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The map label for this gene is murD

Identifier: 34499800

GI number: 34499800

Start: 4684909

End: 4686270

Strand: Reverse

Name: murD

Synonym: CV_4345

Alternate gene names: 34499800

Gene position: 4686270-4684909 (Counterclockwise)

Preceding gene: 34499801

Following gene: 34499799

Centisome position: 98.64

GC content: 70.12

Gene sequence:

>1362_bases
ATGGATTACGCCAATCGACATGTGACGGTGGTGGGATTGGGGGGCTCGGGCCTGGCGGCCGCGCGCTACCTGGCCGCCCA
CGGCGCGCGCGTGCGCGTGGCCGACGCCAATCCGTCCGCCGAGCGGCTGGCCGAGCTGGAACGCTGCCTGCCGGGCGTGG
AAGTGATGGTCGGCGCGTTCGACGACGCCACCTTCGCCGGCGCCGAGCTGCTGGTGGTCAGTCCCGGCGTGCCGCTGGCC
AACCCGGCCATCGCCGCCTTCCGCCGCGCCGGCGGCGAGGTGGTCGGCGACATCGAGATCCTGGCGCGCGCGATCCAGGG
CGACGGCAGCAAGGTGATCGCGATCACCGGCTCCAACGGCAAGAGCACGGTCACCAGTCTGGTCGGCCACTTGTGCGAGG
CGGCCGGGCTGGATACCGTCGTCGCCGGCAATATCGGCCTGGCGGTGCTGGAGGCTTTGCTCGCGCGCGAACAGAGCGGC
AAGCGCCCCGACGTGTGGGTGCTGGAGCTGTCCAGCTTCCAGCTGGAGTCCACTTTCTCGCTGGCGGCCGACGCCGCCAC
CGTGCTGAACATTTCCGAGGATCACCTCGACCGTTACGCCGACCTGCTGGACTACGCGCATGCCAAGACCCGGGTGTTCA
ACGGCAAGGGCGTCCAGGTGCTGAACAAGGACGACGCGCTGGTGCGGGCGATGGTCCGCCCCGGCCATCCGGTCAAGTGG
TTCTCGCTGAACGGCGCGGCAGACTACGCGCTGGCGCGCAACGGCGGCTACTGGCTGAAAGTGGACGGCGAAAAGGTGTT
CGACTGCGCCGACATGCAGCTGCAGGGCCTGCACAACGCCGCCAACGCGCTGGCCGCGTTGGGCCTGTGCCAAGGCATAG
GCCTGCCGCTGGAGAAGCTGCTGGACGGGCTGAAGACCTTCCGCGGCCTCGCGCATCGCGTCGAGCTGGTAGACGAATTC
GACGGTATTGCGTTTATCGATGATTCCAAGGGCACCAACGTCGGCGCGACCGAGGCCGCGCTGAACGGCATGACCCGCCA
GGTGGTGCTGATCGCCGGCGGCGACGGCAAGGGCCAGGACTTCGCGCCGCTGAAGCCGGCCTGCCAGCGCATCGCCCGCG
CGGTGCTGCTGATCGGCCGCGACGCCGGCCGGATCGAGGCCGCGCTGGAAGACAGCGGCCTGGCGCTGGAGCGCTGCGAC
ACGCTGGAGGAGGCTACCCGCCGCGCGGCGGCGCTGGCCCGTCCGGGCGACGTGGTGCTGCTGTCGCCGGCCTGCGCCAG
CCTGGACATGTTCAAGAATTACGCGCACCGGGCGCAGGTGTTCATCGACACCGTGGCGGCGGTGAAGGCGGCGCGGGCAT
GA

Upstream 100 bases:

>100_bases
GGCTGGAAGGAAACCCAGGTGGTGGTCCGCTTCTGGATCGTCACCATGATGCTGGTGCTGATCGGCCTGTCGACGCTGAA
GCTGCGCTGAGGACGGAAAA

Downstream 100 bases:

>100_bases
ACCACAATCCGGCGCGCCGCTACACGGCGATCACCGCTCTGGACGAGGCGCTGGCCTGGGCGCTGGCGCTGCTGCTGTCG
ATCAGCCTGGTGATGGTGTA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MDYANRHVTVVGLGGSGLAAARYLAAHGARVRVADANPSAERLAELERCLPGVEVMVGAFDDATFAGAELLVVSPGVPLA
NPAIAAFRRAGGEVVGDIEILARAIQGDGSKVIAITGSNGKSTVTSLVGHLCEAAGLDTVVAGNIGLAVLEALLAREQSG
KRPDVWVLELSSFQLESTFSLAADAATVLNISEDHLDRYADLLDYAHAKTRVFNGKGVQVLNKDDALVRAMVRPGHPVKW
FSLNGAADYALARNGGYWLKVDGEKVFDCADMQLQGLHNAANALAALGLCQGIGLPLEKLLDGLKTFRGLAHRVELVDEF
DGIAFIDDSKGTNVGATEAALNGMTRQVVLIAGGDGKGQDFAPLKPACQRIARAVLLIGRDAGRIEAALEDSGLALERCD
TLEEATRRAAALARPGDVVLLSPACASLDMFKNYAHRAQVFIDTVAAVKAARA

Sequences:

>Translated_453_residues
MDYANRHVTVVGLGGSGLAAARYLAAHGARVRVADANPSAERLAELERCLPGVEVMVGAFDDATFAGAELLVVSPGVPLA
NPAIAAFRRAGGEVVGDIEILARAIQGDGSKVIAITGSNGKSTVTSLVGHLCEAAGLDTVVAGNIGLAVLEALLAREQSG
KRPDVWVLELSSFQLESTFSLAADAATVLNISEDHLDRYADLLDYAHAKTRVFNGKGVQVLNKDDALVRAMVRPGHPVKW
FSLNGAADYALARNGGYWLKVDGEKVFDCADMQLQGLHNAANALAALGLCQGIGLPLEKLLDGLKTFRGLAHRVELVDEF
DGIAFIDDSKGTNVGATEAALNGMTRQVVLIAGGDGKGQDFAPLKPACQRIARAVLLIGRDAGRIEAALEDSGLALERCD
TLEEATRRAAALARPGDVVLLSPACASLDMFKNYAHRAQVFIDTVAAVKAARA
>Mature_453_residues
MDYANRHVTVVGLGGSGLAAARYLAAHGARVRVADANPSAERLAELERCLPGVEVMVGAFDDATFAGAELLVVSPGVPLA
NPAIAAFRRAGGEVVGDIEILARAIQGDGSKVIAITGSNGKSTVTSLVGHLCEAAGLDTVVAGNIGLAVLEALLAREQSG
KRPDVWVLELSSFQLESTFSLAADAATVLNISEDHLDRYADLLDYAHAKTRVFNGKGVQVLNKDDALVRAMVRPGHPVKW
FSLNGAADYALARNGGYWLKVDGEKVFDCADMQLQGLHNAANALAALGLCQGIGLPLEKLLDGLKTFRGLAHRVELVDEF
DGIAFIDDSKGTNVGATEAALNGMTRQVVLIAGGDGKGQDFAPLKPACQRIARAVLLIGRDAGRIEAALEDSGLALERCD
TLEEATRRAAALARPGDVVLLSPACASLDMFKNYAHRAQVFIDTVAAVKAARA

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=447, Percent_Identity=44.0715883668904, Blast_Score=308, Evalue=5e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_CHRVO (Q7NPZ7)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_904015.1
- ProteinModelPortal:   Q7NPZ7
- SMR:   Q7NPZ7
- GeneID:   2549704
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_4345
- NMPDR:   fig|243365.1.peg.4345
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- PhylomeDB:   Q7NPZ7
- ProtClustDB:   PRK04308
- BioCyc:   CVIO243365:CV_4345-MONOMER
- BRENDA:   6.3.2.9
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 47450; Mature: 47450

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYANRHVTVVGLGGSGLAAARYLAAHGARVRVADANPSAERLAELERCLPGVEVMVGAF
CCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEECC
DDATFAGAELLVVSPGVPLANPAIAAFRRAGGEVVGDIEILARAIQGDGSKVIAITGSNG
CCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCCEEEHHHHHHHHHCCCCCEEEEEECCCC
KSTVTSLVGHLCEAAGLDTVVAGNIGLAVLEALLAREQSGKRPDVWVLELSSFQLESTFS
HHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEHHHHH
LAADAATVLNISEDHLDRYADLLDYAHAKTRVFNGKGVQVLNKDDALVRAMVRPGHPVKW
HHHCCHHEEECCHHHHHHHHHHHHHHHHHHEEECCCCEEEECCCHHHHHHHHCCCCCEEE
FSLNGAADYALARNGGYWLKVDGEKVFDCADMQLQGLHNAANALAALGLCQGIGLPLEKL
EEECCCCCEEEEECCCEEEEECCCEEECHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LDGLKTFRGLAHRVELVDEFDGIAFIDDSKGTNVGATEAALNGMTRQVVLIAGGDGKGQD
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHCCCCEEEEEEECCCCCCCC
FAPLKPACQRIARAVLLIGRDAGRIEAALEDSGLALERCDTLEEATRRAAALARPGDVVL
CCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEE
LSPACASLDMFKNYAHRAQVFIDTVAAVKAARA
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDYANRHVTVVGLGGSGLAAARYLAAHGARVRVADANPSAERLAELERCLPGVEVMVGAF
CCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEECC
DDATFAGAELLVVSPGVPLANPAIAAFRRAGGEVVGDIEILARAIQGDGSKVIAITGSNG
CCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCCEEEHHHHHHHHHCCCCCEEEEEECCCC
KSTVTSLVGHLCEAAGLDTVVAGNIGLAVLEALLAREQSGKRPDVWVLELSSFQLESTFS
HHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEHHHHH
LAADAATVLNISEDHLDRYADLLDYAHAKTRVFNGKGVQVLNKDDALVRAMVRPGHPVKW
HHHCCHHEEECCHHHHHHHHHHHHHHHHHHEEECCCCEEEECCCHHHHHHHHCCCCCEEE
FSLNGAADYALARNGGYWLKVDGEKVFDCADMQLQGLHNAANALAALGLCQGIGLPLEKL
EEECCCCCEEEEECCCEEEEECCCEEECHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LDGLKTFRGLAHRVELVDEFDGIAFIDDSKGTNVGATEAALNGMTRQVVLIAGGDGKGQD
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHCCCCEEEEEEECCCCCCCC
FAPLKPACQRIARAVLLIGRDAGRIEAALEDSGLALERCDTLEEATRRAAALARPGDVVL
CCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEE
LSPACASLDMFKNYAHRAQVFIDTVAAVKAARA
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782