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The map label for this gene is gshB

Identifier: 34499730

GI number: 34499730

Start: 4608528

End: 4609472

Strand: Reverse

Name: gshB

Synonym: CV_4275

Alternate gene names: 34499730

Gene position: 4609472-4608528 (Counterclockwise)

Preceding gene: 34499731

Following gene: 34499729

Centisome position: 97.02

GC content: 67.41

Gene sequence:

>945_bases
ATGCGCATCCTGTTCATTGCCGACCCGCTGGAACACTTCAAGATTTACAAGGACACCACCTTCGCGATGATGGAGGAGGC
GCACCGCCGCGGCCACGCGATCAGCTTCGCCGAGGCCCACCAGCTGTCGGTCGAGGGCGGCCGCCTGCAGGTGGACGCCT
GCGACCTCGCCGTCACCGACGAACGCCCTGACTGGTACAAGCTCGGCCCGAAGCTGCGCCAGCCCTTGACCGCGTTCAGC
GCGGTGGTGATGCGCAAGGACCCGCCGTTCGACATGGAGTACCTGTACGCGTCGCAGCTGTTCACGCTGGCCGAGGCGCA
GGGCGTCAAGGTGTTCACCAGCGGCCAGGCGCTGCGGGACTTCAACGAGAAGCTGGCCATCCTGCACTTCCCGGAACTGA
TCTCGCCGACGCTGATCAGCGGCGAGGCCCACCGGCTGCGCGCCTTCGTCAACCAGCACCAGGACGTGATCCTGAAGCCG
CTGGACAGCATGGGCGGCGACAGCATCTTCCGCGTCAAGCCGGATGACCCCAACCTGTCGGTGATCATCGAAACCATCAC
CTGGCGCGGCAAGCGCACGGTGATGGCCCAGCGCTACATCCCGGAGATCCGCGACGGCGACAAGCGCATCCTGGTGATAG
ACGGCGTGCCGGTCGACCATTGCCTGGCGCGCATTCCGGCGGCGGGCGAGACGCGCGGCAATCTGGCGGCCGGCGGCCGC
GGCGAGGCCAGGCCCCTGAGCGCGCGCGACCGCGAGATCGCCGAGACCGTGGGGCCGGAGTTGAAGAAGCGCGGCATCCT
GCTGGCCGGACTGGACGTGATCGGCAACTACCTGACCGAGGTCAACGTCACCAGCCCGACCTGCTTCCGCGAAATCATGG
ACCAGACCGGGGTCAACGTCGCCGGCCTGTACATCGACGCGCTGGAGCGGCGCTGCCAGCACTGA

Upstream 100 bases:

>100_bases
CTACGCCTACGGCGTGATCTCGCGGCTGGCGCTGCTGGCGGCTTCGCTGGAGCTGGAAGCCACCGACCCGGACGCTGAAT
AACCCCGCTGGAGCACAGAC

Downstream 100 bases:

>100_bases
GCGCTTGGCTGCGCGGCGCATGCGCGCTACCATGCCGGTCTTTTGAAAGCCGGTGCAGCCGGATTGTGGAGCGAGGATGG
GAAAACAGCAGGAAAGCCCG

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MRILFIADPLEHFKIYKDTTFAMMEEAHRRGHAISFAEAHQLSVEGGRLQVDACDLAVTDERPDWYKLGPKLRQPLTAFS
AVVMRKDPPFDMEYLYASQLFTLAEAQGVKVFTSGQALRDFNEKLAILHFPELISPTLISGEAHRLRAFVNQHQDVILKP
LDSMGGDSIFRVKPDDPNLSVIIETITWRGKRTVMAQRYIPEIRDGDKRILVIDGVPVDHCLARIPAAGETRGNLAAGGR
GEARPLSARDREIAETVGPELKKRGILLAGLDVIGNYLTEVNVTSPTCFREIMDQTGVNVAGLYIDALERRCQH

Sequences:

>Translated_314_residues
MRILFIADPLEHFKIYKDTTFAMMEEAHRRGHAISFAEAHQLSVEGGRLQVDACDLAVTDERPDWYKLGPKLRQPLTAFS
AVVMRKDPPFDMEYLYASQLFTLAEAQGVKVFTSGQALRDFNEKLAILHFPELISPTLISGEAHRLRAFVNQHQDVILKP
LDSMGGDSIFRVKPDDPNLSVIIETITWRGKRTVMAQRYIPEIRDGDKRILVIDGVPVDHCLARIPAAGETRGNLAAGGR
GEARPLSARDREIAETVGPELKKRGILLAGLDVIGNYLTEVNVTSPTCFREIMDQTGVNVAGLYIDALERRCQH
>Mature_314_residues
MRILFIADPLEHFKIYKDTTFAMMEEAHRRGHAISFAEAHQLSVEGGRLQVDACDLAVTDERPDWYKLGPKLRQPLTAFS
AVVMRKDPPFDMEYLYASQLFTLAEAQGVKVFTSGQALRDFNEKLAILHFPELISPTLISGEAHRLRAFVNQHQDVILKP
LDSMGGDSIFRVKPDDPNLSVIIETITWRGKRTVMAQRYIPEIRDGDKRILVIDGVPVDHCLARIPAAGETRGNLAAGGR
GEARPLSARDREIAETVGPELKKRGILLAGLDVIGNYLTEVNVTSPTCFREIMDQTGVNVAGLYIDALERRCQH

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1789316, Length=314, Percent_Identity=48.0891719745223, Blast_Score=314, Evalue=5e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSHB_CHRVO (Q7NQ65)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903945.1
- ProteinModelPortal:   Q7NQ65
- SMR:   Q7NQ65
- GeneID:   2548852
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_4275
- NMPDR:   fig|243365.1.peg.4275
- HOGENOM:   HBG624526
- OMA:   WMRKDPP
- PhylomeDB:   Q7NQ65
- ProtClustDB:   PRK05246
- BioCyc:   CVIO243365:CV_4275-MONOMER
- BRENDA:   6.3.2.3
- HAMAP:   MF_00162
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01380

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.3

Molecular weight: Translated: 35088; Mature: 35088

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILFIADPLEHFKIYKDTTFAMMEEAHRRGHAISFAEAHQLSVEGGRLQVDACDLAVTD
CEEEEEECCHHHHEECCCHHHHHHHHHHHCCCEEEEHHHHEEEECCCEEEEEEEEEEEEC
ERPDWYKLGPKLRQPLTAFSAVVMRKDPPFDMEYLYASQLFTLAEAQGVKVFTSGQALRD
CCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHH
FNEKLAILHFPELISPTLISGEAHRLRAFVNQHQDVILKPLDSMGGDSIFRVKPDDPNLS
HHHCEEEEECHHHHCCEEECCCHHHHHHHHHCCHHHEEHHHHHCCCCCEEEECCCCCCEE
VIIETITWRGKRTVMAQRYIPEIRDGDKRILVIDGVPVDHCLARIPAAGETRGNLAAGGR
EEEEEEECCCCHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCC
GEARPLSARDREIAETVGPELKKRGILLAGLDVIGNYLTEVNVTSPTCFREIMDQTGVNV
CCCCCCCCCHHHHHHHHCHHHHHCCEEEECHHHHHHHHHEECCCCHHHHHHHHHHCCCEE
AGLYIDALERRCQH
EHHHHHHHHHHHCC
>Mature Secondary Structure
MRILFIADPLEHFKIYKDTTFAMMEEAHRRGHAISFAEAHQLSVEGGRLQVDACDLAVTD
CEEEEEECCHHHHEECCCHHHHHHHHHHHCCCEEEEHHHHEEEECCCEEEEEEEEEEEEC
ERPDWYKLGPKLRQPLTAFSAVVMRKDPPFDMEYLYASQLFTLAEAQGVKVFTSGQALRD
CCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHH
FNEKLAILHFPELISPTLISGEAHRLRAFVNQHQDVILKPLDSMGGDSIFRVKPDDPNLS
HHHCEEEEECHHHHCCEEECCCHHHHHHHHHCCHHHEEHHHHHCCCCCEEEECCCCCCEE
VIIETITWRGKRTVMAQRYIPEIRDGDKRILVIDGVPVDHCLARIPAAGETRGNLAAGGR
EEEEEEECCCCHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCC
GEARPLSARDREIAETVGPELKKRGILLAGLDVIGNYLTEVNVTSPTCFREIMDQTGVNV
CCCCCCCCCHHHHHHHHCHHHHHCCEEEECHHHHHHHHHEECCCCHHHHHHHHHHCCCEE
AGLYIDALERRCQH
EHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782