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The map label for this gene is pilD [H]

Identifier: 34499281

GI number: 34499281

Start: 4123511

End: 4124428

Strand: Reverse

Name: pilD [H]

Synonym: CV_3826

Alternate gene names: 34499281

Gene position: 4124428-4123511 (Counterclockwise)

Preceding gene: 34499282

Following gene: 34499280

Centisome position: 86.81

GC content: 66.12

Gene sequence:

>918_bases
ATGCTGGATGACATGACATGGCTGCTGGCCACTCACCCCGCTTGGCTGGCAGGCGCGGCGCTTGTGTTCGGATTGATGGT
GGGGAGTTTTCTGAACGTGGTGATCCACAGGCTGCCCAGGATGCTGGAGCGGGAGTTCCTGGCCGACAGCGTGGAGTACC
TGGCGGAAGGGGGCGCGCCTGCCGCGCTGCGCTTGGCCGCGGAGCAGGCCAGGCACGAGCTGGACGATGGCGGCTACAAT
CTGTGGCGGCCCGCCTCGCATTGCCCCGCTTGCCGCGCGCCGGTGCGGCCCTGGCACAATGTGCCCTTGCTCAGTTACCT
GCTGCTGCACGGCCGCTGCGGCGATTGCGGCGAAGCTATCAGCCGGCGATATCCGCTGGTGGAGCTGCTGTGCGGCGCAC
TGTACGGTTTCCTGGCGTGGAAGCTGGGCTGGGGCTGGCCGCTGGCCGGCGCGCTGGCGCTGACGGCTGTCCTGCTGGCG
CTGGCCTTCATCGATTTCGACACCAAGCTGCTGCCGGATGGCCTGACCCTGCCGCTGCTGTGGGGCGGCTTGCTGTTCAA
CCTGATGACGGGGCTGGTGCCGCTGTCGGCCGCGGTGTTGGGGGCGGTTTATGGTTACATGACGCTATGGCTGGTGTCGC
ATGCCTACAAGCTGGTGGCGGGACGGCATGGCATGGGGGGCGGTGATTTCAAGCTGCTGGCGGCGCTGGGCGCCTGGCTG
GGATGGACCATGCTGCCGCTGATCATATTGTTGTCGTCTTTGGTTGGAGCGGTTTGCGGCATCGCCATGATGTCCGCCTC
GCGCGTTGGCCGGGGGCAGGAAATCCCGTTCGGCCCCTATCTTTCGGCCGCCGGCTGGATCGCGCTGGTGTGGGGGCCGC
AGATCATGAATGGATATTTGACATGGCTATCCCGGTAG

Upstream 100 bases:

>100_bases
TGCTGGAGCCGGCGATCATGGTGATTCTGGGGATATTGATAGGCGGGTTGGTGATCGCGATGTACATGCCGATTTTCAAG
ATGGGCCAGGTGGTGGGTTG

Downstream 100 bases:

>100_bases
TGGGGCTGACCGGCGGCATCGGCTCAGGCAAGAGCGCGGCGGCGGACCGCTTCGCCGAGCTGGGCGTGCCGGTCATCGAT
ACCGACAGCATCGCCCACCA

Product: type IV prepilin-like proteins leader peptide processing enzyme

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MLDDMTWLLATHPAWLAGAALVFGLMVGSFLNVVIHRLPRMLEREFLADSVEYLAEGGAPAALRLAAEQARHELDDGGYN
LWRPASHCPACRAPVRPWHNVPLLSYLLLHGRCGDCGEAISRRYPLVELLCGALYGFLAWKLGWGWPLAGALALTAVLLA
LAFIDFDTKLLPDGLTLPLLWGGLLFNLMTGLVPLSAAVLGAVYGYMTLWLVSHAYKLVAGRHGMGGGDFKLLAALGAWL
GWTMLPLIILLSSLVGAVCGIAMMSASRVGRGQEIPFGPYLSAAGWIALVWGPQIMNGYLTWLSR

Sequences:

>Translated_305_residues
MLDDMTWLLATHPAWLAGAALVFGLMVGSFLNVVIHRLPRMLEREFLADSVEYLAEGGAPAALRLAAEQARHELDDGGYN
LWRPASHCPACRAPVRPWHNVPLLSYLLLHGRCGDCGEAISRRYPLVELLCGALYGFLAWKLGWGWPLAGALALTAVLLA
LAFIDFDTKLLPDGLTLPLLWGGLLFNLMTGLVPLSAAVLGAVYGYMTLWLVSHAYKLVAGRHGMGGGDFKLLAALGAWL
GWTMLPLIILLSSLVGAVCGIAMMSASRVGRGQEIPFGPYLSAAGWIALVWGPQIMNGYLTWLSR
>Mature_305_residues
MLDDMTWLLATHPAWLAGAALVFGLMVGSFLNVVIHRLPRMLEREFLADSVEYLAEGGAPAALRLAAEQARHELDDGGYN
LWRPASHCPACRAPVRPWHNVPLLSYLLLHGRCGDCGEAISRRYPLVELLCGALYGFLAWKLGWGWPLAGALALTAVLLA
LAFIDFDTKLLPDGLTLPLLWGGLLFNLMTGLVPLSAAVLGAVYGYMTLWLVSHAYKLVAGRHGMGGGDFKLLAALGAWL
GWTMLPLIILLSSLVGAVCGIAMMSASRVGRGQEIPFGPYLSAAGWIALVWGPQIMNGYLTWLSR

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=281, Percent_Identity=35.2313167259786, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1789732, Length=159, Percent_Identity=50.314465408805, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 32975; Mature: 32975

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDDMTWLLATHPAWLAGAALVFGLMVGSFLNVVIHRLPRMLEREFLADSVEYLAEGGAP
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AALRLAAEQARHELDDGGYNLWRPASHCPACRAPVRPWHNVPLLSYLLLHGRCGDCGEAI
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHH
SRRYPLVELLCGALYGFLAWKLGWGWPLAGALALTAVLLALAFIDFDTKLLPDGLTLPLL
HCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHH
WGGLLFNLMTGLVPLSAAVLGAVYGYMTLWLVSHAYKLVAGRHGMGGGDFKLLAALGAWL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
GWTMLPLIILLSSLVGAVCGIAMMSASRVGRGQEIPFGPYLSAAGWIALVWGPQIMNGYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCEEEEECHHHHHHHH
TWLSR
HHHCC
>Mature Secondary Structure
MLDDMTWLLATHPAWLAGAALVFGLMVGSFLNVVIHRLPRMLEREFLADSVEYLAEGGAP
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AALRLAAEQARHELDDGGYNLWRPASHCPACRAPVRPWHNVPLLSYLLLHGRCGDCGEAI
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHH
SRRYPLVELLCGALYGFLAWKLGWGWPLAGALALTAVLLALAFIDFDTKLLPDGLTLPLL
HCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHH
WGGLLFNLMTGLVPLSAAVLGAVYGYMTLWLVSHAYKLVAGRHGMGGGDFKLLAALGAWL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
GWTMLPLIILLSSLVGAVCGIAMMSASRVGRGQEIPFGPYLSAAGWIALVWGPQIMNGYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCEEEEECHHHHHHHH
TWLSR
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8100347; 7906688; 7565116 [H]