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The map label for this gene is tufB

Identifier: 34499655

GI number: 34499655

Start: 4532139

End: 4533329

Strand: Reverse

Name: tufB

Synonym: CV_4200

Alternate gene names: 34499655

Gene position: 4533329-4532139 (Counterclockwise)

Preceding gene: 34499656

Following gene: 34499654

Centisome position: 95.42

GC content: 61.46

Gene sequence:

>1191_bases
ATGGCTAAGGAAAAGTTCGAGCGGACCAAACCGCACGTAAACGTCGGCACCATCGGTCACGTTGACCATGGTAAAACCAC
TCTGACCGCTGCTATCACCACCATTCTGTCGAAGAAGTTCGGTGGCGAAGCCAAAGACTACTCCCAGATCGACAGCGCGC
CGGAAGAGAAGGCTCGTGGTATCACCATCAATACCGCGCACGTTGAATACGAAACCGAGACCCGCCACTACGCTCACGTA
GACTGCCCGGGCCACGCCGACTACGTTAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTCTGCTC
GGCCGCTGACGGTCCGATGCCGCAAACCCGCGAACACATCCTGCTGTCCCGCCAGGTTGGCGTGCCGTACATCATCGTCT
ACCTGAACAAGGCTGACCTGGTTGACGACGCCGAGCTGCTGGAACTGGTGGAAATGGAAGTGCGCGATCTGCTGTCTTCC
TACGACTTCCCGGGCGACGACACCCCGATCGTGACCGGCTCCGCTCGTCTGGCGCTGGAAGGCGACCAGTCCGAAATGGG
CGAACCGTCGATCTTCCGTCTGGCTGACGCGCTGGATTCCTACATCCCGACTCCGGAGCGCGCCATCGACAAGCCGTTCC
TGCTGCCGATCGAAGACGTGTTCTCGATCTCCGGCCGCGGCACCGTGGTGACCGGTCGCGTAGAGCGCGGCATCGTGAAA
GTGGGCGAAGAACTGGAAATCGTGGGTCTGAAAGACACCGTGAAGACCACCTGCACCGGCGTGGAAATGTTCCGCAAGCT
GCTGGACCAGGGTCAGGCCGGCGACAACGTGGGCGTGCTGCTGCGCGGCACCAAGCGTGAAGACGTGGAGCGCGGCCAGG
TTCTGGCCAAGCCGGGCACCATCACCCCGCACACCAAGTTCGAAGCTTCGGTGTACGTTCTGTCCAAGGACGAAGGTGGC
CGTCACACCCCGTTCTTCGCGAACTACCGTCCGCAGTTCTACTTCCGCACCACCGACGTGACCGGCGCGATCTCGCTGGC
CGAAGGCGTGGAAATGGTAATGCCGGGCGACAACGTGGAAATCAAGGTTGAGCTGATCGCCCCGATCGCCATGGAAGAAG
GTCTGCGCTTCGCCATCCGCGAAGGCGGCCGTACCGTCGGCGCCGGCGTGGTTGCCAAGATCATCGCTTAA

Upstream 100 bases:

>100_bases
GGGTAGAGCACTCCCTTGGTAAGGGAGAGGTCGGCAGTTCAATTCTGCCCATGGGCACCATAGTTCAATTTTAGCTTGTT
TCGTATTCAGGAAATTTGCC

Downstream 100 bases:

>100_bases
TAAGGTTTAAAGGCCAGTAGCTCAATTGGTAGAGTATCGGTCTCCAAAACCGAGGGTTGGGGGTTCGAGACCCTCCTGGC
CTGCCAATTAAGACCAGCCG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTILSKKFGGEAKDYSQIDSAPEEKARGITINTAHVEYETETRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPYIIVYLNKADLVDDAELLELVEMEVRDLLSS
YDFPGDDTPIVTGSARLALEGDQSEMGEPSIFRLADALDSYIPTPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIVK
VGEELEIVGLKDTVKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVLAKPGTITPHTKFEASVYVLSKDEGG
RHTPFFANYRPQFYFRTTDVTGAISLAEGVEMVMPGDNVEIKVELIAPIAMEEGLRFAIREGGRTVGAGVVAKIIA

Sequences:

>Translated_396_residues
MAKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTILSKKFGGEAKDYSQIDSAPEEKARGITINTAHVEYETETRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPYIIVYLNKADLVDDAELLELVEMEVRDLLSS
YDFPGDDTPIVTGSARLALEGDQSEMGEPSIFRLADALDSYIPTPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIVK
VGEELEIVGLKDTVKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVLAKPGTITPHTKFEASVYVLSKDEGG
RHTPFFANYRPQFYFRTTDVTGAISLAEGVEMVMPGDNVEIKVELIAPIAMEEGLRFAIREGGRTVGAGVVAKIIA
>Mature_395_residues
AKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTILSKKFGGEAKDYSQIDSAPEEKARGITINTAHVEYETETRHYAHVD
CPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPYIIVYLNKADLVDDAELLELVEMEVRDLLSSY
DFPGDDTPIVTGSARLALEGDQSEMGEPSIFRLADALDSYIPTPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIVKV
GEELEIVGLKDTVKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVLAKPGTITPHTKFEASVYVLSKDEGGR
HTPFFANYRPQFYFRTTDVTGAISLAEGVEMVMPGDNVEIKVELIAPIAMEEGLRFAIREGGRTVGAGVVAKIIA

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=57.2151898734177, Blast_Score=490, Evalue=1e-139,
Organism=Homo sapiens, GI223555963, Length=435, Percent_Identity=30.8045977011494, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI5729864, Length=435, Percent_Identity=30.8045977011494, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI4503475, Length=451, Percent_Identity=28.159645232816, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI4503471, Length=452, Percent_Identity=29.8672566371681, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI194097354, Length=338, Percent_Identity=30.7692307692308, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI194018520, Length=338, Percent_Identity=30.7692307692308, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI194018522, Length=438, Percent_Identity=27.8538812785388, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI46094014, Length=438, Percent_Identity=27.8538812785388, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI54607086, Length=322, Percent_Identity=27.9503105590062, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI4503507, Length=363, Percent_Identity=28.099173553719, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=32.5925925925926, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=82.0707070707071, Blast_Score=642, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=82.0707070707071, Blast_Score=640, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=384, Percent_Identity=30.46875, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI48994988, Length=275, Percent_Identity=31.6363636363636, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1789108, Length=335, Percent_Identity=30.1492537313433, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=53.8847117794486, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=43.9276485788114, Blast_Score=330, Evalue=9e-91,
Organism=Caenorhabditis elegans, GI17552884, Length=450, Percent_Identity=29.5555555555556, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17569207, Length=450, Percent_Identity=29.5555555555556, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI32566303, Length=430, Percent_Identity=28.8372093023256, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI115532067, Length=432, Percent_Identity=28.4722222222222, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=432, Percent_Identity=28.7037037037037, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=452, Percent_Identity=26.5486725663717, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17506081, Length=349, Percent_Identity=30.3724928366762, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17509919, Length=445, Percent_Identity=26.5168539325843, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI32566301, Length=154, Percent_Identity=36.3636363636364, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=233, Percent_Identity=24.8927038626609, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=395, Percent_Identity=66.3291139240506, Blast_Score=544, Evalue=1e-156,
Organism=Saccharomyces cerevisiae, GI6325337, Length=441, Percent_Identity=29.9319727891156, Blast_Score=156, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6319594, Length=441, Percent_Identity=29.9319727891156, Blast_Score=156, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6320377, Length=339, Percent_Identity=30.6784660766962, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322937, Length=351, Percent_Identity=25.6410256410256, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=269, Percent_Identity=27.8810408921933, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6323320, Length=256, Percent_Identity=26.953125, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=57.5063613231552, Blast_Score=486, Evalue=1e-137,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=57.5063613231552, Blast_Score=486, Evalue=1e-137,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=53.1088082901554, Blast_Score=422, Evalue=1e-118,
Organism=Drosophila melanogaster, GI45553807, Length=455, Percent_Identity=30.989010989011, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI45553816, Length=455, Percent_Identity=30.989010989011, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24651721, Length=455, Percent_Identity=30.989010989011, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI17864154, Length=455, Percent_Identity=30.989010989011, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24652838, Length=452, Percent_Identity=29.8672566371681, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI17137572, Length=452, Percent_Identity=29.8672566371681, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI45550900, Length=448, Percent_Identity=28.5714285714286, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI17137380, Length=346, Percent_Identity=30.3468208092486, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=29.8360655737705, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24647046, Length=361, Percent_Identity=27.9778393351801, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24647048, Length=361, Percent_Identity=27.9778393351801, Blast_Score=92, Evalue=8e-19,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_CHRVO (Q7M7F1)

Other databases:

- EMBL:   AE016825
- EMBL:   AE016825
- RefSeq:   NP_903858.1
- RefSeq:   NP_903870.1
- HSSP:   P0A6N1
- ProteinModelPortal:   Q7M7F1
- SMR:   Q7M7F1
- GeneID:   2548977
- GeneID:   2549633
- GenomeReviews:   AE016825_GR
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_4188
- KEGG:   cvi:CV_4200
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- PhylomeDB:   Q7M7F1
- BioCyc:   CVIO243365:CV_4188-MONOMER
- BioCyc:   CVIO243365:CV_4200-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 43073; Mature: 42941

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTILSKKFGGEAKDYSQIDSAPEEKARG
CCCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHCC
ITINTAHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPYIIVYLNKADLVDDAELLELVEMEVRDLLSSYDFPGDDTPIVTGSARLALE
EEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEE
GDQSEMGEPSIFRLADALDSYIPTPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIVK
CCHHHCCCCHHHHHHHHHHHHCCCCHHHCCCCCEEEHHHHHCCCCCCEEEECHHHHCHHH
VGEELEIVGLKDTVKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVLAKPGT
CCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEECCCC
ITPHTKFEASVYVLSKDEGGRHTPFFANYRPQFYFRTTDVTGAISLAEGVEMVMPGDNVE
CCCCCCCEEEEEEEECCCCCCCCCEEECCCCEEEEEECCCCCEEEHHCCCEEEECCCCCE
IKVELIAPIAMEEGLRFAIREGGRTVGAGVVAKIIA
EEEEEEECCHHHCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTILSKKFGGEAKDYSQIDSAPEEKARG
CCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHCC
ITINTAHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPYIIVYLNKADLVDDAELLELVEMEVRDLLSSYDFPGDDTPIVTGSARLALE
EEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEE
GDQSEMGEPSIFRLADALDSYIPTPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIVK
CCHHHCCCCHHHHHHHHHHHHCCCCHHHCCCCCEEEHHHHHCCCCCCEEEECHHHHCHHH
VGEELEIVGLKDTVKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVLAKPGT
CCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEECCCC
ITPHTKFEASVYVLSKDEGGRHTPFFANYRPQFYFRTTDVTGAISLAEGVEMVMPGDNVE
CCCCCCCEEEEEEEECCCCCCCCCEEECCCCEEEEEECCCCCEEEHHCCCEEEECCCCCE
IKVELIAPIAMEEGLRFAIREGGRTVGAGVVAKIIA
EEEEEEECCHHHCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782