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The map label for this gene is oppA [H]

Identifier: 34499784

GI number: 34499784

Start: 4669826

End: 4671439

Strand: Reverse

Name: oppA [H]

Synonym: CV_4329

Alternate gene names: 34499784

Gene position: 4671439-4669826 (Counterclockwise)

Preceding gene: 34499785

Following gene: 34499783

Centisome position: 98.32

GC content: 62.33

Gene sequence:

>1614_bases
ATGTCGAACACGCAATCGCTGCAGGTGATCGCCGCCGCCGTCGCGCTGGCCATGGCTTCCGGATCCGCGCTGGCCGCCAA
GGTGCCGGCCGGCACCAAGCTGGCCGCCAAGCAGGAACTGGTCCGCAATACCGGGTCCGAGCCGGAAACCCTGGATCCGG
CCGTCGCCGAGTCGGTGCCGGCCAATACCATTACCGCAGACCTGTTCGAGGGCCTGACCGCCGCCGACAACAACGGCAAG
ATCGTGCCCGGCGTGGCGGAGTCCTGGAAGCAGACCGACGCCACCACCTGGGTATTCCACCTGCGCAAGAACGCGAAATG
GTCCGACGGCCATCCGGTGACCGCCGATGAATTCGTTTACGGCTGGCAGCGTTTCCTCGATCCGAAAACCGCGTCCCCGT
ACTCCGAGACCTACGGCATCTTCATCCTGAACGGCAAGGAAATCGCCACCGGCAAGAAGAAGCCGGCCGAACTCGGCGTC
AAGGCGCTGGACAAGTACACGCTGGAAGTGAAGACCCCGTACCCGGTGCTGTTCCTGCCGGCCCTGGTGGCCAATACCCA
GTTCGCCCCCGCGCCCAAGGCCACCATCGACAAATACGGCAAGGATTGGGTGAAGCCGGGCAAGATGATCAGCAACGGCG
CCTACATGCTGAAAGACTGGCAGGTGAACAGCAAGGTGGTGGTCGCCAAGAACGCCAACTACTGGGACGCCAAGAGCGTG
GTGCTGAACCAGACCACCTACCTGCCGATCGAAGACTCCAACGCCGACGTGAAGTTGTATGAATCCGGCCAGAACGACTG
GGTGCTGAACCTGCCGCCGGGCTCCTTCGACAAGTACAAGGCGCAGTATCCGAAGGAAATCCGCAACGGCGAGCTGGCCG
CGCTGCGCTACTACTCGTTCAACAACAAGGATGCGCTGCTGAAAGACGTGCGCGTGCGCAAGGCGCTGTCCATGGCGATC
GACCGCGACATCCTGGCCAAGAAAGTGACCGCCGACGGCCAGAACCCGGCCTACAGCGTGATGGTGCACGGCGTGTCCGG
CGCCGATCCGTCCACTTACGACTGGGCGAAATGGCCGATGGCCAAGCGCGTGGACGAGGCCAAGAAGCTGCTGGCCCAGG
CCGGCGTGAAGCCGGGCACCAAGATCAAGTTCACCTACAACACCAACGACTACCATAAGAAGATGGCGATCTTCGCCGCT
TCCGAGTGGAAATCCAAGCTGGGCCTGAACACCGAACTGGAAAGCCTCGAGTTCAAGGTGCTGCTGAAGAAGCGCCATGA
CGGCGATTACCAGATCGCCCGCAACGGCTGGAACGCCGACTACAACGACGCCACCACCTTCCTGGCGCTGGTCGAGTGCG
GCAACACCCAGAACGACAACCAGAACTGCAACCCGAAGGCCCAAGAGCTGATCAAGCAGGGCAACCAGGCGACCGATGCC
GGCAAGCGCAAGCAGCTGTTCACCCAGGCCGTCAAGATGATCATGGACGACTATCCGATGCTGCCGCTGCTGCAGTACAC
GGCGCCGCGCCTGATCAAGTCCTATGTCGGCGGCGTGGGCCAGAACCCGCTGGACCGCTATCGCAGCAAGGACCTGTACA
TCATCCAGCACTGA

Upstream 100 bases:

>100_bases
CCTCTGCGCATAATCGTCGAACCCACTCTGGATACAATAGAGTGAAAAAATTCGAACAACCCGCCGGGCGCGGGATGCTA
CAGAATCAGGAGAATTAGAG

Downstream 100 bases:

>100_bases
GCGTGCCAAGCGAGACGCCGGCTGACGCCGGCGGCAACCGAGGCCGGATGTCCGCGCGAGCGGCCATCCGGCTTGTGATG
TAGGGGCTGGGCCGCGGTGC

Product: oligopeptide ABC transporter system, substrate-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MSNTQSLQVIAAAVALAMASGSALAAKVPAGTKLAAKQELVRNTGSEPETLDPAVAESVPANTITADLFEGLTAADNNGK
IVPGVAESWKQTDATTWVFHLRKNAKWSDGHPVTADEFVYGWQRFLDPKTASPYSETYGIFILNGKEIATGKKKPAELGV
KALDKYTLEVKTPYPVLFLPALVANTQFAPAPKATIDKYGKDWVKPGKMISNGAYMLKDWQVNSKVVVAKNANYWDAKSV
VLNQTTYLPIEDSNADVKLYESGQNDWVLNLPPGSFDKYKAQYPKEIRNGELAALRYYSFNNKDALLKDVRVRKALSMAI
DRDILAKKVTADGQNPAYSVMVHGVSGADPSTYDWAKWPMAKRVDEAKKLLAQAGVKPGTKIKFTYNTNDYHKKMAIFAA
SEWKSKLGLNTELESLEFKVLLKKRHDGDYQIARNGWNADYNDATTFLALVECGNTQNDNQNCNPKAQELIKQGNQATDA
GKRKQLFTQAVKMIMDDYPMLPLLQYTAPRLIKSYVGGVGQNPLDRYRSKDLYIIQH

Sequences:

>Translated_537_residues
MSNTQSLQVIAAAVALAMASGSALAAKVPAGTKLAAKQELVRNTGSEPETLDPAVAESVPANTITADLFEGLTAADNNGK
IVPGVAESWKQTDATTWVFHLRKNAKWSDGHPVTADEFVYGWQRFLDPKTASPYSETYGIFILNGKEIATGKKKPAELGV
KALDKYTLEVKTPYPVLFLPALVANTQFAPAPKATIDKYGKDWVKPGKMISNGAYMLKDWQVNSKVVVAKNANYWDAKSV
VLNQTTYLPIEDSNADVKLYESGQNDWVLNLPPGSFDKYKAQYPKEIRNGELAALRYYSFNNKDALLKDVRVRKALSMAI
DRDILAKKVTADGQNPAYSVMVHGVSGADPSTYDWAKWPMAKRVDEAKKLLAQAGVKPGTKIKFTYNTNDYHKKMAIFAA
SEWKSKLGLNTELESLEFKVLLKKRHDGDYQIARNGWNADYNDATTFLALVECGNTQNDNQNCNPKAQELIKQGNQATDA
GKRKQLFTQAVKMIMDDYPMLPLLQYTAPRLIKSYVGGVGQNPLDRYRSKDLYIIQH
>Mature_536_residues
SNTQSLQVIAAAVALAMASGSALAAKVPAGTKLAAKQELVRNTGSEPETLDPAVAESVPANTITADLFEGLTAADNNGKI
VPGVAESWKQTDATTWVFHLRKNAKWSDGHPVTADEFVYGWQRFLDPKTASPYSETYGIFILNGKEIATGKKKPAELGVK
ALDKYTLEVKTPYPVLFLPALVANTQFAPAPKATIDKYGKDWVKPGKMISNGAYMLKDWQVNSKVVVAKNANYWDAKSVV
LNQTTYLPIEDSNADVKLYESGQNDWVLNLPPGSFDKYKAQYPKEIRNGELAALRYYSFNNKDALLKDVRVRKALSMAID
RDILAKKVTADGQNPAYSVMVHGVSGADPSTYDWAKWPMAKRVDEAKKLLAQAGVKPGTKIKFTYNTNDYHKKMAIFAAS
EWKSKLGLNTELESLEFKVLLKKRHDGDYQIARNGWNADYNDATTFLALVECGNTQNDNQNCNPKAQELIKQGNQATDAG
KRKQLFTQAVKMIMDDYPMLPLLQYTAPRLIKSYVGGVGQNPLDRYRSKDLYIIQH

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=545, Percent_Identity=44.4036697247706, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI87081878, Length=537, Percent_Identity=38.9199255121043, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1789397, Length=530, Percent_Identity=39.0566037735849, Blast_Score=346, Evalue=2e-96,
Organism=Escherichia coli, GI1789966, Length=547, Percent_Identity=25.4113345521024, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1787762, Length=521, Percent_Identity=25.7197696737044, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1787052, Length=513, Percent_Identity=22.6120857699805, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59482; Mature: 59351

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTQSLQVIAAAVALAMASGSALAAKVPAGTKLAAKQELVRNTGSEPETLDPAVAESVP
CCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHCCC
ANTITADLFEGLTAADNNGKIVPGVAESWKQTDATTWVFHLRKNAKWSDGHPVTADEFVY
CCHHHHHHHCCCEECCCCCEECCCHHHHHHHCCCEEEEEEEECCCCCCCCCCCCHHHHHH
GWQRFLDPKTASPYSETYGIFILNGKEIATGKKKPAELGVKALDKYTLEVKTPYPVLFLP
HHHHHCCCCCCCCCCCCCEEEEECCCEECCCCCCCHHHHHHHHCCEEEEECCCCCHHHHH
ALVANTQFAPAPKATIDKYGKDWVKPGKMISNGAYMLKDWQVNSKVVVAKNANYWDAKSV
HHHHCCCCCCCCCHHHHHHCHHHCCCCCEECCCEEEEEEEECCCEEEEEECCCCCCCCEE
VLNQTTYLPIEDSNADVKLYESGQNDWVLNLPPGSFDKYKAQYPKEIRNGELAALRYYSF
EEECEEEEEEECCCCCEEEEECCCCCEEEECCCCCCHHHHHHCCHHHCCCCEEEEEEEEC
NNKDALLKDVRVRKALSMAIDRDILAKKVTADGQNPAYSVMVHGVSGADPSTYDWAKWPM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCHHHCCH
AKRVDEAKKLLAQAGVKPGTKIKFTYNTNDYHKKMAIFAASEWKSKLGLNTELESLEFKV
HHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHEEEEEEHHHHHHHCCCCCCHHHHHEEE
LLKKRHDGDYQIARNGWNADYNDATTFLALVECGNTQNDNQNCNPKAQELIKQGNQATDA
EEECCCCCCEEEEECCCCCCCCHHHHEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCC
GKRKQLFTQAVKMIMDDYPMLPLLQYTAPRLIKSYVGGVGQNPLDRYRSKDLYIIQH
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEEEEC
>Mature Secondary Structure 
SNTQSLQVIAAAVALAMASGSALAAKVPAGTKLAAKQELVRNTGSEPETLDPAVAESVP
CCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHCCC
ANTITADLFEGLTAADNNGKIVPGVAESWKQTDATTWVFHLRKNAKWSDGHPVTADEFVY
CCHHHHHHHCCCEECCCCCEECCCHHHHHHHCCCEEEEEEEECCCCCCCCCCCCHHHHHH
GWQRFLDPKTASPYSETYGIFILNGKEIATGKKKPAELGVKALDKYTLEVKTPYPVLFLP
HHHHHCCCCCCCCCCCCCEEEEECCCEECCCCCCCHHHHHHHHCCEEEEECCCCCHHHHH
ALVANTQFAPAPKATIDKYGKDWVKPGKMISNGAYMLKDWQVNSKVVVAKNANYWDAKSV
HHHHCCCCCCCCCHHHHHHCHHHCCCCCEECCCEEEEEEEECCCEEEEEECCCCCCCCEE
VLNQTTYLPIEDSNADVKLYESGQNDWVLNLPPGSFDKYKAQYPKEIRNGELAALRYYSF
EEECEEEEEEECCCCCEEEEECCCCCEEEECCCCCCHHHHHHCCHHHCCCCEEEEEEEEC
NNKDALLKDVRVRKALSMAIDRDILAKKVTADGQNPAYSVMVHGVSGADPSTYDWAKWPM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCHHHCCH
AKRVDEAKKLLAQAGVKPGTKIKFTYNTNDYHKKMAIFAASEWKSKLGLNTELESLEFKV
HHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHEEEEEEHHHHHHHCCCCCCHHHHHEEE
LLKKRHDGDYQIARNGWNADYNDATTFLALVECGNTQNDNQNCNPKAQELIKQGNQATDA
EEECCCCCCEEEEECCCCCCCCHHHHEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCC
GKRKQLFTQAVKMIMDDYPMLPLLQYTAPRLIKSYVGGVGQNPLDRYRSKDLYIIQH
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187863; 9097039; 9278503; 2015910; 9298646; 9600841 [H]