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The map label for this gene is rep [H]

Identifier: 34499523

GI number: 34499523

Start: 4398913

End: 4400934

Strand: Direct

Name: rep [H]

Synonym: CV_4068

Alternate gene names: 34499523

Gene position: 4398913-4400934 (Clockwise)

Preceding gene: 34499518

Following gene: 34499533

Centisome position: 92.59

GC content: 63.5

Gene sequence:

>2022_bases
ATGTCCGCTCCCCTGCTCAATCCGCCGCAACGCGCGGCCATCCATTATCTCGACGGCCCCTTGCTGGTGCTGGCGGGCGC
CGGCTCGGGCAAGACCCGGGTGATCACCTACAAGATCGCCCACCTGGTGCGCGAGGGCGGCATCCCGGCCCGGCACATCG
CCGCCATCACCTTCACCAACAAGGCGGCGCGCGAAATGCTGGAGCGGGTCGGCAAGCTGATGAGCTCGGCGGAGATCCGC
GGCATCACCGTCTCCACCTTCCACTCGCTCGGCATGCACATCCTGCGCCAGGAAGCGCCCCATCTGGGCTACAAGACCCA
ATTCTCCATTCTTGACGCCTACGATGCCGGCAAGATCATCGCCGACATCCTCAACACCACTTCCAAGGATGAGATCCGCA
AGGTGCAGAGCCAGATCTCGCTGTGGAAGAACGATCTGAAAACGCCGGACGACATGCTGCTGGCGGCGCAGGATCAATGG
GAAAGCGTCTGCGCCAAGACCTACCTCGCCTATCAGGACACCCTGACCGCCTACCAGGCGATGGACTTCGACGATCTGAT
CCGGCTGCCGGTGCAGCTGTTCCGCGAACATCCGGAAGTGCTGCTCAAGTGGCAGGGCAAGCTGCGCTACCTGCTGATAG
ACGAATACCAGGACACCAACACCTGCCAGTACCAGCTGGTGAAGCTGCTGACCGGCGTGCGCGGCCTGTTCACCGCGGTG
GGCGACGACGACCAGTCCATCTACGCCTGGCGCGGCGCGAACATGGAAAACCTGCGGCTGCTGCAGCAGGACTTTCCGGC
ATTGAAGGTGATCAAGCTGGAGCAGAACTACCGCTCCACCGCCCGCATCCTGCGCGCGGCCAACAGCGTGATCTCCAACA
ACCCCAAGCTGTTCGAGAAACAGCTGTGGAGCGAGCTGGGCCTGGGCGAGCCGATACACGTGGTGCAATGCAAGGACGAG
GAGCACGAGGCCGAAACCGTGGTGCAGCGGCTGCTGGCGCACAAGTTCGAATGCCGGACGGAGTTCAAGGACTACGCGAT
TCTCTACCGCGGCAACTACCAGGCGCGCATCTTCGAGCAGGCGCTGCGCAATCAGCGCATCCCCTACCAGATGGCCGGCG
GCCAGAGCTTCTTCGACAAGCCTGAGATCAAGGATCTGCTGGCTTACATGCGGCTGATCGCCAACCCCGACGACGATCCC
GCCTTCATCCGCGCGCTGACCACGCCCAAGCGCGGCGTCGGCGCCGGCACGCTGGAAAAACTGGGCGCCTGGGCCGGCCA
GTATGGCAAAAGCCTGTTCGCCGCCGCCCACGACGCCGGGCTGCGCGTCCAGGTGCAGCAGGCCCAGCTTGCGCCGCTGG
AGCAGTTCTGCGACTTCATCAGCCAGCTCCAATACCGAGCCACCCGCGAGGAAGCCGGCAAGCTTTCGCTGGAGCTGCTG
CAGGCCATCGGTTACGAAGCCTGGCTGTACGACAGCGAAGACAGTCCCAAGATCGCCGAAACCAAATGGAAGAACCTGCT
GGAAATGGTGGCCTGGCTGGCGAAGAAGGGCGAGGCCGACGGCAAGAACCTGATCGAGCTGACGCAGACCATCGCGCTGA
TCACCATGCTGGAAGGCCGCGACGAAGGCGAGGTGGACGCGGTGAGGATGTCCACGCTGCACGCGTCCAAGGGCCTGGAA
TACCCGCACGTGTTCCTGGTCGGCTGCGAGGAAGGCATCTTGCCGCACAGCGAGTCGGTGGAAAACGGCATGGTGGAAGA
GGAGCGCCGATTGATGTACGTCGGCATCACCCGCGCCCAGCGCAGCCTGACGCTGAGCTATTGCGTCAAGCGCCGCCGCG
CCGGCGAATGGCAGTTCATCGAACCGTCGCGCTTCATCAGCGAAATCGACGGCGAAGACCTGCGCCATTTCGGCAAACCC
GGCGCCGAACCGCTGGTCAGCAAAAGCGAAGGCAAATCCCGGCTGGCCAATCTGACGGCCATGCTGGCCGGCAAGGACAA
ATCTGGCGAGATGCCTGACTGA

Upstream 100 bases:

>100_bases
GCGGATCGAGCGCGCCTCATCCGCGATAAGCCATGATACCCCATGCTACAATGCATGGCCTTGATTACCGATTTGCTGCG
CCCGTCCGGGCCCGACCGCC

Downstream 100 bases:

>100_bases
ATCGGCAAGAAAAAATGGCGCCGCGAGGCGCCATAAAGAAGAATGAGGAACATCTCTGGGTGAAGCGAGGCTGGGGAACC
TCACCTGACTGAAGGGCTGG

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 673; Mature: 672

Protein sequence:

>673_residues
MSAPLLNPPQRAAIHYLDGPLLVLAGAGSGKTRVITYKIAHLVREGGIPARHIAAITFTNKAAREMLERVGKLMSSAEIR
GITVSTFHSLGMHILRQEAPHLGYKTQFSILDAYDAGKIIADILNTTSKDEIRKVQSQISLWKNDLKTPDDMLLAAQDQW
ESVCAKTYLAYQDTLTAYQAMDFDDLIRLPVQLFREHPEVLLKWQGKLRYLLIDEYQDTNTCQYQLVKLLTGVRGLFTAV
GDDDQSIYAWRGANMENLRLLQQDFPALKVIKLEQNYRSTARILRAANSVISNNPKLFEKQLWSELGLGEPIHVVQCKDE
EHEAETVVQRLLAHKFECRTEFKDYAILYRGNYQARIFEQALRNQRIPYQMAGGQSFFDKPEIKDLLAYMRLIANPDDDP
AFIRALTTPKRGVGAGTLEKLGAWAGQYGKSLFAAAHDAGLRVQVQQAQLAPLEQFCDFISQLQYRATREEAGKLSLELL
QAIGYEAWLYDSEDSPKIAETKWKNLLEMVAWLAKKGEADGKNLIELTQTIALITMLEGRDEGEVDAVRMSTLHASKGLE
YPHVFLVGCEEGILPHSESVENGMVEEERRLMYVGITRAQRSLTLSYCVKRRRAGEWQFIEPSRFISEIDGEDLRHFGKP
GAEPLVSKSEGKSRLANLTAMLAGKDKSGEMPD

Sequences:

>Translated_673_residues
MSAPLLNPPQRAAIHYLDGPLLVLAGAGSGKTRVITYKIAHLVREGGIPARHIAAITFTNKAAREMLERVGKLMSSAEIR
GITVSTFHSLGMHILRQEAPHLGYKTQFSILDAYDAGKIIADILNTTSKDEIRKVQSQISLWKNDLKTPDDMLLAAQDQW
ESVCAKTYLAYQDTLTAYQAMDFDDLIRLPVQLFREHPEVLLKWQGKLRYLLIDEYQDTNTCQYQLVKLLTGVRGLFTAV
GDDDQSIYAWRGANMENLRLLQQDFPALKVIKLEQNYRSTARILRAANSVISNNPKLFEKQLWSELGLGEPIHVVQCKDE
EHEAETVVQRLLAHKFECRTEFKDYAILYRGNYQARIFEQALRNQRIPYQMAGGQSFFDKPEIKDLLAYMRLIANPDDDP
AFIRALTTPKRGVGAGTLEKLGAWAGQYGKSLFAAAHDAGLRVQVQQAQLAPLEQFCDFISQLQYRATREEAGKLSLELL
QAIGYEAWLYDSEDSPKIAETKWKNLLEMVAWLAKKGEADGKNLIELTQTIALITMLEGRDEGEVDAVRMSTLHASKGLE
YPHVFLVGCEEGILPHSESVENGMVEEERRLMYVGITRAQRSLTLSYCVKRRRAGEWQFIEPSRFISEIDGEDLRHFGKP
GAEPLVSKSEGKSRLANLTAMLAGKDKSGEMPD
>Mature_672_residues
SAPLLNPPQRAAIHYLDGPLLVLAGAGSGKTRVITYKIAHLVREGGIPARHIAAITFTNKAAREMLERVGKLMSSAEIRG
ITVSTFHSLGMHILRQEAPHLGYKTQFSILDAYDAGKIIADILNTTSKDEIRKVQSQISLWKNDLKTPDDMLLAAQDQWE
SVCAKTYLAYQDTLTAYQAMDFDDLIRLPVQLFREHPEVLLKWQGKLRYLLIDEYQDTNTCQYQLVKLLTGVRGLFTAVG
DDDQSIYAWRGANMENLRLLQQDFPALKVIKLEQNYRSTARILRAANSVISNNPKLFEKQLWSELGLGEPIHVVQCKDEE
HEAETVVQRLLAHKFECRTEFKDYAILYRGNYQARIFEQALRNQRIPYQMAGGQSFFDKPEIKDLLAYMRLIANPDDDPA
FIRALTTPKRGVGAGTLEKLGAWAGQYGKSLFAAAHDAGLRVQVQQAQLAPLEQFCDFISQLQYRATREEAGKLSLELLQ
AIGYEAWLYDSEDSPKIAETKWKNLLEMVAWLAKKGEADGKNLIELTQTIALITMLEGRDEGEVDAVRMSTLHASKGLEY
PHVFLVGCEEGILPHSESVENGMVEEERRLMYVGITRAQRSLTLSYCVKRRRAGEWQFIEPSRFISEIDGEDLRHFGKPG
AEPLVSKSEGKSRLANLTAMLAGKDKSGEMPD

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=673, Percent_Identity=51.1144130757801, Blast_Score=655, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=636, Percent_Identity=37.2641509433962, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1787196, Length=343, Percent_Identity=26.8221574344023, Blast_Score=98, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322369, Length=456, Percent_Identity=31.359649122807, Blast_Score=181, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6324477, Length=674, Percent_Identity=22.8486646884273, Blast_Score=97, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 75869; Mature: 75738

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPLLNPPQRAAIHYLDGPLLVLAGAGSGKTRVITYKIAHLVREGGIPARHIAAITFTN
CCCCCCCCCHHHEEEEECCCEEEEEECCCCCEEEEHHHHHHHHHHCCCCHHHEEEEEECC
KAAREMLERVGKLMSSAEIRGITVSTFHSLGMHILRQEAPHLGYKTQFSILDAYDAGKII
HHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHH
ADILNTTSKDEIRKVQSQISLWKNDLKTPDDMLLAAQDQWESVCAKTYLAYQDTLTAYQA
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHEEECHHHHHHHHHHHHHHHHHHHHHHHH
MDFDDLIRLPVQLFREHPEVLLKWQGKLRYLLIDEYQDTNTCQYQLVKLLTGVRGLFTAV
CCHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHC
GDDDQSIYAWRGANMENLRLLQQDFPALKVIKLEQNYRSTARILRAANSVISNNPKLFEK
CCCCCCEEEECCCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHH
QLWSELGLGEPIHVVQCKDEEHEAETVVQRLLAHKFECRTEFKDYAILYRGNYQARIFEQ
HHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
ALRNQRIPYQMAGGQSFFDKPEIKDLLAYMRLIANPDDDPAFIRALTTPKRGVGAGTLEK
HHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHEEEECCCCCCCCCCHHHH
LGAWAGQYGKSLFAAAHDAGLRVQVQQAQLAPLEQFCDFISQLQYRATREEAGKLSLELL
HHHHHHHHHHHHHHHHCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAIGYEAWLYDSEDSPKIAETKWKNLLEMVAWLAKKGEADGKNLIELTQTIALITMLEGR
HHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
DEGEVDAVRMSTLHASKGLEYPHVFLVGCEEGILPHSESVENGMVEEERRLMYVGITRAQ
CCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHCCCHHHCCCEEEEEEEHHH
RSLTLSYCVKRRRAGEWQFIEPSRFISEIDGEDLRHFGKPGAEPLVSKSEGKSRLANLTA
HHHHHHHHHHHHCCCCCCEECHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
MLAGKDKSGEMPD
HHCCCCCCCCCCC
>Mature Secondary Structure 
SAPLLNPPQRAAIHYLDGPLLVLAGAGSGKTRVITYKIAHLVREGGIPARHIAAITFTN
CCCCCCCCHHHEEEEECCCEEEEEECCCCCEEEEHHHHHHHHHHCCCCHHHEEEEEECC
KAAREMLERVGKLMSSAEIRGITVSTFHSLGMHILRQEAPHLGYKTQFSILDAYDAGKII
HHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHH
ADILNTTSKDEIRKVQSQISLWKNDLKTPDDMLLAAQDQWESVCAKTYLAYQDTLTAYQA
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHEEECHHHHHHHHHHHHHHHHHHHHHHHH
MDFDDLIRLPVQLFREHPEVLLKWQGKLRYLLIDEYQDTNTCQYQLVKLLTGVRGLFTAV
CCHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHC
GDDDQSIYAWRGANMENLRLLQQDFPALKVIKLEQNYRSTARILRAANSVISNNPKLFEK
CCCCCCEEEECCCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHH
QLWSELGLGEPIHVVQCKDEEHEAETVVQRLLAHKFECRTEFKDYAILYRGNYQARIFEQ
HHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
ALRNQRIPYQMAGGQSFFDKPEIKDLLAYMRLIANPDDDPAFIRALTTPKRGVGAGTLEK
HHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHEEEECCCCCCCCCCHHHH
LGAWAGQYGKSLFAAAHDAGLRVQVQQAQLAPLEQFCDFISQLQYRATREEAGKLSLELL
HHHHHHHHHHHHHHHHCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAIGYEAWLYDSEDSPKIAETKWKNLLEMVAWLAKKGEADGKNLIELTQTIALITMLEGR
HHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
DEGEVDAVRMSTLHASKGLEYPHVFLVGCEEGILPHSESVENGMVEEERRLMYVGITRAQ
CCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHCCCHHHCCCEEEEEEEHHH
RSLTLSYCVKRRRAGEWQFIEPSRFISEIDGEDLRHFGKPGAEPLVSKSEGKSRLANLTA
HHHHHHHHHHHHCCCCCCEECHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
MLAGKDKSGEMPD
HHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]