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The map label for this gene is leuE [H]

Identifier: 34499497

GI number: 34499497

Start: 4374920

End: 4375549

Strand: Reverse

Name: leuE [H]

Synonym: CV_4042

Alternate gene names: 34499497

Gene position: 4375549-4374920 (Counterclockwise)

Preceding gene: 34499500

Following gene: 34499496

Centisome position: 92.1

GC content: 70.0

Gene sequence:

>630_bases
ATGCCCACCTTGTCCACTCTGCTGCTGTTCTCCCTCGCCTGTCTCGCGCTGGCCGCCACGCCCGGCCCCGACATGCTGCT
GATCGCCAGCCGCAGCGCAGGGCAGGGCCTGCGCGCCGGCTTCGCCACCTTGTTCGGCATCCAGCTGGGCACCTATTGCC
ACGCGCTGCTGGCGGCGCTGGGTCTGGCCCAGCTGTTCGTGCTGGTGCCGGTGGCCTACGACGCGGTGCGCGTCGCCGGC
GCCGCCTACCTGCTTTACCTGGCCTGGACCACCTTGCGCAGCAACAGCGTGCTGCGCGCGGCCGATCCCGCCGCCGCCAA
CCGGCCGCTGGCGCGCATCGCGCTGCAGGGGCTGACCACCAATCTGCTCAATCCCAAGATGGCGCTGTTCGTGCTGGCGC
TGTTTCCGCAGTTCGTCGAGCCGCAGGCCGGTCCGGTGGCGCTGCAGATTATGTTGCTGGCTACGGTACTGAACGCGGTC
GGCTTTGTCGTCAACGCGCTGGTGATCGTCGCCAGCAGCCGCATGGGCCGGATGCTGGCCGGCGGCGCGAGCGGGCGCTG
GCCGCAATACCTGCTGGCGACGGTGTTTGGCGGGCTGGCTGTGCGCCTGCTGGCGGCGGGGCAGAAATAA

Upstream 100 bases:

>100_bases
CTAAACGCCGCCGCCTATCAAACCATGGCGGCATGCTTGTTCGCGCCGGAATGATATCGGTATGATGAATCCCTTCCTCA
CCGTTTGCGGAGCGCCGCGC

Downstream 100 bases:

>100_bases
GCTGTTCTGACCCGGAGGACAGCGCTTAATCCCGCCCGGTTTTGCTCTACCATGGCTTATGACCGCCGTCCGCAATGGGC
GGCGGCGGGCCGATGGAGAT

Product: amino acid efflux protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MPTLSTLLLFSLACLALAATPGPDMLLIASRSAGQGLRAGFATLFGIQLGTYCHALLAALGLAQLFVLVPVAYDAVRVAG
AAYLLYLAWTTLRSNSVLRAADPAAANRPLARIALQGLTTNLLNPKMALFVLALFPQFVEPQAGPVALQIMLLATVLNAV
GFVVNALVIVASSRMGRMLAGGASGRWPQYLLATVFGGLAVRLLAAGQK

Sequences:

>Translated_209_residues
MPTLSTLLLFSLACLALAATPGPDMLLIASRSAGQGLRAGFATLFGIQLGTYCHALLAALGLAQLFVLVPVAYDAVRVAG
AAYLLYLAWTTLRSNSVLRAADPAAANRPLARIALQGLTTNLLNPKMALFVLALFPQFVEPQAGPVALQIMLLATVLNAV
GFVVNALVIVASSRMGRMLAGGASGRWPQYLLATVFGGLAVRLLAAGQK
>Mature_208_residues
PTLSTLLLFSLACLALAATPGPDMLLIASRSAGQGLRAGFATLFGIQLGTYCHALLAALGLAQLFVLVPVAYDAVRVAGA
AYLLYLAWTTLRSNSVLRAADPAAANRPLARIALQGLTTNLLNPKMALFVLALFPQFVEPQAGPVALQIMLLATVLNAVG
FVVNALVIVASSRMGRMLAGGASGRWPQYLLATVFGGLAVRLLAAGQK

Specific function: Exporter of leucine [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=199, Percent_Identity=29.1457286432161, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788099, Length=189, Percent_Identity=28.042328042328, Blast_Score=71, Evalue=4e-14,
Organism=Escherichia coli, GI1786522, Length=119, Percent_Identity=31.9327731092437, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21715; Mature: 21584

Theoretical pI: Translated: 11.00; Mature: 11.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTLSTLLLFSLACLALAATPGPDMLLIASRSAGQGLRAGFATLFGIQLGTYCHALLAAL
CCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GLAQLFVLVPVAYDAVRVAGAAYLLYLAWTTLRSNSVLRAADPAAANRPLARIALQGLTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHH
NLLNPKMALFVLALFPQFVEPQAGPVALQIMLLATVLNAVGFVVNALVIVASSRMGRMLA
HHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGASGRWPQYLLATVFGGLAVRLLAAGQK
CCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PTLSTLLLFSLACLALAATPGPDMLLIASRSAGQGLRAGFATLFGIQLGTYCHALLAAL
CHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GLAQLFVLVPVAYDAVRVAGAAYLLYLAWTTLRSNSVLRAADPAAANRPLARIALQGLTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHH
NLLNPKMALFVLALFPQFVEPQAGPVALQIMLLATVLNAVGFVVNALVIVASSRMGRMLA
HHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGASGRWPQYLLATVFGGLAVRLLAAGQK
CCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA