLSDKYLKRAGLDY
CCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCEEEEEEECCCEECHHHHHHCCCCC
WEHVDLHYHPDLGALLEQQSAQRFVLTSARRGVCLWDFAFQPGDWLVFGSESRGLPEGLL
HHCCCEEECCCHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCCEEEECCCCCCCCCHHC
SDERHPVVTIPLAEPAVRSLNLSNAVAVVLFESLRQLDPPSS
CCCCCCEEEEECCCCHHHHCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PPAAMGREAFHVVLVDPEIPPNTGNVARTCAATGTSLHLVGTLGFQLSDKYLKRAGLDY
CCCCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCEEEEEEECCCEECHHHHHHCCCCC
WEHVDLHYHPDLGALLEQQSAQRFVLTSARRGVCLWDFAFQPGDWLVFGSESRGLPEGLL
HHCCCEEECCCHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCCEEEECCCCCCCCCHHC
SDERHPVVTIPLAEPAVRSLNLSNAVAVVLFESLRQLDPPSS
CCCCCCEEEEECCCCHHHHCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]