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The map label for this gene is ispH

Identifier: 34499022

GI number: 34499022

Start: 3873454

End: 3874389

Strand: Reverse

Name: ispH

Synonym: CV_3567

Alternate gene names: 34499022

Gene position: 3874389-3873454 (Counterclockwise)

Preceding gene: 34499023

Following gene: 34499020

Centisome position: 81.55

GC content: 66.13

Gene sequence:

>936_bases
ATGACGAAGACCATCATGCTCGCCAACCCGCGCGGCTTCTGCGCCGGCGTGGACCGGGCCATCGCCATCGTCGAGCGCGC
GATCGAACTTTACGGCGCCCCCATCTACGTGCGCCACGAAGTGGTGCACAACCGTTTTGTGGTGGACGACCTGCGCGGCA
AGGGCGCGGTGTTCATCGAGGAATTGAAGGACGTGCCCCCCGGCAGCACGCTGATCTATTCCGCGCACGGCGTGCCGCTG
TCGGTGCGCGCCGAGGCCGAGGCGCTGGGTCTGACCGTATTCGACGCCACCTGTCCCTTGGTGACCAAGGTGCACGTCGA
AGTGAAACGGATGAACAAGGCCGGCATGGAAATCATCATGATCGGCCACGCCGGGCACCCGGAAGTGGAAGGCACGATGG
GCCAGGTGGATGCCGGCATCTACCTGGTGCAGACCGTGGACGATGTGGCCACGCTGCGGGTGGTCAACGAGGACCAGCTG
TCCTACGTCAGCCAGACCACGCTGTCGGTGGACGAGACCCGCGACATCATCGCCGCGCTGAAGGCCCGCTTCCCCAACAT
CCACAGCCCGAAGAAGGACGACATCTGCTACGCGACGCAGAACCGCCAGGACGCGGTGAAGCTGCTGGCCGACCAGTGCG
ATATCGTGGTGGTGGTGGGCTCGCCCAACAGCTCCAACTCCAACCGCTTGCGCGAAGTGGCGGCGCTGAAGGGCGTGGAC
GCCTACATGGTGGACAACGCCAGCCTGCTGGAGCCCGAATGGTTCGCCGGCAAGCGCCGCGTCGGCGTCACCGCCGGCGC
CAGCGCGCCGGAAGTGCTGGTGCAGGCGGTGATAGACCGCATCAAGGCTTTCGACGTCACCCAGGTCACCGAGCTGCCCG
GCGTAGAGGAGAGCACGGTGTTCGCGCTGCCTCCGGCGCTGCGCCCGCTGTTCTGA

Upstream 100 bases:

>100_bases
TCGCGCTGAAAAGGGCGGGGAACAGCACCTGCGATGGCCCGCTTGGCGGGCCATTCGCGCATTCGCCGGGCAAGGCCCTG
GCCTGATTGAGGAAATACGG

Downstream 100 bases:

>100_bases
CGCGGGTTTTATCGCCAAAGCCAAGGCCGCTCCATCTGGAGCGGCCTTTTCCATGGCAGTGGGCCCCTTAGGATTGGGGA
ACAAAACCTGTTGTCGGCGG

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MTKTIMLANPRGFCAGVDRAIAIVERAIELYGAPIYVRHEVVHNRFVVDDLRGKGAVFIEELKDVPPGSTLIYSAHGVPL
SVRAEAEALGLTVFDATCPLVTKVHVEVKRMNKAGMEIIMIGHAGHPEVEGTMGQVDAGIYLVQTVDDVATLRVVNEDQL
SYVSQTTLSVDETRDIIAALKARFPNIHSPKKDDICYATQNRQDAVKLLADQCDIVVVVGSPNSSNSNRLREVAALKGVD
AYMVDNASLLEPEWFAGKRRVGVTAGASAPEVLVQAVIDRIKAFDVTQVTELPGVEESTVFALPPALRPLF

Sequences:

>Translated_311_residues
MTKTIMLANPRGFCAGVDRAIAIVERAIELYGAPIYVRHEVVHNRFVVDDLRGKGAVFIEELKDVPPGSTLIYSAHGVPL
SVRAEAEALGLTVFDATCPLVTKVHVEVKRMNKAGMEIIMIGHAGHPEVEGTMGQVDAGIYLVQTVDDVATLRVVNEDQL
SYVSQTTLSVDETRDIIAALKARFPNIHSPKKDDICYATQNRQDAVKLLADQCDIVVVVGSPNSSNSNRLREVAALKGVD
AYMVDNASLLEPEWFAGKRRVGVTAGASAPEVLVQAVIDRIKAFDVTQVTELPGVEESTVFALPPALRPLF
>Mature_310_residues
TKTIMLANPRGFCAGVDRAIAIVERAIELYGAPIYVRHEVVHNRFVVDDLRGKGAVFIEELKDVPPGSTLIYSAHGVPLS
VRAEAEALGLTVFDATCPLVTKVHVEVKRMNKAGMEIIMIGHAGHPEVEGTMGQVDAGIYLVQTVDDVATLRVVNEDQLS
YVSQTTLSVDETRDIIAALKARFPNIHSPKKDDICYATQNRQDAVKLLADQCDIVVVVGSPNSSNSNRLREVAALKGVDA
YMVDNASLLEPEWFAGKRRVGVTAGASAPEVLVQAVIDRIKAFDVTQVTELPGVEESTVFALPPALRPLF

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=307, Percent_Identity=60.586319218241, Blast_Score=383, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_CHRVO (Q7NS59)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903237.1
- GeneID:   2549893
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3567
- NMPDR:   fig|243365.1.peg.3567
- HOGENOM:   HBG335228
- OMA:   HMQVARA
- PhylomeDB:   Q7NS59
- ProtClustDB:   PRK01045
- BioCyc:   CVIO243365:CV_3567-MONOMER
- BRENDA:   1.17.1.2
- HAMAP:   MF_00191
- InterPro:   IPR013785
- InterPro:   IPR003451
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 33697; Mature: 33566

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTIMLANPRGFCAGVDRAIAIVERAIELYGAPIYVRHEVVHNRFVVDDLRGKGAVFIE
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHCEEEEECCCCCCCEEEH
ELKDVPPGSTLIYSAHGVPLSVRAEAEALGLTVFDATCPLVTKVHVEVKRMNKAGMEIIM
HHHCCCCCCEEEEECCCCEEEEECCHHHEEEEEEECCCCEEEEEEHHEEHHCCCCCEEEE
IGHAGHPEVEGTMGQVDAGIYLVQTVDDVATLRVVNEDQLSYVSQTTLSVDETRDIIAAL
EECCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEECCHHHHHHHHHHCCHHHHHHHHHHH
KARFPNIHSPKKDDICYATQNRQDAVKLLADQCDIVVVVGSPNSSNSNRLREVAALKGVD
HHHCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCC
AYMVDNASLLEPEWFAGKRRVGVTAGASAPEVLVQAVIDRIKAFDVTQVTELPGVEESTV
EEEECCCCCCCCHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEE
FALPPALRPLF
EECCCHHCCCC
>Mature Secondary Structure 
TKTIMLANPRGFCAGVDRAIAIVERAIELYGAPIYVRHEVVHNRFVVDDLRGKGAVFIE
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHCEEEEECCCCCCCEEEH
ELKDVPPGSTLIYSAHGVPLSVRAEAEALGLTVFDATCPLVTKVHVEVKRMNKAGMEIIM
HHHCCCCCCEEEEECCCCEEEEECCHHHEEEEEEECCCCEEEEEEHHEEHHCCCCCEEEE
IGHAGHPEVEGTMGQVDAGIYLVQTVDDVATLRVVNEDQLSYVSQTTLSVDETRDIIAAL
EECCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEECCHHHHHHHHHHCCHHHHHHHHHHH
KARFPNIHSPKKDDICYATQNRQDAVKLLADQCDIVVVVGSPNSSNSNRLREVAALKGVD
HHHCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCC
AYMVDNASLLEPEWFAGKRRVGVTAGASAPEVLVQAVIDRIKAFDVTQVTELPGVEESTV
EEEECCCCCCCCHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEE
FALPPALRPLF
EECCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782