Click here to switch to the map view.

The map label for this gene is amtB [H]

Identifier: 34499458

GI number: 34499458

Start: 4329876

End: 4331186

Strand: Direct

Name: amtB [H]

Synonym: CV_4003

Alternate gene names: 34499458

Gene position: 4329876-4331186 (Clockwise)

Preceding gene: 34499457

Following gene: 34499465

Centisome position: 91.13

GC content: 68.88

Gene sequence:

>1311_bases
ATGAAAAAGATACTCGCTGCAATGGCTTCCGGGCTCGCCGCCCTCTCCCTGCCCGCGCTGGCCGATGCGCCGGCCGGCCC
GGCGGTGGCCGCCGCCAAGGTGATCAACTCCGGCGACACCGCCTGGATGCTGACCTCCACCGCCCTGGTGCTGTTCATGA
CCATTCCCGGCCTGGCGCTGTTCTATGGCGGCATGGTCCGCAAGAAGAACGTGCTGGCCACGCTGATGCAGAGCTTCGCC
ATCACCGCGCTGGTGACCGTGCTGTGGGCCGTCGTCGGCTACAGCCTGGCCTTCACCGTCGGCACCCCCTATATCGGCGA
CCTGTCCCGCGTGCTGCTGGACGGCATGGTGTACATGAAGGACGCCGGCAAGCTGGCGGTGCATCCGCTGGCCGGCACCA
TCCCGGAATCGGTGTTCATGACCTTCCAGATGACCTTCGCCATCATCACCCCGGCGCTGATCACCGGCGCCTTCGCCGAG
CGGATGAAGTTCTCTGCGATGCTGGTCTTCATGGCGCTGTGGTCTCTGCTGGTCTATGTGCCGGTCGCCCACTGGGTATG
GGCGCCGGGCGGCTGGATGGGCGACAAGGGCGTGCTGGACTTCGCCGGCGGCACCGTGGTGCACATCAATGCCGGCGTGG
CCGGCCTGGTGACCGCGCTGGTGCTGGGCAAGCGCGTCGGCTTCGGCCGCGAGGCGATGCCGCCCCACAACCTGGTGCTG
ACCCTGGTGGGCGCGTCGATGCTGTGGGTGGGCTGGTTCGGCTTCAACGCCGGCTCGGCGGGCGCCGCCGACGGCCGCGC
CGGCATGGCGATGGTCACCACCCAGGTCGCCACCGCGATGGCCGCGCTGGCCTGGATGTTCGCCGAATGGATCGCCAAGA
AAAAGCCTTCGGTACTGGGCATCGCCTCCGGCGCGGTGGCCGGCCTGGTGGCCATCACCCCGGCCGCCGGCTTCGTCGAC
GTCAAGGGCGCGCTGGCCATCGGCCTGGCCGCCGGCGTGATCTGCTTCTGGGGCGCCACCGGCCTGAAACACATGATGGG
CTACGACGACTCGCTGGACGCTTTCGGCGTGCACGGCGTGGGCGGCATCCTGGGCGCGCTGCTGACCGGCGTGTTCGCGG
TCAAGGACATCGGCGGCGCCGACGGCAGCCTGGCCACCCAGGCGCTGGGCGTGGGCGTGACCATCGTCTACTGCGGCGTG
GTCACCTTCATCCTGCTGAAGCTGGTGGACATCGTGCTCGGCCTGCGCGTCGCGGAGGACGAGGAACGCGAAGGCCTGGA
TCTGGTGCTGCACGGCGAGCGGGTGGAATAA

Upstream 100 bases:

>100_bases
CAAGATATTTGTCTACGACCTGGAGCAGGTGATCCGCATCCGCACCGGCGAGACCGGCGCCGACGCGGTCTGAGCGCTTT
GTGAAACTGGGGAAACCATA

Downstream 100 bases:

>100_bases
GCCGCCGGATCGAAAGATTGTTGTGTAAGGGACTTGGCGGGGCTTCGGCCCCGTTTTTTTTGCGCCTGGCGCATGAAAAA
ACCGGCCAGCGGCCGGTTGG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MKKILAAMASGLAALSLPALADAPAGPAVAAAKVINSGDTAWMLTSTALVLFMTIPGLALFYGGMVRKKNVLATLMQSFA
ITALVTVLWAVVGYSLAFTVGTPYIGDLSRVLLDGMVYMKDAGKLAVHPLAGTIPESVFMTFQMTFAIITPALITGAFAE
RMKFSAMLVFMALWSLLVYVPVAHWVWAPGGWMGDKGVLDFAGGTVVHINAGVAGLVTALVLGKRVGFGREAMPPHNLVL
TLVGASMLWVGWFGFNAGSAGAADGRAGMAMVTTQVATAMAALAWMFAEWIAKKKPSVLGIASGAVAGLVAITPAAGFVD
VKGALAIGLAAGVICFWGATGLKHMMGYDDSLDAFGVHGVGGILGALLTGVFAVKDIGGADGSLATQALGVGVTIVYCGV
VTFILLKLVDIVLGLRVAEDEEREGLDLVLHGERVE

Sequences:

>Translated_436_residues
MKKILAAMASGLAALSLPALADAPAGPAVAAAKVINSGDTAWMLTSTALVLFMTIPGLALFYGGMVRKKNVLATLMQSFA
ITALVTVLWAVVGYSLAFTVGTPYIGDLSRVLLDGMVYMKDAGKLAVHPLAGTIPESVFMTFQMTFAIITPALITGAFAE
RMKFSAMLVFMALWSLLVYVPVAHWVWAPGGWMGDKGVLDFAGGTVVHINAGVAGLVTALVLGKRVGFGREAMPPHNLVL
TLVGASMLWVGWFGFNAGSAGAADGRAGMAMVTTQVATAMAALAWMFAEWIAKKKPSVLGIASGAVAGLVAITPAAGFVD
VKGALAIGLAAGVICFWGATGLKHMMGYDDSLDAFGVHGVGGILGALLTGVFAVKDIGGADGSLATQALGVGVTIVYCGV
VTFILLKLVDIVLGLRVAEDEEREGLDLVLHGERVE
>Mature_436_residues
MKKILAAMASGLAALSLPALADAPAGPAVAAAKVINSGDTAWMLTSTALVLFMTIPGLALFYGGMVRKKNVLATLMQSFA
ITALVTVLWAVVGYSLAFTVGTPYIGDLSRVLLDGMVYMKDAGKLAVHPLAGTIPESVFMTFQMTFAIITPALITGAFAE
RMKFSAMLVFMALWSLLVYVPVAHWVWAPGGWMGDKGVLDFAGGTVVHINAGVAGLVTALVLGKRVGFGREAMPPHNLVL
TLVGASMLWVGWFGFNAGSAGAADGRAGMAMVTTQVATAMAALAWMFAEWIAKKKPSVLGIASGAVAGLVAITPAAGFVD
VKGALAIGLAAGVICFWGATGLKHMMGYDDSLDAFGVHGVGGILGALLTGVFAVKDIGGADGSLATQALGVGVTIVYCGV
VTFILLKLVDIVLGLRVAEDEEREGLDLVLHGERVE

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=439, Percent_Identity=50.3416856492027, Blast_Score=400, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17550182, Length=444, Percent_Identity=27.9279279279279, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17550180, Length=436, Percent_Identity=25.6880733944954, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI32564042, Length=459, Percent_Identity=25.4901960784314, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI133931100, Length=436, Percent_Identity=26.1467889908257, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324187, Length=421, Percent_Identity=36.8171021377672, Blast_Score=234, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6325396, Length=420, Percent_Identity=34.7619047619048, Blast_Score=232, Evalue=8e-62,
Organism=Saccharomyces cerevisiae, GI6321559, Length=412, Percent_Identity=33.7378640776699, Blast_Score=216, Evalue=5e-57,
Organism=Drosophila melanogaster, GI161078314, Length=422, Percent_Identity=28.436018957346, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 44918; Mature: 44918

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILAAMASGLAALSLPALADAPAGPAVAAAKVINSGDTAWMLTSTALVLFMTIPGLAL
CHHHHHHHHHCHHHHCCCHHCCCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHH
FYGGMVRKKNVLATLMQSFAITALVTVLWAVVGYSLAFTVGTPYIGDLSRVLLDGMVYMK
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHEEE
DAGKLAVHPLAGTIPESVFMTFQMTFAIITPALITGAFAERMKFSAMLVFMALWSLLVYV
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PVAHWVWAPGGWMGDKGVLDFAGGTVVHINAGVAGLVTALVLGKRVGFGREAMPPHNLVL
HHHHHHCCCCCCCCCCCCEEECCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHH
TLVGASMLWVGWFGFNAGSAGAADGRAGMAMVTTQVATAMAALAWMFAEWIAKKKPSVLG
HHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IASGAVAGLVAITPAAGFVDVKGALAIGLAAGVICFWGATGLKHMMGYDDSLDAFGVHGV
ECCHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCHH
GGILGALLTGVFAVKDIGGADGSLATQALGVGVTIVYCGVVTFILLKLVDIVLGLRVAED
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EEREGLDLVLHGERVE
CCCCCCEEEEECCCCC
>Mature Secondary Structure
MKKILAAMASGLAALSLPALADAPAGPAVAAAKVINSGDTAWMLTSTALVLFMTIPGLAL
CHHHHHHHHHCHHHHCCCHHCCCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHH
FYGGMVRKKNVLATLMQSFAITALVTVLWAVVGYSLAFTVGTPYIGDLSRVLLDGMVYMK
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHEEE
DAGKLAVHPLAGTIPESVFMTFQMTFAIITPALITGAFAERMKFSAMLVFMALWSLLVYV
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PVAHWVWAPGGWMGDKGVLDFAGGTVVHINAGVAGLVTALVLGKRVGFGREAMPPHNLVL
HHHHHHCCCCCCCCCCCCEEECCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHH
TLVGASMLWVGWFGFNAGSAGAADGRAGMAMVTTQVATAMAALAWMFAEWIAKKKPSVLG
HHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IASGAVAGLVAITPAAGFVDVKGALAIGLAAGVICFWGATGLKHMMGYDDSLDAFGVHGV
ECCHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCHH
GGILGALLTGVFAVKDIGGADGSLATQALGVGVTIVYCGVVTFILLKLVDIVLGLRVAED
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EEREGLDLVLHGERVE
CCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]