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The map label for this gene is pleD [H]

Identifier: 34499496

GI number: 34499496

Start: 4373868

End: 4374845

Strand: Reverse

Name: pleD [H]

Synonym: CV_4041

Alternate gene names: 34499496

Gene position: 4374845-4373868 (Counterclockwise)

Preceding gene: 34499497

Following gene: 34499493

Centisome position: 92.08

GC content: 68.2

Gene sequence:

>978_bases
ATGGGCGGCGGCGGGCCGATGGAGATCGGCCCGGATGCCGCCGGATGCCAGTGTATGAAGCCTGCCGATGAAGACGAAGA
CATCGCCTCCCGCCTGATGTCGCTGCAACGGGATTGCCCGTTGCTGATCTCCCTGTTCGACGAGCGCGACCGCCTGCGCT
ACGCCAATCCGGCGTTCTGCGCGGCGCTGGGCGTGGCGGCGGACGAGCGCGTCAGCTGGGAGCAACTGATGCGGCGCAAT
TATCAAAACCGCCAGGGCACGCAGATCAGCACCCGGGACTTCGAGGCTTGGCTGGCGTCGGCGAAGTCGCGCCGGGCCAA
GCGGCCCTTCCGCGCTTTCGAGACCGACCTGGCCGACGGCCGCTGGATCTGGATGACCGAGACCACGCAGACCGGCGGCT
GGATGCTGTGCGTCGGCAGCGAGATCACCGAGCTGCGCGTCGGCGAGCGCGAGCTCAGGCAGGCGCGCGACATGGCGCAG
CGCGCGGCGCTGACCGACCCCCTGACCGGCATCGGCAACCGCGGCCACATCCTGCAGCAGATGGACGGCCTGGTGGAGCA
GTTTCATGGCGGAGCGTGCCAGGCGGCCGCGGTGGCCATCCTGGATCTGGACAACTTCAAGCTGATCAACGACCTGAAGG
GCCATCTGGCCGGCGACGCGGTGCTGCGCGATTTCGCGCATAGGCTGCAGCCCTTGCTGAAACCGCATGACAGCCTGGGC
CGGCTGGGCGGGGAGGAGTTTCTGCTGCTGTTGCCCGGCAGCGGCCTGGAGCAGGCGGAGGCCTTGATCCGGACCATACT
TTCCGATGTGCAGTCGTCGCGGCCGCTGGCGGATGACCCGGGGTTTTCCTACACCTGTTCGATCGGGATGTCCTTGCTGC
GCGCCGGCGACGATCTGTCGGCGCCGCTGGGCCGGGCCGACGAAGCCTTGTATCAAGCCAAGGCCGGCGGGCGCAATACC
TGGCGCGTGCGGCTGTAA

Upstream 100 bases:

>100_bases
GCCTGCTGGCGGCGGGGCAGAAATAAGCTGTTCTGACCCGGAGGACAGCGCTTAATCCCGCCCGGTTTTGCTCTACCATG
GCTTATGACCGCCGTCCGCA

Downstream 100 bases:

>100_bases
GGCGTTTGGGCATGAAAAAAGGCGGGATCGCTCCCGCCTTTGTCGTTTCCGGCTGCCCGTCTTATTGCTTGACGCAGTCC
ACGCAGTACACCTTGCGGTC

Product: hypothetical protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MGGGGPMEIGPDAAGCQCMKPADEDEDIASRLMSLQRDCPLLISLFDERDRLRYANPAFCAALGVAADERVSWEQLMRRN
YQNRQGTQISTRDFEAWLASAKSRRAKRPFRAFETDLADGRWIWMTETTQTGGWMLCVGSEITELRVGERELRQARDMAQ
RAALTDPLTGIGNRGHILQQMDGLVEQFHGGACQAAAVAILDLDNFKLINDLKGHLAGDAVLRDFAHRLQPLLKPHDSLG
RLGGEEFLLLLPGSGLEQAEALIRTILSDVQSSRPLADDPGFSYTCSIGMSLLRAGDDLSAPLGRADEALYQAKAGGRNT
WRVRL

Sequences:

>Translated_325_residues
MGGGGPMEIGPDAAGCQCMKPADEDEDIASRLMSLQRDCPLLISLFDERDRLRYANPAFCAALGVAADERVSWEQLMRRN
YQNRQGTQISTRDFEAWLASAKSRRAKRPFRAFETDLADGRWIWMTETTQTGGWMLCVGSEITELRVGERELRQARDMAQ
RAALTDPLTGIGNRGHILQQMDGLVEQFHGGACQAAAVAILDLDNFKLINDLKGHLAGDAVLRDFAHRLQPLLKPHDSLG
RLGGEEFLLLLPGSGLEQAEALIRTILSDVQSSRPLADDPGFSYTCSIGMSLLRAGDDLSAPLGRADEALYQAKAGGRNT
WRVRL
>Mature_324_residues
GGGGPMEIGPDAAGCQCMKPADEDEDIASRLMSLQRDCPLLISLFDERDRLRYANPAFCAALGVAADERVSWEQLMRRNY
QNRQGTQISTRDFEAWLASAKSRRAKRPFRAFETDLADGRWIWMTETTQTGGWMLCVGSEITELRVGERELRQARDMAQR
AALTDPLTGIGNRGHILQQMDGLVEQFHGGACQAAAVAILDLDNFKLINDLKGHLAGDAVLRDFAHRLQPLLKPHDSLGR
LGGEEFLLLLPGSGLEQAEALIRTILSDVQSSRPLADDPGFSYTCSIGMSLLRAGDDLSAPLGRADEALYQAKAGGRNTW
RVRL

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787541, Length=235, Percent_Identity=31.4893617021277, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87081881, Length=190, Percent_Identity=36.8421052631579, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787262, Length=178, Percent_Identity=35.3932584269663, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788381, Length=184, Percent_Identity=33.1521739130435, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787816, Length=154, Percent_Identity=31.8181818181818, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87082007, Length=167, Percent_Identity=37.125748502994, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87081977, Length=171, Percent_Identity=31.5789473684211, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786584, Length=192, Percent_Identity=30.2083333333333, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI145693134, Length=130, Percent_Identity=33.8461538461538, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1788956, Length=172, Percent_Identity=34.3023255813954, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788085, Length=189, Percent_Identity=29.6296296296296, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787802, Length=136, Percent_Identity=34.5588235294118, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 35770; Mature: 35639

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGGGPMEIGPDAAGCQCMKPADEDEDIASRLMSLQRDCPLLISLFDERDRLRYANPAFC
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCHHHHCCCCHHHH
AALGVAADERVSWEQLMRRNYQNRQGTQISTRDFEAWLASAKSRRAKRPFRAFETDLADG
HHHHCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCC
RWIWMTETTQTGGWMLCVGSEITELRVGERELRQARDMAQRAALTDPLTGIGNRGHILQQ
EEEEEEECCCCCCEEEEECCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHCCCCCCHHHHH
MDGLVEQFHGGACQAAAVAILDLDNFKLINDLKGHLAGDAVLRDFAHRLQPLLKPHDSLG
HHHHHHHHCCCCHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
RLGGEEFLLLLPGSGLEQAEALIRTILSDVQSSRPLADDPGFSYTCSIGMSLLRAGDDLS
HCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHCCCCCC
APLGRADEALYQAKAGGRNTWRVRL
CCCCHHHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure 
GGGGPMEIGPDAAGCQCMKPADEDEDIASRLMSLQRDCPLLISLFDERDRLRYANPAFC
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCHHHHCCCCHHHH
AALGVAADERVSWEQLMRRNYQNRQGTQISTRDFEAWLASAKSRRAKRPFRAFETDLADG
HHHHCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCC
RWIWMTETTQTGGWMLCVGSEITELRVGERELRQARDMAQRAALTDPLTGIGNRGHILQQ
EEEEEEECCCCCCEEEEECCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHCCCCCCHHHHH
MDGLVEQFHGGACQAAAVAILDLDNFKLINDLKGHLAGDAVLRDFAHRLQPLLKPHDSLG
HHHHHHHHCCCCHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
RLGGEEFLLLLPGSGLEQAEALIRTILSDVQSSRPLADDPGFSYTCSIGMSLLRAGDDLS
HCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHCCCCCC
APLGRADEALYQAKAGGRNTWRVRL
CCCCHHHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA