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The map label for this gene is rstA [H]

Identifier: 34499298

GI number: 34499298

Start: 4148075

End: 4148788

Strand: Direct

Name: rstA [H]

Synonym: CV_3843

Alternate gene names: 34499298

Gene position: 4148075-4148788 (Clockwise)

Preceding gene: 34499297

Following gene: 34499299

Centisome position: 87.31

GC content: 66.25

Gene sequence:

>714_bases
ATGACCCATCGCATCGTATTCGTCGAAGACGACGCCGACCTCGCCGAACTGATCAGCGATTTCCTGTCCCGCCACGAAAT
GGAAGTGGTGATCGAACCGCGCGGCGACACCGCGCTGGACACCATCGCCCGCGAAGCGCCGGACCTGGTGCTGCTGGACA
TCATGCTGCCGGGCAAGGACGGCCTGTCCATCTGCCGCGAACTGCGGCCCAAGTTCGACGGGCCCATCATCATGCTGACC
TCGCTGGACAGCGACATGAACCAGATCCTGGGCCTGGAGCTCGGCGCCAACGACTACATCCTGAAAACCACGCCGCCGTC
GGTGCTAGTGGCCAGGCTGCGCGCCCAGCTGCGCAACCTGCGGCCGGCCCAGGCCGCCGAGGCGCTGCCGGCCAAGGGCT
CGCGCAAGGCGGTGTTCGGCCAGCTGTCGATCGATCCGGTCAGCCGCGACGTGGTGCTCTGCGGCGAAAAGATCCCGCTG
TCCACCTCGGACTTCGACCTGCTGTGGCTGCTGGCCAGCCACGCCGGCGAGACGCTGAACCGCGACCTGCTGCTGAAGGA
AATGCGCGGCGTCGACTACGACGGCCTGGACCGCAGCATAGACGTGGCCATCTCCCGCCTGCGCAAGAAACTGGGCGACA
ACCCCAGCGAGCCGTTCCGCATCAAGACTGTGCGCAACCGCGGCTACCTGTTCTCGCTGTCGGGCTGGGAGTAA

Upstream 100 bases:

>100_bases
TGCCCGGTCCGGCCTGTCCGAGACGGCCCCGCACTTGAGCGGCGGGGCCGGGCGCGCGATACTTGCCGGCGAACCGCCAA
TCAACCGCATGGATACCCGC

Downstream 100 bases:

>100_bases
GCGCGGATGCGCCGTCTTTTCGTCCAGTTCTACCTGCTGCTGATGGCCTGCTTCCTGGTGGCCGTCATCATGGTCGGCCT
GGTGTACAAGCAAGCGTCGA

Product: DNA-binding transcriptional regulator RstA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MTHRIVFVEDDADLAELISDFLSRHEMEVVIEPRGDTALDTIAREAPDLVLLDIMLPGKDGLSICRELRPKFDGPIIMLT
SLDSDMNQILGLELGANDYILKTTPPSVLVARLRAQLRNLRPAQAAEALPAKGSRKAVFGQLSIDPVSRDVVLCGEKIPL
STSDFDLLWLLASHAGETLNRDLLLKEMRGVDYDGLDRSIDVAISRLRKKLGDNPSEPFRIKTVRNRGYLFSLSGWE

Sequences:

>Translated_237_residues
MTHRIVFVEDDADLAELISDFLSRHEMEVVIEPRGDTALDTIAREAPDLVLLDIMLPGKDGLSICRELRPKFDGPIIMLT
SLDSDMNQILGLELGANDYILKTTPPSVLVARLRAQLRNLRPAQAAEALPAKGSRKAVFGQLSIDPVSRDVVLCGEKIPL
STSDFDLLWLLASHAGETLNRDLLLKEMRGVDYDGLDRSIDVAISRLRKKLGDNPSEPFRIKTVRNRGYLFSLSGWE
>Mature_236_residues
THRIVFVEDDADLAELISDFLSRHEMEVVIEPRGDTALDTIAREAPDLVLLDIMLPGKDGLSICRELRPKFDGPIIMLTS
LDSDMNQILGLELGANDYILKTTPPSVLVARLRAQLRNLRPAQAAEALPAKGSRKAVFGQLSIDPVSRDVVLCGEKIPLS
TSDFDLLWLLASHAGETLNRDLLLKEMRGVDYDGLDRSIDVAISRLRKKLGDNPSEPFRIKTVRNRGYLFSLSGWE

Specific function: Member of the two-component regulatory system rstB/rstA [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI145693140, Length=238, Percent_Identity=63.0252100840336, Blast_Score=290, Evalue=6e-80,
Organism=Escherichia coli, GI1789809, Length=235, Percent_Identity=38.2978723404255, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1786599, Length=235, Percent_Identity=34.0425531914894, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1788394, Length=229, Percent_Identity=35.8078602620087, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI87082012, Length=228, Percent_Identity=35.9649122807018, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=36.2445414847162, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1786784, Length=231, Percent_Identity=34.6320346320346, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1790863, Length=232, Percent_Identity=32.3275862068966, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1789402, Length=227, Percent_Identity=31.7180616740088, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787229, Length=213, Percent_Identity=29.5774647887324, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790860, Length=234, Percent_Identity=28.6324786324786, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1790552, Length=184, Percent_Identity=32.0652173913043, Blast_Score=87, Evalue=7e-19,
Organism=Escherichia coli, GI1786911, Length=229, Percent_Identity=28.3842794759825, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787375, Length=242, Percent_Identity=30.1652892561983, Blast_Score=80, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26362; Mature: 26231

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHRIVFVEDDADLAELISDFLSRHEMEVVIEPRGDTALDTIAREAPDLVLLDIMLPGKD
CCEEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCEEEEEEEECCCC
GLSICRELRPKFDGPIIMLTSLDSDMNQILGLELGANDYILKTTPPSVLVARLRAQLRNL
HHHHHHHHCCCCCCCEEEEEECCCHHHHHHEEEECCCCEEEECCCHHHHHHHHHHHHHCC
RPAQAAEALPAKGSRKAVFGQLSIDPVSRDVVLCGEKIPLSTSDFDLLWLLASHAGETLN
CHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCHHH
RDLLLKEMRGVDYDGLDRSIDVAISRLRKKLGDNPSEPFRIKTVRNRGYLFSLSGWE
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCC
>Mature Secondary Structure 
THRIVFVEDDADLAELISDFLSRHEMEVVIEPRGDTALDTIAREAPDLVLLDIMLPGKD
CEEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCEEEEEEEECCCC
GLSICRELRPKFDGPIIMLTSLDSDMNQILGLELGANDYILKTTPPSVLVARLRAQLRNL
HHHHHHHHCCCCCCCEEEEEECCCHHHHHHEEEECCCCEEEECCCHHHHHHHHHHHHHCC
RPAQAAEALPAKGSRKAVFGQLSIDPVSRDVVLCGEKIPLSTSDFDLLWLLASHAGETLN
CHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCHHH
RDLLLKEMRGVDYDGLDRSIDVAISRLRKKLGDNPSEPFRIKTVRNRGYLFSLSGWE
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1495392 [H]