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The map label for this gene is ptrB [C]

Identifier: 34498957

GI number: 34498957

Start: 3796284

End: 3798353

Strand: Direct

Name: ptrB [C]

Synonym: CV_3502

Alternate gene names: 34498957

Gene position: 3796284-3798353 (Clockwise)

Preceding gene: 34498956

Following gene: 34498958

Centisome position: 79.9

GC content: 67.49

Gene sequence:

>2070_bases
ATGAAGACCAGACTGCTCGTCTCCGCCGCCCTGCTGCTGGCCGCGCAGAGCCATGCGGCGGACGATCCCTACCTGTGGCT
GGAAAACATCCAGGGCAAGGATGCGCTGCGATGGGTGAAGCAGCAGAACGAGCGCACCAGCAAGGAACTGGAGAGCCGCA
AAGGATTCCCGGCGCTGAAGCGCGACATCCTGTCCGTGCTGGACTCCAGCGCCCGCATCCCCGCCATCGAAAAAATAGGC
GACCGCTACTACAACTTCTGGCGCGACGCCGCACACCCGCGCGGCCTGTGGCGCAGCGCCACGCTGGACGAATACCGCAA
GGCCGACCCCGCCTGGGAAACCGTGCTGGATCTGGACAAGCTGGCCCGCGATGAAAAGGAAAACTGGGTGTGGCAAGGCG
CCGACTGCGTCAAGCCCAGGCAGGACCGCTGCCTGATCCGGCTGAGCCGCGGCGGCGGCGACGCGGCGGTGGTGCGCGAA
TTCGACCTGCCGGCCAAGCGCTTCCTCGCCGACGGCTTCGTCGTCCCGGAAGCCAAGAGCGAAGTCAACTGGGCCGACCG
CGACACGCTGTACGTCGCCACCGATTTCGGCCAGGGCACGCTGACCGACTCCGGCTACCCCCGCATCGTCAAGGAATGGA
AGCGCGGCCAGCCGCTGGCCGCCGCCCCGACGCTGTTCGAGGGCAAGCAGACCGACATCTCGGTCAGCGCCGACGGCATC
GACACCGCCGGCCACCATTACGACGTGATCAGCCAGGGCACCAGCTTTTTCACCAACGACGCCTATCTGAAGCGGGACGG
CAAGTGGCTGAAGCTGGACAAGCCCGGCCACGTCGAAGCGTCCTTTCACGGCGCCTGGCTGCTGCTGCGGCCGCGCCAGG
ACTGGAGCGTCGGCGGCCATGACTGGAAGGCCGGCAGCCTGCTGGCGGTCAAGGTGGACGATTACCTGCAGGGCAAGCGC
GACTTCGCCGCCTTGTTCCAGCCCACTGCGTCCACCTCGCTGGGCGGCAGCGCCGGCACCCGCGACGCGCTGATCCTGAC
CGTGCTCGACAACGTCAAAAGCCGGCTGGTCGAATGGAAAGTGGCGGACGGCAAATGGCAGTCGCGCCCGGTGGCCGCGC
CAGGCTACGGCAGCATCAGCGTCAGCGCGGTGGACAGCGACGCCTCCGACGACTACTTCTTCACCTTCACCGACTTCCTG
ACGCCGACCTCGCTGTCGCTGGCCCACGCCGGCAGCGACGCGCGCGAGCCGCTGAAACAGCAGCCGGCCTTCTTCGACGC
CAAGCCCTACCGCATCCAGCAATTCTTCGCCCGCTCCGAGGACGGCACCCGCGTGCCTTACTTCGTGGTGGCGCGCAAGG
ATTTGAAGCTGAACGGCAAGAACCCGACGCTGCTCTACGGCTACGGCGGCTTCGAAGTGTCGCTGACGCCCTATTACAGC
GGCACCCTGGGCAAGGCCTGGCTGGAGCGCGGCGGCGTCTACGTGCTGGGCAACATCCGCGGCGGCGGCGAATACGGCCC
AGCCTGGCACGAGGCCGCGCGCAAGGAACACCGCCAGCGCGCCTACGACGACTTTTCGTCGATCGCCAAGGATCTGGCGC
GGCGCGGCATCGCCGACGCCCGCCATCTCGGCATCAAGGGCGGCAGCAACGGCGGCCTGTTGATGGGCGTGATGCTGACC
CAGTACCCGCAGCTGTTCCACGCCGTCGTCTGCCAGGTGCCGCTTCTGGACATGCGCCGCTACAACAAGCTGCTGGCCGG
CGCCAGCTGGATGGACGAGTACGGCGATCCCGACAAGCCCGCCGACTGGGCCTACATCAAGCGCTACTCGCCGTATCAGA
ACATCCGGCCCGGCGCCAAGTATCCGGCCACATTGTTCGCCACCTCCACGCTGGACGACCGCGTCCACCCCGGCCACGCC
CGCAAGATGTACGCGGCGATGAAGGAAATCGGCGCCGACGTCCGCTACTACGAGCGCACCGAGGGCGGCCACGGCGCAGC
CGCCAACCACGACGAGGAGGCCCGCATCGCCGCGATGGAGTACGCCTTCCTGTGGAGCCAGCTGCGCTGA

Upstream 100 bases:

>100_bases
CGAGATACAGTCTGGGATTGGCTGCCGGCCCGCCGCGACAACAAGGAGCCCTGCACGCCGGCCGCGCCGGCGGCAAGGGC
CCCGCACTTAGGAGAACATC

Downstream 100 bases:

>100_bases
TCGCGGACGGCCCCGCCCAGGGCCTTCCGGCTTCCTCCGTTTGACAAAACGCCGCTTCCGGCCGCCATCTCGGCTGGGAG
CGGCCCGCCTCCCTTCCCTG

Product: prolyl oligopeptidase family protein

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 689; Mature: 689

Protein sequence:

>689_residues
MKTRLLVSAALLLAAQSHAADDPYLWLENIQGKDALRWVKQQNERTSKELESRKGFPALKRDILSVLDSSARIPAIEKIG
DRYYNFWRDAAHPRGLWRSATLDEYRKADPAWETVLDLDKLARDEKENWVWQGADCVKPRQDRCLIRLSRGGGDAAVVRE
FDLPAKRFLADGFVVPEAKSEVNWADRDTLYVATDFGQGTLTDSGYPRIVKEWKRGQPLAAAPTLFEGKQTDISVSADGI
DTAGHHYDVISQGTSFFTNDAYLKRDGKWLKLDKPGHVEASFHGAWLLLRPRQDWSVGGHDWKAGSLLAVKVDDYLQGKR
DFAALFQPTASTSLGGSAGTRDALILTVLDNVKSRLVEWKVADGKWQSRPVAAPGYGSISVSAVDSDASDDYFFTFTDFL
TPTSLSLAHAGSDAREPLKQQPAFFDAKPYRIQQFFARSEDGTRVPYFVVARKDLKLNGKNPTLLYGYGGFEVSLTPYYS
GTLGKAWLERGGVYVLGNIRGGGEYGPAWHEAARKEHRQRAYDDFSSIAKDLARRGIADARHLGIKGGSNGGLLMGVMLT
QYPQLFHAVVCQVPLLDMRRYNKLLAGASWMDEYGDPDKPADWAYIKRYSPYQNIRPGAKYPATLFATSTLDDRVHPGHA
RKMYAAMKEIGADVRYYERTEGGHGAAANHDEEARIAAMEYAFLWSQLR

Sequences:

>Translated_689_residues
MKTRLLVSAALLLAAQSHAADDPYLWLENIQGKDALRWVKQQNERTSKELESRKGFPALKRDILSVLDSSARIPAIEKIG
DRYYNFWRDAAHPRGLWRSATLDEYRKADPAWETVLDLDKLARDEKENWVWQGADCVKPRQDRCLIRLSRGGGDAAVVRE
FDLPAKRFLADGFVVPEAKSEVNWADRDTLYVATDFGQGTLTDSGYPRIVKEWKRGQPLAAAPTLFEGKQTDISVSADGI
DTAGHHYDVISQGTSFFTNDAYLKRDGKWLKLDKPGHVEASFHGAWLLLRPRQDWSVGGHDWKAGSLLAVKVDDYLQGKR
DFAALFQPTASTSLGGSAGTRDALILTVLDNVKSRLVEWKVADGKWQSRPVAAPGYGSISVSAVDSDASDDYFFTFTDFL
TPTSLSLAHAGSDAREPLKQQPAFFDAKPYRIQQFFARSEDGTRVPYFVVARKDLKLNGKNPTLLYGYGGFEVSLTPYYS
GTLGKAWLERGGVYVLGNIRGGGEYGPAWHEAARKEHRQRAYDDFSSIAKDLARRGIADARHLGIKGGSNGGLLMGVMLT
QYPQLFHAVVCQVPLLDMRRYNKLLAGASWMDEYGDPDKPADWAYIKRYSPYQNIRPGAKYPATLFATSTLDDRVHPGHA
RKMYAAMKEIGADVRYYERTEGGHGAAANHDEEARIAAMEYAFLWSQLR
>Mature_689_residues
MKTRLLVSAALLLAAQSHAADDPYLWLENIQGKDALRWVKQQNERTSKELESRKGFPALKRDILSVLDSSARIPAIEKIG
DRYYNFWRDAAHPRGLWRSATLDEYRKADPAWETVLDLDKLARDEKENWVWQGADCVKPRQDRCLIRLSRGGGDAAVVRE
FDLPAKRFLADGFVVPEAKSEVNWADRDTLYVATDFGQGTLTDSGYPRIVKEWKRGQPLAAAPTLFEGKQTDISVSADGI
DTAGHHYDVISQGTSFFTNDAYLKRDGKWLKLDKPGHVEASFHGAWLLLRPRQDWSVGGHDWKAGSLLAVKVDDYLQGKR
DFAALFQPTASTSLGGSAGTRDALILTVLDNVKSRLVEWKVADGKWQSRPVAAPGYGSISVSAVDSDASDDYFFTFTDFL
TPTSLSLAHAGSDAREPLKQQPAFFDAKPYRIQQFFARSEDGTRVPYFVVARKDLKLNGKNPTLLYGYGGFEVSLTPYYS
GTLGKAWLERGGVYVLGNIRGGGEYGPAWHEAARKEHRQRAYDDFSSIAKDLARRGIADARHLGIKGGSNGGLLMGVMLT
QYPQLFHAVVCQVPLLDMRRYNKLLAGASWMDEYGDPDKPADWAYIKRYSPYQNIRPGAKYPATLFATSTLDDRVHPGHA
RKMYAAMKEIGADVRYYERTEGGHGAAANHDEEARIAAMEYAFLWSQLR

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=339, Percent_Identity=35.1032448377581, Blast_Score=199, Evalue=6e-51,
Organism=Homo sapiens, GI284172438, Length=215, Percent_Identity=28.8372093023256, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI284172431, Length=215, Percent_Identity=28.8372093023256, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI284172420, Length=215, Percent_Identity=28.8372093023256, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI284172413, Length=215, Percent_Identity=28.8372093023256, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI70778815, Length=215, Percent_Identity=28.8372093023256, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI108860686, Length=215, Percent_Identity=28.8372093023256, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI108860692, Length=202, Percent_Identity=28.7128712871287, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1788150, Length=299, Percent_Identity=31.438127090301, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI221510989, Length=308, Percent_Identity=35.7142857142857, Blast_Score=189, Evalue=4e-48,
Organism=Drosophila melanogaster, GI24583414, Length=368, Percent_Identity=33.1521739130435, Blast_Score=187, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 76984; Mature: 76984

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRLLVSAALLLAAQSHAADDPYLWLENIQGKDALRWVKQQNERTSKELESRKGFPALK
CCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
RDILSVLDSSARIPAIEKIGDRYYNFWRDAAHPRGLWRSATLDEYRKADPAWETVLDLDK
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHH
LARDEKENWVWQGADCVKPRQDRCLIRLSRGGGDAAVVREFDLPAKRFLADGFVVPEAKS
HHCCCCCCCEECCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCHHHHHHCCCCCCCCCC
EVNWADRDTLYVATDFGQGTLTDSGYPRIVKEWKRGQPLAAAPTLFEGKQTDISVSADGI
CCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCC
DTAGHHYDVISQGTSFFTNDAYLKRDGKWLKLDKPGHVEASFHGAWLLLRPRQDWSVGGH
CCCCCCCHHHHCCCCEECCCCEEECCCCEEEECCCCCEEEEECCEEEEEECCCCCCCCCC
DWKAGSLLAVKVDDYLQGKRDFAALFQPTASTSLGGSAGTRDALILTVLDNVKSRLVEWK
CCCCCCEEEEEEHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHEEEE
VADGKWQSRPVAAPGYGSISVSAVDSDASDDYFFTFTDFLTPTSLSLAHAGSDAREPLKQ
ECCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEHHCCCCCCCHHHCCCHHHHHHHH
QPAFFDAKPYRIQQFFARSEDGTRVPYFVVARKDLKLNGKNPTLLYGYGGFEVSLTPYYS
CCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEECEEECCCCCEEEEEECCEEEEEECCCC
GTLGKAWLERGGVYVLGNIRGGGEYGPAWHEAARKEHRQRAYDDFSSIAKDLARRGIADA
CCHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
RHLGIKGGSNGGLLMGVMLTQYPQLFHAVVCQVPLLDMRRYNKLLAGASWMDEYGDPDKP
HHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHCCCCCCC
ADWAYIKRYSPYQNIRPGAKYPATLFATSTLDDRVHPGHARKMYAAMKEIGADVRYYERT
CCCHHHCCCCCHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEC
EGGHGAAANHDEEARIAAMEYAFLWSQLR
CCCCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTRLLVSAALLLAAQSHAADDPYLWLENIQGKDALRWVKQQNERTSKELESRKGFPALK
CCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
RDILSVLDSSARIPAIEKIGDRYYNFWRDAAHPRGLWRSATLDEYRKADPAWETVLDLDK
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHH
LARDEKENWVWQGADCVKPRQDRCLIRLSRGGGDAAVVREFDLPAKRFLADGFVVPEAKS
HHCCCCCCCEECCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCHHHHHHCCCCCCCCCC
EVNWADRDTLYVATDFGQGTLTDSGYPRIVKEWKRGQPLAAAPTLFEGKQTDISVSADGI
CCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCC
DTAGHHYDVISQGTSFFTNDAYLKRDGKWLKLDKPGHVEASFHGAWLLLRPRQDWSVGGH
CCCCCCCHHHHCCCCEECCCCEEECCCCEEEECCCCCEEEEECCEEEEEECCCCCCCCCC
DWKAGSLLAVKVDDYLQGKRDFAALFQPTASTSLGGSAGTRDALILTVLDNVKSRLVEWK
CCCCCCEEEEEEHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHEEEE
VADGKWQSRPVAAPGYGSISVSAVDSDASDDYFFTFTDFLTPTSLSLAHAGSDAREPLKQ
ECCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEHHCCCCCCCHHHCCCHHHHHHHH
QPAFFDAKPYRIQQFFARSEDGTRVPYFVVARKDLKLNGKNPTLLYGYGGFEVSLTPYYS
CCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEECEEECCCCCEEEEEECCEEEEEECCCC
GTLGKAWLERGGVYVLGNIRGGGEYGPAWHEAARKEHRQRAYDDFSSIAKDLARRGIADA
CCHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
RHLGIKGGSNGGLLMGVMLTQYPQLFHAVVCQVPLLDMRRYNKLLAGASWMDEYGDPDKP
HHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHCCCCCCC
ADWAYIKRYSPYQNIRPGAKYPATLFATSTLDDRVHPGHARKMYAAMKEIGADVRYYERT
CCCHHHCCCCCHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEC
EGGHGAAANHDEEARIAAMEYAFLWSQLR
CCCCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.23 {tosyl-Arg} 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.47 {tosyl-Lys-methyl} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 80 {acetyl-tyrosine} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]