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The map label for this gene is galE [H]

Identifier: 34499339

GI number: 34499339

Start: 4191388

End: 4192368

Strand: Reverse

Name: galE [H]

Synonym: CV_3884

Alternate gene names: 34499339

Gene position: 4192368-4191388 (Counterclockwise)

Preceding gene: 34499340

Following gene: 34499338

Centisome position: 88.24

GC content: 67.89

Gene sequence:

>981_bases
CTGCGCGGCGCCTCGCTGTTCATCAGCGGCGGCACCGGCTGGTTCGGCCGCTGGCTGCTGGAGGCCATCGCCCACGCCAA
CGCCCGGCTGGGAACGGATATCCGCGTGCTGGCGCTCAGCCGCGATCCGGGCGCCTTCGCGCGCCGCGCGCCGCACCTGG
CGTCCAATCCCGCCATCCGCTTCCACATCGGCGACGTCCGCGACTTCGACTTTCCCCGCGAGCGCTTCACCCATGTCCTC
CACGCGGCCACCACGTCGGCGCATGAAACCTTCAACGGCGAAAGTCCGCTGGGCAAGTTCGACACCCTAGTGAGCGGTAC
CCGGCGCGTGCTGGATTTCGCGGCGGAGGCCGGTGTCCGGCATTTCCTGTTCACCAGCTCCGGCGCGGCCTACGGTCCGT
CGCCGCGCGGCCTGCCGTTGCGGGAGGACGACGTCTGCGCGCCGGACACCATGGACCCGATGACGGCCTTGGGGCAGGCC
AAGCGCGCGGCGGAGTTCCTGTGCGCCGCTTATGCCCGGCAATATGGCTGGCATCTGAGCGCCGCCCGCTGTTTCGCCTT
CGTCGGGCCTTTCATGCCGCTGGACATCCATTATGCGATCGGCAATTTCATCCAGTGCGCGCTGCAAGGAGAGCCTATCA
TCATCAATGGAGATGGCTCTCCGGTCCGTTCCTATCTGTACATGGGCGACTTGGTGGTCTGGCTGATGACGCTGCTGCAA
CGCGAGGGGGCGGCCGAGGTCTTCAACGTCGGATCGGATGAGGCCATCGACATCGCGGCGCTGGCGAACCGGGTCCGGGA
CCGGCTGAATCCGGGCGTGGAGGTGCGCATCCTGGGCCGCTCCGACGCCAGCGTCGGCAACCCGGTCCGCAACCTGTACC
TGCCCGACATCGGCAGGGCGCGGGAGAGGCTGGGCCTGGAGCCCTGGACTTCGCTGGACGATGCCATCCGGTTTTCGGCC
GACGCGTTTCAACAGGCATGA

Upstream 100 bases:

>100_bases
CGGCCGCATGCCCGCAGACCGCCGCGCCGCGGAGCCGCGCCGCGCGCTGAACGACGATCTGGACGCCATCCTCGCGCGCA
AGCCCGAGGTGTGGGAGAGC

Downstream 100 bases:

>100_bases
TATTGTCTGCTAGCCGGCTTGGCCGGTTGGCGTTCTTGTTCCGGGCGGGCCGAGGCGGCAGGCTGGAGCAAGGGATACAC
GAAAGCAAGCGAACATGCAG

Product: UDP-glucose 4-epimerase

Products: UDPgalactose

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MRGASLFISGGTGWFGRWLLEAIAHANARLGTDIRVLALSRDPGAFARRAPHLASNPAIRFHIGDVRDFDFPRERFTHVL
HAATTSAHETFNGESPLGKFDTLVSGTRRVLDFAAEAGVRHFLFTSSGAAYGPSPRGLPLREDDVCAPDTMDPMTALGQA
KRAAEFLCAAYARQYGWHLSAARCFAFVGPFMPLDIHYAIGNFIQCALQGEPIIINGDGSPVRSYLYMGDLVVWLMTLLQ
REGAAEVFNVGSDEAIDIAALANRVRDRLNPGVEVRILGRSDASVGNPVRNLYLPDIGRARERLGLEPWTSLDDAIRFSA
DAFQQA

Sequences:

>Translated_326_residues
MRGASLFISGGTGWFGRWLLEAIAHANARLGTDIRVLALSRDPGAFARRAPHLASNPAIRFHIGDVRDFDFPRERFTHVL
HAATTSAHETFNGESPLGKFDTLVSGTRRVLDFAAEAGVRHFLFTSSGAAYGPSPRGLPLREDDVCAPDTMDPMTALGQA
KRAAEFLCAAYARQYGWHLSAARCFAFVGPFMPLDIHYAIGNFIQCALQGEPIIINGDGSPVRSYLYMGDLVVWLMTLLQ
REGAAEVFNVGSDEAIDIAALANRVRDRLNPGVEVRILGRSDASVGNPVRNLYLPDIGRARERLGLEPWTSLDDAIRFSA
DAFQQA
>Mature_326_residues
MRGASLFISGGTGWFGRWLLEAIAHANARLGTDIRVLALSRDPGAFARRAPHLASNPAIRFHIGDVRDFDFPRERFTHVL
HAATTSAHETFNGESPLGKFDTLVSGTRRVLDFAAEAGVRHFLFTSSGAAYGPSPRGLPLREDDVCAPDTMDPMTALGQA
KRAAEFLCAAYARQYGWHLSAARCFAFVGPFMPLDIHYAIGNFIQCALQGEPIIINGDGSPVRSYLYMGDLVVWLMTLLQ
REGAAEVFNVGSDEAIDIAALANRVRDRLNPGVEVRILGRSDASVGNPVRNLYLPDIGRARERLGLEPWTSLDDAIRFSA
DAFQQA

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=231, Percent_Identity=30.7359307359307, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17539532, Length=319, Percent_Identity=27.5862068965517, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21356223, Length=249, Percent_Identity=28.5140562248996, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2

Molecular weight: Translated: 35630; Mature: 35630

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGASLFISGGTGWFGRWLLEAIAHANARLGTDIRVLALSRDPGAFARRAPHLASNPAIR
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHCCCCCCCCCEEE
FHIGDVRDFDFPRERFTHVLHAATTSAHETFNGESPLGKFDTLVSGTRRVLDFAAEAGVR
EEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCE
HFLFTSSGAAYGPSPRGLPLREDDVCAPDTMDPMTALGQAKRAAEFLCAAYARQYGWHLS
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
AARCFAFVGPFMPLDIHYAIGNFIQCALQGEPIIINGDGSPVRSYLYMGDLVVWLMTLLQ
HHHHHHHHCCCCCEEHHHHHHHHHHHEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
REGAAEVFNVGSDEAIDIAALANRVRDRLNPGVEVRILGRSDASVGNPVRNLYLPDIGRA
CCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHCCCCCHHHH
RERLGLEPWTSLDDAIRFSADAFQQA
HHHCCCCCCCCHHHHHHHCHHHHHCC
>Mature Secondary Structure
MRGASLFISGGTGWFGRWLLEAIAHANARLGTDIRVLALSRDPGAFARRAPHLASNPAIR
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHCCCCCCCCCEEE
FHIGDVRDFDFPRERFTHVLHAATTSAHETFNGESPLGKFDTLVSGTRRVLDFAAEAGVR
EEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCE
HFLFTSSGAAYGPSPRGLPLREDDVCAPDTMDPMTALGQAKRAAEFLCAAYARQYGWHLS
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
AARCFAFVGPFMPLDIHYAIGNFIQCALQGEPIIINGDGSPVRSYLYMGDLVVWLMTLLQ
HHHHHHHHCCCCCEEHHHHHHHHHHHEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
REGAAEVFNVGSDEAIDIAALANRVRDRLNPGVEVRILGRSDASVGNPVRNLYLPDIGRA
CCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHCCCCCHHHH
RERLGLEPWTSLDDAIRFSADAFQQA
HHHCCCCCCCCHHHHHHHCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDP-glucose

Specific reaction: UDP-glucose = UDP-galactose

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]