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The map label for this gene is smpB

Identifier: 34498919

GI number: 34498919

Start: 3758636

End: 3759091

Strand: Direct

Name: smpB

Synonym: CV_3464

Alternate gene names: 34498919

Gene position: 3758636-3759091 (Clockwise)

Preceding gene: 34498916

Following gene: 34498920

Centisome position: 79.11

GC content: 61.4

Gene sequence:

>456_bases
ATGAGCATCATCCAGAACCGCAAGGCTTTCCACGACTACTTCATCGAGGAAAAACTCGAGGCCGGGCTTGTGCTGGAAGG
GTGGGAAGTCAAGGCGATCCGCGCCGGCCGCGTACAGCTGAAGGAAAGCTACGTCGACTGGAAGAACGGCGCCTTCTGGC
TGATCGGCTGCCACATCACCCCGCTGCAGTCCGCCTCCACCCATGTCAAGCCGGACCCGGTGCGTCCGCGCAAGCTCCTG
ATGTCCCAGGCCGAGATCAACCGCTTCATCGGCAAGGTCGAACGCGCCGGCTACACCATGATGGCGCTGGACCTGCATTA
CACCAAGGGCAACGTCAAGGCCGAGGTCGGCCTGGCCAAGGGCAAGAAGCTGCACGACAAGCGCGACACTGAAAAAGACC
GCGAATGGCAACGCGAAAAACAGCGCGTGATGAAGAACCAGCGGGGCGCGGCATGA

Upstream 100 bases:

>100_bases
GCACGGCCAAGTCCTTTCACATCAAACCACTGAAAAGGCAGGATTTTGGTTGCGGGCCGGCTTCTGATACAATGTCGGAT
TCAAAGGAACTCACCGTCAC

Downstream 100 bases:

>100_bases
AGCGCGGCGCCGCCCTGCCCATCGCCCTGATCTGCCTGGGCAGCGTCTGGTTCCTGAAAAGCACCGCCCTGCTGCCCAAC
ACCTCCACCCTGCTCGCCCT

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 151; Mature: 150

Protein sequence:

>151_residues
MSIIQNRKAFHDYFIEEKLEAGLVLEGWEVKAIRAGRVQLKESYVDWKNGAFWLIGCHITPLQSASTHVKPDPVRPRKLL
MSQAEINRFIGKVERAGYTMMALDLHYTKGNVKAEVGLAKGKKLHDKRDTEKDREWQREKQRVMKNQRGAA

Sequences:

>Translated_151_residues
MSIIQNRKAFHDYFIEEKLEAGLVLEGWEVKAIRAGRVQLKESYVDWKNGAFWLIGCHITPLQSASTHVKPDPVRPRKLL
MSQAEINRFIGKVERAGYTMMALDLHYTKGNVKAEVGLAKGKKLHDKRDTEKDREWQREKQRVMKNQRGAA
>Mature_150_residues
SIIQNRKAFHDYFIEEKLEAGLVLEGWEVKAIRAGRVQLKESYVDWKNGAFWLIGCHITPLQSASTHVKPDPVRPRKLLM
SQAEINRFIGKVERAGYTMMALDLHYTKGNVKAEVGLAKGKKLHDKRDTEKDREWQREKQRVMKNQRGAA

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=145, Percent_Identity=51.7241379310345, Blast_Score=160, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_CHRVO (Q7NSG2)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903134.1
- ProteinModelPortal:   Q7NSG2
- SMR:   Q7NSG2
- GeneID:   2547642
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3464
- NMPDR:   fig|243365.1.peg.3464
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- PhylomeDB:   Q7NSG2
- ProtClustDB:   PRK05422
- BioCyc:   CVIO243365:CV_3464-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 17460; Mature: 17329

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIIQNRKAFHDYFIEEKLEAGLVLEGWEVKAIRAGRVQLKESYVDWKNGAFWLIGCHIT
CCCCHHHHHHHHHHHHHHHCCCEEEECCEEEEEECCHHHHHHHHCCCCCCEEEEEEEEEC
PLQSASTHVKPDPVRPRKLLMSQAEINRFIGKVERAGYTMMALDLHYTKGNVKAEVGLAK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCEEEEEECCC
GKKLHDKRDTEKDREWQREKQRVMKNQRGAA
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SIIQNRKAFHDYFIEEKLEAGLVLEGWEVKAIRAGRVQLKESYVDWKNGAFWLIGCHIT
CCCHHHHHHHHHHHHHHHCCCEEEECCEEEEEECCHHHHHHHHCCCCCCEEEEEEEEEC
PLQSASTHVKPDPVRPRKLLMSQAEINRFIGKVERAGYTMMALDLHYTKGNVKAEVGLAK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCEEEEEECCC
GKKLHDKRDTEKDREWQREKQRVMKNQRGAA
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782