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The map label for this gene is ddl

Identifier: 34499796

GI number: 34499796

Start: 4680274

End: 4681185

Strand: Reverse

Name: ddl

Synonym: CV_4341

Alternate gene names: 34499796

Gene position: 4681185-4680274 (Counterclockwise)

Preceding gene: 34499797

Following gene: 34499795

Centisome position: 98.53

GC content: 64.8

Gene sequence:

>912_bases
ATGAAGCAGTACGGCAAGGTGGCGGTATTGATGGGCGGAAGCTCGTCGGAGCGCGAAGTGTCGCTGATGAGCGGCGCCGG
CGTGCTTTCGGCGCTGCGGTCCAAGGGCGTGGACGCCCACGGGTTCGATCCGTCCGAGAAACCGCTGTCGGCGTTGAAGG
AAGAAGGTTTCGATTGCGTGTTCAACATCCTGCACGGCCCGTTCGGCGAGGATGGCACGCTGCAGGGCGCGCTGGAAGCG
CTGGGCATGCCCTACACCGGTTGCGGCGTGATGGCTTCGGCCATCGCCATGGACAAGTGGCGCACCAAGCTGTTGTGGAA
GGGCGCCGGCCTGCCGATCCCGGCGTTCGAGCTGCTGGACGAGAACAGCGATTTCGACGCGATCGAGCGGCAGCTGGGCC
TGCCCATCTTCGTCAAGCCGTCGACCGAAGGTTCGAGCATCGGCGTGACCAAGGTGAAGCAGCCGGGGGAATTGCGCGCG
GCGTTCGAAGAGGCGCGCAAGTACGACAAGGTGGTGATCGCCGAGCAGTTCATCGGCGGCGGCGAATACACCTGCGCGGT
GATCGGCGAGACGGCTTATCCGACCATCAAGATCGAGCCGGCCACCGAGTACTACGACTACCAGGCCAAGTACTTCCGCG
ACGACACGGTGTACCGCTGCCCGTCCGGCCTCGCGCCGGAGGTGGAAGCCAGGGCGCGCGAGCTGGCGCTGAAGGCGTTC
AAGGTGCTGGGTTGCCGCGGCTGGAGCCGGGTGGACTTCCTGATGGACGAGGCGGGAGAGATCTACCTGCTGGAAGCGAA
TACCAGTCCGGGCATGACCAGCCACAGCCTGGTGCCGATGGCGGCGCGGGCGGAGGGCATCGCTTACGAGGACCTGTGTC
TGAAGGTATTGGATACGGTGCATGTGGGATAA

Upstream 100 bases:

>100_bases
GCCGGCTCGGTGGGCGCGGTGCCGGGCAAGGTGACGGCCCTGGCGGGCTGATCGGAATTTCCGTCCGCGCCGTGGGCGCG
GGCTTGTTGAGGGTAGATAG

Downstream 100 bases:

>100_bases
CCATCGGCTGCTCAGGAGCCTGGCCAATCTGATGCTGGGCGCCGCGGCGCTGATGCTGCTGTACGCGGGGGGATTCTGGC
TGACGCACGCGCCGGTGTTC

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase B; D-alanylalanine synthetase B

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MKQYGKVAVLMGGSSSEREVSLMSGAGVLSALRSKGVDAHGFDPSEKPLSALKEEGFDCVFNILHGPFGEDGTLQGALEA
LGMPYTGCGVMASAIAMDKWRTKLLWKGAGLPIPAFELLDENSDFDAIERQLGLPIFVKPSTEGSSIGVTKVKQPGELRA
AFEEARKYDKVVIAEQFIGGGEYTCAVIGETAYPTIKIEPATEYYDYQAKYFRDDTVYRCPSGLAPEVEARARELALKAF
KVLGCRGWSRVDFLMDEAGEIYLLEANTSPGMTSHSLVPMAARAEGIAYEDLCLKVLDTVHVG

Sequences:

>Translated_303_residues
MKQYGKVAVLMGGSSSEREVSLMSGAGVLSALRSKGVDAHGFDPSEKPLSALKEEGFDCVFNILHGPFGEDGTLQGALEA
LGMPYTGCGVMASAIAMDKWRTKLLWKGAGLPIPAFELLDENSDFDAIERQLGLPIFVKPSTEGSSIGVTKVKQPGELRA
AFEEARKYDKVVIAEQFIGGGEYTCAVIGETAYPTIKIEPATEYYDYQAKYFRDDTVYRCPSGLAPEVEARARELALKAF
KVLGCRGWSRVDFLMDEAGEIYLLEANTSPGMTSHSLVPMAARAEGIAYEDLCLKVLDTVHVG
>Mature_303_residues
MKQYGKVAVLMGGSSSEREVSLMSGAGVLSALRSKGVDAHGFDPSEKPLSALKEEGFDCVFNILHGPFGEDGTLQGALEA
LGMPYTGCGVMASAIAMDKWRTKLLWKGAGLPIPAFELLDENSDFDAIERQLGLPIFVKPSTEGSSIGVTKVKQPGELRA
AFEEARKYDKVVIAEQFIGGGEYTCAVIGETAYPTIKIEPATEYYDYQAKYFRDDTVYRCPSGLAPEVEARARELALKAF
KVLGCRGWSRVDFLMDEAGEIYLLEANTSPGMTSHSLVPMAARAEGIAYEDLCLKVLDTVHVG

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=300, Percent_Identity=52.3333333333333, Blast_Score=317, Evalue=9e-88,
Organism=Escherichia coli, GI1786579, Length=344, Percent_Identity=34.0116279069767, Blast_Score=185, Evalue=4e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDLB_CHRVO (Q7NQ01)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_904011.1
- ProteinModelPortal:   Q7NQ01
- SMR:   Q7NQ01
- GeneID:   2549665
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_4341
- NMPDR:   fig|243365.1.peg.4341
- HOGENOM:   HBG427092
- OMA:   RAFIILH
- PhylomeDB:   Q7NQ01
- ProtClustDB:   PRK01372
- BioCyc:   CVIO243365:CV_4341-MONOMER
- BRENDA:   6.3.2.4
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 32786; Mature: 32786

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYGKVAVLMGGSSSEREVSLMSGAGVLSALRSKGVDAHGFDPSEKPLSALKEEGFDCV
CCCCCCEEEEECCCCCCCEEEEHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHH
FNILHGPFGEDGTLQGALEALGMPYTGCGVMASAIAMDKWRTKLLWKGAGLPIPAFELLD
HHHHCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHC
ENSDFDAIERQLGLPIFVKPSTEGSSIGVTKVKQPGELRAAFEEARKYDKVVIAEQFIGG
CCCCHHHHHHHCCCEEEEECCCCCCCCCEEECCCCHHHHHHHHHHHHHHHEEEEHHHCCC
GEYTCAVIGETAYPTIKIEPATEYYDYQAKYFRDDTVYRCPSGLAPEVEARARELALKAF
CCEEEEEEECCCCCEEEECCCHHHHCCHHHEECCCCEEECCCCCCCHHHHHHHHHHHHHH
KVLGCRGWSRVDFLMDEAGEIYLLEANTSPGMTSHSLVPMAARAEGIAYEDLCLKVLDTV
HHHCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHH
HVG
CCC
>Mature Secondary Structure
MKQYGKVAVLMGGSSSEREVSLMSGAGVLSALRSKGVDAHGFDPSEKPLSALKEEGFDCV
CCCCCCEEEEECCCCCCCEEEEHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHH
FNILHGPFGEDGTLQGALEALGMPYTGCGVMASAIAMDKWRTKLLWKGAGLPIPAFELLD
HHHHCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHC
ENSDFDAIERQLGLPIFVKPSTEGSSIGVTKVKQPGELRAAFEEARKYDKVVIAEQFIGG
CCCCHHHHHHHCCCEEEEECCCCCCCCCEEECCCCHHHHHHHHHHHHHHHEEEEHHHCCC
GEYTCAVIGETAYPTIKIEPATEYYDYQAKYFRDDTVYRCPSGLAPEVEARARELALKAF
CCEEEEEEECCCCCEEEECCCHHHHCCHHHEECCCCEEECCCCCCCHHHHHHHHHHHHHH
KVLGCRGWSRVDFLMDEAGEIYLLEANTSPGMTSHSLVPMAARAEGIAYEDLCLKVLDTV
HHHCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHH
HVG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782