Click here to switch to the map view.

The map label for this gene is 34499731

Identifier: 34499731

GI number: 34499731

Start: 4609491

End: 4610777

Strand: Reverse

Name: 34499731

Synonym: CV_4276

Alternate gene names: NA

Gene position: 4610777-4609491 (Counterclockwise)

Preceding gene: 34499732

Following gene: 34499730

Centisome position: 97.05

GC content: 66.05

Gene sequence:

>1287_bases
ATGCCACCCGTTCCGCACCTGGCCACCGCTCTCACCGGTCCGCTTTACGAGCTGGAAAGCCGCATCCTGGCGGCCCAGCC
GCAGATCGAGCACTGGTTCCGCAGCCAATGGCATGCCCACGCCACGCCGTTCTACGGCTCGGTGGACCTGCGCAACAGCG
GCTTCAAGCTGGCGCCGGTGGACATGAACCTGTTTCCCGGCGGCCACAACAACCTGAACCCGGAATTCCTGCCGCTGGCG
GTGCAGGCGGCGCAGAGCGCGGTGGACAAGGTATGCCCGGAGGCGCGCAGCATCCTGCTGATCCCGGAAAACCATACCCG
CAACACTTTCTACCTGCAGAACGTCGCCACGCTGGTGCACATTTTCGAGCAGGCCGGCCTCAAGGTGCGCCTGGGCTCGC
TGAGCCCCGAAATCACCGAGCCGACCGAGCTGGTGGCCGCCAACGGCGACAAGCTGACGCTGGAGCCGCTGGAGCGCCGC
GGCAACCGGCTGGTGCTGGCCGACGGCTTCAATCCCTGCGTGGTGCTGCTGAACAACGACCTGTCCGGCGGCATTCCGGA
CCTGTTGAAAAACCTGGAGCAGACGCTGCTGCCGCCGCTGCACGCCGGCTGGGCGGTACGCCGCAAGACGCATCATTTCG
AGGCCTACGACAAGGTCAGCACCGATTTCGCGCGGCTGATCTCGATCGACCCGTGGACCATCAATCCCTACTTCGCCAGC
TGCGGGGGCCTGGACTTCCACGCCCGCACCGGCGAGGAGCAGCTGGCCGAAACCGTGTCGCAGATGCTGGACAAGATCCG
CCGCAAGTACCGCGAGTACGGCATCGACAACGACCCCTTCGTCATCGTCAAGGCCGACGCCGGCACCTACGGCATGGGCG
TGATGAGCGTGAAGAGCCCGGACGAGGTGATCGGCCTGAACCGCAAGGCGCGCAACAAGATGTCGGTGGTCAAGGAAGGC
CTGGAAGTGTCGGAGGTGATCGTCCAGGAGGGCGTGTACACCTTCGAGACCGTCAACGAGGCGGTGGCCGAGCCGGTGGT
CTACATGATGGACCGCTACGTGATCGGCGGCTTCTACCGGGTCCACACCGGCCGCGGCATCGACGAGAACCTGAACGCCC
CGGGCATGCACTTCGTGCCGCTGTCGTTCGAGACGCCGTGCCTGCCGGACCGCAAGGGCCTGCCCGACTGCGCGCCCAAC
CGCTTCTACGCCTACGGCGTGATCTCGCGGCTGGCGCTGCTGGCGGCTTCGCTGGAGCTGGAAGCCACCGACCCGGACGC
TGAATAA

Upstream 100 bases:

>100_bases
CTTTCATCTGCCGCTCGACGCGCATTGAGCGCCGGCACTTGAATATGGCTGTCGCCACCCCCACCATTAGCGAATTCAAC
GTCCGTTCCAAGGGGATACC

Downstream 100 bases:

>100_bases
CCCCGCTGGAGCACAGACATGCGCATCCTGTTCATTGCCGACCCGCTGGAACACTTCAAGATTTACAAGGACACCACCTT
CGCGATGATGGAGGAGGCGC

Product: glutamate-cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Glutamate-Cysteine Ligase GshA; Glutamate-Cysteine Ligase-Related Protein; Glutathione Synthetase; GshA Protein

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MPPVPHLATALTGPLYELESRILAAQPQIEHWFRSQWHAHATPFYGSVDLRNSGFKLAPVDMNLFPGGHNNLNPEFLPLA
VQAAQSAVDKVCPEARSILLIPENHTRNTFYLQNVATLVHIFEQAGLKVRLGSLSPEITEPTELVAANGDKLTLEPLERR
GNRLVLADGFNPCVVLLNNDLSGGIPDLLKNLEQTLLPPLHAGWAVRRKTHHFEAYDKVSTDFARLISIDPWTINPYFAS
CGGLDFHARTGEEQLAETVSQMLDKIRRKYREYGIDNDPFVIVKADAGTYGMGVMSVKSPDEVIGLNRKARNKMSVVKEG
LEVSEVIVQEGVYTFETVNEAVAEPVVYMMDRYVIGGFYRVHTGRGIDENLNAPGMHFVPLSFETPCLPDRKGLPDCAPN
RFYAYGVISRLALLAASLELEATDPDAE

Sequences:

>Translated_428_residues
MPPVPHLATALTGPLYELESRILAAQPQIEHWFRSQWHAHATPFYGSVDLRNSGFKLAPVDMNLFPGGHNNLNPEFLPLA
VQAAQSAVDKVCPEARSILLIPENHTRNTFYLQNVATLVHIFEQAGLKVRLGSLSPEITEPTELVAANGDKLTLEPLERR
GNRLVLADGFNPCVVLLNNDLSGGIPDLLKNLEQTLLPPLHAGWAVRRKTHHFEAYDKVSTDFARLISIDPWTINPYFAS
CGGLDFHARTGEEQLAETVSQMLDKIRRKYREYGIDNDPFVIVKADAGTYGMGVMSVKSPDEVIGLNRKARNKMSVVKEG
LEVSEVIVQEGVYTFETVNEAVAEPVVYMMDRYVIGGFYRVHTGRGIDENLNAPGMHFVPLSFETPCLPDRKGLPDCAPN
RFYAYGVISRLALLAASLELEATDPDAE
>Mature_427_residues
PPVPHLATALTGPLYELESRILAAQPQIEHWFRSQWHAHATPFYGSVDLRNSGFKLAPVDMNLFPGGHNNLNPEFLPLAV
QAAQSAVDKVCPEARSILLIPENHTRNTFYLQNVATLVHIFEQAGLKVRLGSLSPEITEPTELVAANGDKLTLEPLERRG
NRLVLADGFNPCVVLLNNDLSGGIPDLLKNLEQTLLPPLHAGWAVRRKTHHFEAYDKVSTDFARLISIDPWTINPYFASC
GGLDFHARTGEEQLAETVSQMLDKIRRKYREYGIDNDPFVIVKADAGTYGMGVMSVKSPDEVIGLNRKARNKMSVVKEGL
EVSEVIVQEGVYTFETVNEAVAEPVVYMMDRYVIGGFYRVHTGRGIDENLNAPGMHFVPLSFETPCLPDRKGLPDCAPNR
FYAYGVISRLALLAASLELEATDPDAE

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 47457; Mature: 47325

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPVPHLATALTGPLYELESRILAAQPQIEHWFRSQWHAHATPFYGSVDLRNSGFKLAPV
CCCCCCHHHHHCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEECCCCCEEEEE
DMNLFPGGHNNLNPEFLPLAVQAAQSAVDKVCPEARSILLIPENHTRNTFYLQNVATLVH
CEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEHHHHHHHHH
IFEQAGLKVRLGSLSPEITEPTELVAANGDKLTLEPLERRGNRLVLADGFNPCVVLLNND
HHHHCCCEEEECCCCCCCCCCHHEEECCCCEEEECHHHHCCCEEEEECCCCCEEEEEECC
LSGGIPDLLKNLEQTLLPPLHAGWAVRRKTHHFEAYDKVSTDFARLISIDPWTINPYFAS
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCEECCCHHHC
CGGLDFHARTGEEQLAETVSQMLDKIRRKYREYGIDNDPFVIVKADAGTYGMGVMSVKSP
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCC
DEVIGLNRKARNKMSVVKEGLEVSEVIVQEGVYTFETVNEAVAEPVVYMMDRYVIGGFYR
HHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEE
VHTGRGIDENLNAPGMHFVPLSFETPCLPDRKGLPDCAPNRFYAYGVISRLALLAASLEL
EECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCEE
EATDPDAE
ECCCCCCC
>Mature Secondary Structure 
PPVPHLATALTGPLYELESRILAAQPQIEHWFRSQWHAHATPFYGSVDLRNSGFKLAPV
CCCCCHHHHHCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEECCCCCEEEEE
DMNLFPGGHNNLNPEFLPLAVQAAQSAVDKVCPEARSILLIPENHTRNTFYLQNVATLVH
CEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEHHHHHHHHH
IFEQAGLKVRLGSLSPEITEPTELVAANGDKLTLEPLERRGNRLVLADGFNPCVVLLNND
HHHHCCCEEEECCCCCCCCCCHHEEECCCCEEEECHHHHCCCEEEEECCCCCEEEEEECC
LSGGIPDLLKNLEQTLLPPLHAGWAVRRKTHHFEAYDKVSTDFARLISIDPWTINPYFAS
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCEECCCHHHC
CGGLDFHARTGEEQLAETVSQMLDKIRRKYREYGIDNDPFVIVKADAGTYGMGVMSVKSP
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCC
DEVIGLNRKARNKMSVVKEGLEVSEVIVQEGVYTFETVNEAVAEPVVYMMDRYVIGGFYR
HHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEE
VHTGRGIDENLNAPGMHFVPLSFETPCLPDRKGLPDCAPNRFYAYGVISRLALLAASLEL
EECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCEE
EATDPDAE
ECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA