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The map label for this gene is yihU [H]

Identifier: 34499399

GI number: 34499399

Start: 4264233

End: 4265123

Strand: Reverse

Name: yihU [H]

Synonym: CV_3944

Alternate gene names: 34499399

Gene position: 4265123-4264233 (Counterclockwise)

Preceding gene: 34499400

Following gene: 34499398

Centisome position: 89.77

GC content: 73.74

Gene sequence:

>891_bases
ATGAGCAGGCGGAATGCGGGAAAACGGCTGGCGCTGATCGGCTGCGGCGCGATGGGAACCGCGCTGGGACTGCGGCTGCT
GGGCCTGGGCTGGGAGGTGCGGGTCTATAACCGCAGCCCGGAGCGGACGGCGCCGTTGCGGGACGCCGGGGCCAGCGCGC
ACGCGACGCCGGCCGAAGCGGTGGCTGGAGCCGACTGGGTGGCGACGGTGCTGACCGACAGCGCCGCGCTGCGCGCGCTG
TTGTTCGGCGACAACGGCATCGCCAGCGCGCCGGCCGCCGGCCGCCGGCTGGTGGACCTGGGCACCCATCAGCCCACCCA
ATTGGCCGCGCTGGGCGCCGAAGCGGCAGCGGCCGGCTGGCCATTGGTGGAGGCGCCGATGACCGGCAGCGTGCACGACG
CCAGCCACGGCACCCTGCATTTTCTGGTCGGCGGCGCGGAGGCGGACGTGGAGTGGGCGAGCCCGCTGCTGTGGGCGCTG
GGGCGCGGCGTCCATCATCTGGGCGGGCTGGGCGCGGGCAATACCGCCAAGCTGGCTTTGAACCTGCTGGTGGGCGTGAT
GGCGGGCGGGCTGGGCGAGGCGATCGCGTTGCTCAGGGCGCACGGGCTGGCGGTGGACAGCTTCCTCGACGCGCTGGACG
GCAGCGGCCTGGCCTCGCCCTTGTACCGGCGGCTGGGGCAGCGCTATCTGGACGGCGACCACGCGCCGCGCTTCTCGCTG
GCCAATCTGGAGAAAGACCTGCGCTGGGAGTGCGAGCACGCGCTGGCCAGCGGCTTGCAGCCCAGGCTGGCGCCGGCGTT
GTGCCGGCTGCTGGCGCCGCTGAGCGAGGAGGCCAAGCGCCGCGACTACTCGGCCCTGATCGCCGATCTGGCCGGTGCGC
CGTCCTGTTGA

Upstream 100 bases:

>100_bases
TTTCCGACAGATTGCGCCTGGACGAGGTGCCGGATTACTTCGCCGCGCTGTACCGGCGCGAGCGCTTCAAGGCCTTGGTC
GAGGTGGCGGGGGAGGCGGC

Downstream 100 bases:

>100_bases
ATTCATTGTCATGGTTGTTGAATTTATTAAATGATATATTCATTAAAAAACCATGCCGCCTGTCCCTTTCGAAAAACGAG
CGAGCGCTTCATGAGTACAG

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MSRRNAGKRLALIGCGAMGTALGLRLLGLGWEVRVYNRSPERTAPLRDAGASAHATPAEAVAGADWVATVLTDSAALRAL
LFGDNGIASAPAAGRRLVDLGTHQPTQLAALGAEAAAAGWPLVEAPMTGSVHDASHGTLHFLVGGAEADVEWASPLLWAL
GRGVHHLGGLGAGNTAKLALNLLVGVMAGGLGEAIALLRAHGLAVDSFLDALDGSGLASPLYRRLGQRYLDGDHAPRFSL
ANLEKDLRWECEHALASGLQPRLAPALCRLLAPLSEEAKRRDYSALIADLAGAPSC

Sequences:

>Translated_296_residues
MSRRNAGKRLALIGCGAMGTALGLRLLGLGWEVRVYNRSPERTAPLRDAGASAHATPAEAVAGADWVATVLTDSAALRAL
LFGDNGIASAPAAGRRLVDLGTHQPTQLAALGAEAAAAGWPLVEAPMTGSVHDASHGTLHFLVGGAEADVEWASPLLWAL
GRGVHHLGGLGAGNTAKLALNLLVGVMAGGLGEAIALLRAHGLAVDSFLDALDGSGLASPLYRRLGQRYLDGDHAPRFSL
ANLEKDLRWECEHALASGLQPRLAPALCRLLAPLSEEAKRRDYSALIADLAGAPSC
>Mature_295_residues
SRRNAGKRLALIGCGAMGTALGLRLLGLGWEVRVYNRSPERTAPLRDAGASAHATPAEAVAGADWVATVLTDSAALRALL
FGDNGIASAPAAGRRLVDLGTHQPTQLAALGAEAAAAGWPLVEAPMTGSVHDASHGTLHFLVGGAEADVEWASPLLWALG
RGVHHLGGLGAGNTAKLALNLLVGVMAGGLGEAIALLRAHGLAVDSFLDALDGSGLASPLYRRLGQRYLDGDHAPRFSLA
NLEKDLRWECEHALASGLQPRLAPALCRLLAPLSEEAKRRDYSALIADLAGAPSC

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=241, Percent_Identity=28.6307053941909, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI23308751, Length=197, Percent_Identity=31.9796954314721, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1790315, Length=239, Percent_Identity=30.9623430962343, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI145693186, Length=279, Percent_Identity=27.9569892473118, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1786719, Length=283, Percent_Identity=27.5618374558304, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1789092, Length=287, Percent_Identity=23.6933797909408, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17557316, Length=195, Percent_Identity=29.7435897435897, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28574115, Length=242, Percent_Identity=26.4462809917355, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24655230, Length=254, Percent_Identity=28.3464566929134, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19922568, Length=254, Percent_Identity=28.3464566929134, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30533; Mature: 30402

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRNAGKRLALIGCGAMGTALGLRLLGLGWEVRVYNRSPERTAPLRDAGASAHATPAEA
CCCCCCCCEEEEEECCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHCCCCCCCCHHHH
VAGADWVATVLTDSAALRALLFGDNGIASAPAAGRRLVDLGTHQPTQLAALGAEAAAAGW
HHCCHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCEEEECCCCCCHHHHHHCCHHHHCCC
PLVEAPMTGSVHDASHGTLHFLVGGAEADVEWASPLLWALGRGVHHLGGLGAGNTAKLAL
CEEECCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
NLLVGVMAGGLGEAIALLRAHGLAVDSFLDALDGSGLASPLYRRLGQRYLDGDHAPRFSL
HHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCH
ANLEKDLRWECEHALASGLQPRLAPALCRLLAPLSEEAKRRDYSALIADLAGAPSC
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SRRNAGKRLALIGCGAMGTALGLRLLGLGWEVRVYNRSPERTAPLRDAGASAHATPAEA
CCCCCCCEEEEEECCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHCCCCCCCCHHHH
VAGADWVATVLTDSAALRALLFGDNGIASAPAAGRRLVDLGTHQPTQLAALGAEAAAAGW
HHCCHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCEEEECCCCCCHHHHHHCCHHHHCCC
PLVEAPMTGSVHDASHGTLHFLVGGAEADVEWASPLLWALGRGVHHLGGLGAGNTAKLAL
CEEECCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
NLLVGVMAGGLGEAIALLRAHGLAVDSFLDALDGSGLASPLYRRLGQRYLDGDHAPRFSL
HHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCH
ANLEKDLRWECEHALASGLQPRLAPALCRLLAPLSEEAKRRDYSALIADLAGAPSC
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]