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The map label for this gene is rfbA [H]

Identifier: 34499467

GI number: 34499467

Start: 4338401

End: 4339294

Strand: Direct

Name: rfbA [H]

Synonym: CV_4012

Alternate gene names: 34499467

Gene position: 4338401-4339294 (Clockwise)

Preceding gene: 34499466

Following gene: 34499468

Centisome position: 91.31

GC content: 61.3

Gene sequence:

>894_bases
ATGACCACAGGAAATCTGGCAATGAAACGAAAAGGCATCATTCTGGCCGGAGGCTCCGGCACCCGGCTCTACCCGGCCAC
CATCGCCGTCAGCAAGCAATTGCTGCCGATCTACGACAAGCCGATGATCTATTACCCGCTGACGACGCTGATGCTGGCCG
GCATTCAGGACATCCTGATCATCTCCACGCCGCAGGACACGCCCCGCTTCCAGCAACTGCTGGGAGACGGCAGTCAATGG
GGGATCAATCTGCAATACGCGGTCCAACCCAGTCCGGACGGCCTGGCCCAGGCCTTCCTGATCGGCGAGCGCTTCATCGA
CGGGTCACCGTCTGCGCTGGTGCTCGGCGACAACATCTTCTACGGCCATGAATTTTCCGGTCTGCTGCAGGCGGCCAATA
TCCAGACCTCCGGCGCGACCGTCTTCGCCTACCGGGTCAGCGACCCCGAACGCTACGGCGTGGTGGAGTTCGACCAGCAA
GGCTGCGCCCTGAGCATCGAAGAGAAACCCGCCCAGCCCAAATCGCACTACGCGGTGACCGGCCTGTACTTCTACGACTC
GCAGGTGGTAGACATCGCGAAGCGCATCAAGCCCTCGCCCCGAGGCGAACTGGAAATCACCGACGTCAACAATATTTACC
TGCAACAGCAGCAGTTGAACGTGCAGACCATGGGGCGCGGCTATGCCTGGCTGGATACCGGCACCCATGAATCGTTGCTG
GAGGCCGGCCAGTTCATCGCCACCATAGAGAACCGCCAGGGACAAAAGGTGGCCTGCCCCGAGGAGATCGCCTTCCGCAA
CGGCTGGATAGACGAAGCCCAGGTACGCCAGCTGGCCCAGCCCTTGCAGAAAACCGGCTACGGCCGCTATCTGCTGGCGA
TGCTCAGCAACTGA

Upstream 100 bases:

>100_bases
AAAGCGCTTTCGGCATCGAGCTGCCGGAATGGAGAAGCGGCGTGGACCAGGTGCTGGCTCAATTGCTCCCCACCTACGAC
AGCGGTGCCCCAGCCCGGGA

Downstream 100 bases:

>100_bases
CACCCTACACGAACAGAACAAAGACAAGCGATGAAAGTCATTGATACCGAGATCTCCGCGGTCAAGATCATCGAGCCCCA
GATATTTGGCGATGAGCGCG

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: G1P-TT 1; dTDP-glucose pyrophosphorylase 1; dTDP-glucose synthase 1 [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MTTGNLAMKRKGIILAGGSGTRLYPATIAVSKQLLPIYDKPMIYYPLTTLMLAGIQDILIISTPQDTPRFQQLLGDGSQW
GINLQYAVQPSPDGLAQAFLIGERFIDGSPSALVLGDNIFYGHEFSGLLQAANIQTSGATVFAYRVSDPERYGVVEFDQQ
GCALSIEEKPAQPKSHYAVTGLYFYDSQVVDIAKRIKPSPRGELEITDVNNIYLQQQQLNVQTMGRGYAWLDTGTHESLL
EAGQFIATIENRQGQKVACPEEIAFRNGWIDEAQVRQLAQPLQKTGYGRYLLAMLSN

Sequences:

>Translated_297_residues
MTTGNLAMKRKGIILAGGSGTRLYPATIAVSKQLLPIYDKPMIYYPLTTLMLAGIQDILIISTPQDTPRFQQLLGDGSQW
GINLQYAVQPSPDGLAQAFLIGERFIDGSPSALVLGDNIFYGHEFSGLLQAANIQTSGATVFAYRVSDPERYGVVEFDQQ
GCALSIEEKPAQPKSHYAVTGLYFYDSQVVDIAKRIKPSPRGELEITDVNNIYLQQQQLNVQTMGRGYAWLDTGTHESLL
EAGQFIATIENRQGQKVACPEEIAFRNGWIDEAQVRQLAQPLQKTGYGRYLLAMLSN
>Mature_296_residues
TTGNLAMKRKGIILAGGSGTRLYPATIAVSKQLLPIYDKPMIYYPLTTLMLAGIQDILIISTPQDTPRFQQLLGDGSQWG
INLQYAVQPSPDGLAQAFLIGERFIDGSPSALVLGDNIFYGHEFSGLLQAANIQTSGATVFAYRVSDPERYGVVEFDQQG
CALSIEEKPAQPKSHYAVTGLYFYDSQVVDIAKRIKPSPRGELEITDVNNIYLQQQQLNVQTMGRGYAWLDTGTHESLLE
AGQFIATIENRQGQKVACPEEIAFRNGWIDEAQVRQLAQPLQKTGYGRYLLAMLSN

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788351, Length=288, Percent_Identity=76.0416666666667, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI1790224, Length=285, Percent_Identity=67.0175438596491, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1787488, Length=298, Percent_Identity=24.1610738255034, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI133931050, Length=182, Percent_Identity=25.8241758241758, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320148, Length=276, Percent_Identity=27.536231884058, Blast_Score=89, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21355443, Length=249, Percent_Identity=25.7028112449799, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24644084, Length=249, Percent_Identity=25.7028112449799, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 32772; Mature: 32641

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTGNLAMKRKGIILAGGSGTRLYPATIAVSKQLLPIYDKPMIYYPLTTLMLAGIQDILI
CCCCCEEEEECCEEEECCCCCEEEEEEEEECCCCCCCCCCCEEEECHHHHHHHCCCEEEE
ISTPQDTPRFQQLLGDGSQWGINLQYAVQPSPDGLAQAFLIGERFIDGSPSALVLGDNIF
EECCCCCHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEECCEE
YGHEFSGLLQAANIQTSGATVFAYRVSDPERYGVVEFDQQGCALSIEEKPAQPKSHYAVT
ECCCHHHHHEECCEECCCCEEEEEECCCCCCCCEEEECCCCCEEEECCCCCCCCCCEEEE
GLYFYDSQVVDIAKRIKPSPRGELEITDVNNIYLQQQQLNVQTMGRGYAWLDTGTHESLL
EEEEECCHHHHHHHHCCCCCCCCEEEEECCCEEEEHHHCCEEECCCCEEEEECCCHHHHH
EAGQFIATIENRQGQKVACPEEIAFRNGWIDEAQVRQLAQPLQKTGYGRYLLAMLSN
HHCCEEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECC
>Mature Secondary Structure 
TTGNLAMKRKGIILAGGSGTRLYPATIAVSKQLLPIYDKPMIYYPLTTLMLAGIQDILI
CCCCEEEEECCEEEECCCCCEEEEEEEEECCCCCCCCCCCEEEECHHHHHHHCCCEEEE
ISTPQDTPRFQQLLGDGSQWGINLQYAVQPSPDGLAQAFLIGERFIDGSPSALVLGDNIF
EECCCCCHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEECCEE
YGHEFSGLLQAANIQTSGATVFAYRVSDPERYGVVEFDQQGCALSIEEKPAQPKSHYAVT
ECCCHHHHHEECCEECCCCEEEEEECCCCCCCCEEEECCCCCEEEECCCCCCCCCCEEEE
GLYFYDSQVVDIAKRIKPSPRGELEITDVNNIYLQQQQLNVQTMGRGYAWLDTGTHESLL
EEEEECCHHHHHHHHCCCCCCCCEEEEECCCEEEEHHHCCEEECCCCEEEEECCCHHHHH
EAGQFIATIENRQGQKVACPEEIAFRNGWIDEAQVRQLAQPLQKTGYGRYLLAMLSN
HHCCEEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7517391; 9097040; 9278503; 7517390 [H]