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The map label for this gene is ntrB [H]

Identifier: 34499046

GI number: 34499046

Start: 3899082

End: 3900140

Strand: Direct

Name: ntrB [H]

Synonym: CV_3591

Alternate gene names: 34499046

Gene position: 3899082-3900140 (Clockwise)

Preceding gene: 34499045

Following gene: 34499047

Centisome position: 82.07

GC content: 67.33

Gene sequence:

>1059_bases
ATGACGATACCCAGCTTTGCCGGACTGGAGCTCCTGGACACCCCGGTGCTGATCTGCGATGCCGGAGCCGCGCTGCGCTT
CGTCAATCCCGCCTGCGAGAACCTGCTGGCCCTGGGCAGCCGGGAACTGCTGCGCCATGCGCTGCCCGAGCTGTTCCAGC
CCTGCCCGGCGCTGAATCAGGCGCTCGCCACCGCGCTCGCCCAGGGCGCCAGCTACATCGAGCACGATCTGGAGCTGAAG
CCGCTGCACAACGACTCGGCGCTGCACGTGATGCTGTCGATCACGCCGATAGACGTCGAAGGCTGCCAGGCACTGATCGA
ACTCAGGCCGCTGGACCAGCAGCTGAAGATCGCCAACGAGGAGCGCTCGCTGCTGCAGCACCAGGCCAACCGCGAGCTGA
TCCGCAACCTGGCCCACGAGATCAAAAACCCGCTGGGCGGCATACGCGGCGCGGCGCAGCTGTTGGAGCACGAGCTGGCA
GACCGGCCCGAGCTCAAGGAGTACACCGAGGTGATCACCGAGGAGGCGCTGCGGCTGCAAACGCTGGTAGACCGGCTGCT
GGCGCCGCACCGCAGCCACAACCGCGGGCCGATCAACATCCACGAAGTGCTGGAGCGGGTGCGCAGCATCGCGCTGGCCC
AGCATCCGCAGGGCCTGACCGTCGTCCGCGACTACGACACCAGCCTGCCGCTGCTCTCCGCCGACAAGGAACAGCTGATC
CAGGTGGTGCTGAACATCGTCAACAACGCGGTGCAAGCCTTGCGCGGCCAGGGGCGCATCACGCTGCGCACCCGGGTGGC
GCGGCAGATCACGCTGGCGCGCAAGCGCCATCAGCTGGCACTGAAATTGCAGATCATCGACGACGGTCCCGGCATACCCG
ACGACATCCGCGACCACATCTTCTATCCGCTGGTCACCGGCCGCGCCGAAGGCACCGGCCTGGGGCTGACGCTGGCCCAG
GCCTTCGTCCACCAGCACGGCGGCAGCATAGAGTTCGATTCGCGGCCGGGCCAGACCTGCTTCACCGTGCTGCTGCCCTT
CGGCGGCGAAGCGGGATAA

Upstream 100 bases:

>100_bases
ACTTGAAGCTGAACGCGCGCGCCTCCCCGCGGAATCCAGCCGGCGGGCTGGCGCGCTTATTGCTGCTCTCCGCGCGACAC
CCATAGATCAAGCCTACCCC

Downstream 100 bases:

>100_bases
CGACAAGAGAACACGAGGAGAAACGCCATGGCCAAGCCGGTCTGGATCATAGACGACGACAAGGCGATACGCTGGGTGCT
GGAAAAGGCGCTGACCCGCG

Product: two-component sensor NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MTIPSFAGLELLDTPVLICDAGAALRFVNPACENLLALGSRELLRHALPELFQPCPALNQALATALAQGASYIEHDLELK
PLHNDSALHVMLSITPIDVEGCQALIELRPLDQQLKIANEERSLLQHQANRELIRNLAHEIKNPLGGIRGAAQLLEHELA
DRPELKEYTEVITEEALRLQTLVDRLLAPHRSHNRGPINIHEVLERVRSIALAQHPQGLTVVRDYDTSLPLLSADKEQLI
QVVLNIVNNAVQALRGQGRITLRTRVARQITLARKRHQLALKLQIIDDGPGIPDDIRDHIFYPLVTGRAEGTGLGLTLAQ
AFVHQHGGSIEFDSRPGQTCFTVLLPFGGEAG

Sequences:

>Translated_352_residues
MTIPSFAGLELLDTPVLICDAGAALRFVNPACENLLALGSRELLRHALPELFQPCPALNQALATALAQGASYIEHDLELK
PLHNDSALHVMLSITPIDVEGCQALIELRPLDQQLKIANEERSLLQHQANRELIRNLAHEIKNPLGGIRGAAQLLEHELA
DRPELKEYTEVITEEALRLQTLVDRLLAPHRSHNRGPINIHEVLERVRSIALAQHPQGLTVVRDYDTSLPLLSADKEQLI
QVVLNIVNNAVQALRGQGRITLRTRVARQITLARKRHQLALKLQIIDDGPGIPDDIRDHIFYPLVTGRAEGTGLGLTLAQ
AFVHQHGGSIEFDSRPGQTCFTVLLPFGGEAG
>Mature_351_residues
TIPSFAGLELLDTPVLICDAGAALRFVNPACENLLALGSRELLRHALPELFQPCPALNQALATALAQGASYIEHDLELKP
LHNDSALHVMLSITPIDVEGCQALIELRPLDQQLKIANEERSLLQHQANRELIRNLAHEIKNPLGGIRGAAQLLEHELAD
RPELKEYTEVITEEALRLQTLVDRLLAPHRSHNRGPINIHEVLERVRSIALAQHPQGLTVVRDYDTSLPLLSADKEQLIQ
VVLNIVNNAVQALRGQGRITLRTRVARQITLARKRHQLALKLQIIDDGPGIPDDIRDHIFYPLVTGRAEGTGLGLTLAQA
FVHQHGGSIEFDSRPGQTCFTVLLPFGGEAG

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=333, Percent_Identity=44.7447447447448, Blast_Score=261, Evalue=6e-71,
Organism=Escherichia coli, GI1788549, Length=344, Percent_Identity=29.0697674418605, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1790436, Length=241, Percent_Identity=32.7800829875519, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI87082128, Length=253, Percent_Identity=26.4822134387352, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1786912, Length=248, Percent_Identity=31.4516129032258, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789149, Length=258, Percent_Identity=26.7441860465116, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI48994928, Length=232, Percent_Identity=27.5862068965517, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 38755; Mature: 38624

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIPSFAGLELLDTPVLICDAGAALRFVNPACENLLALGSRELLRHALPELFQPCPALNQ
CCCCCCCCCHHHCCCEEEECCCCHHHHCCHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHH
ALATALAQGASYIEHDLELKPLHNDSALHVMLSITPIDVEGCQALIELRPLDQQLKIANE
HHHHHHHHHHHHHHHCCEECCCCCCCEEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHH
ERSLLQHQANRELIRNLAHEIKNPLGGIRGAAQLLEHELADRPELKEYTEVITEEALRLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
TLVDRLLAPHRSHNRGPINIHEVLERVRSIALAQHPQGLTVVRDYDTSLPLLSADKEQLI
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHH
QVVLNIVNNAVQALRGQGRITLRTRVARQITLARKRHQLALKLQIIDDGPGIPDDIRDHI
HHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCHHHHHHE
FYPLVTGRAEGTGLGLTLAQAFVHQHGGSIEFDSRPGQTCFTVLLPFGGEAG
EHHHEECCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCEEEEEEEECCCCCC
>Mature Secondary Structure 
TIPSFAGLELLDTPVLICDAGAALRFVNPACENLLALGSRELLRHALPELFQPCPALNQ
CCCCCCCCHHHCCCEEEECCCCHHHHCCHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHH
ALATALAQGASYIEHDLELKPLHNDSALHVMLSITPIDVEGCQALIELRPLDQQLKIANE
HHHHHHHHHHHHHHHCCEECCCCCCCEEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHH
ERSLLQHQANRELIRNLAHEIKNPLGGIRGAAQLLEHELADRPELKEYTEVITEEALRLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
TLVDRLLAPHRSHNRGPINIHEVLERVRSIALAQHPQGLTVVRDYDTSLPLLSADKEQLI
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHH
QVVLNIVNNAVQALRGQGRITLRTRVARQITLARKRHQLALKLQIIDDGPGIPDDIRDHI
HHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCHHHHHHE
FYPLVTGRAEGTGLGLTLAQAFVHQHGGSIEFDSRPGQTCFTVLLPFGGEAG
EHHHEECCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]