Click here to switch to the map view.

The map label for this gene is yafC [C]

Identifier: 34498937

GI number: 34498937

Start: 3777863

End: 3778810

Strand: Reverse

Name: yafC [C]

Synonym: CV_3482

Alternate gene names: 34498937

Gene position: 3778810-3777863 (Counterclockwise)

Preceding gene: 34498940

Following gene: 34498936

Centisome position: 79.54

GC content: 68.35

Gene sequence:

>948_bases
ATGGAGCATTTCGCCGCGATACCGATATTCGTCGCCGTGGTCGAGGCCGGCAGTTTCTCTGCCGCCGCCGAACGGCTGGG
GCTGACCAAATCCGCGGTCAGCAAGCGGGTGCAACAATTGGAGGCGGGCTTGAACGTGAGGCTGCTGCAGCGGACCACCC
GCAGTCTGAGCCTGACCGAGGCCGGCGAGCGCTATTACGACCGCGCCCGCGGCGCGCTCGCGCTGGCCCGCGAGGCGGAG
GAGGTGGCGGCCGGGCTGCAGGGCCGGCCGCAGGGTTTGCTGCGGGTTGCCGCGCCGATGGCTTTCGGCCGGCTGCACCT
GTCGCCGCTGGCGGCGGAGTTCCTGCGCCGCTATCCGGACATCCAGCTGGACTTGGCGATGGACGACAGGATGGTAGACC
TGGTGGAGGGCGGCTACGACCTGGCGGTGCGGATCGGCAATCTGCCGGACAACCGCATGGTGGCGCGCCGCCTCGCCCCG
TGCCGGGTAGCGGTGTGCGCGTCGCCGGACTACCTGGCGGCGCATGGAAGGCCGGCGCATCCATCCGAGCTGGCGCGGCA
CAACTGCATCCATTATTCATACTATCGCGGCGGCGCTTACTGGACTTTCCAGGGGCCGGACGGCGAGATCCGCGTGCAGC
CTCGCGGCAATTACCAGGTCAACAACAGCGAGGCGCGGCGCGACGCGCTGCTGGCCGGCCTGGGCATCTGTGAAATGCCC
ACCTTCATCGTCGGGCCGGACTTGGCCGCCGGCCGGCTGGAAGCGGTGCTGACCGATTACCCGCTGCCGCCGCACGCTAT
CCACGTGGTGTTCCCGCAACGGCGGCTGCCGGCCAAGGCTCGGGCCTTCATCGACTTCCTTGACGAAAAACTGGGCGGCG
ACAGGCCGTTGTGGGATAGCGACCTGCCCCCATCTGTGCTGTCATCTCAATATGGGGAGCGCCCATGA

Upstream 100 bases:

>100_bases
TGAACACGGCGAGGCCGGATGTCAGAACTGTACATTGTGTCTTTCTGGCTGATAATCTGTATTTTTGGAAACTGATTGCG
GATGGAAAGTGGACAATAAG

Downstream 100 bases:

>100_bases
GCAAACGTTATCGGACGGCCTTGGTCTGGCTGCGCCGCGACCTGCGGCTGGACGACCACGCCGCTTTTTACCATGCGCTG
AAGCACAGCGAGGCCGTGCT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MEHFAAIPIFVAVVEAGSFSAAAERLGLTKSAVSKRVQQLEAGLNVRLLQRTTRSLSLTEAGERYYDRARGALALAREAE
EVAAGLQGRPQGLLRVAAPMAFGRLHLSPLAAEFLRRYPDIQLDLAMDDRMVDLVEGGYDLAVRIGNLPDNRMVARRLAP
CRVAVCASPDYLAAHGRPAHPSELARHNCIHYSYYRGGAYWTFQGPDGEIRVQPRGNYQVNNSEARRDALLAGLGICEMP
TFIVGPDLAAGRLEAVLTDYPLPPHAIHVVFPQRRLPAKARAFIDFLDEKLGGDRPLWDSDLPPSVLSSQYGERP

Sequences:

>Translated_315_residues
MEHFAAIPIFVAVVEAGSFSAAAERLGLTKSAVSKRVQQLEAGLNVRLLQRTTRSLSLTEAGERYYDRARGALALAREAE
EVAAGLQGRPQGLLRVAAPMAFGRLHLSPLAAEFLRRYPDIQLDLAMDDRMVDLVEGGYDLAVRIGNLPDNRMVARRLAP
CRVAVCASPDYLAAHGRPAHPSELARHNCIHYSYYRGGAYWTFQGPDGEIRVQPRGNYQVNNSEARRDALLAGLGICEMP
TFIVGPDLAAGRLEAVLTDYPLPPHAIHVVFPQRRLPAKARAFIDFLDEKLGGDRPLWDSDLPPSVLSSQYGERP
>Mature_315_residues
MEHFAAIPIFVAVVEAGSFSAAAERLGLTKSAVSKRVQQLEAGLNVRLLQRTTRSLSLTEAGERYYDRARGALALAREAE
EVAAGLQGRPQGLLRVAAPMAFGRLHLSPLAAEFLRRYPDIQLDLAMDDRMVDLVEGGYDLAVRIGNLPDNRMVARRLAP
CRVAVCASPDYLAAHGRPAHPSELARHNCIHYSYYRGGAYWTFQGPDGEIRVQPRGNYQVNNSEARRDALLAGLGICEMP
TFIVGPDLAAGRLEAVLTDYPLPPHAIHVVFPQRRLPAKARAFIDFLDEKLGGDRPLWDSDLPPSVLSSQYGERP

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=285, Percent_Identity=36.4912280701754, Blast_Score=177, Evalue=6e-46,
Organism=Escherichia coli, GI1787128, Length=290, Percent_Identity=34.4827586206897, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=36.551724137931, Blast_Score=167, Evalue=7e-43,
Organism=Escherichia coli, GI1789639, Length=291, Percent_Identity=32.6460481099656, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1789440, Length=283, Percent_Identity=31.8021201413428, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI87081978, Length=290, Percent_Identity=32.7586206896552, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1787589, Length=293, Percent_Identity=32.0819112627986, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=25.5102040816327, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1789173, Length=268, Percent_Identity=29.4776119402985, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788748, Length=175, Percent_Identity=34.8571428571429, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI157672245, Length=122, Percent_Identity=39.344262295082, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1786448, Length=270, Percent_Identity=25.9259259259259, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787806, Length=139, Percent_Identity=35.2517985611511, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786508, Length=123, Percent_Identity=30.8943089430894, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI145693105, Length=159, Percent_Identity=27.6729559748428, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787601, Length=210, Percent_Identity=25.7142857142857, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082132, Length=253, Percent_Identity=25.6916996047431, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787879, Length=240, Percent_Identity=27.9166666666667, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786713, Length=141, Percent_Identity=35.4609929078014, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788481, Length=292, Percent_Identity=26.3698630136986, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789492, Length=292, Percent_Identity=30.1369863013699, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34632; Mature: 34632

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHFAAIPIFVAVVEAGSFSAAAERLGLTKSAVSKRVQQLEAGLNVRLLQRTTRSLSLTE
CCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEHHHHHHHCCHHH
AGERYYDRARGALALAREAEEVAAGLQGRPQGLLRVAAPMAFGRLHLSPLAAEFLRRYPD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IQLDLAMDDRMVDLVEGGYDLAVRIGNLPDNRMVARRLAPCRVAVCASPDYLAAHGRPAH
CEEEEECCCHHHHHHCCCEEEEEEECCCCCHHHHHHHCCCCEEEEECCCCEEECCCCCCC
PSELARHNCIHYSYYRGGAYWTFQGPDGEIRVQPRGNYQVNNSEARRDALLAGLGICEMP
HHHHHHHCCEEEEEECCCEEEEEECCCCEEEEEECCCEEECCCHHHHHHHHHCCCHHHCC
TFIVGPDLAAGRLEAVLTDYPLPPHAIHVVFPQRRLPAKARAFIDFLDEKLGGDRPLWDS
CEEECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCC
DLPPSVLSSQYGERP
CCCHHHHHHHCCCCC
>Mature Secondary Structure
MEHFAAIPIFVAVVEAGSFSAAAERLGLTKSAVSKRVQQLEAGLNVRLLQRTTRSLSLTE
CCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEHHHHHHHCCHHH
AGERYYDRARGALALAREAEEVAAGLQGRPQGLLRVAAPMAFGRLHLSPLAAEFLRRYPD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IQLDLAMDDRMVDLVEGGYDLAVRIGNLPDNRMVARRLAPCRVAVCASPDYLAAHGRPAH
CEEEEECCCHHHHHHCCCEEEEEEECCCCCHHHHHHHCCCCEEEEECCCCEEECCCCCCC
PSELARHNCIHYSYYRGGAYWTFQGPDGEIRVQPRGNYQVNNSEARRDALLAGLGICEMP
HHHHHHHCCEEEEEECCCEEEEEECCCCEEEEEECCCEEECCCHHHHHHHHHCCCHHHCC
TFIVGPDLAAGRLEAVLTDYPLPPHAIHVVFPQRRLPAKARAFIDFLDEKLGGDRPLWDS
CEEECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCC
DLPPSVLSSQYGERP
CCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]