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The map label for this gene is lacA [C]

Identifier: 34499580

GI number: 34499580

Start: 4466246

End: 4466827

Strand: Reverse

Name: lacA [C]

Synonym: CV_4125

Alternate gene names: 34499580

Gene position: 4466827-4466246 (Counterclockwise)

Preceding gene: 34499581

Following gene: 34499579

Centisome position: 94.02

GC content: 65.98

Gene sequence:

>582_bases
ATGAGCCAGGTTCACATCCACCCCAGCGCCATCGTCGACGAAGGCGCCGCCCTGGGCGAAGGCACCCGCGTCTGGCACTG
GGTGCACATCTGCGGCGGCGCGAAGATAGGCAAGGGCTGCTCCTTCGGCCAGAACGTGTTCGTCGGCAACGACGTGCTGA
TCGGCGACAACGTCAAGGTGCAGAACAACGTGTCGATCTACGACGCGGTGACGCTGGAGGACGACGTGTTCTGCGGGCCG
TCCATGGTGTTCACCAACGTCAACAATCCGCGCAGCCACGTCAACCGCAAGAACGAATACCGCCGCACCGTGGTGAAGAA
GGGCGCGTCCATCGGCGCCAACGCCACCATCGTCTGCGGCCACACGGTGGGCGAGTACGCGTTCATCGGCGCCGGCGCGG
TGGTGACCCGCGACGTGCCGGCCTACGCGCTGATGGTGGGCACGCCGGCCAAGCGCATCGGCTGGATGTGCTCCTGCGGC
GAGCGTCTGTCCAACGACATCGGCACCCACTCCTGCCCGGTGTGCGCCATTCATTACGAGGTCGGCGGCAACCACTGCAC
GCCGATCGGCAACGGCGGATAA

Upstream 100 bases:

>100_bases
GCTATGAGGACTTGATGAGTCGTCAGGAAAAGGCGATGGTTCGTTCGGAGCAGCAATTGACTCGCCTGGACAAGCTTTTG
GAAAAATGGGAGCGGCAAAA

Downstream 100 bases:

>100_bases
GCGTCTCAGGCGCGCAGGCCGGCCGCGCGATCGGCGCGGCCGGCGCCGGGACGCTTGTTCAGCGTGCATTTTTCAAAATA
CTGATTTGGGGTTGAGCCTC

Product: acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: Hexapaptide Repeat-Containing Transferase; Transferase Hexapeptide Repeat Containing Protein; Hexapeptide Transferase Family Protein; Acetyl/Acyl Transferase Related Protein; WxcM-Like Protein; WxcM Domain-Containing Protein; WbbJ Protein; Isoleucine Patch Superfamily Acetyltransferase; WblC Protein; Hexapeptide Repeat-Containing Transferase; Acetyltransferases; Maltose O-Acetyltransferase; Acetyl / Acyl Transferase Related Protein; Acyltransferase; WxcM-Like; Acetyltransferase Protein; Transferase Hexapeptide Repeat; Acetyl Transferase; CysE Serine O-Acetyltransferase; Glucose-Fructose Oxidoreductase; Oxidoreductase Domain Protein; Acetyltransferase WbpD; UDP-3-O-(3-Hydroxymyristoyl)-Like Protein; Transferase Hexapeptide Repeat-Containing Protein; Carbonic Anhydrase/Acetyltransferase; Hexapeptide Transferase Family Protein Acetyltransferase; NDP-Sugar Acetyltransferase; Serine Acetyltransferase-Like Protein; Acetyl Transferase/Isomerase; Bifunctional Acetyl Transferase/Isomerase; N-Acetylglucosamine-1-Phosphate Uridyltransferase; Hexapeptide Repeat-Containing Acetyltransferase; Transferase Protein; Hexapeptide Transferase; Carbohydrate Kinase Rmoresistant Glucokinase; Lipopolysaccharide Biosynthesis Protein; Bacterial Transferase Hexapeptide-Like Protein; Transferase Hexapeptide Family; Bacterial Transferase Hexapeptide Repeat Protein; 3-Hydroxymyristoyl; UDP-3-O-[3-Hydroxymyristoyl] Glucosamine N-Acyltransferase; Acetyl/Acyl Transferase-Like Protein; Acetyltransferase CysE/LacA/LpxA/NodL Family; Carbonic Anhydrases/Acetyltransferases

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MSQVHIHPSAIVDEGAALGEGTRVWHWVHICGGAKIGKGCSFGQNVFVGNDVLIGDNVKVQNNVSIYDAVTLEDDVFCGP
SMVFTNVNNPRSHVNRKNEYRRTVVKKGASIGANATIVCGHTVGEYAFIGAGAVVTRDVPAYALMVGTPAKRIGWMCSCG
ERLSNDIGTHSCPVCAIHYEVGGNHCTPIGNGG

Sequences:

>Translated_193_residues
MSQVHIHPSAIVDEGAALGEGTRVWHWVHICGGAKIGKGCSFGQNVFVGNDVLIGDNVKVQNNVSIYDAVTLEDDVFCGP
SMVFTNVNNPRSHVNRKNEYRRTVVKKGASIGANATIVCGHTVGEYAFIGAGAVVTRDVPAYALMVGTPAKRIGWMCSCG
ERLSNDIGTHSCPVCAIHYEVGGNHCTPIGNGG
>Mature_192_residues
SQVHIHPSAIVDEGAALGEGTRVWHWVHICGGAKIGKGCSFGQNVFVGNDVLIGDNVKVQNNVSIYDAVTLEDDVFCGPS
MVFTNVNNPRSHVNRKNEYRRTVVKKGASIGANATIVCGHTVGEYAFIGAGAVVTRDVPAYALMVGTPAKRIGWMCSCGE
RLSNDIGTHSCPVCAIHYEVGGNHCTPIGNGG

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 20395; Mature: 20264

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
4.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVHIHPSAIVDEGAALGEGTRVWHWVHICGGAKIGKGCSFGQNVFVGNDVLIGDNVKV
CCEEEECCHHHHCCCCCCCCCCEEEEEEEEECCCEECCCCCCCCEEEECCEEEECCCEEE
QNNVSIYDAVTLEDDVFCGPSMVFTNVNNPRSHVNRKNEYRRTVVKKGASIGANATIVCG
CCCCEEEEEEEECCCCCCCCCEEEEECCCHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEC
HTVGEYAFIGAGAVVTRDVPAYALMVGTPAKRIGWMCSCGERLSNDIGTHSCPVCAIHYE
CCCCCEEEECCCEEEEECCCEEEEEECCCHHHHCEEEHHHHHHHHCCCCCCCCEEEEEEE
VGGNHCTPIGNGG
CCCCCCCCCCCCC
>Mature Secondary Structure 
SQVHIHPSAIVDEGAALGEGTRVWHWVHICGGAKIGKGCSFGQNVFVGNDVLIGDNVKV
CEEEECCHHHHCCCCCCCCCCEEEEEEEEECCCEECCCCCCCCEEEECCEEEECCCEEE
QNNVSIYDAVTLEDDVFCGPSMVFTNVNNPRSHVNRKNEYRRTVVKKGASIGANATIVCG
CCCCEEEEEEEECCCCCCCCCEEEEECCCHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEC
HTVGEYAFIGAGAVVTRDVPAYALMVGTPAKRIGWMCSCGERLSNDIGTHSCPVCAIHYE
CCCCCEEEECCCEEEEECCCEEEEEECCCHHHHCEEEHHHHHHHHCCCCCCCCEEEEEEE
VGGNHCTPIGNGG
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA