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The map label for this gene is dctA

Identifier: 34499162

GI number: 34499162

Start: 4008244

End: 4009569

Strand: Direct

Name: dctA

Synonym: CV_3707

Alternate gene names: 34499162

Gene position: 4008244-4009569 (Clockwise)

Preceding gene: 34499161

Following gene: 34499163

Centisome position: 84.36

GC content: 63.73

Gene sequence:

>1326_bases
ATGCAAACCAAGACTCCGTTCTTTTCGCGCCTGTACGTCCAGGTGCTGATCGCCATCGCGCTGGGCGTCACGCTCGGCGC
TCTCGCGCCTGACCTCGGCGCCAAGATGAAACCGCTGGGCGACGCCTTCATCAAGCTGATCAAGATGATGATCGCGCCCA
TCATCTTCGCCACCGTCGTGGTCGGCATCGCCAAGATGGGCGACATGAAGGAAGTGGGCCGCGTCGGCGTCAAGGCGCTG
TTCTACTTCGAAGCCGTCACCACGCTGGCGCTGGTGATCGGCCTGATCGTGGTCAACCTGCTGCAGCCGGGCGCCGGCCT
CAACATCGACCCGCATACGCTACACGCCAAGGACATCGCCAAGTTCACCGCCGGCGCCAAGGAAATGAACACGGTCGATT
TCCTGCTGCACATCATCCCGGACACGGTGGTCGGCGCCTTCGCCAGCGGCGAGATCCTGCAGGTGCTGACCTTCTCGGTG
CTGTTGGGCCTGGCGCTGACCAAGATGGGCGACAACGGCAAGACCATCGTCCACATCCTGGACGAGTTCTCGCACGCGCT
GTTCGGCGTGATCAACATGCTGATGAAGCTGGCCCCGATCGGCGCCTTCGGCGCGATGGCCTTCACCATCGGCAAATACG
GCATCGGTTCGCTGAAGCAGCTGGGCTTTTTGATGGCCTGCGTGTATCTCACCTGCTTCGCCTTCGTGTTCATCGTGCTG
GGCGCCATCGCCCGCTTCTCCGGCTTCAGCCTGTGGCGCTTTTTGGTCTACATCAAGGAAGAGATCCTGCTGGTGCTCGG
CACCTCTTCTTCCGAATCCGCGCTGCCCGGCATCATGAAGAAACTGGAAAACCTGGGCTGCTCCAAGCCGGTGGTCGGCA
TGGTGGTGCCCACCGGCTACTCGTTCAACCTGGACGGCACTTCCATCTACCTGACCATGGCGGCGATCTTCATCGCCCAG
GCCACCAATGTGAACCTGACGCTGGGCGAGGAGCTGGCTATCATCGGCGTGCTGCTGCTGACCTCCAAGGGCGCGGCGGC
GGTGACCGGCGGCGGCTTCATCACGCTGGCCGCCACGCTGGCGACGCTGGGCGGCAAGCTGCCGGTGGAGGGCCTGGCGC
TGCTGATCGGCATCGACCGCTTCATGTCCGAGGCGCGCGCCATCACCAACCTGATCGGCAACGGCGTCGCCACCGTGGTG
ATCGCCAAGTGGGAAAAGGCGCTGGACGTCGAACGGATGCGCCAGGTGCTGGAAGGAAGCGAGCCCCCGGCTCCGCCCAA
GACCAAGCGCGAGACCATGCCGGAACCGGCGCTGCAGACTCGCTGA

Upstream 100 bases:

>100_bases
ATATCGACAATGCTCCCGGCTGAAGGCTGTTGTTAGCATCGCTGGAAAATGGACCGTTCAAGAGTCCGGATTCCTGCAAT
CAACGCCAGTCGGGAGAACC

Downstream 100 bases:

>100_bases
GCCTCCCACCCCGCAATACGTACCCAACCCGCTGCGAAGCGGGTTTTTTCGTTTTATTTAGGACGAAATTCTTGATTTCG
CCGTTCGCTCGTCCACGATA

Product: C4-dicarboxylic acids transport protein (Na+/H+ dicarboxylate symporter)

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MQTKTPFFSRLYVQVLIAIALGVTLGALAPDLGAKMKPLGDAFIKLIKMMIAPIIFATVVVGIAKMGDMKEVGRVGVKAL
FYFEAVTTLALVIGLIVVNLLQPGAGLNIDPHTLHAKDIAKFTAGAKEMNTVDFLLHIIPDTVVGAFASGEILQVLTFSV
LLGLALTKMGDNGKTIVHILDEFSHALFGVINMLMKLAPIGAFGAMAFTIGKYGIGSLKQLGFLMACVYLTCFAFVFIVL
GAIARFSGFSLWRFLVYIKEEILLVLGTSSSESALPGIMKKLENLGCSKPVVGMVVPTGYSFNLDGTSIYLTMAAIFIAQ
ATNVNLTLGEELAIIGVLLLTSKGAAAVTGGGFITLAATLATLGGKLPVEGLALLIGIDRFMSEARAITNLIGNGVATVV
IAKWEKALDVERMRQVLEGSEPPAPPKTKRETMPEPALQTR

Sequences:

>Translated_441_residues
MQTKTPFFSRLYVQVLIAIALGVTLGALAPDLGAKMKPLGDAFIKLIKMMIAPIIFATVVVGIAKMGDMKEVGRVGVKAL
FYFEAVTTLALVIGLIVVNLLQPGAGLNIDPHTLHAKDIAKFTAGAKEMNTVDFLLHIIPDTVVGAFASGEILQVLTFSV
LLGLALTKMGDNGKTIVHILDEFSHALFGVINMLMKLAPIGAFGAMAFTIGKYGIGSLKQLGFLMACVYLTCFAFVFIVL
GAIARFSGFSLWRFLVYIKEEILLVLGTSSSESALPGIMKKLENLGCSKPVVGMVVPTGYSFNLDGTSIYLTMAAIFIAQ
ATNVNLTLGEELAIIGVLLLTSKGAAAVTGGGFITLAATLATLGGKLPVEGLALLIGIDRFMSEARAITNLIGNGVATVV
IAKWEKALDVERMRQVLEGSEPPAPPKTKRETMPEPALQTR
>Mature_441_residues
MQTKTPFFSRLYVQVLIAIALGVTLGALAPDLGAKMKPLGDAFIKLIKMMIAPIIFATVVVGIAKMGDMKEVGRVGVKAL
FYFEAVTTLALVIGLIVVNLLQPGAGLNIDPHTLHAKDIAKFTAGAKEMNTVDFLLHIIPDTVVGAFASGEILQVLTFSV
LLGLALTKMGDNGKTIVHILDEFSHALFGVINMLMKLAPIGAFGAMAFTIGKYGIGSLKQLGFLMACVYLTCFAFVFIVL
GAIARFSGFSLWRFLVYIKEEILLVLGTSSSESALPGIMKKLENLGCSKPVVGMVVPTGYSFNLDGTSIYLTMAAIFIAQ
ATNVNLTLGEELAIIGVLLLTSKGAAAVTGGGFITLAATLATLGGKLPVEGLALLIGIDRFMSEARAITNLIGNGVATVV
IAKWEKALDVERMRQVLEGSEPPAPPKTKRETMPEPALQTR

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family

Homologues:

Organism=Homo sapiens, GI40254478, Length=443, Percent_Identity=26.6365688487585, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI169790839, Length=448, Percent_Identity=26.7857142857143, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI66773030, Length=463, Percent_Identity=25.2699784017279, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI21314632, Length=443, Percent_Identity=25.7336343115124, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI194239697, Length=461, Percent_Identity=24.295010845987, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI4827012, Length=383, Percent_Identity=26.3707571801567, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI5032093, Length=379, Percent_Identity=27.7044854881267, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI223468564, Length=255, Percent_Identity=28.6274509803922, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI223468566, Length=255, Percent_Identity=28.6274509803922, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI262359914, Length=373, Percent_Identity=26.0053619302949, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI301601644, Length=191, Percent_Identity=28.7958115183246, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1789947, Length=419, Percent_Identity=61.8138424821002, Blast_Score=532, Evalue=1e-152,
Organism=Escherichia coli, GI1790514, Length=422, Percent_Identity=39.0995260663507, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI1788024, Length=389, Percent_Identity=21.0796915167095, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17537407, Length=436, Percent_Identity=26.605504587156, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI71983106, Length=453, Percent_Identity=26.4900662251656, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71983099, Length=453, Percent_Identity=26.4900662251656, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI193206505, Length=456, Percent_Identity=24.5614035087719, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71996953, Length=415, Percent_Identity=25.3012048192771, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI193206654, Length=451, Percent_Identity=24.6119733924612, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17541374, Length=450, Percent_Identity=24.2222222222222, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24583025, Length=398, Percent_Identity=23.8693467336683, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17137668, Length=398, Percent_Identity=23.8693467336683, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24583023, Length=398, Percent_Identity=23.8693467336683, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI281360483, Length=457, Percent_Identity=23.1947483588621, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI17137666, Length=457, Percent_Identity=23.1947483588621, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI281360481, Length=457, Percent_Identity=23.1947483588621, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DCTA_CHRVO (Q7NRS3)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903377.1
- ProteinModelPortal:   Q7NRS3
- SMR:   Q7NRS3
- GeneID:   2548028
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3707
- NMPDR:   fig|243365.1.peg.3707
- HOGENOM:   HBG739804
- OMA:   HVIFRIV
- PhylomeDB:   Q7NRS3
- BioCyc:   CVIO243365:CV_3707-MONOMER
- HAMAP:   MF_01300
- InterPro:   IPR001991
- InterPro:   IPR018107
- PANTHER:   PTHR11958
- PRINTS:   PR00173

Pfam domain/function: PF00375 SDF

EC number: NA

Molecular weight: Translated: 46735; Mature: 46735

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xf7bc5f0)-; HASH(0x13410260)-; HASH(0x12b71984)-; HASH(0x133071b4)-; HASH(0x135eeb2c)-; HASH(0x13756df8)-; HASH(0x12c50d6c)-; HASH(0x1374837c)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCHHCCCCCCCCCH
>Mature Secondary Structure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CCCCCCCCCHHCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 14500782