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The map label for this gene is wbjE [H]

Identifier: 34499574

GI number: 34499574

Start: 4459659

End: 4460888

Strand: Reverse

Name: wbjE [H]

Synonym: CV_4119

Alternate gene names: 34499574

Gene position: 4460888-4459659 (Counterclockwise)

Preceding gene: 34499575

Following gene: 34499573

Centisome position: 93.89

GC content: 65.53

Gene sequence:

>1230_bases
ATGAGAATACTGTATATCAACCATTACGCCGGCAGCCCGCGCCACGGCATGGAATTCCGCCCCTACTACCTGGCGCGGGA
ATGGGTAAAAGCCGGGCATGAAGTGAACATGCTGGCGGCCGATGTGTCGCACCTGCGCCAGACCCATCCCCAGGTGGACG
GCAAGTACCGCGACGAGGACATCGACGGCATCCGCTACACCTGGTGCAAGACGCGGCCATACAGCGGCAACGGCGTCGGC
CGGGTGCTTAACATCTTCAGTTTCCTGGCCAAGGTGATGGGCCTGTCGCGCCGCTACCTGAAGGAATGGCAGCCGGACGT
GGTGATCGCCTCGTCCACCTATCCGCTGGATACCGTGCCGGCGGCGTGGATCGCCGGCAAGACCGGCGCCCGGCTGGTGT
ACGAGGTGCACGACCTGTGGCCGCTGACGCCGGTGGAAGTGGGCGGCATGTCGCCCAAGCACCCGTTCATCCGCTTGCTG
CAATGGGCGGAGGATTTCGGCTACAAGCGCGCCGACACCGTGGTGTCGATGCTGCCCTGCGCCAGGGACTACATGATGGC
GCACGGCATGGCGGCGGAAAAATACGCGGTCATCCCCAATGGCGTGGACGTCAGCGAATGGCAGGGCGAGACGCTGCCGC
TGTCGGCCGAGCACCGGATGCGGTTGGCGGAGCTGAAGTCCCATGGCCGCTTCATCGTCGGCTACGCCGGCGGCCATGGC
CTGGCCAACGCGCTGGATTATCTGTTGGAAGCCGCGGCCTTGATGCGCGACGCGCCGGTGACTTTCGTGCTGGTGGGCGA
CGGTCCGGACAAGCAGGCGCTGGTGTCGCGCGCCGCGGAACTGAAGCTGGACAACGTGATGTTCCTGCCGTCCATCGCCA
AGCGCAGCGTGCCCGCCTTCCTGGCCGAGTGCGACGCGCTGTACATCGGCTGGCGCAAGCTGCCGATCTACCGCTTCGGC
ATCAACCCGAACAAGCTGTTCGATTACATGATGGCGGGCAAGCCGGTGATCCACTCGGTGGAGGCCGGCAACGATATGGT
CAAGGATGCCGGCGCCGGCTTGAGCATAGGCGTGGAAGACCCGGCCGTCATCGCCGACGCGGTCAAGCGCATGGCGGTGC
TGCCCTTGGCCGAGCGCGAGACGATGGGCGCGGCCGGCAAGGCCTACGTACTGGCCAACCATGATTACGCGGTGCTGGCG
CGGCGCTTTCTGGCGGCGGTGAAGCTGTGA

Upstream 100 bases:

>100_bases
CCACACGGAAAGACATGGAAGCATCGCTGTTGAAGTCTGGACAGCTGCAGTTCAAGCCGCACTTGAGTTTTGTCCGTGTT
GTTCCGTGGCTAATTACAAG

Downstream 100 bases:

>100_bases
TTTGGGCGTAGGGCGGAAGCCCCCGTTTTTTGGGGGCGTTCCGCCACGAGGCGATCGCCCTGTCGATATCGTGAGGGTTT
TAGCGGCGGAACGCCCCTGT

Product: glycosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MRILYINHYAGSPRHGMEFRPYYLAREWVKAGHEVNMLAADVSHLRQTHPQVDGKYRDEDIDGIRYTWCKTRPYSGNGVG
RVLNIFSFLAKVMGLSRRYLKEWQPDVVIASSTYPLDTVPAAWIAGKTGARLVYEVHDLWPLTPVEVGGMSPKHPFIRLL
QWAEDFGYKRADTVVSMLPCARDYMMAHGMAAEKYAVIPNGVDVSEWQGETLPLSAEHRMRLAELKSHGRFIVGYAGGHG
LANALDYLLEAAALMRDAPVTFVLVGDGPDKQALVSRAAELKLDNVMFLPSIAKRSVPAFLAECDALYIGWRKLPIYRFG
INPNKLFDYMMAGKPVIHSVEAGNDMVKDAGAGLSIGVEDPAVIADAVKRMAVLPLAERETMGAAGKAYVLANHDYAVLA
RRFLAAVKL

Sequences:

>Translated_409_residues
MRILYINHYAGSPRHGMEFRPYYLAREWVKAGHEVNMLAADVSHLRQTHPQVDGKYRDEDIDGIRYTWCKTRPYSGNGVG
RVLNIFSFLAKVMGLSRRYLKEWQPDVVIASSTYPLDTVPAAWIAGKTGARLVYEVHDLWPLTPVEVGGMSPKHPFIRLL
QWAEDFGYKRADTVVSMLPCARDYMMAHGMAAEKYAVIPNGVDVSEWQGETLPLSAEHRMRLAELKSHGRFIVGYAGGHG
LANALDYLLEAAALMRDAPVTFVLVGDGPDKQALVSRAAELKLDNVMFLPSIAKRSVPAFLAECDALYIGWRKLPIYRFG
INPNKLFDYMMAGKPVIHSVEAGNDMVKDAGAGLSIGVEDPAVIADAVKRMAVLPLAERETMGAAGKAYVLANHDYAVLA
RRFLAAVKL
>Mature_409_residues
MRILYINHYAGSPRHGMEFRPYYLAREWVKAGHEVNMLAADVSHLRQTHPQVDGKYRDEDIDGIRYTWCKTRPYSGNGVG
RVLNIFSFLAKVMGLSRRYLKEWQPDVVIASSTYPLDTVPAAWIAGKTGARLVYEVHDLWPLTPVEVGGMSPKHPFIRLL
QWAEDFGYKRADTVVSMLPCARDYMMAHGMAAEKYAVIPNGVDVSEWQGETLPLSAEHRMRLAELKSHGRFIVGYAGGHG
LANALDYLLEAAALMRDAPVTFVLVGDGPDKQALVSRAAELKLDNVMFLPSIAKRSVPAFLAECDALYIGWRKLPIYRFG
INPNKLFDYMMAGKPVIHSVEAGNDMVKDAGAGLSIGVEDPAVIADAVKRMAVLPLAERETMGAAGKAYVLANHDYAVLA
RRFLAAVKL

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1788363, Length=394, Percent_Identity=23.0964467005076, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: NA

Molecular weight: Translated: 45362; Mature: 45362

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILYINHYAGSPRHGMEFRPYYLAREWVKAGHEVNMLAADVSHLRQTHPQVDGKYRDED
CEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCCCCCCCCC
IDGIRYTWCKTRPYSGNGVGRVLNIFSFLAKVMGLSRRYLKEWQPDVVIASSTYPLDTVP
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCC
AAWIAGKTGARLVYEVHDLWPLTPVEVGGMSPKHPFIRLLQWAEDFGYKRADTVVSMLPC
HHHHCCCCCCEEEEEEHHCCCCCCEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
ARDYMMAHGMAAEKYAVIPNGVDVSEWQGETLPLSAEHRMRLAELKSHGRFIVGYAGGHG
HHHHHHHCCCCCCCEEECCCCCCHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCH
LANALDYLLEAAALMRDAPVTFVLVGDGPDKQALVSRAAELKLDNVMFLPSIAKRSVPAF
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEECCHHHHHHHHHH
LAECDALYIGWRKLPIYRFGINPNKLFDYMMAGKPVIHSVEAGNDMVKDAGAGLSIGVED
HHHCCEEEEEEEECEEEEECCCHHHHHHHHHCCCCCEEEECCCCHHHHHCCCCCEECCCC
PAVIADAVKRMAVLPLAERETMGAAGKAYVLANHDYAVLARRFLAAVKL
HHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRILYINHYAGSPRHGMEFRPYYLAREWVKAGHEVNMLAADVSHLRQTHPQVDGKYRDED
CEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCCCCCCCCC
IDGIRYTWCKTRPYSGNGVGRVLNIFSFLAKVMGLSRRYLKEWQPDVVIASSTYPLDTVP
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCC
AAWIAGKTGARLVYEVHDLWPLTPVEVGGMSPKHPFIRLLQWAEDFGYKRADTVVSMLPC
HHHHCCCCCCEEEEEEHHCCCCCCEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
ARDYMMAHGMAAEKYAVIPNGVDVSEWQGETLPLSAEHRMRLAELKSHGRFIVGYAGGHG
HHHHHHHCCCCCCCEEECCCCCCHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCH
LANALDYLLEAAALMRDAPVTFVLVGDGPDKQALVSRAAELKLDNVMFLPSIAKRSVPAF
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEECCHHHHHHHHHH
LAECDALYIGWRKLPIYRFGINPNKLFDYMMAGKPVIHSVEAGNDMVKDAGAGLSIGVED
HHHCCEEEEEEEECEEEEECCCHHHHHHHHHCCCCCEEEECCCCHHHHHCCCCCEECCCC
PAVIADAVKRMAVLPLAERETMGAAGKAYVLANHDYAVLARRFLAAVKL
HHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10400585; 10828401; 12057956 [H]