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The map label for this gene is ftsI [H]

Identifier: 34499804

GI number: 34499804

Start: 4690212

End: 4691963

Strand: Reverse

Name: ftsI [H]

Synonym: CV_4349

Alternate gene names: 34499804

Gene position: 4691963-4690212 (Counterclockwise)

Preceding gene: 34499805

Following gene: 34499803

Centisome position: 98.76

GC content: 68.04

Gene sequence:

>1752_bases
ATGCGCGCCGGTTCCAGCTACAGCGTCCGCACCCGCCCCGCGCCGGCCAGCCCCGCGCTGCGGATGACCAGCACGCGCGT
GCGCTGCGTGCTGCTGATGCTGGCGCTGCTGTTCCTGGCGCTGATCGGCCGCGCGGTCTATCTGCAGGTGGTGCAGCAGG
ACTTCTTGCAGAACCAGGGTGAGGCGCGTTTCCGCCGCACCCTGACGCTGGAGGCCAACCGCGGCGTGATCACCGACCGC
AACGGCGAGCCGCTGGCGATCAGCTCGCCGGTGCAGACCATCTGGGCCAGTCCGGCCGACATGGAGCCGGTGCCGCCGGC
CAAGCTGCGAGAGCTGGCCGGCCTGCTGGACCTGTCGCCGGAGGAGCTGTCGGCCAAGTTGTCTGACCGCAAGAAGGAAT
TCGTCTACATCAAGCGCCAGATCAGCCCGGAGCTGGCGGACAAGGTGATGGCGCTGGGCGTGCCCGGCATCGCCAAGCAG
CAGGAGTTCCGCCGCTTCTACCCGGCCGGCGAGATCGTGTCGCACATCATCGGTTTCACCGGGGTGGACGGCAAGGGCCA
GGAGGGCGTGGAGCTCGCCCGCGAGAAGATGCTGTCCGGCAAGGATGGCCGCCGCGTGGTGCTGAAGGATCGCCGCGGCC
ACATCATCGAGGACGTCGCCGCCATCGAGCCGCCGCGCGACGGCCAGAAGCTGGCGCTGGCGATCGACCACCGCATCCAG
TACCTGGCCTACCGCGAAATCAAGGCCGCGGTGGAGACCAACAAGGCCAAGGCCGGCAGCGTGGTGGTGCTGGACGCCCA
CACCGGCGAACTGCTGGCGCTGGCCAACTACCCGTCGTTCAACCCGAACAACCGCAGCGGCGTGACGCCGGAAATGATGC
GCAACCGCGGCGTGATCGACCTGTTCGAGCCCGGCTCGACGATGAAGCCGCTGTCGATCTCGCTGGCGCTGGAGCACGGC
AAGGCCAATCTGAACACGGTGCTGGACACCCACAGCTACATGATAGGCCCGGCCACCATCCGCGACGTGTCGCCGCAGGC
CAGCCTGGGCATCCTGGGCATCATCCAGAAATCGTCCAACGTCGGCACCAGCAAGCTGGCGCTGCTGAACAGCCCGGAAG
AGTTCTGGAAGCACTACGACGCGCTGGGCTTCGGCCGGGCGCCGGAAACCGGCTTCCCCGGCGAGGCCAGCGGCCGGCTG
CGCGACTGGCACAAGTGGCGCCCGATCGAGCAGGCGACGATGTCCTTCGGCTACGGCGTGTCGGTCAGCCTGATCCAGAT
GGCGCGCGCCTACACCATTTTCGCCAACGACGGCGTGATGCTGCCGGTGGCGCTGTACAAGACTGCCAACCCGATGCCGG
GCAAGCGGGTGCTCAGCGAGAAGACCGCGCACGAGATGCGCGATCTGCTGATCGCCAACAGCCAGCCTGGCGGCGGCGCC
GTCGGCGGCCGCATCATCGGCTACAGCATAGGCGGCAAGAGCGGCACCGCGCGCAAGCTGGAGGGCCGCGCCTACGTGGC
CAACAAGCACCGCGCGCTGTTCATGGGCTTCGCGCCCGGCCACTCGCCCAAGGTGATCGTGGCGGTGATGATAGACGAGC
CGTCCGCGGGCAAATACTACGGCGGCGCGGTGTCCGCGCCGGTGTTCTCTCAGGTGGCGGGCGGCGCGCTGCGCGTGCTG
AACATCGCGCCGGACGAGCCATCCAACAACACTTTATTGCCGGAATCGACCCCGGTTCCGGCGGATTTCTAA

Upstream 100 bases:

>100_bases
GCGCGCACGCGGTGATAGAAAAGGCCGCGTCCACCCGTCTCGACATGCACACCCCCGATCCGCGCCAGATCCAGGTGATC
ACGGCCAAGGGAGCGCTGTG

Downstream 100 bases:

>100_bases
GATCATGAAGAGCCGTTTGACTGCGTTGCCGGACTGGGATCCGGCCTTGTTGCAACAGCTGGGCCTCCCGATCAAGCGGG
TGGAGGCCGACAGCCGCCGC

Product: penicillin-binding protein 3

Products: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-; $diphosphoundecaprenol; undecaprenyl diphosphate

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 583; Mature: 583

Protein sequence:

>583_residues
MRAGSSYSVRTRPAPASPALRMTSTRVRCVLLMLALLFLALIGRAVYLQVVQQDFLQNQGEARFRRTLTLEANRGVITDR
NGEPLAISSPVQTIWASPADMEPVPPAKLRELAGLLDLSPEELSAKLSDRKKEFVYIKRQISPELADKVMALGVPGIAKQ
QEFRRFYPAGEIVSHIIGFTGVDGKGQEGVELAREKMLSGKDGRRVVLKDRRGHIIEDVAAIEPPRDGQKLALAIDHRIQ
YLAYREIKAAVETNKAKAGSVVVLDAHTGELLALANYPSFNPNNRSGVTPEMMRNRGVIDLFEPGSTMKPLSISLALEHG
KANLNTVLDTHSYMIGPATIRDVSPQASLGILGIIQKSSNVGTSKLALLNSPEEFWKHYDALGFGRAPETGFPGEASGRL
RDWHKWRPIEQATMSFGYGVSVSLIQMARAYTIFANDGVMLPVALYKTANPMPGKRVLSEKTAHEMRDLLIANSQPGGGA
VGGRIIGYSIGGKSGTARKLEGRAYVANKHRALFMGFAPGHSPKVIVAVMIDEPSAGKYYGGAVSAPVFSQVAGGALRVL
NIAPDEPSNNTLLPESTPVPADF

Sequences:

>Translated_583_residues
MRAGSSYSVRTRPAPASPALRMTSTRVRCVLLMLALLFLALIGRAVYLQVVQQDFLQNQGEARFRRTLTLEANRGVITDR
NGEPLAISSPVQTIWASPADMEPVPPAKLRELAGLLDLSPEELSAKLSDRKKEFVYIKRQISPELADKVMALGVPGIAKQ
QEFRRFYPAGEIVSHIIGFTGVDGKGQEGVELAREKMLSGKDGRRVVLKDRRGHIIEDVAAIEPPRDGQKLALAIDHRIQ
YLAYREIKAAVETNKAKAGSVVVLDAHTGELLALANYPSFNPNNRSGVTPEMMRNRGVIDLFEPGSTMKPLSISLALEHG
KANLNTVLDTHSYMIGPATIRDVSPQASLGILGIIQKSSNVGTSKLALLNSPEEFWKHYDALGFGRAPETGFPGEASGRL
RDWHKWRPIEQATMSFGYGVSVSLIQMARAYTIFANDGVMLPVALYKTANPMPGKRVLSEKTAHEMRDLLIANSQPGGGA
VGGRIIGYSIGGKSGTARKLEGRAYVANKHRALFMGFAPGHSPKVIVAVMIDEPSAGKYYGGAVSAPVFSQVAGGALRVL
NIAPDEPSNNTLLPESTPVPADF
>Mature_583_residues
MRAGSSYSVRTRPAPASPALRMTSTRVRCVLLMLALLFLALIGRAVYLQVVQQDFLQNQGEARFRRTLTLEANRGVITDR
NGEPLAISSPVQTIWASPADMEPVPPAKLRELAGLLDLSPEELSAKLSDRKKEFVYIKRQISPELADKVMALGVPGIAKQ
QEFRRFYPAGEIVSHIIGFTGVDGKGQEGVELAREKMLSGKDGRRVVLKDRRGHIIEDVAAIEPPRDGQKLALAIDHRIQ
YLAYREIKAAVETNKAKAGSVVVLDAHTGELLALANYPSFNPNNRSGVTPEMMRNRGVIDLFEPGSTMKPLSISLALEHG
KANLNTVLDTHSYMIGPATIRDVSPQASLGILGIIQKSSNVGTSKLALLNSPEEFWKHYDALGFGRAPETGFPGEASGRL
RDWHKWRPIEQATMSFGYGVSVSLIQMARAYTIFANDGVMLPVALYKTANPMPGKRVLSEKTAHEMRDLLIANSQPGGGA
VGGRIIGYSIGGKSGTARKLEGRAYVANKHRALFMGFAPGHSPKVIVAVMIDEPSAGKYYGGAVSAPVFSQVAGGALRVL
NIAPDEPSNNTLLPESTPVPADF

Specific function: Synthesis of cross-linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=542, Percent_Identity=43.7269372693727, Blast_Score=458, Evalue=1e-130,
Organism=Escherichia coli, GI1786854, Length=636, Percent_Identity=25.6289308176101, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: 2.4.1.129

Molecular weight: Translated: 63103; Mature: 63103

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAGSSYSVRTRPAPASPALRMTSTRVRCVLLMLALLFLALIGRAVYLQVVQQDFLQNQG
CCCCCCCEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EARFRRTLTLEANRGVITDRNGEPLAISSPVQTIWASPADMEPVPPAKLRELAGLLDLSP
CCEEEEEEEEECCCCEEECCCCCCEEECCCHHHHHCCCCCCCCCCHHHHHHHHHHHCCCH
EELSAKLSDRKKEFVYIKRQISPELADKVMALGVPGIAKQQEFRRFYPAGEIVSHIIGFT
HHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHCC
GVDGKGQEGVELAREKMLSGKDGRRVVLKDRRGHIIEDVAAIEPPRDGQKLALAIDHRIQ
CCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHCCCCCCCCEEEEEEHHHHH
YLAYREIKAAVETNKAKAGSVVVLDAHTGELLALANYPSFNPNNRSGVTPEMMRNRGVID
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCEE
LFEPGSTMKPLSISLALEHGKANLNTVLDTHSYMIGPATIRDVSPQASLGILGIIQKSSN
EECCCCCCCCEEEEEEEECCCCCCHHEEECCCEEECCHHEECCCCCCCCCEEEEEECCCC
VGTSKLALLNSPEEFWKHYDALGFGRAPETGFPGEASGRLRDWHKWRPIEQATMSFGYGV
CCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHCCCCC
SVSLIQMARAYTIFANDGVMLPVALYKTANPMPGKRVLSEKTAHEMRDLLIANSQPGGGA
HHHHHHHHHEEEEEECCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCC
VGGRIIGYSIGGKSGTARKLEGRAYVANKHRALFMGFAPGHSPKVIVAVMIDEPSAGKYY
CCCEEEEEEECCCCCCCEEECCCEEEECCCEEEEEEECCCCCCCEEEEEEECCCCCCCCC
GGAVSAPVFSQVAGGALRVLNIAPDEPSNNTLLPESTPVPADF
CCCCCCHHHHHHCCCEEEEEEECCCCCCCCEECCCCCCCCCCC
>Mature Secondary Structure
MRAGSSYSVRTRPAPASPALRMTSTRVRCVLLMLALLFLALIGRAVYLQVVQQDFLQNQG
CCCCCCCEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EARFRRTLTLEANRGVITDRNGEPLAISSPVQTIWASPADMEPVPPAKLRELAGLLDLSP
CCEEEEEEEEECCCCEEECCCCCCEEECCCHHHHHCCCCCCCCCCHHHHHHHHHHHCCCH
EELSAKLSDRKKEFVYIKRQISPELADKVMALGVPGIAKQQEFRRFYPAGEIVSHIIGFT
HHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHCC
GVDGKGQEGVELAREKMLSGKDGRRVVLKDRRGHIIEDVAAIEPPRDGQKLALAIDHRIQ
CCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHCCCCCCCCEEEEEEHHHHH
YLAYREIKAAVETNKAKAGSVVVLDAHTGELLALANYPSFNPNNRSGVTPEMMRNRGVID
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCEE
LFEPGSTMKPLSISLALEHGKANLNTVLDTHSYMIGPATIRDVSPQASLGILGIIQKSSN
EECCCCCCCCEEEEEEEECCCCCCHHEEECCCEEECCHHEECCCCCCCCCEEEEEECCCC
VGTSKLALLNSPEEFWKHYDALGFGRAPETGFPGEASGRLRDWHKWRPIEQATMSFGYGV
CCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHCCCCC
SVSLIQMARAYTIFANDGVMLPVALYKTANPMPGKRVLSEKTAHEMRDLLIANSQPGGGA
HHHHHHHHHEEEEEECCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCC
VGGRIIGYSIGGKSGTARKLEGRAYVANKHRALFMGFAPGHSPKVIVAVMIDEPSAGKYY
CCCEEEEEEECCCCCCCEEECCCEEEECCCEEEEEEECCCCCCCEEEEEEECCCCCCCCC
GGAVSAPVFSQVAGGALRVLNIAPDEPSNNTLLPESTPVPADF
CCCCCCHHHHHHCCCEEEEEEECCCCCCCCEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-; $diphosphoundecaprenol; GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-; $diphosphoundecaprenol

Specific reaction: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n- $diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu- $L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = [GlcNAc-(1->4)- $Mur2Ac(oyl-L-Ala-gamma-

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3126399 [H]