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The map label for this gene is nemA1 [H]

Identifier: 34498956

GI number: 34498956

Start: 3794884

End: 3795978

Strand: Direct

Name: nemA1 [H]

Synonym: CV_3501

Alternate gene names: 34498956

Gene position: 3794884-3795978 (Clockwise)

Preceding gene: 34498955

Following gene: 34498957

Centisome position: 79.87

GC content: 68.31

Gene sequence:

>1095_bases
ATGAACGCCGACAAACTGCTGACCCCGCTGACCATGGGCGCCGTCGCCCTGAGCAACCGCGTCGTCATGGCCCCGCTGAC
CCGCCTGCGCAACATCGAACCGGGCGACGTGCCCGGCCCGCTGGCCAAGGAATACTACCGCCAGCGCGCCAGCGCCGGCC
TGATCGTGGCCGAAGGCACCCACATTTCCCCCACCGCCAAGGGCTATGCCGGCGCGCCCGGCATCTACAGCGAGGAACAA
GTGCGCGCCTGGAGCGAAGTCACCGGCGCGGTGCATCAAGACGGCGGCAAGATCGCGCTGCAACTGTGGCACACCGGCCG
CATCTCGCACCGCTCGCTGCAGCCGAACGGCGACGCGCCGGTCGGCCCGTCCGCCATCCAGGCCGACAGCCGCACCAACA
TCCGCGCCGCCGACGGCAGCCTGGTGCGCGAACAATGCGACACCCCGCGCGCGCTGGAAATCGAGGAAATCGAGGACATC
ATCGAGGACTACCGCCGCGCCGCCGACAACGCGCGCCGCGCCGGTTTCGACATGGTGGAGATCCACGGCGCCCACGGCTA
TCTGATCGACCAGTTCCTGTCGCCGGCCGCCAACGTCCGCACCGACCAGTACGGCGGCAGCGTGGAAAACCGCGCCCGCT
TCCTGCTGGAAGTGGTGGACGCGGTGGTGGCGGAATGGGACGCCGATCACGTCGGCATCCGCATCTCGCCGCTGGGCATC
TTCAACGGCGTCAGCAACACCGACCAGCTGGACATGGCGCTGTACCTGGCCGAGCAGTTGGCCAAGCGCAAGCTGGCCTT
CCTGCACATCTCCGAGCCGGACTGGGCCGGCGGCCCGACGCTGGACGACGGCTTCCGCGCCGAACTGCGCCAGCGCTATC
CCGGCGTGATCATCGGCGCCGGCGGCTACTCCGCGGAGAAGGCCGAAACGCTGCTGAAGAAAGGCTTTATCGACGCCGCC
GCCTTCGGCCGCAGCTACATCGCCAACCCGGATCTGGTGGAGCGTCTGAAGCAGAACGCTCCGCTGAACCCGCCCAAGCC
GGATACTTTCTATGGCGGCGGCGCGGAAGGCTATACCGACTACCCGACGCTGTAA

Upstream 100 bases:

>100_bases
TACTACCGCCAGGTTTATTTCCCCAAGGTCGCGCCCGTCGCGCGCGCCCAGGGTTTTGGCTTCGACCAGTAACTCCGACC
ACGCCTTCAAGGAAACCGAC

Downstream 100 bases:

>100_bases
GCCCCGCCCACGGCCCCGCACCGCGCGGGGCTTTTTTCATGCCCTGGCGGTGCCAGACGGACGCCACGCGCGCGTCCGTC
CGGCGCAGCGATGCCGCAGC

Product: N-ethylmaleimide reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MNADKLLTPLTMGAVALSNRVVMAPLTRLRNIEPGDVPGPLAKEYYRQRASAGLIVAEGTHISPTAKGYAGAPGIYSEEQ
VRAWSEVTGAVHQDGGKIALQLWHTGRISHRSLQPNGDAPVGPSAIQADSRTNIRAADGSLVREQCDTPRALEIEEIEDI
IEDYRRAADNARRAGFDMVEIHGAHGYLIDQFLSPAANVRTDQYGGSVENRARFLLEVVDAVVAEWDADHVGIRISPLGI
FNGVSNTDQLDMALYLAEQLAKRKLAFLHISEPDWAGGPTLDDGFRAELRQRYPGVIIGAGGYSAEKAETLLKKGFIDAA
AFGRSYIANPDLVERLKQNAPLNPPKPDTFYGGGAEGYTDYPTL

Sequences:

>Translated_364_residues
MNADKLLTPLTMGAVALSNRVVMAPLTRLRNIEPGDVPGPLAKEYYRQRASAGLIVAEGTHISPTAKGYAGAPGIYSEEQ
VRAWSEVTGAVHQDGGKIALQLWHTGRISHRSLQPNGDAPVGPSAIQADSRTNIRAADGSLVREQCDTPRALEIEEIEDI
IEDYRRAADNARRAGFDMVEIHGAHGYLIDQFLSPAANVRTDQYGGSVENRARFLLEVVDAVVAEWDADHVGIRISPLGI
FNGVSNTDQLDMALYLAEQLAKRKLAFLHISEPDWAGGPTLDDGFRAELRQRYPGVIIGAGGYSAEKAETLLKKGFIDAA
AFGRSYIANPDLVERLKQNAPLNPPKPDTFYGGGAEGYTDYPTL
>Mature_364_residues
MNADKLLTPLTMGAVALSNRVVMAPLTRLRNIEPGDVPGPLAKEYYRQRASAGLIVAEGTHISPTAKGYAGAPGIYSEEQ
VRAWSEVTGAVHQDGGKIALQLWHTGRISHRSLQPNGDAPVGPSAIQADSRTNIRAADGSLVREQCDTPRALEIEEIEDI
IEDYRRAADNARRAGFDMVEIHGAHGYLIDQFLSPAANVRTDQYGGSVENRARFLLEVVDAVVAEWDADHVGIRISPLGI
FNGVSNTDQLDMALYLAEQLAKRKLAFLHISEPDWAGGPTLDDGFRAELRQRYPGVIIGAGGYSAEKAETLLKKGFIDAA
AFGRSYIANPDLVERLKQNAPLNPPKPDTFYGGGAEGYTDYPTL

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=366, Percent_Identity=62.568306010929, Blast_Score=481, Evalue=1e-137,
Organism=Escherichia coli, GI1789463, Length=242, Percent_Identity=38.0165289256198, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17559802, Length=346, Percent_Identity=28.9017341040462, Blast_Score=104, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17565138, Length=352, Percent_Identity=28.4090909090909, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17559804, Length=194, Percent_Identity=35.0515463917526, Blast_Score=101, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI72001454, Length=303, Percent_Identity=29.042904290429, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17564188, Length=180, Percent_Identity=32.7777777777778, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17540738, Length=178, Percent_Identity=33.1460674157303, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17566914, Length=194, Percent_Identity=32.4742268041237, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321973, Length=383, Percent_Identity=38.6422976501305, Blast_Score=233, Evalue=4e-62,
Organism=Saccharomyces cerevisiae, GI6325086, Length=380, Percent_Identity=39.2105263157895, Blast_Score=231, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39446; Mature: 39446

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNADKLLTPLTMGAVALSNRVVMAPLTRLRNIEPGDVPGPLAKEYYRQRASAGLIVAEGT
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCEEEECCC
HISPTAKGYAGAPGIYSEEQVRAWSEVTGAVHQDGGKIALQLWHTGRISHRSLQPNGDAP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCC
VGPSAIQADSRTNIRAADGSLVREQCDTPRALEIEEIEDIIEDYRRAADNARRAGFDMVE
CCCHHCCCCCCCCEEECCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
IHGAHGYLIDQFLSPAANVRTDQYGGSVENRARFLLEVVDAVVAEWDADHVGIRISPLGI
EECCCCHHHHHHHCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCE
FNGVSNTDQLDMALYLAEQLAKRKLAFLHISEPDWAGGPTLDDGFRAELRQRYPGVIIGA
ECCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCEEEEC
GGYSAEKAETLLKKGFIDAAAFGRSYIANPDLVERLKQNAPLNPPKPDTFYGGGAEGYTD
CCCCHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
YPTL
CCCC
>Mature Secondary Structure
MNADKLLTPLTMGAVALSNRVVMAPLTRLRNIEPGDVPGPLAKEYYRQRASAGLIVAEGT
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCEEEECCC
HISPTAKGYAGAPGIYSEEQVRAWSEVTGAVHQDGGKIALQLWHTGRISHRSLQPNGDAP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCC
VGPSAIQADSRTNIRAADGSLVREQCDTPRALEIEEIEDIIEDYRRAADNARRAGFDMVE
CCCHHCCCCCCCCEEECCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
IHGAHGYLIDQFLSPAANVRTDQYGGSVENRARFLLEVVDAVVAEWDADHVGIRISPLGI
EECCCCHHHHHHHCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCE
FNGVSNTDQLDMALYLAEQLAKRKLAFLHISEPDWAGGPTLDDGFRAELRQRYPGVIIGA
ECCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCEEEEC
GGYSAEKAETLLKKGFIDAAAFGRSYIANPDLVERLKQNAPLNPPKPDTFYGGGAEGYTD
CCCCHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
YPTL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]