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The map label for this gene is hmpA

Identifier: 34498943

GI number: 34498943

Start: 3781273

End: 3782487

Strand: Reverse

Name: hmpA

Synonym: CV_3488

Alternate gene names: 34498943

Gene position: 3782487-3781273 (Counterclockwise)

Preceding gene: 34498944

Following gene: 34498942

Centisome position: 79.61

GC content: 68.15

Gene sequence:

>1215_bases
ATGCTGAGCGAACAAACCCGATCCCTGGTCAAGGCCACCGTCCCGGTGCTGCAGCAGCACGGCGTGGCGCTGACCAGCCA
TTTCTACCGCCGCATGTTCGAGCACAATCCGGAACTGAAAAACGTGTTCAACCAGGGGCACCAGCATTCCGGCCAGCAGC
AGCAGGCGCTGGCGATGGCGGTGCTGGCCTATGCCCGGCACATCGACGACCCGTCGCCGCTGCTGCCGGTGCTGACCCGC
GTCGCGCACAAGCACGTCAGCCTGGGCATACGCGCCGAGCACTACCCGATTGTCGGCAAGCACTTGCTGGCGTCGATCCG
CGAGTTGCTGGGCGAGGCGGCCGGCGACGATCTGATCCAGGCCTGGGCCGAGGCCTACGGTTTGCTGGCGGATACGCTGA
TCGGCATCGAAAACGGCATGTACGGCGACGCCACCAGCGCCGACGGCGGCTGGAGCGGCTGGCGGCCGTTCCGCGTGGCG
AAGAAGGAAATCGAGAGCGAGGAGATCGCGTCGTTTTACCTGGAGCCCAGCGACGGCGGCGCGCTGCCGGCGTTCAAGCC
CGGCCAGTACGTGTCGGTGAAGCGCTTCGTCGCCGAGTGGGGGCTGAGCCAGCCGCGCCAGTACAGCCTGTCCGACGCGC
CCAACGGCGAATACCTGCGCATCTCGGTCAAGCGCGAAGACGCGGCGCAGGGCAAGCCGGCGGGGCGAGTATCCAATCTG
TTGCACCGCGAGGTGCAGGTCGGCGATGTGCTGGAGCTGAGCGCGCCGCAGGGCGATTTCTTCCTGCATGAGGAACGCGA
CGGTCCGGCGGTGCTGATCAGCGCCGGCGTCGGCCAGACGCCGATGCAGGCGATGCTGGGCCAGTTGCTCAAGCGCGGCG
GCCGCGAAGTGCGCTTCCTGCACGCGGCCCGTCACGGCGGCGCGCATGCGATGGGCGCCAAAGTGCGCCAGCTGGCCGAT
CGCCATCCGCAACTGAAGGTCCACGTTTGCTACGAGACGCCGCGCGCGCAGGATGCGATCGGCGTCGACTACCAGGCTGC
GGGGCGGCTGAACCTGGCGGATGTGAAGGGCATCGCGCTGTTGCCGGACGCCGACTACTATCTGTGCGGCCCGCTGGGCT
TCATGCGCGCGCAGCGCGACAGCCTGCGCGGCCTGGGCGTGGCCGCCGACCGCATCCATTACGAAGTGTTCGGCTCCCAT
CCCGGCGACGATTGA

Upstream 100 bases:

>100_bases
TAAACATGTATTATTTGAACATCTTTTAAGAATCCCCGGTCGGTAGGGGATTTTTTTCCGGCAATAAAGATGTATTTTTT
AAATATCTTAAGGAGTTGGT

Downstream 100 bases:

>100_bases
GGCCGCCGGCCAGACGCGGTATGCTGCCGCCGATTCCCTGCACTGATTAGAGCGCCATGCAACTGACCCGATTCACCGAC
CTAGGCCTGCGCGTGCTGAT

Product: nitric oxide dioxygenase

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MLSEQTRSLVKATVPVLQQHGVALTSHFYRRMFEHNPELKNVFNQGHQHSGQQQQALAMAVLAYARHIDDPSPLLPVLTR
VAHKHVSLGIRAEHYPIVGKHLLASIRELLGEAAGDDLIQAWAEAYGLLADTLIGIENGMYGDATSADGGWSGWRPFRVA
KKEIESEEIASFYLEPSDGGALPAFKPGQYVSVKRFVAEWGLSQPRQYSLSDAPNGEYLRISVKREDAAQGKPAGRVSNL
LHREVQVGDVLELSAPQGDFFLHEERDGPAVLISAGVGQTPMQAMLGQLLKRGGREVRFLHAARHGGAHAMGAKVRQLAD
RHPQLKVHVCYETPRAQDAIGVDYQAAGRLNLADVKGIALLPDADYYLCGPLGFMRAQRDSLRGLGVAADRIHYEVFGSH
PGDD

Sequences:

>Translated_404_residues
MLSEQTRSLVKATVPVLQQHGVALTSHFYRRMFEHNPELKNVFNQGHQHSGQQQQALAMAVLAYARHIDDPSPLLPVLTR
VAHKHVSLGIRAEHYPIVGKHLLASIRELLGEAAGDDLIQAWAEAYGLLADTLIGIENGMYGDATSADGGWSGWRPFRVA
KKEIESEEIASFYLEPSDGGALPAFKPGQYVSVKRFVAEWGLSQPRQYSLSDAPNGEYLRISVKREDAAQGKPAGRVSNL
LHREVQVGDVLELSAPQGDFFLHEERDGPAVLISAGVGQTPMQAMLGQLLKRGGREVRFLHAARHGGAHAMGAKVRQLAD
RHPQLKVHVCYETPRAQDAIGVDYQAAGRLNLADVKGIALLPDADYYLCGPLGFMRAQRDSLRGLGVAADRIHYEVFGSH
PGDD
>Mature_404_residues
MLSEQTRSLVKATVPVLQQHGVALTSHFYRRMFEHNPELKNVFNQGHQHSGQQQQALAMAVLAYARHIDDPSPLLPVLTR
VAHKHVSLGIRAEHYPIVGKHLLASIRELLGEAAGDDLIQAWAEAYGLLADTLIGIENGMYGDATSADGGWSGWRPFRVA
KKEIESEEIASFYLEPSDGGALPAFKPGQYVSVKRFVAEWGLSQPRQYSLSDAPNGEYLRISVKREDAAQGKPAGRVSNL
LHREVQVGDVLELSAPQGDFFLHEERDGPAVLISAGVGQTPMQAMLGQLLKRGGREVRFLHAARHGGAHAMGAKVRQLAD
RHPQLKVHVCYETPRAQDAIGVDYQAAGRLNLADVKGIALLPDADYYLCGPLGFMRAQRDSLRGLGVAADRIHYEVFGSH
PGDD

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain

Homologues:

Organism=Escherichia coli, GI1788903, Length=401, Percent_Identity=44.139650872818, Blast_Score=338, Evalue=3e-94,
Organism=Escherichia coli, GI1788104, Length=256, Percent_Identity=28.515625, Blast_Score=75, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6321673, Length=410, Percent_Identity=39.2682926829268, Blast_Score=254, Evalue=1e-68,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): HMP_CHRVO (Q7NSD8)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903158.1
- ProteinModelPortal:   Q7NSD8
- SMR:   Q7NSD8
- GeneID:   2549191
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3488
- NMPDR:   fig|243365.1.peg.3488
- HOGENOM:   HBG623097
- OMA:   QIGHKHR
- PhylomeDB:   Q7NSD8
- ProtClustDB:   PRK13289
- BioCyc:   CVIO243365:CV_3488-MONOMER
- BRENDA:   1.14.12.17
- HAMAP:   MF_01252
- InterPro:   IPR017927
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938
- Gene3D:   G3DSA:1.10.490.10

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1; SSF46458 Globin_like; SSF63380 Riboflavin_synthase_like_b-brl

EC number: =1.14.12.17

Molecular weight: Translated: 44216; Mature: 44216

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS51384 FAD_FR; PS01033 GLOBIN

Important sites: ACT_SITE 95-95 ACT_SITE 137-137 BINDING 190-190

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSEQTRSLVKATVPVLQQHGVALTSHFYRRMFEHNPELKNVFNQGHQHSGQQQQALAMA
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHH
VLAYARHIDDPSPLLPVLTRVAHKHVSLGIRAEHYPIVGKHLLASIRELLGEAAGDDLIQ
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHHHHCCHHHHH
AWAEAYGLLADTLIGIENGMYGDATSADGGWSGWRPFRVAKKEIESEEIASFYLEPSDGG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHEEECCCCCC
ALPAFKPGQYVSVKRFVAEWGLSQPRQYSLSDAPNGEYLRISVKREDAAQGKPAGRVSNL
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHH
LHREVQVGDVLELSAPQGDFFLHEERDGPAVLISAGVGQTPMQAMLGQLLKRGGREVRFL
HHHHHCCCCEEEECCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEE
HAARHGGAHAMGAKVRQLADRHPQLKVHVCYETPRAQDAIGVDYQAAGRLNLADVKGIAL
EHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHCCCCCEEECCCCEEE
LPDADYYLCGPLGFMRAQRDSLRGLGVAADRIHYEVFGSHPGDD
CCCCCEEEECCHHHHHHHHHHHCCCCCCHHHEEEEEECCCCCCC
>Mature Secondary Structure
MLSEQTRSLVKATVPVLQQHGVALTSHFYRRMFEHNPELKNVFNQGHQHSGQQQQALAMA
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHH
VLAYARHIDDPSPLLPVLTRVAHKHVSLGIRAEHYPIVGKHLLASIRELLGEAAGDDLIQ
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHHHHCCHHHHH
AWAEAYGLLADTLIGIENGMYGDATSADGGWSGWRPFRVAKKEIESEEIASFYLEPSDGG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHEEECCCCCC
ALPAFKPGQYVSVKRFVAEWGLSQPRQYSLSDAPNGEYLRISVKREDAAQGKPAGRVSNL
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHH
LHREVQVGDVLELSAPQGDFFLHEERDGPAVLISAGVGQTPMQAMLGQLLKRGGREVRFL
HHHHHCCCCEEEECCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEE
HAARHGGAHAMGAKVRQLADRHPQLKVHVCYETPRAQDAIGVDYQAAGRLNLADVKGIAL
EHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHCCCCCEEECCCCEEE
LPDADYYLCGPLGFMRAQRDSLRGLGVAADRIHYEVFGSHPGDD
CCCCCEEEECCHHHHHHHHHHHCCCCCCHHHEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782