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The map label for this gene is glnG [H]

Identifier: 34499047

GI number: 34499047

Start: 3900168

End: 3901580

Strand: Direct

Name: glnG [H]

Synonym: CV_3592

Alternate gene names: 34499047

Gene position: 3900168-3901580 (Clockwise)

Preceding gene: 34499046

Following gene: 34499048

Centisome position: 82.09

GC content: 68.44

Gene sequence:

>1413_bases
ATGGCCAAGCCGGTCTGGATCATAGACGACGACAAGGCGATACGCTGGGTGCTGGAAAAGGCGCTGACCCGCGCCGGCAT
CGGCTTCGACAGCTTCGCCAGCGCCGACGACGCGCTCAACGCGCTATACGACGACAAGCCGCAAGCCATCATCTCCGACA
TCCGCATGCCGGGCACCGACGGCCTGAAGTTCCTGTCCAAGGTGAAGACCGAGCATCCGCAGCTGCCGGTCATCATCATG
ACCGCCCACTCCGACCTGGACTCCGCGGTGTCGGCCTTCCAGGGCGGCGCCTTCGAATACCTGCCCAAGCCGTTCGACAT
CGACCAGGCGGTGCTGCTGATCGAGCGCGCGCTGGCCGAAAGCGAGGCGCAAGGCGGCGGCGCCGACAGCCCGGAAGCGA
TGCCGGTGCTGCTGGGCCAGGCGCCGGCGATGCAGGACGTATTCCGCGCCATCGGCCGGCTGGCCCAATCCAACGTCACC
GTGCTGATCACCGGAGAATCCGGCACCGGCAAGGAACGGGTGGCCGAGGCGCTGCACCGCCACTCGGTGCGCGCCGGCAA
GCCCTTCATCGCGCTCAACACCGCGGCGATACCGAAGGACCTGCTGGAATCCGAACTGTTCGGCCACGAAAAAGGCGCGT
TTACCGGCGCGCTGGCCACGCGGCGCGGCCGCTTCGAGGAAGCCGAAGGCGGCACGCTGTTCCTGGACGAGATCGGCGAC
ATGCCGACCGAGCTGCAAACCCGGCTGCTCAGAGTGCTGTCCGACGGCCATTTCTACCGCGTCGGCGGCCACACGCCGAT
CAAGGCCAATGTCCGGGTGATCGCCGCCACCCACCAGAACCTGGAGCAACGGGTGCGCGACGGGCTGTTCCGCGAAGACC
TGTACCACCGCCTCAACGTGATCCGCCTGCGGCTGCCGCCGCTGAGGGAGCGGCGCGAGGACATCCCGCTCTTGGCCCGC
CACTTCCTGGCCAAGAGCGCCTCCCAGCTGGGCGTCGAACCCAAGCGCCTGTCCGAGGCGGCGATGGCGCTGATCCAGCG
CAGCGCTTTCACCGGCAACGTGCGCGAGCTGGAAAACCTGTGCCAGTGGATATGCGTGATGGCGCCGGGCCAGACCGTCG
AAGTGGCCGACCTGCCGCCGGAGCTGCGCGAGCCGCAAGCCGAGGCCGCCGGTTGCCCGCACGGCGACTGGGACCGGATG
CTGGAGGACGAGATCCGCCGGCGTCTGCGCGCCGGCGAGGTCGGCATCGTCGATGAGCTGACGCGCCGCTTCGAAACCAC
CTGCATCCGCGCCAGCCTCGGCCATACCGGCGGGCGCCGGGTGGAAGCCGCGCACCTGCTGGGCTGGGGGCGCAACACCC
TGACCCGCAAGATTCAGGAACTAGGGCTGGAGCACGACGCTCAGCACAGTTAA

Upstream 100 bases:

>100_bases
CATAGAGTTCGATTCGCGGCCGGGCCAGACCTGCTTCACCGTGCTGCTGCCCTTCGGCGGCGAAGCGGGATAACGACAAG
AGAACACGAGGAGAAACGCC

Downstream 100 bases:

>100_bases
TTGTTAATCAAATAATTCCAAAACAATCAGCATATTTTTAATCATTTTGATAAAATGGGCAGACCTTTCCCAGCCTGAAT
GTCCGATGTCCCGTCTGCCC

Product: nitrogen assimilation regulatory response regulator/transcription regulator protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MAKPVWIIDDDKAIRWVLEKALTRAGIGFDSFASADDALNALYDDKPQAIISDIRMPGTDGLKFLSKVKTEHPQLPVIIM
TAHSDLDSAVSAFQGGAFEYLPKPFDIDQAVLLIERALAESEAQGGGADSPEAMPVLLGQAPAMQDVFRAIGRLAQSNVT
VLITGESGTGKERVAEALHRHSVRAGKPFIALNTAAIPKDLLESELFGHEKGAFTGALATRRGRFEEAEGGTLFLDEIGD
MPTELQTRLLRVLSDGHFYRVGGHTPIKANVRVIAATHQNLEQRVRDGLFREDLYHRLNVIRLRLPPLRERREDIPLLAR
HFLAKSASQLGVEPKRLSEAAMALIQRSAFTGNVRELENLCQWICVMAPGQTVEVADLPPELREPQAEAAGCPHGDWDRM
LEDEIRRRLRAGEVGIVDELTRRFETTCIRASLGHTGGRRVEAAHLLGWGRNTLTRKIQELGLEHDAQHS

Sequences:

>Translated_470_residues
MAKPVWIIDDDKAIRWVLEKALTRAGIGFDSFASADDALNALYDDKPQAIISDIRMPGTDGLKFLSKVKTEHPQLPVIIM
TAHSDLDSAVSAFQGGAFEYLPKPFDIDQAVLLIERALAESEAQGGGADSPEAMPVLLGQAPAMQDVFRAIGRLAQSNVT
VLITGESGTGKERVAEALHRHSVRAGKPFIALNTAAIPKDLLESELFGHEKGAFTGALATRRGRFEEAEGGTLFLDEIGD
MPTELQTRLLRVLSDGHFYRVGGHTPIKANVRVIAATHQNLEQRVRDGLFREDLYHRLNVIRLRLPPLRERREDIPLLAR
HFLAKSASQLGVEPKRLSEAAMALIQRSAFTGNVRELENLCQWICVMAPGQTVEVADLPPELREPQAEAAGCPHGDWDRM
LEDEIRRRLRAGEVGIVDELTRRFETTCIRASLGHTGGRRVEAAHLLGWGRNTLTRKIQELGLEHDAQHS
>Mature_469_residues
AKPVWIIDDDKAIRWVLEKALTRAGIGFDSFASADDALNALYDDKPQAIISDIRMPGTDGLKFLSKVKTEHPQLPVIIMT
AHSDLDSAVSAFQGGAFEYLPKPFDIDQAVLLIERALAESEAQGGGADSPEAMPVLLGQAPAMQDVFRAIGRLAQSNVTV
LITGESGTGKERVAEALHRHSVRAGKPFIALNTAAIPKDLLESELFGHEKGAFTGALATRRGRFEEAEGGTLFLDEIGDM
PTELQTRLLRVLSDGHFYRVGGHTPIKANVRVIAATHQNLEQRVRDGLFREDLYHRLNVIRLRLPPLRERREDIPLLARH
FLAKSASQLGVEPKRLSEAAMALIQRSAFTGNVRELENLCQWICVMAPGQTVEVADLPPELREPQAEAAGCPHGDWDRML
EDEIRRRLRAGEVGIVDELTRRFETTCIRASLGHTGGRRVEAAHLLGWGRNTLTRKIQELGLEHDAQHS

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=464, Percent_Identity=61.2068965517241, Blast_Score=563, Evalue=1e-161,
Organism=Escherichia coli, GI1788550, Length=469, Percent_Identity=41.7910447761194, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1790437, Length=457, Percent_Identity=40.4814004376368, Blast_Score=308, Evalue=7e-85,
Organism=Escherichia coli, GI1788905, Length=362, Percent_Identity=43.646408839779, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI87082117, Length=333, Percent_Identity=44.1441441441441, Blast_Score=244, Evalue=9e-66,
Organism=Escherichia coli, GI1789087, Length=359, Percent_Identity=41.5041782729805, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI1789233, Length=351, Percent_Identity=39.8860398860399, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI87082152, Length=335, Percent_Identity=40.2985074626866, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1786524, Length=262, Percent_Identity=47.3282442748092, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI1787583, Length=344, Percent_Identity=37.5, Blast_Score=206, Evalue=4e-54,
Organism=Escherichia coli, GI87081872, Length=227, Percent_Identity=45.374449339207, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI87081858, Length=310, Percent_Identity=31.9354838709677, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1789828, Length=227, Percent_Identity=35.6828193832599, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1789809, Length=136, Percent_Identity=27.2058823529412, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51866; Mature: 51735

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKPVWIIDDDKAIRWVLEKALTRAGIGFDSFASADDALNALYDDKPQAIISDIRMPGTD
CCCCEEEEECCHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHCCCHHHHHHHCCCCCCH
GLKFLSKVKTEHPQLPVIIMTAHSDLDSAVSAFQGGAFEYLPKPFDIDQAVLLIERALAE
HHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHH
SEAQGGGADSPEAMPVLLGQAPAMQDVFRAIGRLAQSNVTVLITGESGTGKERVAEALHR
HHCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHH
HSVRAGKPFIALNTAAIPKDLLESELFGHEKGAFTGALATRRGRFEEAEGGTLFLDEIGD
HHHCCCCCEEEEECHHCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCEEEHHHHCC
MPTELQTRLLRVLSDGHFYRVGGHTPIKANVRVIAATHQNLEQRVRDGLFREDLYHRLNV
CCHHHHHHHHHHHCCCCEEEECCCCCEECCEEEEEEHHHHHHHHHHHCHHHHHHHHHHHE
IRLRLPPLRERREDIPLLARHFLAKSASQLGVEPKRLSEAAMALIQRSAFTGNVRELENL
EEEECCCHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
CQWICVMAPGQTVEVADLPPELREPQAEAAGCPHGDWDRMLEDEIRRRLRAGEVGIVDEL
HHHHHHCCCCCEEEECCCCHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
TRRFETTCIRASLGHTGGRRVEAAHLLGWGRNTLTRKIQELGLEHDAQHS
HHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AKPVWIIDDDKAIRWVLEKALTRAGIGFDSFASADDALNALYDDKPQAIISDIRMPGTD
CCCEEEEECCHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHCCCHHHHHHHCCCCCCH
GLKFLSKVKTEHPQLPVIIMTAHSDLDSAVSAFQGGAFEYLPKPFDIDQAVLLIERALAE
HHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHH
SEAQGGGADSPEAMPVLLGQAPAMQDVFRAIGRLAQSNVTVLITGESGTGKERVAEALHR
HHCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHH
HSVRAGKPFIALNTAAIPKDLLESELFGHEKGAFTGALATRRGRFEEAEGGTLFLDEIGD
HHHCCCCCEEEEECHHCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCEEEHHHHCC
MPTELQTRLLRVLSDGHFYRVGGHTPIKANVRVIAATHQNLEQRVRDGLFREDLYHRLNV
CCHHHHHHHHHHHCCCCEEEECCCCCEECCEEEEEEHHHHHHHHHHHCHHHHHHHHHHHE
IRLRLPPLRERREDIPLLARHFLAKSASQLGVEPKRLSEAAMALIQRSAFTGNVRELENL
EEEECCCHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
CQWICVMAPGQTVEVADLPPELREPQAEAAGCPHGDWDRMLEDEIRRRLRAGEVGIVDEL
HHHHHHCCCCCEEEECCCCHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
TRRFETTCIRASLGHTGGRRVEAAHLLGWGRNTLTRKIQELGLEHDAQHS
HHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]