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The map label for this gene is puuA [H]

Identifier: 34499753

GI number: 34499753

Start: 4639058

End: 4640404

Strand: Direct

Name: puuA [H]

Synonym: CV_4298

Alternate gene names: 34499753

Gene position: 4639058-4640404 (Clockwise)

Preceding gene: 34499752

Following gene: 34499755

Centisome position: 97.64

GC content: 69.04

Gene sequence:

>1347_bases
ATGTCCGAAGCCCGCATGCCCGCAGGCCGAGCCGAATGGCTGCAACACAGCAAGGTGCGAGACGTCGAGCTCGCCTTTGC
CGATGTCCATGGTTTTCCCCGCGGCAAGACCCTGCCCGCCGCCGCCTTCATCCAAGGGCAGCCGCTGCGCATCGCGCGCG
CGGTGCCGCTGCAAAGCTGCGTCGGCGAATTCCCCGACTACAGCTTTTACGGCGAGCACGATCCGGACGTCACGCTGGCG
CCGGACTACGACACGCTGCGGCCGATGCCCTGGGCCAAGACTCCGCGCGCGATGGTGATCTGCGACTGCGTCGACCACGA
CGGCGGCCTGTCGCCGCTAGCGCCGCGCTCGGTGCTGAAGCAAGTGCTGGCGCGCTACCGCGCCCACGGCTGGGCGCCGG
TGGTGGCGCCGGAGCTCGAGTTCTACCTGTTCGCCGCCCACGGCGACCCGGCGCAGCCATTCCAGCCGCCCCAGCTTGCC
AGCGGCCGCCGCGAGGCCGGCTTCGACGCCTTCGGCTTCTCCGCGCTGGGCGAACTGGAGGCGTTCTTCGACGAGCTGTA
CCAGGCCTGCGAAACGCTGGAGATCGCCACCGACACCTGCGTGCACGAGATGGGCCCCAGCCAGTTCGAGATCAACCTCA
AGCACGGCGACGCGCTGAAGCTGGCCGACGATACCTTCCTGTTCAAGACCGCGCTGAAGGAAATCGCCCACCGCCACGGC
CTCAGCGCCGTGTGCATGGCCAAGCCGCTGCCCGGCCAGCCCGGCAGCTCGATGCACATCCACCAGAGCGTGGTGGACCG
CCAGGGCCGCAACGCCTTCAGCCGCGCCGACGGCTCGGAAAGCCCGGAATTCTTCGGCTTCATCGCCGGCCTGCAGCGCT
TCCTGCCCGAACTGATGCCGATGTTCTGTCCCAATCCCAACTCCTACCGCCGCTTCGTCAAGGGCATGGCGGCGCCGGTC
AACCTCAGTTGGGGGCTGGACAACCGCTCGGTCGGCCTCAGGGTGCCGGTATCCGGCCCCGAGGCGCGGCGGGTGGAAAA
CCGCCTGCCCGGCTGCGACGCCAATCCCTATCTGGCGCTGGCCGCCAGCCTGGGCGCCGGCCTCGCCGGCATAGAGGGTA
AGCTGGTGCCGGACGAGGCGGTGCACGGCAATGTGTTCAAGCAGCAGGCCGCCAGCCTGCCGACCACGCTGGACGCCGCC
TGCGCGGCGATGGCCGCCGGCTGCGCCGAGGAGCTGTTCGGCGCCGACTTCAGCCGCGCCTACCTGGCGGTGAAGGAGGT
GGAGCTGAACGACTACCATCGCCAGGTCACCGCCTGGGAACGGCAATATCTGGGGTTTCTGGTCTAA

Upstream 100 bases:

>100_bases
CGCCGTTGCGGCTGGCATAATGGCAACGGGCACATGCTCCATGACAGCAAAAAAAACACCGAGGACGGACAGGAATCCCG
CAAGCTCAGTGGAGAAACGT

Downstream 100 bases:

>100_bases
AGCGTCCCAACGTTGATTGTTTGTCGACAGGCGCCACATCCCCTTCGGCCCGTGCCGGCAAAGCAGCCTGGCTCCAGGGG
CCTTTAAGCCGCCGTTTTTT

Product: glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSEARMPAGRAEWLQHSKVRDVELAFADVHGFPRGKTLPAAAFIQGQPLRIARAVPLQSCVGEFPDYSFYGEHDPDVTLA
PDYDTLRPMPWAKTPRAMVICDCVDHDGGLSPLAPRSVLKQVLARYRAHGWAPVVAPELEFYLFAAHGDPAQPFQPPQLA
SGRREAGFDAFGFSALGELEAFFDELYQACETLEIATDTCVHEMGPSQFEINLKHGDALKLADDTFLFKTALKEIAHRHG
LSAVCMAKPLPGQPGSSMHIHQSVVDRQGRNAFSRADGSESPEFFGFIAGLQRFLPELMPMFCPNPNSYRRFVKGMAAPV
NLSWGLDNRSVGLRVPVSGPEARRVENRLPGCDANPYLALAASLGAGLAGIEGKLVPDEAVHGNVFKQQAASLPTTLDAA
CAAMAAGCAEELFGADFSRAYLAVKEVELNDYHRQVTAWERQYLGFLV

Sequences:

>Translated_448_residues
MSEARMPAGRAEWLQHSKVRDVELAFADVHGFPRGKTLPAAAFIQGQPLRIARAVPLQSCVGEFPDYSFYGEHDPDVTLA
PDYDTLRPMPWAKTPRAMVICDCVDHDGGLSPLAPRSVLKQVLARYRAHGWAPVVAPELEFYLFAAHGDPAQPFQPPQLA
SGRREAGFDAFGFSALGELEAFFDELYQACETLEIATDTCVHEMGPSQFEINLKHGDALKLADDTFLFKTALKEIAHRHG
LSAVCMAKPLPGQPGSSMHIHQSVVDRQGRNAFSRADGSESPEFFGFIAGLQRFLPELMPMFCPNPNSYRRFVKGMAAPV
NLSWGLDNRSVGLRVPVSGPEARRVENRLPGCDANPYLALAASLGAGLAGIEGKLVPDEAVHGNVFKQQAASLPTTLDAA
CAAMAAGCAEELFGADFSRAYLAVKEVELNDYHRQVTAWERQYLGFLV
>Mature_447_residues
SEARMPAGRAEWLQHSKVRDVELAFADVHGFPRGKTLPAAAFIQGQPLRIARAVPLQSCVGEFPDYSFYGEHDPDVTLAP
DYDTLRPMPWAKTPRAMVICDCVDHDGGLSPLAPRSVLKQVLARYRAHGWAPVVAPELEFYLFAAHGDPAQPFQPPQLAS
GRREAGFDAFGFSALGELEAFFDELYQACETLEIATDTCVHEMGPSQFEINLKHGDALKLADDTFLFKTALKEIAHRHGL
SAVCMAKPLPGQPGSSMHIHQSVVDRQGRNAFSRADGSESPEFFGFIAGLQRFLPELMPMFCPNPNSYRRFVKGMAAPVN
LSWGLDNRSVGLRVPVSGPEARRVENRLPGCDANPYLALAASLGAGLAGIEGKLVPDEAVHGNVFKQQAASLPTTLDAAC
AAMAAGCAEELFGADFSRAYLAVKEVELNDYHRQVTAWERQYLGFLV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=470, Percent_Identity=24.468085106383, Blast_Score=119, Evalue=4e-27,
Organism=Escherichia coli, GI87081870, Length=429, Percent_Identity=32.1678321678322, Blast_Score=214, Evalue=8e-57,
Organism=Escherichia coli, GI1790301, Length=434, Percent_Identity=28.5714285714286, Blast_Score=136, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 48898; Mature: 48767

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEARMPAGRAEWLQHSKVRDVELAFADVHGFPRGKTLPAAAFIQGQPLRIARAVPLQSC
CCCCCCCCCHHHHHHHCCCCHHEEEEEHHCCCCCCCCCCHHHEECCCCEEHHHHCCHHHH
VGEFPDYSFYGEHDPDVTLAPDYDTLRPMPWAKTPRAMVICDCVDHDGGLSPLAPRSVLK
HHCCCCCCCCCCCCCCEEECCCCHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHH
QVLARYRAHGWAPVVAPELEFYLFAAHGDPAQPFQPPQLASGRREAGFDAFGFSALGELE
HHHHHHHHCCCCCEECCCEEEEEEEECCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHH
AFFDELYQACETLEIATDTCVHEMGPSQFEINLKHGDALKLADDTFLFKTALKEIAHRHG
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCHHHHHHHHHHHHHHHCC
LSAVCMAKPLPGQPGSSMHIHQSVVDRQGRNAFSRADGSESPEFFGFIAGLQRFLPELMP
CCEEEECCCCCCCCCCCCCHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
MFCPNPNSYRRFVKGMAAPVNLSWGLDNRSVGLRVPVSGPEARRVENRLPGCDANPYLAL
HHCCCCHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCHHHHHHHCCCCCCCCCHHHH
AASLGAGLAGIEGKLVPDEAVHGNVFKQQAASLPTTLDAACAAMAAGCAEELFGADFSRA
HHHHCCCCCCCCCCCCCCHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
YLAVKEVELNDYHRQVTAWERQYLGFLV
EEEEEEECHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEARMPAGRAEWLQHSKVRDVELAFADVHGFPRGKTLPAAAFIQGQPLRIARAVPLQSC
CCCCCCCCHHHHHHHCCCCHHEEEEEHHCCCCCCCCCCHHHEECCCCEEHHHHCCHHHH
VGEFPDYSFYGEHDPDVTLAPDYDTLRPMPWAKTPRAMVICDCVDHDGGLSPLAPRSVLK
HHCCCCCCCCCCCCCCEEECCCCHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHH
QVLARYRAHGWAPVVAPELEFYLFAAHGDPAQPFQPPQLASGRREAGFDAFGFSALGELE
HHHHHHHHCCCCCEECCCEEEEEEEECCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHH
AFFDELYQACETLEIATDTCVHEMGPSQFEINLKHGDALKLADDTFLFKTALKEIAHRHG
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCHHHHHHHHHHHHHHHCC
LSAVCMAKPLPGQPGSSMHIHQSVVDRQGRNAFSRADGSESPEFFGFIAGLQRFLPELMP
CCEEEECCCCCCCCCCCCCHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
MFCPNPNSYRRFVKGMAAPVNLSWGLDNRSVGLRVPVSGPEARRVENRLPGCDANPYLAL
HHCCCCHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCHHHHHHHCCCCCCCCCHHHH
AASLGAGLAGIEGKLVPDEAVHGNVFKQQAASLPTTLDAACAAMAAGCAEELFGADFSRA
HHHHCCCCCCCCCCCCCCHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
YLAVKEVELNDYHRQVTAWERQYLGFLV
EEEEEEECHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]