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The map label for this gene is ruvB

Identifier: 34499670

GI number: 34499670

Start: 4549877

End: 4550914

Strand: Reverse

Name: ruvB

Synonym: CV_4215

Alternate gene names: 34499670

Gene position: 4550914-4549877 (Counterclockwise)

Preceding gene: 34499677

Following gene: 34499669

Centisome position: 95.79

GC content: 68.79

Gene sequence:

>1038_bases
ATGATAGAAACCGACAAGCTGTTCGGCGCCGCGCCGGAGCGCCGCATCGTCACCCCGCAACGCGCGTCCGAGCAGGAAGA
GGCGCTGGAGCGGGCGCTGCGGCCCAAGCTGCTGGACGAGTACGTGGGCCAGAAGAAGGCGCGCGAGCAGCTGGAAATCT
TCATCGAGGCGGCGAAGAAGCGCGGCGAGGCGCTGGACCACGTGCTGCTGTTCGGCCCGCCGGGCCTGGGCAAGACCACG
CTGGCCCACATCGTCGCGCGCGAGATGGGCGTCAACCTGCGCCAGACGTCTGGCCCGGTGCTCGAGCGCGCCGGCGACCT
GGCGGCGCTGTTGACCAATCTGGAGCCGCACGACGTGCTGTTCATCGACGAGATCCACCGCCTGTCACCGGTGGTGGAGG
AAATCCTCTACCCGGCGCTGGAGGATTACCAGATCGACATCATGATAGGCGAGGGGCCGGCGGCGCGGTCGGTGAAGATC
GACCTGCCGCCGTTCACGCTGGTGGGCGCCACCACCCGCGCCGGCATGCTGACCAATCCGCTGCGCGACCGCTTCGGCAT
CGTCGCCCGGCTGGAGTTCTACAACGCCGAGGAGCTGACCCGCATCGTCAGCCGTTCGGCCGGCCTCCTGAATGTGCAGT
TGTCGGACGACGGCGCCTTCGAGGTGGCCAAGCGCTCGCGCGGCACGCCGCGCATCGCCAACCGCCTGCTGCGCCGGGTG
CGCGACTACGCCGAGGTGAAATCCGACGGCGTGGTGACGATGGCGGTGGCCGACGCCGCGCTGGCGATGCTGGACGTCGA
TCCGGCCGGCCTCGATGTGATGGACCGCAAGCTGCTGCAGGCTATTCTGGAGAAGTTTTCCGGCGGGCCGGTGGGCCTGG
ACAACGTCGCCGCGGCGATCGGCGAGTCCACCGACACCATCGAGGACGTGATCGAGCCCTTCCTGATCCAGCAGGGCTAT
CTGCAGCGCACGCCGCGCGGCCGGATGGCCACCGCGCAGGCCTATCTGCATTTCGGCCTGCCGGTGAAGGACGCCTGA

Upstream 100 bases:

>100_bases
CCCGAAGACGTAAGCTTGCGGCCGCGTGTCGCGCGGCCAGCGGCGAGTATAGCGTGGTGTTAGAATGTCGGCAGCAAGAT
TCATCTTTCAAGAGTCCCCG

Downstream 100 bases:

>100_bases
GCACCCGCCATATGATTTGTTGAACGGCCCGGCGGGGCCGTTTTCGCGCCCGGCATGCGGCGGAAGGCGGTCATGCTTGT
CCGCGCGCAAAGGCTGGTCG

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MIETDKLFGAAPERRIVTPQRASEQEEALERALRPKLLDEYVGQKKAREQLEIFIEAAKKRGEALDHVLLFGPPGLGKTT
LAHIVAREMGVNLRQTSGPVLERAGDLAALLTNLEPHDVLFIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARSVKI
DLPPFTLVGATTRAGMLTNPLRDRFGIVARLEFYNAEELTRIVSRSAGLLNVQLSDDGAFEVAKRSRGTPRIANRLLRRV
RDYAEVKSDGVVTMAVADAALAMLDVDPAGLDVMDRKLLQAILEKFSGGPVGLDNVAAAIGESTDTIEDVIEPFLIQQGY
LQRTPRGRMATAQAYLHFGLPVKDA

Sequences:

>Translated_345_residues
MIETDKLFGAAPERRIVTPQRASEQEEALERALRPKLLDEYVGQKKAREQLEIFIEAAKKRGEALDHVLLFGPPGLGKTT
LAHIVAREMGVNLRQTSGPVLERAGDLAALLTNLEPHDVLFIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARSVKI
DLPPFTLVGATTRAGMLTNPLRDRFGIVARLEFYNAEELTRIVSRSAGLLNVQLSDDGAFEVAKRSRGTPRIANRLLRRV
RDYAEVKSDGVVTMAVADAALAMLDVDPAGLDVMDRKLLQAILEKFSGGPVGLDNVAAAIGESTDTIEDVIEPFLIQQGY
LQRTPRGRMATAQAYLHFGLPVKDA
>Mature_345_residues
MIETDKLFGAAPERRIVTPQRASEQEEALERALRPKLLDEYVGQKKAREQLEIFIEAAKKRGEALDHVLLFGPPGLGKTT
LAHIVAREMGVNLRQTSGPVLERAGDLAALLTNLEPHDVLFIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARSVKI
DLPPFTLVGATTRAGMLTNPLRDRFGIVARLEFYNAEELTRIVSRSAGLLNVQLSDDGAFEVAKRSRGTPRIANRLLRRV
RDYAEVKSDGVVTMAVADAALAMLDVDPAGLDVMDRKLLQAILEKFSGGPVGLDNVAAAIGESTDTIEDVIEPFLIQQGY
LQRTPRGRMATAQAYLHFGLPVKDA

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=340, Percent_Identity=71.4705882352941, Blast_Score=496, Evalue=1e-141,
Organism=Escherichia coli, GI1787119, Length=234, Percent_Identity=28.2051282051282, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_CHRVO (Q7NQC5)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903885.1
- ProteinModelPortal:   Q7NQC5
- SMR:   Q7NQC5
- GeneID:   2549625
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_4215
- NMPDR:   fig|243365.1.peg.4215
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- PhylomeDB:   Q7NQC5
- ProtClustDB:   PRK00080
- BioCyc:   CVIO243365:CV_4215-MONOMER
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 37864; Mature: 37864

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIETDKLFGAAPERRIVTPQRASEQEEALERALRPKLLDEYVGQKKAREQLEIFIEAAKK
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHCHHHHHHHHHHHHHHHHH
RGEALDHVLLFGPPGLGKTTLAHIVAREMGVNLRQTSGPVLERAGDLAALLTNLEPHDVL
HHHHHCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEE
FIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARSVKIDLPPFTLVGATTRAGMLTNP
EHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEECCCEEEECCCHHCCHHHHH
LRDRFGIVARLEFYNAEELTRIVSRSAGLLNVQLSDDGAFEVAKRSRGTPRIANRLLRRV
HHHHHCEEEEEECCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHH
RDYAEVKSDGVVTMAVADAALAMLDVDPAGLDVMDRKLLQAILEKFSGGPVGLDNVAAAI
HHHHHHCCCCEEEEHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
GESTDTIEDVIEPFLIQQGYLQRTPRGRMATAQAYLHFGLPVKDA
CCCHHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHEECCCCCCC
>Mature Secondary Structure
MIETDKLFGAAPERRIVTPQRASEQEEALERALRPKLLDEYVGQKKAREQLEIFIEAAKK
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHCHHHHHHHHHHHHHHHHH
RGEALDHVLLFGPPGLGKTTLAHIVAREMGVNLRQTSGPVLERAGDLAALLTNLEPHDVL
HHHHHCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEE
FIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARSVKIDLPPFTLVGATTRAGMLTNP
EHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEECCCEEEECCCHHCCHHHHH
LRDRFGIVARLEFYNAEELTRIVSRSAGLLNVQLSDDGAFEVAKRSRGTPRIANRLLRRV
HHHHHCEEEEEECCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHH
RDYAEVKSDGVVTMAVADAALAMLDVDPAGLDVMDRKLLQAILEKFSGGPVGLDNVAAAI
HHHHHHCCCCEEEEHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
GESTDTIEDVIEPFLIQQGYLQRTPRGRMATAQAYLHFGLPVKDA
CCCHHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782