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The map label for this gene is rpoS [H]

Identifier: 34499137

GI number: 34499137

Start: 3983111

End: 3984061

Strand: Direct

Name: rpoS [H]

Synonym: CV_3682

Alternate gene names: 34499137

Gene position: 3983111-3984061 (Clockwise)

Preceding gene: 34499136

Following gene: 34499141

Centisome position: 83.84

GC content: 61.72

Gene sequence:

>951_bases
ATGAGTAACGAAATAGACATCCTGGACGAGGATGACATCCTCGACGAAGAAGGCGGCGAGGAAAACGCCGCCGAAGCCGA
GGCAGAAGAAAGCAGCGAAAGCACCGCTGTATTCGAAGACGTGGTCGCCGACGTCACCCAGATTTATCTGAACGACATCG
GCAACAACGCGCTGCTGACGCCGCAGGAGGAACTTGCGCTGGCCCGCCGCGTGGTGGCTGGCGAGTTCGAAGCCCGCCAG
AAGATGATCGAGCACAATCTTCGGCTGGTGGTGAACATCGCCAAGCACTACATCAACCGCGGCCTCGCCCTGCTCGACCT
GATCGAGGAAGGCAACATCGGCCTGATGCACGCGCTGGAGAAATTCGACCCGGAGCGCGGCTTCCGCTTCTCCACCTATG
CCACCTGGTGGATACGCCAGAGCATAGAGCGGGCGATCATGAACCAGTCCCGCACCATCCGCTTGCCGGTGCACGTGATC
AAGGAACTGAACGTCTATCTGCGCGCCTCGCGCCACCTGGAAAGCCAGATTGGCCGGGAACCCAGCGTGGAAGAGATCGC
CCACCTGGTCGGCAAGCCGGTGGAGGACGTGCGCAAGGTGCTGAACCTGAACGAGCGCATGGCCTCGCTGGACGCGCCGC
TGGACATCGACCCCATGCTGTCCATCGGGGAGTCGATTCCGGACGAGCAGCATGAGGAGCCGGAGCTGCAGCTCCACAAC
AGCCAGCTGGAAATGTTCGTCCACGACTGGCTGAGCCAGCTGAACGAAAAGCAAAGGCTGGTGATAGAGCGGCGCTACGG
TCTCAACGGCTACGAGATCTGCACGCTGGAAGAGCTGGCCGCGTCGTTGAACCTGACCCGCGAGCGCGTCCGGCAGATCC
AGATCGAAGGCCTGGAGCAGTTGCGCCGCATTCTGCGCCGCAAGGGCATCTCCCGCGACTTCCTGCTCTGA

Upstream 100 bases:

>100_bases
ACGGCAAGCCGGTGGATCCGATGCAGTATCTTGAACACAAGTCCTGACCGGGCGTAGCCAGCTACTCCCGCCGGGACAAG
ACAGCGACGGGGGTAGAGAG

Downstream 100 bases:

>100_bases
TTCCGCATCGCCCAATCGGCCCGGCCCCGTAGCCGGGCCTTGTCATTTCCAGCGCCGCGCCCGTTTCCGCCAGCGCCTTT
CGCGCGCAGCGTCCGCTTCG

Product: RNA polymerase sigma factor RpoS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSNEIDILDEDDILDEEGGEENAAEAEAEESSESTAVFEDVVADVTQIYLNDIGNNALLTPQEELALARRVVAGEFEARQ
KMIEHNLRLVVNIAKHYINRGLALLDLIEEGNIGLMHALEKFDPERGFRFSTYATWWIRQSIERAIMNQSRTIRLPVHVI
KELNVYLRASRHLESQIGREPSVEEIAHLVGKPVEDVRKVLNLNERMASLDAPLDIDPMLSIGESIPDEQHEEPELQLHN
SQLEMFVHDWLSQLNEKQRLVIERRYGLNGYEICTLEELAASLNLTRERVRQIQIEGLEQLRRILRRKGISRDFLL

Sequences:

>Translated_316_residues
MSNEIDILDEDDILDEEGGEENAAEAEAEESSESTAVFEDVVADVTQIYLNDIGNNALLTPQEELALARRVVAGEFEARQ
KMIEHNLRLVVNIAKHYINRGLALLDLIEEGNIGLMHALEKFDPERGFRFSTYATWWIRQSIERAIMNQSRTIRLPVHVI
KELNVYLRASRHLESQIGREPSVEEIAHLVGKPVEDVRKVLNLNERMASLDAPLDIDPMLSIGESIPDEQHEEPELQLHN
SQLEMFVHDWLSQLNEKQRLVIERRYGLNGYEICTLEELAASLNLTRERVRQIQIEGLEQLRRILRRKGISRDFLL
>Mature_315_residues
SNEIDILDEDDILDEEGGEENAAEAEAEESSESTAVFEDVVADVTQIYLNDIGNNALLTPQEELALARRVVAGEFEARQK
MIEHNLRLVVNIAKHYINRGLALLDLIEEGNIGLMHALEKFDPERGFRFSTYATWWIRQSIERAIMNQSRTIRLPVHVIK
ELNVYLRASRHLESQIGREPSVEEIAHLVGKPVEDVRKVLNLNERMASLDAPLDIDPMLSIGESIPDEQHEEPELQLHNS
QLEMFVHDWLSQLNEKQRLVIERRYGLNGYEICTLEELAASLNLTRERVRQIQIEGLEQLRRILRRKGISRDFLL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is required for the normal expression of the enterotoxin, yst, in the stationary phase [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=309, Percent_Identity=52.4271844660194, Blast_Score=325, Evalue=3e-90,
Organism=Escherichia coli, GI1789448, Length=269, Percent_Identity=42.3791821561338, Blast_Score=207, Evalue=7e-55,
Organism=Escherichia coli, GI1789871, Length=265, Percent_Identity=30.188679245283, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788231, Length=204, Percent_Identity=29.9019607843137, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 36375; Mature: 36244

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNEIDILDEDDILDEEGGEENAAEAEAEESSESTAVFEDVVADVTQIYLNDIGNNALLT
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
PQEELALARRVVAGEFEARQKMIEHNLRLVVNIAKHYINRGLALLDLIEEGNIGLMHALE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHH
KFDPERGFRFSTYATWWIRQSIERAIMNQSRTIRLPVHVIKELNVYLRASRHLESQIGRE
HCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCC
PSVEEIAHLVGKPVEDVRKVLNLNERMASLDAPLDIDPMLSIGESIPDEQHEEPELQLHN
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHH
SQLEMFVHDWLSQLNEKQRLVIERRYGLNGYEICTLEELAASLNLTRERVRQIQIEGLEQ
HHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
LRRILRRKGISRDFLL
HHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SNEIDILDEDDILDEEGGEENAAEAEAEESSESTAVFEDVVADVTQIYLNDIGNNALLT
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
PQEELALARRVVAGEFEARQKMIEHNLRLVVNIAKHYINRGLALLDLIEEGNIGLMHALE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHH
KFDPERGFRFSTYATWWIRQSIERAIMNQSRTIRLPVHVIKELNVYLRASRHLESQIGRE
HCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCC
PSVEEIAHLVGKPVEDVRKVLNLNERMASLDAPLDIDPMLSIGESIPDEQHEEPELQLHN
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHH
SQLEMFVHDWLSQLNEKQRLVIERRYGLNGYEICTLEELAASLNLTRERVRQIQIEGLEQ
HHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
LRRILRRKGISRDFLL
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7729893 [H]