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The map label for this gene is bioB

Identifier: 34499836

GI number: 34499836

Start: 4725222

End: 4726205

Strand: Reverse

Name: bioB

Synonym: CV_4381

Alternate gene names: 34499836

Gene position: 4726205-4725222 (Counterclockwise)

Preceding gene: 34499840

Following gene: 34499835

Centisome position: 99.48

GC content: 66.26

Gene sequence:

>984_bases
ATGCATTCTGCCACGATGGTGTTCAAGCGCGCGGCCACCCCGCACCCGGAAAAAGCCGAATGGAGCGTGGACGACGTGGA
GGCTTTGCTGGGGCTGCCCTTCATGGAGCTGGTGTTTCGCGCCGCCGAAATCCACCGCCAGTTCTTCGACCCGACCAAGG
TCCAGCTGTCGACGCTGGTGTCGATCAAGACCGGCGGCTGTCCGGAAGACTGCGGCTACTGCCCGCAGTCCGTTCATCAC
GACACGCCGGTGGCCGACCAGCCGATGATGACGGTGGACGAGGTGGTGGCGGCGGCGCGCCAGGCCAAGGCCAACGGCGC
CGGCCGCTTCTGCATGGGCGCGGCCTGGCGCGGACCCAAGGACGCCGATCTGCAGAAGACGCTGGACATGGTGCGCGAAG
TGAAGGCGCTGGGCCTGGAGACCTGCGCCACTTTCGGCCTGCTGCGCGACGGCCAGGCCGAGCAGTTGAAGGACGCCGGC
CTCGACTATTACAACCATAACCTGGACACCGCGCCGGACAAGTACGGCGACATCATCCAGACCCGGGAGTACGAGGACCG
GCTGGACACGCTGGGCAAGGTGCGCAAGGCCGGGCTGTCGGTATGCTGCGGGGGCATCGTCGGCATGAATGAAACCCGCC
GCGACCGCGCCGGTCTGATCGTGCAGCTGGCCAATCTGGATCCGCAGCCGGAGTCGGTGCCGGTCAACAACCTGGTGCAG
GTGGTCGGCACGCCGCTCGAGGGCGCCGAGCGCCTGGACTGGACGGAATTCGTGCGCACCATCGCGGTGGCGCGGATCAC
GATGCCGGCCAGCTACGTCAGGCTGTCGGCGGGCCGCCGCGAAATGGACGAAGCCACCCAGGCGCTGTGTTTCCTGGCCG
GCGCCAACTCCATCTTCTACGGCGACAAGCTGTTGACGACCGGCAATCCGGACGTGGTGGCCGACCAATCGCTGATGGCT
AAGCTGAATTTACAGCCGTTGTAA

Upstream 100 bases:

>100_bases
CATGTCGCCAGCCCTCCAAGATTTTGTCGCCTGCCCATCGGAGCAGGATGACGAGCCCATGGTTCGCATTGAAAATGACA
AACAGTTATTGGAGCATACG

Downstream 100 bases:

>100_bases
GAAACGTTAGTAATTCTGCGCTGTCAGCTTAAATGCAAACATATATATAGTATGTCGTGCCGGGGCGGGCTTGCCGCGGT
TCGGCTCCTAACCCAACCAG

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MHSATMVFKRAATPHPEKAEWSVDDVEALLGLPFMELVFRAAEIHRQFFDPTKVQLSTLVSIKTGGCPEDCGYCPQSVHH
DTPVADQPMMTVDEVVAAARQAKANGAGRFCMGAAWRGPKDADLQKTLDMVREVKALGLETCATFGLLRDGQAEQLKDAG
LDYYNHNLDTAPDKYGDIIQTREYEDRLDTLGKVRKAGLSVCCGGIVGMNETRRDRAGLIVQLANLDPQPESVPVNNLVQ
VVGTPLEGAERLDWTEFVRTIAVARITMPASYVRLSAGRREMDEATQALCFLAGANSIFYGDKLLTTGNPDVVADQSLMA
KLNLQPL

Sequences:

>Translated_327_residues
MHSATMVFKRAATPHPEKAEWSVDDVEALLGLPFMELVFRAAEIHRQFFDPTKVQLSTLVSIKTGGCPEDCGYCPQSVHH
DTPVADQPMMTVDEVVAAARQAKANGAGRFCMGAAWRGPKDADLQKTLDMVREVKALGLETCATFGLLRDGQAEQLKDAG
LDYYNHNLDTAPDKYGDIIQTREYEDRLDTLGKVRKAGLSVCCGGIVGMNETRRDRAGLIVQLANLDPQPESVPVNNLVQ
VVGTPLEGAERLDWTEFVRTIAVARITMPASYVRLSAGRREMDEATQALCFLAGANSIFYGDKLLTTGNPDVVADQSLMA
KLNLQPL
>Mature_327_residues
MHSATMVFKRAATPHPEKAEWSVDDVEALLGLPFMELVFRAAEIHRQFFDPTKVQLSTLVSIKTGGCPEDCGYCPQSVHH
DTPVADQPMMTVDEVVAAARQAKANGAGRFCMGAAWRGPKDADLQKTLDMVREVKALGLETCATFGLLRDGQAEQLKDAG
LDYYNHNLDTAPDKYGDIIQTREYEDRLDTLGKVRKAGLSVCCGGIVGMNETRRDRAGLIVQLANLDPQPESVPVNNLVQ
VVGTPLEGAERLDWTEFVRTIAVARITMPASYVRLSAGRREMDEATQALCFLAGANSIFYGDKLLTTGNPDVVADQSLMA
KLNLQPL

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=309, Percent_Identity=58.252427184466, Blast_Score=382, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6321725, Length=316, Percent_Identity=46.5189873417722, Blast_Score=310, Evalue=3e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_CHRVO (Q7NPW1)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_904051.1
- HSSP:   P12996
- ProteinModelPortal:   Q7NPW1
- SMR:   Q7NPW1
- GeneID:   2549803
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_4381
- NMPDR:   fig|243365.1.peg.4381
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- PhylomeDB:   Q7NPW1
- BioCyc:   CVIO243365:CV_4381-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 35740; Mature: 35740

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSATMVFKRAATPHPEKAEWSVDDVEALLGLPFMELVFRAAEIHRQFFDPTKVQLSTLV
CCCHHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHE
SIKTGGCPEDCGYCPQSVHHDTPVADQPMMTVDEVVAAARQAKANGAGRFCMGAAWRGPK
EECCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCC
DADLQKTLDMVREVKALGLETCATFGLLRDGQAEQLKDAGLDYYNHNLDTAPDKYGDIIQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHCCCCCCCCHHHHHHHH
TREYEDRLDTLGKVRKAGLSVCCGGIVGMNETRRDRAGLIVQLANLDPQPESVPVNNLVQ
HCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCEEEEECCCCCCCCCCCHHHHHH
VVGTPLEGAERLDWTEFVRTIAVARITMPASYVRLSAGRREMDEATQALCFLAGANSIFY
HHCCCCCCHHCCCHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEE
GDKLLTTGNPDVVADQSLMAKLNLQPL
CCEEEECCCCCCEECHHHHHHCCCCCC
>Mature Secondary Structure
MHSATMVFKRAATPHPEKAEWSVDDVEALLGLPFMELVFRAAEIHRQFFDPTKVQLSTLV
CCCHHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHE
SIKTGGCPEDCGYCPQSVHHDTPVADQPMMTVDEVVAAARQAKANGAGRFCMGAAWRGPK
EECCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCC
DADLQKTLDMVREVKALGLETCATFGLLRDGQAEQLKDAGLDYYNHNLDTAPDKYGDIIQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHCCCCCCCCHHHHHHHH
TREYEDRLDTLGKVRKAGLSVCCGGIVGMNETRRDRAGLIVQLANLDPQPESVPVNNLVQ
HCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCEEEEECCCCCCCCCCCHHHHHH
VVGTPLEGAERLDWTEFVRTIAVARITMPASYVRLSAGRREMDEATQALCFLAGANSIFY
HHCCCCCCHHCCCHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEE
GDKLLTTGNPDVVADQSLMAKLNLQPL
CCEEEECCCCCCEECHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782