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The map label for this gene is nth [H]

Identifier: 37520225

GI number: 37520225

Start: 706853

End: 707551

Strand: Reverse

Name: nth [H]

Synonym: gll0656

Alternate gene names: 37520225

Gene position: 707551-706853 (Counterclockwise)

Preceding gene: 37520227

Following gene: 37520223

Centisome position: 15.19

GC content: 67.67

Gene sequence:

>699_bases
ATGAGTGCAATCCGGCAAGAGCGCCGCGCCCGGGCCGAGCGTCTGCTGGTAAAGCTGAAGGTGGCCTATCCCCGGGGCCT
CACCCTGGGCCTCAGCAGCACCAACCCGTTCGAGTATCTGGTGGCGACGGTGCTTGCCACCCAGTGCAGAGACGAGCGGG
TCAACAAAATCACCCCGGCGCTGTTCGCCCGCTATCCCGACCCGGCCGCCTTCGCCGCCGCCGATTACGAGGCGCTGTTG
CCCCTGGTGCGCCCCACCGGTCTCGGTCCCACCAAGGCGCGCAACTTGACGGCCATCGGCCGGCTTTTGCTCGAACGCCA
CGCAGGCAAAGTGCCGGCCACGATGGCGGAACTGACCGCCCTGCCGGGGGTGGCCCGCAAGATCGCCAATCTGGTGCTTG
CCGACTGCCACGGCATCGTCGAGGGCGTGGCGGTCGATACCCATGTGCGGCGCATCTCAAAACTGTTGGGGCTGACCGAC
TCGACCGACGCCGCCAAAATCGAGCGCGATCTGATGGACTGCCTGCCGCGCGACGCCTGGCGCAGCTGGAACAACTTGAT
GGTCGAGCACGGCCGCCAGTGTTGTGTGGCGGGTGCTCCCCGCTGTACGGCCTGCCCGCTGGTCGAAGATTGCCCCGGCG
GCCGGGAACTGACGGCAGAACTGGAGAGGCGCGATGCGGCCGGTGCGGTTCTGCCTTGA

Upstream 100 bases:

>100_bases
GGGGCTTGAGCCTATCGTAGCTGGGCACACTGCACGCTATGGATAAAACCGGCCACCCAGGATGTGGCATCTTTGAGAAT
GTCCCTCCACCCGGCAACCG

Downstream 100 bases:

>100_bases
TCGCTAGGACTGCTCAGGCGGGTAAGGCATCACCTCGCGCCCGTCGGGCAGTTCGCTCAGGTGCTGCTCAAGGGAGCCGG
GCGAGCCGATGGGCGAACCG

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MSAIRQERRARAERLLVKLKVAYPRGLTLGLSSTNPFEYLVATVLATQCRDERVNKITPALFARYPDPAAFAAADYEALL
PLVRPTGLGPTKARNLTAIGRLLLERHAGKVPATMAELTALPGVARKIANLVLADCHGIVEGVAVDTHVRRISKLLGLTD
STDAAKIERDLMDCLPRDAWRSWNNLMVEHGRQCCVAGAPRCTACPLVEDCPGGRELTAELERRDAAGAVLP

Sequences:

>Translated_232_residues
MSAIRQERRARAERLLVKLKVAYPRGLTLGLSSTNPFEYLVATVLATQCRDERVNKITPALFARYPDPAAFAAADYEALL
PLVRPTGLGPTKARNLTAIGRLLLERHAGKVPATMAELTALPGVARKIANLVLADCHGIVEGVAVDTHVRRISKLLGLTD
STDAAKIERDLMDCLPRDAWRSWNNLMVEHGRQCCVAGAPRCTACPLVEDCPGGRELTAELERRDAAGAVLP
>Mature_231_residues
SAIRQERRARAERLLVKLKVAYPRGLTLGLSSTNPFEYLVATVLATQCRDERVNKITPALFARYPDPAAFAAADYEALLP
LVRPTGLGPTKARNLTAIGRLLLERHAGKVPATMAELTALPGVARKIANLVLADCHGIVEGVAVDTHVRRISKLLGLTDS
TDAAKIERDLMDCLPRDAWRSWNNLMVEHGRQCCVAGAPRCTACPLVEDCPGGRELTAELERRDAAGAVLP

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=183, Percent_Identity=33.879781420765, Blast_Score=104, Evalue=7e-23,
Organism=Escherichia coli, GI1787920, Length=203, Percent_Identity=34.4827586206897, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1789331, Length=183, Percent_Identity=27.3224043715847, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17554540, Length=201, Percent_Identity=31.8407960199005, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324530, Length=196, Percent_Identity=25.5102040816327, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319304, Length=169, Percent_Identity=28.9940828402367, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45550361, Length=196, Percent_Identity=28.0612244897959, Blast_Score=88, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 25169; Mature: 25037

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIRQERRARAERLLVKLKVAYPRGLTLGLSSTNPFEYLVATVLATQCRDERVNKITPA
CCHHHHHHHHHHHHHHHHHEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LFARYPDPAAFAAADYEALLPLVRPTGLGPTKARNLTAIGRLLLERHAGKVPATMAELTA
HHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LPGVARKIANLVLADCHGIVEGVAVDTHVRRISKLLGLTDSTDAAKIERDLMDCLPRDAW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHH
RSWNNLMVEHGRQCCVAGAPRCTACPLVEDCPGGRELTAELERRDAAGAVLP
HHHHHHHHHCCCCEEECCCCCCCCCCCHHCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SAIRQERRARAERLLVKLKVAYPRGLTLGLSSTNPFEYLVATVLATQCRDERVNKITPA
CHHHHHHHHHHHHHHHHHEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LFARYPDPAAFAAADYEALLPLVRPTGLGPTKARNLTAIGRLLLERHAGKVPATMAELTA
HHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LPGVARKIANLVLADCHGIVEGVAVDTHVRRISKLLGLTDSTDAAKIERDLMDCLPRDAW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHH
RSWNNLMVEHGRQCCVAGAPRCTACPLVEDCPGGRELTAELERRDAAGAVLP
HHHHHHHHHCCCCEEECCCCCCCCCCCHHCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]