Click here to switch to the map view.

The map label for this gene is pyrC [H]

Identifier: 34499785

GI number: 34499785

Start: 4671861

End: 4672946

Strand: Reverse

Name: pyrC [H]

Synonym: CV_4330

Alternate gene names: 34499785

Gene position: 4672946-4671861 (Counterclockwise)

Preceding gene: 34499786

Following gene: 34499784

Centisome position: 98.36

GC content: 68.51

Gene sequence:

>1086_bases
ATGGCTTTCCATCGCCATTGTTTTATCCATCGTTACGGCAAGCCATCCACCATGCAGACTCTCACCCTGATCCGCCCCGA
CGACTGGCATTTGCACCTGCGCGACGACGCCGCGCTGGCCGCGGTGCTGCCCGACACCTGTCGCCAGATGGGCCGCGCCA
TCGTCATGCCCAACCTGAAGCCGCCGGTCACCACCGTGGCCGCCGCCGCCGCCTACCGCGAGCGCATTCTGGCCGCGCGC
CCGGCCGGCAGCGCCTTCGAGCCGCTGATGACGCTTTATCTGACCGATAAAACCAGCGCCGACGAAATCCGCAAGGCCAA
GGCCTGCGGCTTCGTCCACGGCGTCAAGCTGTACCCGGCCGGCGCCACCACCAATTCCGACCACGGCGTCACCGATATCG
CCCACGCGATGCCGGCGCTGGAGGCGATGGCCGAAGCCGGCATGCCGCTCTTGGTGCACGGCGAAGTCACCGACGCCGAC
ATCGACGTGTTCGACCGCGAGGCGGTGTTCATCGAGCGGGTGATGAAGCCCTTGCTGGCCAAGCTGCCGCAACTGAAGGT
GGTGTTCGAGCACATCACCACCCGAGAGGCGGCCGAGTTCGTCGCCGCCGCGCCGGCCAACGTCGCCGCCACCATCACCG
CCCATCACCTGCTGATGAACCGCAACGCGCTGTTTGTCGGCGGCATCCGCCCGCACCACTACTGTCTGCCGGTGCTCAAG
CGCGAGCTGCACCGCCAGGCGCTGGTGAAGGCGGCGACGTCGGGCTCGGCCAAGTTCTTTCTCGGCACCGACAGCGCGCC
GCACGCGAAGGGCGCCAAGGAGGCGGCTTGCGGCTGCGCCGGCATGTACACCGCCAACGCGGCGATCGAGCTCTACGCCG
AGGCTTTCGAGGCCGCCGGCGCGCTGGACAAGCTGGAGGCTTTCGCCAGCCTGAACGGCCCGGCTTTCTACGGCTTGCCG
GCCAACGGCGACCGCATCGAGCTGGTCAAGGAGAGTTGGACGGTGCCGGCCGAGCTGCCTTATCCCGGCGACAGCCTGGT
GCCGCTGCGCGCCGGCGACAGCGTCGCCTGGCGTCTGCGGCGCTGA

Upstream 100 bases:

>100_bases
AGCTTTCTGCCGCCAGACCGGCAACGGCCTGCTGGAATCGACCACCACCGGCGAAGGCAAGTTCCGGATGGTGCTCCGGC
GCAAGTAGACATTCCTGGCG

Downstream 100 bases:

>100_bases
CTCCGCATGAGATCGGAAAGCCGCCGTTTGAGGCGGCTTTTTCATTTTTAGCGCCGTGTGGCTTCAAGCTTGGCGGGCAA
GCTTTTCTTGTGCAGAAGGG

Product: dihydroorotase

Products: NA

Alternate protein names: DHOase [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MAFHRHCFIHRYGKPSTMQTLTLIRPDDWHLHLRDDAALAAVLPDTCRQMGRAIVMPNLKPPVTTVAAAAAYRERILAAR
PAGSAFEPLMTLYLTDKTSADEIRKAKACGFVHGVKLYPAGATTNSDHGVTDIAHAMPALEAMAEAGMPLLVHGEVTDAD
IDVFDREAVFIERVMKPLLAKLPQLKVVFEHITTREAAEFVAAAPANVAATITAHHLLMNRNALFVGGIRPHHYCLPVLK
RELHRQALVKAATSGSAKFFLGTDSAPHAKGAKEAACGCAGMYTANAAIELYAEAFEAAGALDKLEAFASLNGPAFYGLP
ANGDRIELVKESWTVPAELPYPGDSLVPLRAGDSVAWRLRR

Sequences:

>Translated_361_residues
MAFHRHCFIHRYGKPSTMQTLTLIRPDDWHLHLRDDAALAAVLPDTCRQMGRAIVMPNLKPPVTTVAAAAAYRERILAAR
PAGSAFEPLMTLYLTDKTSADEIRKAKACGFVHGVKLYPAGATTNSDHGVTDIAHAMPALEAMAEAGMPLLVHGEVTDAD
IDVFDREAVFIERVMKPLLAKLPQLKVVFEHITTREAAEFVAAAPANVAATITAHHLLMNRNALFVGGIRPHHYCLPVLK
RELHRQALVKAATSGSAKFFLGTDSAPHAKGAKEAACGCAGMYTANAAIELYAEAFEAAGALDKLEAFASLNGPAFYGLP
ANGDRIELVKESWTVPAELPYPGDSLVPLRAGDSVAWRLRR
>Mature_360_residues
AFHRHCFIHRYGKPSTMQTLTLIRPDDWHLHLRDDAALAAVLPDTCRQMGRAIVMPNLKPPVTTVAAAAAYRERILAARP
AGSAFEPLMTLYLTDKTSADEIRKAKACGFVHGVKLYPAGATTNSDHGVTDIAHAMPALEAMAEAGMPLLVHGEVTDADI
DVFDREAVFIERVMKPLLAKLPQLKVVFEHITTREAAEFVAAAPANVAATITAHHLLMNRNALFVGGIRPHHYCLPVLKR
ELHRQALVKAATSGSAKFFLGTDSAPHAKGAKEAACGCAGMYTANAAIELYAEAFEAAGALDKLEAFASLNGPAFYGLPA
NGDRIELVKESWTVPAELPYPGDSLVPLRAGDSVAWRLRR

Specific function: Pyrimidine biosynthesis; third step. [C]

COG id: COG0418

COG function: function code F; Dihydroorotase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787301, Length=343, Percent_Identity=57.4344023323615, Blast_Score=399, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6323452, Length=367, Percent_Identity=35.6948228882834, Blast_Score=186, Evalue=5e-48,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR004721
- InterPro:   IPR002195 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.2.3 [H]

Molecular weight: Translated: 38985; Mature: 38854

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: PS00483 DIHYDROOROTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFHRHCFIHRYGKPSTMQTLTLIRPDDWHLHLRDDAALAAVLPDTCRQMGRAIVMPNLK
CCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEECCCHHHHHCHHHHHHHCCEEECCCCC
PPVTTVAAAAAYRERILAARPAGSAFEPLMTLYLTDKTSADEIRKAKACGFVHGVKLYPA
CCHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEEEECCCCHHHHHHHHHCCCEECEEEEEC
GATTNSDHGVTDIAHAMPALEAMAEAGMPLLVHGEVTDADIDVFDREAVFIERVMKPLLA
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHCHHHHHHHHHHHHHHH
KLPQLKVVFEHITTREAAEFVAAAPANVAATITAHHLLMNRNALFVGGIRPHHYCLPVLK
HCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHH
RELHRQALVKAATSGSAKFFLGTDSAPHAKGAKEAACGCAGMYTANAAIELYAEAFEAAG
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHCCCCCHHHCCHHHHHHHHHHHHHC
ALDKLEAFASLNGPAFYGLPANGDRIELVKESWTVPAELPYPGDSLVPLRAGDSVAWRLR
CHHHHHHHHCCCCCEEECCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCEEEEC
R
C
>Mature Secondary Structure 
AFHRHCFIHRYGKPSTMQTLTLIRPDDWHLHLRDDAALAAVLPDTCRQMGRAIVMPNLK
CCCCEEEEEECCCCCCEEEEEEECCCCCEEEEECCCHHHHHCHHHHHHHCCEEECCCCC
PPVTTVAAAAAYRERILAARPAGSAFEPLMTLYLTDKTSADEIRKAKACGFVHGVKLYPA
CCHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEEEECCCCHHHHHHHHHCCCEECEEEEEC
GATTNSDHGVTDIAHAMPALEAMAEAGMPLLVHGEVTDADIDVFDREAVFIERVMKPLLA
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHCHHHHHHHHHHHHHHH
KLPQLKVVFEHITTREAAEFVAAAPANVAATITAHHLLMNRNALFVGGIRPHHYCLPVLK
HCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHH
RELHRQALVKAATSGSAKFFLGTDSAPHAKGAKEAACGCAGMYTANAAIELYAEAFEAAG
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHCCCCCHHHCCHHHHHHHHHHHHHC
ALDKLEAFASLNGPAFYGLPANGDRIELVKESWTVPAELPYPGDSLVPLRAGDSVAWRLR
CHHHHHHHHCCCCCEEECCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCEEEEC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA