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The map label for this gene is glnB [H]

Identifier: 37520258

GI number: 37520258

Start: 736711

End: 737079

Strand: Direct

Name: glnB [H]

Synonym: gvip070

Alternate gene names: 37520258

Gene position: 736711-737079 (Clockwise)

Preceding gene: 37520257

Following gene: 37520259

Centisome position: 15.81

GC content: 62.87

Gene sequence:

>369_bases
ATGAACAAAATCGAAGCGATCATCCAACCTTCCAGGCTCGACGCCGTCGAGCAGGCCCTGGCGGCCATCGGTGTGCAGGG
CATGACCGTCAGCCGGGTTGAAGGCTTCGGCCGGCAGCGCGGCCATTCGGTGATCTTTCGGGGATTCGGGAGCGCCGCGC
AGTTTCTTGCCAAGCTCAAACTCGAAATCGTCGTACCTGACGAATTCACCGAGACGGTTGTCGAGCGAATCGTACAGGCG
GCACGCACCGGCAGCGTCGGCGACGGCAAAATCTTTGTGCTGCCTGTCACCCAGGCGGTGCGGGTGCGCACCGGCGAGTG
CGACCTGGAGGCGGTCCGCTTCGAGAGTCCGGCAGTCCTGATTCACTGA

Upstream 100 bases:

>100_bases
TGAATTGAGCGGCTGCTGAATAGAGGGATTCCCGAGTTTTTCACCGGCAAGGTCTAGCCTGAATGCACCTGCAGCCGCTC
ATCCTGAGGAACGCACCGCC

Downstream 100 bases:

>100_bases
GGGCACCATGTTCACAAAAATCCTCGTCGCCCTAGATCAGTCTTCCAGCAACGAACTGGTCTTCGAGAAAGCCTTTGCCC
TCGCCCGCCTCGGCGGAGGG

Product: nitrogen regulatory protein P-II

Products: NA

Alternate protein names: PII signal transducing protein [H]

Number of amino acids: Translated: 122; Mature: 122

Protein sequence:

>122_residues
MNKIEAIIQPSRLDAVEQALAAIGVQGMTVSRVEGFGRQRGHSVIFRGFGSAAQFLAKLKLEIVVPDEFTETVVERIVQA
ARTGSVGDGKIFVLPVTQAVRVRTGECDLEAVRFESPAVLIH

Sequences:

>Translated_122_residues
MNKIEAIIQPSRLDAVEQALAAIGVQGMTVSRVEGFGRQRGHSVIFRGFGSAAQFLAKLKLEIVVPDEFTETVVERIVQA
ARTGSVGDGKIFVLPVTQAVRVRTGECDLEAVRFESPAVLIH
>Mature_122_residues
MNKIEAIIQPSRLDAVEQALAAIGVQGMTVSRVEGFGRQRGHSVIFRGFGSAAQFLAKLKLEIVVPDEFTETVVERIVQA
ARTGSVGDGKIFVLPVTQAVRVRTGECDLEAVRFESPAVLIH

Specific function: P-II indirectly controls the transcription of the glutamine synthetase gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of glnA. When P-II is uridylylated to P-II-UMP, these events are reversed

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=52.6785714285714, Blast_Score=129, Evalue=7e-32,
Organism=Escherichia coli, GI1786655, Length=108, Percent_Identity=43.5185185185185, Blast_Score=108, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918
- InterPro:   IPR002332 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 13203; Mature: 13203

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIEAIIQPSRLDAVEQALAAIGVQGMTVSRVEGFGRQRGHSVIFRGFGSAAQFLAKLK
CCCCHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEEEECCCHHHHHHHHHE
LEIVVPDEFTETVVERIVQAARTGSVGDGKIFVLPVTQAVRVRTGECDLEAVRFESPAVL
EEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECCCCCEEEEEECCCEEE
IH
EC
>Mature Secondary Structure
MNKIEAIIQPSRLDAVEQALAAIGVQGMTVSRVEGFGRQRGHSVIFRGFGSAAQFLAKLK
CCCCHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEEEECCCHHHHHHHHHE
LEIVVPDEFTETVVERIVQAARTGSVGDGKIFVLPVTQAVRVRTGECDLEAVRFESPAVL
EEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECCCCCEEEEEECCCEEE
IH
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7921264 [H]