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The map label for this gene is ndhI

Identifier: 37520223

GI number: 37520223

Start: 704296

End: 704925

Strand: Reverse

Name: ndhI

Synonym: gvip068

Alternate gene names: 37520223

Gene position: 704925-704296 (Counterclockwise)

Preceding gene: 37520225

Following gene: 37520222

Centisome position: 15.13

GC content: 60.32

Gene sequence:

>630_bases
ATGGTAAAGTTCCTGGAAAAAGTTGGTGGCTATGCCAGGGATGTGCTGGAATCGGCCAAGTATATCGGTCAGGGCATGGG
GGTCGTCTTCGACCACATGCGCCGAAAACCGGTCACCGTCCAGTACCCATACGAAAAGCTCATCCCGTCGGAGCGCTTTC
GCGGGCGCATCCACTTCGAGCGGCCCAAGTGCATTTCTTGCGAAGTGTGCGTGCGCGTCTGCCCGATCAACCTGCCCGTG
GTCGATTACGAGTTCAACAAAGAGACCAAGAAAAAAGAACTCAACTCCTACTCGATCGATTTTGGCGTCTGCATCTTTTG
CGGCAACTGTGTCGAGTACTGCCCCACCAGTTGCCTGTCGATGACCGAGGAGTACGAGCTTTCGGTCTACGACCGCCACG
AACTCAACTACGACGATGTCGCCCTCGGACGGCTGCCCACCCGGGTCACCGACGACCCGGTGGTGCGCCCCATTCGCGAA
CTGGCCTATCTGCCCAAGGGAGTCATGGACGGCCACCTCGAGGATCCCGGCAGCCGCCGCGCCGGGGAATTGCCCGAGGA
GATCGTCGCCCGGTTGCGCCCGGCCGACCACAGCAGCGGTACCGACGCCAACAAGAAGGAGGGCGAGTGA

Upstream 100 bases:

>100_bases
CGCTCCCGAGGCAGTCAGCCGCTCCCCCGCGCGCGGGCTTCTGGCCTGTGTAAACGAATGATCTTAAAATGAAGCGTGAT
CTCGAATGAGCATATTAGCA

Downstream 100 bases:

>100_bases
CATTTTCTGAAGGGGTCCAACTGGTTTCGTTTATCATCCTCACCGCCATCGTGCTCGGTGGGGCGGCGGGGGTGGTGCTC
CTGCGCAGCATGGTCCACTC

Product: NADH dehydrogenase subunit I

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I subunit I; NDH-1 subunit I; NDH-I

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MVKFLEKVGGYARDVLESAKYIGQGMGVVFDHMRRKPVTVQYPYEKLIPSERFRGRIHFERPKCISCEVCVRVCPINLPV
VDYEFNKETKKKELNSYSIDFGVCIFCGNCVEYCPTSCLSMTEEYELSVYDRHELNYDDVALGRLPTRVTDDPVVRPIRE
LAYLPKGVMDGHLEDPGSRRAGELPEEIVARLRPADHSSGTDANKKEGE

Sequences:

>Translated_209_residues
MVKFLEKVGGYARDVLESAKYIGQGMGVVFDHMRRKPVTVQYPYEKLIPSERFRGRIHFERPKCISCEVCVRVCPINLPV
VDYEFNKETKKKELNSYSIDFGVCIFCGNCVEYCPTSCLSMTEEYELSVYDRHELNYDDVALGRLPTRVTDDPVVRPIRE
LAYLPKGVMDGHLEDPGSRRAGELPEEIVARLRPADHSSGTDANKKEGE
>Mature_209_residues
MVKFLEKVGGYARDVLESAKYIGQGMGVVFDHMRRKPVTVQYPYEKLIPSERFRGRIHFERPKCISCEVCVRVCPINLPV
VDYEFNKETKKKELNSYSIDFGVCIFCGNCVEYCPTSCLSMTEEYELSVYDRHELNYDDVALGRLPTRVTDDPVVRPIRE
LAYLPKGVMDGHLEDPGSRRAGELPEEIVARLRPADHSSGTDANKKEGE

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI4505371, Length=136, Percent_Identity=30.8823529411765, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI1789075, Length=103, Percent_Identity=33.0097087378641, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788617, Length=114, Percent_Identity=33.3333333333333, Blast_Score=66, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17555194, Length=124, Percent_Identity=32.258064516129, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17864306, Length=137, Percent_Identity=30.6569343065693, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NDHI_GLOVI (Q7NMW1)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_923600.1
- ProteinModelPortal:   Q7NMW1
- SMR:   Q7NMW1
- GeneID:   2599479
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.654
- HOGENOM:   HBG724359
- OMA:   HYSIDFG
- ProtClustDB:   PRK05888
- BioCyc:   GVIO251221:GLL0654-MONOMER
- HAMAP:   MF_01351
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR004497
- InterPro:   IPR010226
- Gene3D:   G3DSA:1.10.1060.10
- TIGRFAMs:   TIGR00403
- TIGRFAMs:   TIGR01971

Pfam domain/function: NA

EC number: 1.6.5.3

Molecular weight: Translated: 23831; Mature: 23831

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKFLEKVGGYARDVLESAKYIGQGMGVVFDHMRRKPVTVQYPYEKLIPSERFRGRIHFE
CCHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEECCHHHCCCCHHHCCCEEEC
RPKCISCEVCVRVCPINLPVVDYEFNKETKKKELNSYSIDFGVCIFCGNCVEYCPTSCLS
CCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHHHCCCCCCHHHEEHHCCHHHHCCHHHHH
MTEEYELSVYDRHELNYDDVALGRLPTRVTDDPVVRPIRELAYLPKGVMDGHLEDPGSRR
CCHHHEEEEEECCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
AGELPEEIVARLRPADHSSGTDANKKEGE
CCCCHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVKFLEKVGGYARDVLESAKYIGQGMGVVFDHMRRKPVTVQYPYEKLIPSERFRGRIHFE
CCHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEECCHHHCCCCHHHCCCEEEC
RPKCISCEVCVRVCPINLPVVDYEFNKETKKKELNSYSIDFGVCIFCGNCVEYCPTSCLS
CCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHHHCCCCCCHHHEEHHCCHHHHCCHHHHH
MTEEYELSVYDRHELNYDDVALGRLPTRVTDDPVVRPIRELAYLPKGVMDGHLEDPGSRR
CCHHHEEEEEECCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
AGELPEEIVARLRPADHSSGTDANKKEGE
CCCCHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 14621292