Click here to switch to the map view.

The map label for this gene is rmlB [H]

Identifier: 34499465

GI number: 34499465

Start: 4336417

End: 4337475

Strand: Direct

Name: rmlB [H]

Synonym: CV_4010

Alternate gene names: 34499465

Gene position: 4336417-4337475 (Clockwise)

Preceding gene: 34499458

Following gene: 34499466

Centisome position: 91.27

GC content: 61.66

Gene sequence:

>1059_bases
ATGACCATTCTCGTTACCGGCGGCGCCGGCTTCATCGGCGGCAACTTCGTTCTCGACTGGCTAGCAGAACGGGAAGAGAC
TGTCATCAATCTGGATGCGCTGACTTACGCCGGCAATCTGGAAACCCTGCACACGGTCAGGACCAAGCCCAACCATGTTT
TCACGCATGGCGATATCGGCGACCGGAAACTGGTCGCCGAGTTGCTGCGCACCCATCGCCCTCGCGCCATTGTCAACTTC
GCCGCGGAAAGCCACGTCGACCGCTCGATACACGGACCCGGCGACTTCATCCAGACCAATATCGTCGGCACCTTCAATCT
GCTGGAGTGCGCGCGCGAATACTGGAGCGGACTGGAAGGCGGCGATCGCGAAGCCTTCCGCTTCCTGCACGTTTCCACCG
ACGAGGTATATGGCACCCTGCGCGCGGAGGATGCTCCGTTCTCGGAAACCAACCGCTACGAGCCGAATAGCCCCTATTCC
GCCTCCAAGGCCGCCTCGGACCACCTGGTGCGCGCCTGGCACCACACCTACGGCCTGCCAGTGCTGACCACCAACTGCAG
CAATAATTACGGCCCCTATCACTTCCCGGAAAAGCTGATTCCGCTGGTCATCCTGAACGCCCTCGCCGGCAAGCCGCTGC
CGATCTACGGCGACGGCCAGCAGGTGCGCGACTGGCTCTACGTCAAGGACCACTGCAGCGCGATCCGCCGCGTGCTGGAA
GCCGGCAAGCTCGGCGAAACCTATAATGTCGGCGGCTGGAACGAAAAAACCAATCTCGAGGTAGTCAACACCATCTGCGG
CATTCTGGACCAGTTGAGGCCCCGCGCGGATGGCAGGCCCTACCGCGAGCAGATCCACTTCGTCTCCGACCGGCCCGGCC
ACGACCGCCGCTATGCGATCGACGCCCGCAAGCTGGAGCGGGAACTGGGATGGAAGCCCGCCGAAACTTTCGACAGCGGC
ATCCGCAAGACCATTGAATGGTATCTGGGCAATCCAAACTGGATCGAACACGTCAGCAACGGCAGCTACCGCGACTGGCT
GGACAAGCACTACGGATGA

Upstream 100 bases:

>100_bases
GCTATCGCTTTTGCCGGCATCCCCGCAGTGCCCGGCGGTGCCTAATACCGGGTTTCACGGCCGCAAGACAGCGGCGATCC
CGATCATACAGGAAGCTTCC

Downstream 100 bases:

>100_bases
TGACCATGCCCCGCATACTGATAACCGGCAAAAACGGCCAGGTCGGCCACGAATTGCTGCGCGCGCTGGCGCCGCTGGGC
GAGCTGATCGCCACCGACAG

Product: dtdpglucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MTILVTGGAGFIGGNFVLDWLAEREETVINLDALTYAGNLETLHTVRTKPNHVFTHGDIGDRKLVAELLRTHRPRAIVNF
AAESHVDRSIHGPGDFIQTNIVGTFNLLECAREYWSGLEGGDREAFRFLHVSTDEVYGTLRAEDAPFSETNRYEPNSPYS
ASKAASDHLVRAWHHTYGLPVLTTNCSNNYGPYHFPEKLIPLVILNALAGKPLPIYGDGQQVRDWLYVKDHCSAIRRVLE
AGKLGETYNVGGWNEKTNLEVVNTICGILDQLRPRADGRPYREQIHFVSDRPGHDRRYAIDARKLERELGWKPAETFDSG
IRKTIEWYLGNPNWIEHVSNGSYRDWLDKHYG

Sequences:

>Translated_352_residues
MTILVTGGAGFIGGNFVLDWLAEREETVINLDALTYAGNLETLHTVRTKPNHVFTHGDIGDRKLVAELLRTHRPRAIVNF
AAESHVDRSIHGPGDFIQTNIVGTFNLLECAREYWSGLEGGDREAFRFLHVSTDEVYGTLRAEDAPFSETNRYEPNSPYS
ASKAASDHLVRAWHHTYGLPVLTTNCSNNYGPYHFPEKLIPLVILNALAGKPLPIYGDGQQVRDWLYVKDHCSAIRRVLE
AGKLGETYNVGGWNEKTNLEVVNTICGILDQLRPRADGRPYREQIHFVSDRPGHDRRYAIDARKLERELGWKPAETFDSG
IRKTIEWYLGNPNWIEHVSNGSYRDWLDKHYG
>Mature_351_residues
TILVTGGAGFIGGNFVLDWLAEREETVINLDALTYAGNLETLHTVRTKPNHVFTHGDIGDRKLVAELLRTHRPRAIVNFA
AESHVDRSIHGPGDFIQTNIVGTFNLLECAREYWSGLEGGDREAFRFLHVSTDEVYGTLRAEDAPFSETNRYEPNSPYSA
SKAASDHLVRAWHHTYGLPVLTTNCSNNYGPYHFPEKLIPLVILNALAGKPLPIYGDGQQVRDWLYVKDHCSAIRRVLEA
GKLGETYNVGGWNEKTNLEVVNTICGILDQLRPRADGRPYREQIHFVSDRPGHDRRYAIDARKLERELGWKPAETFDSGI
RKTIEWYLGNPNWIEHVSNGSYRDWLDKHYG

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=334, Percent_Identity=35.6287425149701, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI42516563, Length=348, Percent_Identity=25, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI4504031, Length=357, Percent_Identity=24.6498599439776, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI1788353, Length=359, Percent_Identity=59.8885793871866, Blast_Score=450, Evalue=1e-128,
Organism=Escherichia coli, GI48994969, Length=347, Percent_Identity=59.6541786743516, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI1786974, Length=359, Percent_Identity=27.5766016713092, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788366, Length=355, Percent_Identity=24.7887323943662, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=330, Percent_Identity=36.6666666666667, Blast_Score=205, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI115532424, Length=328, Percent_Identity=32.3170731707317, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17539532, Length=331, Percent_Identity=24.773413897281, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17507723, Length=189, Percent_Identity=26.984126984127, Blast_Score=65, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17539424, Length=189, Percent_Identity=25.9259259259259, Blast_Score=64, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI133901786, Length=189, Percent_Identity=25.9259259259259, Blast_Score=64, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17539422, Length=189, Percent_Identity=25.9259259259259, Blast_Score=64, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI133901788, Length=189, Percent_Identity=25.9259259259259, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6319493, Length=365, Percent_Identity=25.4794520547945, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356223, Length=331, Percent_Identity=26.5861027190332, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39910; Mature: 39779

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILVTGGAGFIGGNFVLDWLAEREETVINLDALTYAGNLETLHTVRTKPNHVFTHGDIG
CEEEEECCCCCCCHHHHHHHHHCCCCEEEEEEHEEECCCCHHHHHCCCCCCEEEECCCCC
DRKLVAELLRTHRPRAIVNFAAESHVDRSIHGPGDFIQTNIVGTFNLLECAREYWSGLEG
CHHHHHHHHHHCCCHHEEHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCC
GDREAFRFLHVSTDEVYGTLRAEDAPFSETNRYEPNSPYSASKAASDHLVRAWHHTYGLP
CCHHHEEEEEEEHHHHHEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
VLTTNCSNNYGPYHFPEKLIPLVILNALAGKPLPIYGDGQQVRDWLYVKDHCSAIRRVLE
EEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHH
AGKLGETYNVGGWNEKTNLEVVNTICGILDQLRPRADGRPYREQIHFVSDRPGHDRRYAI
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHEECCCCCCCCCEEEH
DARKLERELGWKPAETFDSGIRKTIEWYLGNPNWIEHVSNGSYRDWLDKHYG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHCCC
>Mature Secondary Structure 
TILVTGGAGFIGGNFVLDWLAEREETVINLDALTYAGNLETLHTVRTKPNHVFTHGDIG
EEEEECCCCCCCHHHHHHHHHCCCCEEEEEEHEEECCCCHHHHHCCCCCCEEEECCCCC
DRKLVAELLRTHRPRAIVNFAAESHVDRSIHGPGDFIQTNIVGTFNLLECAREYWSGLEG
CHHHHHHHHHHCCCHHEEHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCC
GDREAFRFLHVSTDEVYGTLRAEDAPFSETNRYEPNSPYSASKAASDHLVRAWHHTYGLP
CCHHHEEEEEEEHHHHHEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
VLTTNCSNNYGPYHFPEKLIPLVILNALAGKPLPIYGDGQQVRDWLYVKDHCSAIRRVLE
EEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHH
AGKLGETYNVGGWNEKTNLEVVNTICGILDQLRPRADGRPYREQIHFVSDRPGHDRRYAI
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHEECCCCCCCCCEEEH
DARKLERELGWKPAETFDSGIRKTIEWYLGNPNWIEHVSNGSYRDWLDKHYG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]