Click here to switch to the map view.

The map label for this gene is hsrA [H]

Identifier: 34498979

GI number: 34498979

Start: 3829976

End: 3831349

Strand: Reverse

Name: hsrA [H]

Synonym: CV_3524

Alternate gene names: 34498979

Gene position: 3831349-3829976 (Counterclockwise)

Preceding gene: 34498980

Following gene: 34498978

Centisome position: 80.64

GC content: 65.57

Gene sequence:

>1374_bases
ATGCTGACGCCGCTCATCGTCGCCACGGCGCTGTTCATGGAAAACATGGACGCCACCGTGATCTCAACCTCGCTGCCGGT
GATCGCCCAGGATCTGGGCGTGGACCCGATCTCGCTGAAGCTGGCGTTGACGTCCTATCTGGTCAGCCTGGCGGTGTTCA
TTCCCATCAGCGGCTGGATGGCTGACCGTTTCGGCGCCCGCCGGATTTTCCGCTCCGCCATCCTGGTGTTCATGCTGGGC
TCGCTGTTGTGCGCGGCCACCGGCAGCCTGCACGGCTTCGTGCTGGCGCGCTTCCTGCAGGGCATAGGCGGGGCGATGAT
GGTGCCGGTGGGGCGCCTGGTGATCCTGCGCACCATAGACAAGGCGGACCTGGTGCGCGCGCTCAGCTACCTGACCGTGC
CGGCGCTGCTGGGACCGGTGATAGGCCCGCCCTTGGGCGGCTTCATCAGCACCTATTTCCATTGGCGCTGGATTTTCCTG
ATCAATATCCCGATCGGCCTCCTGGGGCTGGCGCTGGCCGGACGCTTCATCGCCAACTTGAAGGAGGACGAGGTGCCCAG
GCTGGACTGGTGGGGCTTTCTGCTCACCGGCACCGGGCTGTCGATGCTGATGCTGGGCCTGGCCACCGAGGGCAAGCACA
TGCTGTCGGCCACCGCCTCGATCTGGCTGTCGGCGCTGGGGGCGGCGCTATTGCTGGCCTATCTCCGGCATTACCGCAAG
CAGACGCACCCGTTGCTGGATCTGTCGCTGCTGCGCTTGCCCACCTTCAACGCCGGCGTGGTCGGCGGCTTCATGTTCCG
CATCGGCATCGGCACCATTCCCTTCCTGTTGCCGCTGATGCTGCAGCTGGGTTTCGGTTTCTCGCCGTTCGAGTCGGGCC
TGCTGACTTGCTCCACCGCGATGGGGGCGATGGGCATGAAGACCATCGTCGCCAAGGTGCTGCAACGCTACGGCTTCCGC
CGCGTGCTGGTGGTCAACAGCATCCTGGCAGGCTGCTCGGTGGCGGTGTATTCGCTGTTCCAGGCCGATACGCCGCATTG
GCTGCTGCTGCTGGTGTTCGTGCTGGGCGGGTGCATGCGCTCGCTGCAGTTCACCAGCCTGAACGCGATCACCTTCGCCG
ATGTCGACAAGGACAGGCTGAGCCACGCCACCAGCCTGTCCAGCGTGGCGCAGCAGCTGGCCGCGGGTTTCGGCGTGACC
GTGGGCGCGTTCGCGTTGCAGAGCGTGGCCTGGCTGCAGGGCCATCAGCAGCTGACCGCCGTCGATTTCGGCCATGCCTT
CCTGATCATGGGCGGCCTGACCATGCTGTCCAGCCTGCAGTTCCTGAAGCTGGAGCGGGATGCCGGACGGCAGGTGTCGG
CCGGGCATGAGTGA

Upstream 100 bases:

>100_bases
TAGCGTTCATGACATCCGGCATGGCGGTCGCCGGCAAGTGGTAAGATAAAAAAACTTTCCCCCACACCTTTCTGTCATGC
AGTTGAAAATCGCTCCCGGC

Downstream 100 bases:

>100_bases
TGGCCGCCAGGCGGGGCCGCGTCCCCTGGCGCGCAGCCAGGGGACGTCGGCGCAGGGCTGACGCCTGGCTGGTGAACGCC
CAGGCGTCGCGCTCGCCGAA

Product: multidrug efflux protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MLTPLIVATALFMENMDATVISTSLPVIAQDLGVDPISLKLALTSYLVSLAVFIPISGWMADRFGARRIFRSAILVFMLG
SLLCAATGSLHGFVLARFLQGIGGAMMVPVGRLVILRTIDKADLVRALSYLTVPALLGPVIGPPLGGFISTYFHWRWIFL
INIPIGLLGLALAGRFIANLKEDEVPRLDWWGFLLTGTGLSMLMLGLATEGKHMLSATASIWLSALGAALLLAYLRHYRK
QTHPLLDLSLLRLPTFNAGVVGGFMFRIGIGTIPFLLPLMLQLGFGFSPFESGLLTCSTAMGAMGMKTIVAKVLQRYGFR
RVLVVNSILAGCSVAVYSLFQADTPHWLLLLVFVLGGCMRSLQFTSLNAITFADVDKDRLSHATSLSSVAQQLAAGFGVT
VGAFALQSVAWLQGHQQLTAVDFGHAFLIMGGLTMLSSLQFLKLERDAGRQVSAGHE

Sequences:

>Translated_457_residues
MLTPLIVATALFMENMDATVISTSLPVIAQDLGVDPISLKLALTSYLVSLAVFIPISGWMADRFGARRIFRSAILVFMLG
SLLCAATGSLHGFVLARFLQGIGGAMMVPVGRLVILRTIDKADLVRALSYLTVPALLGPVIGPPLGGFISTYFHWRWIFL
INIPIGLLGLALAGRFIANLKEDEVPRLDWWGFLLTGTGLSMLMLGLATEGKHMLSATASIWLSALGAALLLAYLRHYRK
QTHPLLDLSLLRLPTFNAGVVGGFMFRIGIGTIPFLLPLMLQLGFGFSPFESGLLTCSTAMGAMGMKTIVAKVLQRYGFR
RVLVVNSILAGCSVAVYSLFQADTPHWLLLLVFVLGGCMRSLQFTSLNAITFADVDKDRLSHATSLSSVAQQLAAGFGVT
VGAFALQSVAWLQGHQQLTAVDFGHAFLIMGGLTMLSSLQFLKLERDAGRQVSAGHE
>Mature_457_residues
MLTPLIVATALFMENMDATVISTSLPVIAQDLGVDPISLKLALTSYLVSLAVFIPISGWMADRFGARRIFRSAILVFMLG
SLLCAATGSLHGFVLARFLQGIGGAMMVPVGRLVILRTIDKADLVRALSYLTVPALLGPVIGPPLGGFISTYFHWRWIFL
INIPIGLLGLALAGRFIANLKEDEVPRLDWWGFLLTGTGLSMLMLGLATEGKHMLSATASIWLSALGAALLLAYLRHYRK
QTHPLLDLSLLRLPTFNAGVVGGFMFRIGIGTIPFLLPLMLQLGFGFSPFESGLLTCSTAMGAMGMKTIVAKVLQRYGFR
RVLVVNSILAGCSVAVYSLFQADTPHWLLLLVFVLGGCMRSLQFTSLNAITFADVDKDRLSHATSLSSVAQQLAAGFGVT
VGAFALQSVAWLQGHQQLTAVDFGHAFLIMGGLTMLSSLQFLKLERDAGRQVSAGHE

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=450, Percent_Identity=40.6666666666667, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1788392, Length=455, Percent_Identity=39.1208791208791, Blast_Score=282, Evalue=4e-77,
Organism=Escherichia coli, GI1789042, Length=402, Percent_Identity=25.3731343283582, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1788710, Length=428, Percent_Identity=26.4018691588785, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI87081983, Length=316, Percent_Identity=27.5316455696203, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1790146, Length=135, Percent_Identity=30.3703703703704, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6325455, Length=414, Percent_Identity=23.6714975845411, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6322958, Length=237, Percent_Identity=24.0506329113924, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49230; Mature: 49230

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS00626 RCC1_2 ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTPLIVATALFMENMDATVISTSLPVIAQDLGVDPISLKLALTSYLVSLAVFIPISGWM
CCCHHHHHHHHHHHCCCCHHEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHH
ADRFGARRIFRSAILVFMLGSLLCAATGSLHGFVLARFLQGIGGAMMVPVGRLVILRTID
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
KADLVRALSYLTVPALLGPVIGPPLGGFISTYFHWRWIFLINIPIGLLGLALAGRFIANL
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHCC
KEDEVPRLDWWGFLLTGTGLSMLMLGLATEGKHMLSATASIWLSALGAALLLAYLRHYRK
CCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTHPLLDLSLLRLPTFNAGVVGGFMFRIGIGTIPFLLPLMLQLGFGFSPFESGLLTCSTA
HCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHH
MGAMGMKTIVAKVLQRYGFRRVLVVNSILAGCSVAVYSLFQADTPHWLLLLVFVLGGCMR
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
SLQFTSLNAITFADVDKDRLSHATSLSSVAQQLAAGFGVTVGAFALQSVAWLQGHQQLTA
HHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEH
VDFGHAFLIMGGLTMLSSLQFLKLERDAGRQVSAGHE
HHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLTPLIVATALFMENMDATVISTSLPVIAQDLGVDPISLKLALTSYLVSLAVFIPISGWM
CCCHHHHHHHHHHHCCCCHHEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHH
ADRFGARRIFRSAILVFMLGSLLCAATGSLHGFVLARFLQGIGGAMMVPVGRLVILRTID
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
KADLVRALSYLTVPALLGPVIGPPLGGFISTYFHWRWIFLINIPIGLLGLALAGRFIANL
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHCC
KEDEVPRLDWWGFLLTGTGLSMLMLGLATEGKHMLSATASIWLSALGAALLLAYLRHYRK
CCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTHPLLDLSLLRLPTFNAGVVGGFMFRIGIGTIPFLLPLMLQLGFGFSPFESGLLTCSTA
HCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHH
MGAMGMKTIVAKVLQRYGFRRVLVVNSILAGCSVAVYSLFQADTPHWLLLLVFVLGGCMR
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
SLQFTSLNAITFADVDKDRLSHATSLSSVAQQLAAGFGVTVGAFALQSVAWLQGHQQLTA
HHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEH
VDFGHAFLIMGGLTMLSSLQFLKLERDAGRQVSAGHE
HHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]