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The map label for this gene is glnK [H]

Identifier: 34499457

GI number: 34499457

Start: 4329510

End: 4329848

Strand: Direct

Name: glnK [H]

Synonym: CV_4002

Alternate gene names: 34499457

Gene position: 4329510-4329848 (Clockwise)

Preceding gene: 34499446

Following gene: 34499458

Centisome position: 91.13

GC content: 62.54

Gene sequence:

>339_bases
ATGAAACTCGTTACCGCAGTGATCAAGCCGTTCAAGCTTGACGAAGTCCGCGAAGCGCTGTCCGCCATCGGCGTACAGGG
CATCACCGTCTCCGAGGTCAAGGGCTTCGGCCGCCAGAAAGGCCATACCGAGCTGTATCGCGGCGCCGAGTACGTCGTCG
ACTTCCTGCCCAAGGTGAAGCTGGAGATCGCCATCGACGACGCGCTGCTGGACCAGGTGGTGGAGGCGATTGAGAAATCC
GCCCGCACCGGCAAGATCGGCGACGGCAAGATATTTGTCTACGACCTGGAGCAGGTGATCCGCATCCGCACCGGCGAGAC
CGGCGCCGACGCGGTCTGA

Upstream 100 bases:

>100_bases
AGTGCACCACAATTCCAAGAACGCCCCGCGCGGGCTGGCACGATTCCTGCATAACCATCCGCAGCAGAACCATCTGTCAA
TCGTCCGAGAAGGAGCCGCC

Downstream 100 bases:

>100_bases
GCGCTTTGTGAAACTGGGGAAACCATAATGAAAAAGATACTCGCTGCAATGGCTTCCGGGCTCGCCGCCCTCTCCCTGCC
CGCGCTGGCCGATGCGCCGG

Product: nitrogen regulatory protein P-II-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 112; Mature: 112

Protein sequence:

>112_residues
MKLVTAVIKPFKLDEVREALSAIGVQGITVSEVKGFGRQKGHTELYRGAEYVVDFLPKVKLEIAIDDALLDQVVEAIEKS
ARTGKIGDGKIFVYDLEQVIRIRTGETGADAV

Sequences:

>Translated_112_residues
MKLVTAVIKPFKLDEVREALSAIGVQGITVSEVKGFGRQKGHTELYRGAEYVVDFLPKVKLEIAIDDALLDQVVEAIEKS
ARTGKIGDGKIFVYDLEQVIRIRTGETGADAV
>Mature_112_residues
MKLVTAVIKPFKLDEVREALSAIGVQGITVSEVKGFGRQKGHTELYRGAEYVVDFLPKVKLEIAIDDALLDQVVEAIEKS
ARTGKIGDGKIFVYDLEQVIRIRTGETGADAV

Specific function: P-II indirectly controls the transcription of the glutamine synthetase gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of glnA. When P-II is uridylylated to P-II-UMP, these events are reversed

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1786655, Length=106, Percent_Identity=72.6415094339623, Blast_Score=162, Evalue=6e-42,
Organism=Escherichia coli, GI1788904, Length=106, Percent_Identity=69.811320754717, Blast_Score=158, Evalue=8e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918
- InterPro:   IPR002332 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 12246; Mature: 12246

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00496 PII_GLNB_UMP ; PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVTAVIKPFKLDEVREALSAIGVQGITVSEVKGFGRQKGHTELYRGAEYVVDFLPKVK
CCHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCE
LEIAIDDALLDQVVEAIEKSARTGKIGDGKIFVYDLEQVIRIRTGETGADAV
EEEEECHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKLVTAVIKPFKLDEVREALSAIGVQGITVSEVKGFGRQKGHTELYRGAEYVVDFLPKVK
CCHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCE
LEIAIDDALLDQVVEAIEKSARTGKIGDGKIFVYDLEQVIRIRTGETGADAV
EEEEECHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8704966 [H]