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The map label for this gene is rstB [H]

Identifier: 34499299

GI number: 34499299

Start: 4148795

End: 4150069

Strand: Direct

Name: rstB [H]

Synonym: CV_3844

Alternate gene names: 34499299

Gene position: 4148795-4150069 (Clockwise)

Preceding gene: 34499298

Following gene: 34499301

Centisome position: 87.32

GC content: 67.14

Gene sequence:

>1275_bases
ATGCGCCGTCTTTTCGTCCAGTTCTACCTGCTGCTGATGGCCTGCTTCCTGGTGGCCGTCATCATGGTCGGCCTGGTGTA
CAAGCAAGCGTCGAACGAGAAGGGCGAACGCTACCTGTCCGACCTCCTGCGCACCACGCTGTCGCTGATCGAGGCCGATC
TGCGCGGCGTGCCGGAGGACCGCTGGAGCGAGACCCTGGCCAACTCCGACTACGGCTTCACCTTCCGCGTGAAGGTGGAG
AAGATGAGCAACTACATCCTGGACGAGGAATCCAAGCAGGCGCTGGACCGCGGCGAAATCGTGATGCTGGAGGACAAGTA
CCTGTTCCTGCAGAAGCTGCAGGACAGCAACTACCTGCTGGTGGCCGGACCGCTGCGCTACCTGTTCTTCCTGCACCAGC
TCAAGTGGGTGGACTACGCGCTGCTCGCCCTGCTGGGACTGTCGCTGGCGATCCCGGTGTTCCTGTGGATGCGGCCGCAC
TGGCGCGACCTGGTGGCGCTGGAGCGCACCGCCGGCCGGCTGGCCAGCGGCGACCTGGCCGCCCGCGTCGACCTGCCGCC
GCATTCCGGCGTCCACCACCTGGGGATCGCCTTCAACCACATGGCCGACAACATCGGCGCGCTGATCAACAGCAAGAAAA
CGCTGACCAACGCCGTCGCCCACGAGCTGCGCACGCCGCTGGCGCGGCTGCGCTACCGGCTGGCCCTGCTGGAGGGCGAC
GAGGATACGCCGGAGCGCCAAGCCATAGAGCGCGACCTCACCGCCATCGACAAGCTGGTGGAAGAATTGCTGTTCCATGC
CAGGCTGGACCGTCCCGAGGCGCCGCTGAAGCCGGTCAGCTTCAACGCCCTGCCCTGGGCGCGCGACCGCATCGCCGGCC
AAGCCGCGCTGGCGCAGGAGATCCACTGGATCGAGCCCGCGGGCGAGGAGCTGACCATCACCGCCGACGAGCACCTGATC
ACCCGCGCGCTGGACAATCTGCTGTCCAACGCCCGCCGCCACGCCAACGGCGTCGTCGCCACCCATATCCTGGTCGACAA
GCAACAATACTGCATCATCGTCGACGACGACGGCCCCGGCATCCCGGAAGAGGACCGCGAACGGGTGTTCGACCCCTTCG
TCCGCCTGGACGAAAGCCGCGGCCGCAAGACCGGCGGCCATGGCTTGGGACTGGCCATCGTCGCCGGCATCGCCCGCGCC
CACCGCGGCAGCATCAAGGTGGAAAGCTCGCCCATGGGCGGCGCGCGCTTCGTGCTGCGTTGGCCTACAGGCTGA

Upstream 100 bases:

>100_bases
GCGCAAGAAACTGGGCGACAACCCCAGCGAGCCGTTCCGCATCAAGACTGTGCGCAACCGCGGCTACCTGTTCTCGCTGT
CGGGCTGGGAGTAAGCGCGG

Downstream 100 bases:

>100_bases
TGTACATTTTGTAACATTGAGATACAAGCCTCCCCTAGACGCTCCCCCTTGCCACCGATAGCATTCGCCCTGAATTAGGA
CTTCTTCGGGTCCGTTTCAA

Product: sensor protein RstB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MRRLFVQFYLLLMACFLVAVIMVGLVYKQASNEKGERYLSDLLRTTLSLIEADLRGVPEDRWSETLANSDYGFTFRVKVE
KMSNYILDEESKQALDRGEIVMLEDKYLFLQKLQDSNYLLVAGPLRYLFFLHQLKWVDYALLALLGLSLAIPVFLWMRPH
WRDLVALERTAGRLASGDLAARVDLPPHSGVHHLGIAFNHMADNIGALINSKKTLTNAVAHELRTPLARLRYRLALLEGD
EDTPERQAIERDLTAIDKLVEELLFHARLDRPEAPLKPVSFNALPWARDRIAGQAALAQEIHWIEPAGEELTITADEHLI
TRALDNLLSNARRHANGVVATHILVDKQQYCIIVDDDGPGIPEEDRERVFDPFVRLDESRGRKTGGHGLGLAIVAGIARA
HRGSIKVESSPMGGARFVLRWPTG

Sequences:

>Translated_424_residues
MRRLFVQFYLLLMACFLVAVIMVGLVYKQASNEKGERYLSDLLRTTLSLIEADLRGVPEDRWSETLANSDYGFTFRVKVE
KMSNYILDEESKQALDRGEIVMLEDKYLFLQKLQDSNYLLVAGPLRYLFFLHQLKWVDYALLALLGLSLAIPVFLWMRPH
WRDLVALERTAGRLASGDLAARVDLPPHSGVHHLGIAFNHMADNIGALINSKKTLTNAVAHELRTPLARLRYRLALLEGD
EDTPERQAIERDLTAIDKLVEELLFHARLDRPEAPLKPVSFNALPWARDRIAGQAALAQEIHWIEPAGEELTITADEHLI
TRALDNLLSNARRHANGVVATHILVDKQQYCIIVDDDGPGIPEEDRERVFDPFVRLDESRGRKTGGHGLGLAIVAGIARA
HRGSIKVESSPMGGARFVLRWPTG
>Mature_424_residues
MRRLFVQFYLLLMACFLVAVIMVGLVYKQASNEKGERYLSDLLRTTLSLIEADLRGVPEDRWSETLANSDYGFTFRVKVE
KMSNYILDEESKQALDRGEIVMLEDKYLFLQKLQDSNYLLVAGPLRYLFFLHQLKWVDYALLALLGLSLAIPVFLWMRPH
WRDLVALERTAGRLASGDLAARVDLPPHSGVHHLGIAFNHMADNIGALINSKKTLTNAVAHELRTPLARLRYRLALLEGD
EDTPERQAIERDLTAIDKLVEELLFHARLDRPEAPLKPVSFNALPWARDRIAGQAALAQEIHWIEPAGEELTITADEHLI
TRALDNLLSNARRHANGVVATHILVDKQQYCIIVDDDGPGIPEEDRERVFDPFVRLDESRGRKTGGHGLGLAIVAGIARA
HRGSIKVESSPMGGARFVLRWPTG

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=424, Percent_Identity=46.9339622641509, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1790346, Length=272, Percent_Identity=34.1911764705882, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI1789808, Length=263, Percent_Identity=31.9391634980989, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1786783, Length=281, Percent_Identity=30.9608540925267, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788393, Length=262, Percent_Identity=26.7175572519084, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI145693157, Length=245, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788549, Length=218, Percent_Identity=32.5688073394495, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786600, Length=221, Percent_Identity=29.4117647058824, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790861, Length=210, Percent_Identity=32.8571428571429, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI87081816, Length=232, Percent_Identity=30.1724137931034, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790551, Length=221, Percent_Identity=28.0542986425339, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786912, Length=229, Percent_Identity=30.5676855895196, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787374, Length=299, Percent_Identity=26.4214046822742, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789149, Length=221, Percent_Identity=28.9592760180996, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790436, Length=217, Percent_Identity=29.4930875576037, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788279, Length=219, Percent_Identity=31.0502283105023, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789403, Length=254, Percent_Identity=25.9842519685039, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI87082128, Length=284, Percent_Identity=25, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47787; Mature: 47787

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLFVQFYLLLMACFLVAVIMVGLVYKQASNEKGERYLSDLLRTTLSLIEADLRGVPED
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
RWSETLANSDYGFTFRVKVEKMSNYILDEESKQALDRGEIVMLEDKYLFLQKLQDSNYLL
HHHHHHCCCCCCEEEEEEHHHHHHHHCCCHHHHHHCCCCEEEEECHHHHHHHHCCCCEEE
VAGPLRYLFFLHQLKWVDYALLALLGLSLAIPVFLWMRPHWRDLVALERTAGRLASGDLA
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCE
ARVDLPPHSGVHHLGIAFNHMADNIGALINSKKTLTNAVAHELRTPLARLRYRLALLEGD
EEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEECC
EDTPERQAIERDLTAIDKLVEELLFHARLDRPEAPLKPVSFNALPWARDRIAGQAALAQE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
IHWIEPAGEELTITADEHLITRALDNLLSNARRHANGVVATHILVDKQQYCIIVDDDGPG
HHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEECCCCC
IPEEDRERVFDPFVRLDESRGRKTGGHGLGLAIVAGIARAHRGSIKVESSPMGGARFVLR
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEE
WPTG
CCCC
>Mature Secondary Structure
MRRLFVQFYLLLMACFLVAVIMVGLVYKQASNEKGERYLSDLLRTTLSLIEADLRGVPED
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
RWSETLANSDYGFTFRVKVEKMSNYILDEESKQALDRGEIVMLEDKYLFLQKLQDSNYLL
HHHHHHCCCCCCEEEEEEHHHHHHHHCCCHHHHHHCCCCEEEEECHHHHHHHHCCCCEEE
VAGPLRYLFFLHQLKWVDYALLALLGLSLAIPVFLWMRPHWRDLVALERTAGRLASGDLA
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCE
ARVDLPPHSGVHHLGIAFNHMADNIGALINSKKTLTNAVAHELRTPLARLRYRLALLEGD
EEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEECC
EDTPERQAIERDLTAIDKLVEELLFHARLDRPEAPLKPVSFNALPWARDRIAGQAALAQE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
IHWIEPAGEELTITADEHLITRALDNLLSNARRHANGVVATHILVDKQQYCIIVDDDGPG
HHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEECCCCC
IPEEDRERVFDPFVRLDESRGRKTGGHGLGLAIVAGIARAHRGSIKVESSPMGGARFVLR
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEE
WPTG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]