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The map label for this gene is ribE [H]

Identifier: 34499672

GI number: 34499672

Start: 4551926

End: 4552636

Strand: Direct

Name: ribE [H]

Synonym: CV_4217

Alternate gene names: 34499672

Gene position: 4551926-4552636 (Clockwise)

Preceding gene: 34499671

Following gene: 34499673

Centisome position: 95.81

GC content: 72.57

Gene sequence:

>711_bases
ATGTTCACCGGCATCACCCAGGCCGTCGGCCGCATCGCCGCCATCTCCGAAGGCGAAGCCAGCCGCCGCGTCGCCATCGA
CTTCCCGGACGGCTTCTGCGCCGGCCTCGCCCAAGGCTGCAGCGTTTCCATCGACGGCGCCTGCCTGACCGTGGCCGCGC
CGCCGCGGGGCGAGCGCGCCGAGTTCGACCTGATCCTGCCGACGCTGGTCACCAGCACGCTGGCCGCGTGCCGCGTCGGC
GACGCGGTCAACGCCGAGCGCGCGCTGCGCGACGGCGCCGAGATCGGCGGCCACGCGCTGTCCGGCCACGTCGACTATCA
AGCCGTGGTCGAGCAGCGCAAGGAATGCGGCGACAACCTGTGCCTGCGGCTGTCGGTGCCGGCCGGCGCGCTGCGCTACC
TGTTCGCCAAGGGCTACGCGGCGCTGGACGGCGTCAGCCTGACCATCGCCGACATCGACAAGCAGGCCGGCTGGATCGAA
GTGTGGCTGATCCCGGAAACCCGCCGCGCCACCTCGCTCGGCGCCAAGCAACCCGGCTGCCGGCTGAACCTGGAAATCGA
GCGCGACACCCAGGTGCTGGTCGACACCATGCGCGAAGCGCTGGAGGACAAGCTGGCGGCCTTGATGCCGCTGCTCGCGC
CCAGGCTGGACGAACTGGCACCCGCGCTGTTGCCGGCGGCCCGCCGCCAGCCGGCGCTGGCCGCGCGCTGA

Upstream 100 bases:

>100_bases
AGCAAGTGCAGGATTACGCCGAGCGCCACGATCTGCCGCAGCTGTCGGTCGCCGAGCTGGCCGAATGGCTGCAGCGCGAA
CCGGCTTGAGGAGGCGCGAC

Downstream 100 bases:

>100_bases
CCGCAGGCGGCGGCGAACCAATCGGCCTCCCGCCTGTCTGCTGAACAGGGGCGCATCCGCGCCCCTGTTCCACTTTCATG
ACGCCATGCTCCGCTTCATT

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MFTGITQAVGRIAAISEGEASRRVAIDFPDGFCAGLAQGCSVSIDGACLTVAAPPRGERAEFDLILPTLVTSTLAACRVG
DAVNAERALRDGAEIGGHALSGHVDYQAVVEQRKECGDNLCLRLSVPAGALRYLFAKGYAALDGVSLTIADIDKQAGWIE
VWLIPETRRATSLGAKQPGCRLNLEIERDTQVLVDTMREALEDKLAALMPLLAPRLDELAPALLPAARRQPALAAR

Sequences:

>Translated_236_residues
MFTGITQAVGRIAAISEGEASRRVAIDFPDGFCAGLAQGCSVSIDGACLTVAAPPRGERAEFDLILPTLVTSTLAACRVG
DAVNAERALRDGAEIGGHALSGHVDYQAVVEQRKECGDNLCLRLSVPAGALRYLFAKGYAALDGVSLTIADIDKQAGWIE
VWLIPETRRATSLGAKQPGCRLNLEIERDTQVLVDTMREALEDKLAALMPLLAPRLDELAPALLPAARRQPALAAR
>Mature_236_residues
MFTGITQAVGRIAAISEGEASRRVAIDFPDGFCAGLAQGCSVSIDGACLTVAAPPRGERAEFDLILPTLVTSTLAACRVG
DAVNAERALRDGAEIGGHALSGHVDYQAVVEQRKECGDNLCLRLSVPAGALRYLFAKGYAALDGVSLTIADIDKQAGWIE
VWLIPETRRATSLGAKQPGCRLNLEIERDTQVLVDTMREALEDKLAALMPLLAPRLDELAPALLPAARRQPALAAR

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=202, Percent_Identity=38.6138613861386, Blast_Score=135, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6319733, Length=232, Percent_Identity=25, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 25015; Mature: 25015

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGITQAVGRIAAISEGEASRRVAIDFPDGFCAGLAQGCSVSIDGACLTVAAPPRGERA
CCCCHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHCCCCEEECCEEEEEECCCCCCCC
EFDLILPTLVTSTLAACRVGDAVNAERALRDGAEIGGHALSGHVDYQAVVEQRKECGDNL
CEEEEHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHCCEEECCCCHHHHHHHHHHHCCCCE
CLRLSVPAGALRYLFAKGYAALDGVSLTIADIDKQAGWIEVWLIPETRRATSLGAKQPGC
EEEEECCHHHHHHHHHCCCHHHCCCEEEEEECCCCCCEEEEEEECCCHHHHHCCCCCCCC
RLNLEIERDTQVLVDTMREALEDKLAALMPLLAPRLDELAPALLPAARRQPALAAR
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MFTGITQAVGRIAAISEGEASRRVAIDFPDGFCAGLAQGCSVSIDGACLTVAAPPRGERA
CCCCHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHCCCCEEECCEEEEEECCCCCCCC
EFDLILPTLVTSTLAACRVGDAVNAERALRDGAEIGGHALSGHVDYQAVVEQRKECGDNL
CEEEEHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHCCEEECCCCHHHHHHHHHHHCCCCE
CLRLSVPAGALRYLFAKGYAALDGVSLTIADIDKQAGWIEVWLIPETRRATSLGAKQPGC
EEEEECCHHHHHHHHHCCCHHHCCCEEEEEECCCCCCEEEEEEECCCHHHHHCCCCCCCC
RLNLEIERDTQVLVDTMREALEDKLAALMPLLAPRLDELAPALLPAARRQPALAAR
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9022701; 9023191; 9097039; 9278503; 11377200 [H]