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The map label for this gene is metB [H]

Identifier: 34499504

GI number: 34499504

Start: 4380032

End: 4381177

Strand: Reverse

Name: metB [H]

Synonym: CV_4049

Alternate gene names: 34499504

Gene position: 4381177-4380032 (Counterclockwise)

Preceding gene: 34499507

Following gene: 34499503

Centisome position: 92.21

GC content: 68.5

Gene sequence:

>1146_bases
GTGAAATTCGCTACCCGCGCCATCCATGTCGGCTACGACAATGCCGAACACAACCGCTCGGTGATGCCGCCGCTGTACCA
GACCTCGGCCTTCGCCTTCGACCATGTCGGCGAGGATTTGCGCTACGCCTACGCCCGCACCGGCAATCCGACCCGCAGCG
CGCTGCAGGACTGCCTGGCCAGCCTGGAAGAGGCCAGGCACGGCCTGGCCTTCTCCAGCGGCATGGCGGCGATAGACGCG
GTGCTGCGCGCCTGCCTGAAGCCGGGCGACGAGGTGATCGCGGTGGCGGATCTGTACGGCGGCGCTTACCGGCTGCTGAC
CCGGGTGATGCAGCCGGCCGGTATCAAGGTGAGCTTCGTGGACCTGTCCGACCCGGCGCGGCTGGAGGCGGCGATCACGC
CGGCCACCCGGCTGCTGTGGCTGGAGTCGCCGACCAATCCGCTGCTGGGCATGGTCGATATCGCCGCGCTGTCCGCGATC
GCCCATCGCCACAATGTAAAAGTGGCGGTGGACAATACCTTCGCCACGCCGTATCTGCAGCAGCCGCTGCTGCTCGGCGC
CGACATCGTCGTGCACTCGGCCACCAAGTACCTGGGCGGGCACTCCGACGTGCTGCTGGGCCTGGTGGCGGTCAGCGACG
ACAAGCTGCACCACGATATCCGCTTCCTGCAGAACGCCGCCGGCGCGGTGCCCGGGCCGCAGGATTGCTTCCTGACGCTG
CGCGGCATCAAGACGCTGGCGCTGCGGATGGAGCGCCATTGCGACAGCGCGGAGAAAGTGGCGGTCTGGCTGGCCGGACA
GCCGGCCGTGGCCAAGGTGTTCTACCCCGGACTCCCGGAGCATCCGGGGCATGAGCTGGCGAAGCGGCAGATGCGGCGCT
TCGGCGGCATCATCAGCATCCGGCTGCAGGACGACAGCCGCGAGGCGGCCAGCCGCGTCGCGCAGAGGCTGAAGCTGTTT
GCGCTGGCGGAGTCGCTGGGCGGGGTGGAGTCGCTGGTCAACCATAGCTACACGATGTCGCACGGCGGCATGCCGGCCGA
GCAGAAGGCGGCGCTGGGCATTGTCGAGGGCGGCCTGCGGCTGTCGATCGGCATCGAGGATCTGGACGACATCCTGGCGG
ACCTGGAGCAGGCGCTGCGCGATTGA

Upstream 100 bases:

>100_bases
GCATCGTGATGAAATGAGCATGGCAAATGCATGATTTGCAAGGAGTTAGGGTTTGATTGTCGGGCAATTTGGCCCAAGCT
CATGATTTAGAGGGATTTTC

Downstream 100 bases:

>100_bases
ACGGTCCGGGCGCGGGGCCCGGATGGCTGGCAAGATCCAATAACAAGGAGAATGCGGATGTCGGCGGCGAAATGGCAGCC
ATTGCCACTGAAACTTCCGG

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MKFATRAIHVGYDNAEHNRSVMPPLYQTSAFAFDHVGEDLRYAYARTGNPTRSALQDCLASLEEARHGLAFSSGMAAIDA
VLRACLKPGDEVIAVADLYGGAYRLLTRVMQPAGIKVSFVDLSDPARLEAAITPATRLLWLESPTNPLLGMVDIAALSAI
AHRHNVKVAVDNTFATPYLQQPLLLGADIVVHSATKYLGGHSDVLLGLVAVSDDKLHHDIRFLQNAAGAVPGPQDCFLTL
RGIKTLALRMERHCDSAEKVAVWLAGQPAVAKVFYPGLPEHPGHELAKRQMRRFGGIISIRLQDDSREAASRVAQRLKLF
ALAESLGGVESLVNHSYTMSHGGMPAEQKAALGIVEGGLRLSIGIEDLDDILADLEQALRD

Sequences:

>Translated_381_residues
MKFATRAIHVGYDNAEHNRSVMPPLYQTSAFAFDHVGEDLRYAYARTGNPTRSALQDCLASLEEARHGLAFSSGMAAIDA
VLRACLKPGDEVIAVADLYGGAYRLLTRVMQPAGIKVSFVDLSDPARLEAAITPATRLLWLESPTNPLLGMVDIAALSAI
AHRHNVKVAVDNTFATPYLQQPLLLGADIVVHSATKYLGGHSDVLLGLVAVSDDKLHHDIRFLQNAAGAVPGPQDCFLTL
RGIKTLALRMERHCDSAEKVAVWLAGQPAVAKVFYPGLPEHPGHELAKRQMRRFGGIISIRLQDDSREAASRVAQRLKLF
ALAESLGGVESLVNHSYTMSHGGMPAEQKAALGIVEGGLRLSIGIEDLDDILADLEQALRD
>Mature_381_residues
MKFATRAIHVGYDNAEHNRSVMPPLYQTSAFAFDHVGEDLRYAYARTGNPTRSALQDCLASLEEARHGLAFSSGMAAIDA
VLRACLKPGDEVIAVADLYGGAYRLLTRVMQPAGIKVSFVDLSDPARLEAAITPATRLLWLESPTNPLLGMVDIAALSAI
AHRHNVKVAVDNTFATPYLQQPLLLGADIVVHSATKYLGGHSDVLLGLVAVSDDKLHHDIRFLQNAAGAVPGPQDCFLTL
RGIKTLALRMERHCDSAEKVAVWLAGQPAVAKVFYPGLPEHPGHELAKRQMRRFGGIISIRLQDDSREAASRVAQRLKLF
ALAESLGGVESLVNHSYTMSHGGMPAEQKAALGIVEGGLRLSIGIEDLDDILADLEQALRD

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=380, Percent_Identity=46.8421052631579, Blast_Score=333, Evalue=1e-91,
Organism=Homo sapiens, GI299473759, Length=380, Percent_Identity=42.8947368421053, Blast_Score=288, Evalue=4e-78,
Organism=Homo sapiens, GI25453487, Length=379, Percent_Identity=41.688654353562, Blast_Score=265, Evalue=4e-71,
Organism=Escherichia coli, GI1790375, Length=379, Percent_Identity=43.7994722955145, Blast_Score=304, Evalue=6e-84,
Organism=Escherichia coli, GI1789383, Length=389, Percent_Identity=29.305912596401, Blast_Score=158, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI17538055, Length=382, Percent_Identity=46.8586387434555, Blast_Score=328, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI17560056, Length=382, Percent_Identity=45.5497382198953, Blast_Score=320, Evalue=7e-88,
Organism=Caenorhabditis elegans, GI71980708, Length=370, Percent_Identity=36.4864864864865, Blast_Score=249, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6319307, Length=389, Percent_Identity=43.4447300771208, Blast_Score=313, Evalue=4e-86,
Organism=Saccharomyces cerevisiae, GI6323333, Length=433, Percent_Identity=31.6397228637413, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6321254, Length=405, Percent_Identity=33.3333333333333, Blast_Score=180, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6321170, Length=324, Percent_Identity=31.7901234567901, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6321904, Length=400, Percent_Identity=27.5, Blast_Score=123, Evalue=4e-29,
Organism=Drosophila melanogaster, GI19922554, Length=381, Percent_Identity=44.8818897637795, Blast_Score=347, Evalue=7e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 41147; Mature: 41147

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFATRAIHVGYDNAEHNRSVMPPLYQTSAFAFDHVGEDLRYAYARTGNPTRSALQDCLA
CCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHH
SLEEARHGLAFSSGMAAIDAVLRACLKPGDEVIAVADLYGGAYRLLTRVMQPAGIKVSFV
HHHHHHCCCCHHCCHHHHHHHHHHHCCCCCCEEEEEHHCCCHHHHHHHHHCCCCCEEEEE
DLSDPARLEAAITPATRLLWLESPTNPLLGMVDIAALSAIAHRHNVKVAVDNTFATPYLQ
ECCCCCHHEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHH
QPLLLGADIVVHSATKYLGGHSDVLLGLVAVSDDKLHHDIRFLQNAAGAVPGPQDCFLTL
CCHHHCCHHHHHHHHHHHCCCCHHHEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHH
RGIKTLALRMERHCDSAEKVAVWLAGQPAVAKVFYPGLPEHPGHELAKRQMRRFGGIISI
HHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCEEEE
RLQDDSREAASRVAQRLKLFALAESLGGVESLVNHSYTMSHGGMPAEQKAALGIVEGGLR
EECCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCEEECCCCCCCHHHHHHEEEECCEE
LSIGIEDLDDILADLEQALRD
EEECHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKFATRAIHVGYDNAEHNRSVMPPLYQTSAFAFDHVGEDLRYAYARTGNPTRSALQDCLA
CCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHH
SLEEARHGLAFSSGMAAIDAVLRACLKPGDEVIAVADLYGGAYRLLTRVMQPAGIKVSFV
HHHHHHCCCCHHCCHHHHHHHHHHHCCCCCCEEEEEHHCCCHHHHHHHHHCCCCCEEEEE
DLSDPARLEAAITPATRLLWLESPTNPLLGMVDIAALSAIAHRHNVKVAVDNTFATPYLQ
ECCCCCHHEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHH
QPLLLGADIVVHSATKYLGGHSDVLLGLVAVSDDKLHHDIRFLQNAAGAVPGPQDCFLTL
CCHHHCCHHHHHHHHHHHCCCCHHHEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHH
RGIKTLALRMERHCDSAEKVAVWLAGQPAVAKVFYPGLPEHPGHELAKRQMRRFGGIISI
HHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCEEEE
RLQDDSREAASRVAQRLKLFALAESLGGVESLVNHSYTMSHGGMPAEQKAALGIVEGGLR
EECCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCEEECCCCCCCHHHHHHEEEECCEE
LSIGIEDLDDILADLEQALRD
EEECHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]