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The map label for this gene is bioF

Identifier: 34499835

GI number: 34499835

Start: 4723962

End: 4725113

Strand: Reverse

Name: bioF

Synonym: CV_4380

Alternate gene names: 34499835

Gene position: 4725113-4723962 (Counterclockwise)

Preceding gene: 34499836

Following gene: 34499834

Centisome position: 99.45

GC content: 71.09

Gene sequence:

>1152_bases
ATGCGTCTACAAGATCTGTCCCCCGGCCTCGGGGAGTTGGACGAGCGCCACCGCCTGCGCAGGCGCGCGACGCTGGAGTC
GCCGCAAGGCGATGAGGTCGTCATCGACGGCCGCTCCTATATTTCGTTCGCCAGCAACGACTATCTCGGCCTGGCCGACC
ACCCGTCGCTGGTGCGCGCGCTGCAGCAAGGCGCCGACCGCTGGGGCGCCGGCAGCGGCGCCTCCCATCTGCTGACCGGC
CATACCCTCGCCCACCAGCAGGCGGAAGAGGCGCTGGCCGGCTTCGTCGGCCGCGAGGCCGCGCTGCTGTTCGGTTCCGG
CTACGCCGCCAACCTGGCGGTGATCACCAGCCTGGTCGGTCGCGGCGACGCGGTGTTCGCCGACAAGCTCAACCACGCTT
CGCTGAACGACGGCTGTCTGCTGTCGCGGGCCGATTTCCAGCGCTTCCGCCACAACGACCTCGCCCATCTCGAGCAATTG
CTGGCGGACAGCGGCGCGCCGACCAAGCTGATCGCGGTGGACGCCGTGTACAGCATGGACGGCGACCAGGCGCCGCTGCC
GGCGCTGCTGGCGCTGGCGGAGCGCTACGACGCCTGGCTGTACGTCGACGACGCCCACGGCTTCGGCGTGCTGGGCGACG
GCCGCGGCTCGGCCGCCCTGCACGGGCTCGCCAGCGATCGGCTGATCTACATGGCGACGCTGGGCAAGGCCGCCGGCTTG
GCCGGCGCCTTCGTCGCCGGCTGCCGGCCCTTGGTCGACTGGCTGGTGAACAAGGCGCGCACCTATATCTACACCACCGC
CCAGCCGCCGGCGCTGGCGGCCGCGGTGGGCGCCAGCCTGCGGCTGATCGCCGAGGGCGGCGAACGGCGCGAGCGCCTGC
GGCAACTGATCTCGCGCTGCCGGCAGCGGCTGGAGGGCACGCCTTACGCCGGCGCGTCGGCCACGGCCATCCAGCCTTTC
GTCGTCGGTTCGGACGAGAACGCGGTCCGATTGGCGGAGACGCTGCGGGAACAGGGCTACTGGGTGCCGGCGGTCAGGCC
GCCTACCGTCCCGGAAAACGGCGCGCGGCTGCGGATCTCGCTGTCGGCCCGGCATGAGCTTTCCCAATTGGACGCATTAC
TGGATCTGATGCTGCGGCCAGCGGAAAGCTGA

Upstream 100 bases:

>100_bases
AGTAATTCTGCGCTGTCAGCTTAAATGCAAACATATATATAGTATGTCGTGCCGGGGCGGGCTTGCCGCGGTTCGGCTCC
TAACCCAACCAGGTACAAGC

Downstream 100 bases:

>100_bases
CGCGCGCGGCGGCGGACCGGCTCCGCAACCAAGCGAAAACGAGCAGGCCCGGGGCGTTTGCCCCGGGCTTTTGTCTGACT
GCGGACTTTCTCGGCGTCCC

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MRLQDLSPGLGELDERHRLRRRATLESPQGDEVVIDGRSYISFASNDYLGLADHPSLVRALQQGADRWGAGSGASHLLTG
HTLAHQQAEEALAGFVGREAALLFGSGYAANLAVITSLVGRGDAVFADKLNHASLNDGCLLSRADFQRFRHNDLAHLEQL
LADSGAPTKLIAVDAVYSMDGDQAPLPALLALAERYDAWLYVDDAHGFGVLGDGRGSAALHGLASDRLIYMATLGKAAGL
AGAFVAGCRPLVDWLVNKARTYIYTTAQPPALAAAVGASLRLIAEGGERRERLRQLISRCRQRLEGTPYAGASATAIQPF
VVGSDENAVRLAETLREQGYWVPAVRPPTVPENGARLRISLSARHELSQLDALLDLMLRPAES

Sequences:

>Translated_383_residues
MRLQDLSPGLGELDERHRLRRRATLESPQGDEVVIDGRSYISFASNDYLGLADHPSLVRALQQGADRWGAGSGASHLLTG
HTLAHQQAEEALAGFVGREAALLFGSGYAANLAVITSLVGRGDAVFADKLNHASLNDGCLLSRADFQRFRHNDLAHLEQL
LADSGAPTKLIAVDAVYSMDGDQAPLPALLALAERYDAWLYVDDAHGFGVLGDGRGSAALHGLASDRLIYMATLGKAAGL
AGAFVAGCRPLVDWLVNKARTYIYTTAQPPALAAAVGASLRLIAEGGERRERLRQLISRCRQRLEGTPYAGASATAIQPF
VVGSDENAVRLAETLREQGYWVPAVRPPTVPENGARLRISLSARHELSQLDALLDLMLRPAES
>Mature_383_residues
MRLQDLSPGLGELDERHRLRRRATLESPQGDEVVIDGRSYISFASNDYLGLADHPSLVRALQQGADRWGAGSGASHLLTG
HTLAHQQAEEALAGFVGREAALLFGSGYAANLAVITSLVGRGDAVFADKLNHASLNDGCLLSRADFQRFRHNDLAHLEQL
LADSGAPTKLIAVDAVYSMDGDQAPLPALLALAERYDAWLYVDDAHGFGVLGDGRGSAALHGLASDRLIYMATLGKAAGL
AGAFVAGCRPLVDWLVNKARTYIYTTAQPPALAAAVGASLRLIAEGGERRERLRQLISRCRQRLEGTPYAGASATAIQPF
VVGSDENAVRLAETLREQGYWVPAVRPPTVPENGARLRISLSARHELSQLDALLDLMLRPAES

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily

Homologues:

Organism=Homo sapiens, GI7657118, Length=358, Percent_Identity=34.3575418994413, Blast_Score=205, Evalue=6e-53,
Organism=Homo sapiens, GI284448556, Length=339, Percent_Identity=34.5132743362832, Blast_Score=199, Evalue=5e-51,
Organism=Homo sapiens, GI119220554, Length=359, Percent_Identity=32.3119777158774, Blast_Score=175, Evalue=7e-44,
Organism=Homo sapiens, GI83977442, Length=345, Percent_Identity=33.3333333333333, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI83977440, Length=345, Percent_Identity=33.3333333333333, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI83977444, Length=356, Percent_Identity=32.5842696629214, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI4502025, Length=372, Percent_Identity=31.7204301075269, Blast_Score=168, Evalue=9e-42,
Organism=Homo sapiens, GI40316939, Length=372, Percent_Identity=31.7204301075269, Blast_Score=168, Evalue=9e-42,
Organism=Homo sapiens, GI4758668, Length=358, Percent_Identity=31.8435754189944, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI5454084, Length=331, Percent_Identity=28.7009063444109, Blast_Score=131, Evalue=9e-31,
Organism=Escherichia coli, GI1786993, Length=352, Percent_Identity=46.0227272727273, Blast_Score=290, Evalue=2e-79,
Organism=Escherichia coli, GI1790046, Length=364, Percent_Identity=35.7142857142857, Blast_Score=216, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI71994529, Length=365, Percent_Identity=32.0547945205479, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI32566772, Length=357, Percent_Identity=32.2128851540616, Blast_Score=158, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17560912, Length=360, Percent_Identity=30.8333333333333, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17560914, Length=360, Percent_Identity=30.8333333333333, Blast_Score=148, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI71982625, Length=287, Percent_Identity=27.5261324041812, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71982617, Length=287, Percent_Identity=27.5261324041812, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320438, Length=363, Percent_Identity=31.404958677686, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6320267, Length=356, Percent_Identity=28.6516853932584, Blast_Score=140, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6323954, Length=297, Percent_Identity=25.2525252525253, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24662918, Length=364, Percent_Identity=33.2417582417582, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI17137420, Length=342, Percent_Identity=33.6257309941521, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI17136286, Length=357, Percent_Identity=29.6918767507003, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24653280, Length=309, Percent_Identity=24.9190938511327, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24653276, Length=309, Percent_Identity=24.9190938511327, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24653278, Length=309, Percent_Identity=24.9190938511327, Blast_Score=115, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOF_CHRVO (Q7NPW2)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_904050.1
- HSSP:   P12998
- ProteinModelPortal:   Q7NPW2
- GeneID:   2549579
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_4380
- NMPDR:   fig|243365.1.peg.4380
- HOGENOM:   HBG649839
- OMA:   KDYASND
- PhylomeDB:   Q7NPW2
- BioCyc:   CVIO243365:CV_4380-MONOMER
- HAMAP:   MF_01693
- InterPro:   IPR022834
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR00858

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.47

Molecular weight: Translated: 41027; Mature: 41027

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 20-20 BINDING 132-132 BINDING 178-178 BINDING 349-349

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLQDLSPGLGELDERHRLRRRATLESPQGDEVVIDGRSYISFASNDYLGLADHPSLVRA
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEECCCCCCCCCCCHHHHHH
LQQGADRWGAGSGASHLLTGHTLAHQQAEEALAGFVGREAALLFGSGYAANLAVITSLVG
HHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHC
RGDAVFADKLNHASLNDGCLLSRADFQRFRHNDLAHLEQLLADSGAPTKLIAVDAVYSMD
CCCEEEHHCCCCCCCCCCEEEEHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEEEEEECC
GDQAPLPALLALAERYDAWLYVDDAHGFGVLGDGRGSAALHGLASDRLIYMATLGKAAGL
CCCCCHHHHHHHHHHCCEEEEEECCCCCEEEECCCCCHHHHCCCCCCEEEEEECCHHHHH
AGAFVAGCRPLVDWLVNKARTYIYTTAQPPALAAAVGASLRLIAEGGERRERLRQLISRC
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCCCEEEEECCCHHHHHHHHHHHHH
RQRLEGTPYAGASATAIQPFVVGSDENAVRLAETLREQGYWVPAVRPPTVPENGARLRIS
HHHHCCCCCCCCCCCEECEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEE
LSARHELSQLDALLDLMLRPAES
EHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRLQDLSPGLGELDERHRLRRRATLESPQGDEVVIDGRSYISFASNDYLGLADHPSLVRA
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEECCCCCCCCCCCHHHHHH
LQQGADRWGAGSGASHLLTGHTLAHQQAEEALAGFVGREAALLFGSGYAANLAVITSLVG
HHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHC
RGDAVFADKLNHASLNDGCLLSRADFQRFRHNDLAHLEQLLADSGAPTKLIAVDAVYSMD
CCCEEEHHCCCCCCCCCCEEEEHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEEEEEECC
GDQAPLPALLALAERYDAWLYVDDAHGFGVLGDGRGSAALHGLASDRLIYMATLGKAAGL
CCCCCHHHHHHHHHHCCEEEEEECCCCCEEEECCCCCHHHHCCCCCCEEEEEECCHHHHH
AGAFVAGCRPLVDWLVNKARTYIYTTAQPPALAAAVGASLRLIAEGGERRERLRQLISRC
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCCCEEEEECCCHHHHHHHHHHHHH
RQRLEGTPYAGASATAIQPFVVGSDENAVRLAETLREQGYWVPAVRPPTVPENGARLRIS
HHHHCCCCCCCCCCCEECEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEE
LSARHELSQLDALLDLMLRPAES
EHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782