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The map label for this gene is dosC [H]

Identifier: 34498916

GI number: 34498916

Start: 3756372

End: 3757634

Strand: Direct

Name: dosC [H]

Synonym: CV_3461

Alternate gene names: 34498916

Gene position: 3756372-3757634 (Clockwise)

Preceding gene: 34498915

Following gene: 34498919

Centisome position: 79.06

GC content: 65.0

Gene sequence:

>1263_bases
CTGCCCGCGCTTGACGGCGCCGACGAAGCCGCGAGCGACGCCTCCGGCAGCTGGCAGGCCTGGCAGCGCACCCTGGCTTT
CGCCCTCAAGCACGGACTGGAACCGCGCCTGTCCGACTATCCCGACCTGGTGGATGGACTGGAGTCGCTGCTGCAGGAGC
TGGAGGGCATCGCCGACGATCCCAGCCTGAACGTGTTCATGCCCAAGCTGCGGGCCTTCATGATCAAGCTGGAGCTGCAG
TCGCAGCAGGAAGGGCGCCTGGCCACCGGCCTCGCCAACCTGCTGAAGCTGATGCTGGACAACGTCGCCGAGCTGAACCG
CTCCGACGAATACCTGCTCGGCCAGGTCGGCTCCTTGCAGGAAATCCTGTCGCACGAGCCGCTGTCGATGCAGCAGATCT
ACCAGCTGGAAACCAGCCTGAAAGAGGTCATCCGCAAGCAGGGCATGTTGAAGAGCTCGCTGGACGAAGCCGCCAGCTCG
CTGCGCGCATTGCTGGACCGCTTCGTCAGCCGGCTGGCGCTGATGACCGACAGCACCGACGAATTCCACGGCAAGATCCA
GGCGCACAGCGAAAAGCTGAAACAGATGCCCAATGTGGACGAATTGGGCGGCATCATCGGCTCGCTGATGGAGGACACCT
CCATCATGCAACTGGACCTGACCCGTTCCCGCGACGAACTGGTCTCCGCCAAGGAACAGGTGGAGGCGGCCGAACACCGC
ATCCGCGAACTGGAAACCGCGCTGGAAGCCGCCAGCGCCAAGGTGAAGGAAGACCAGCTGACCGGCGCCTACAACCGCCG
CGGCCTGGCCGAATACTTCCAGCGCGAGATCGGCCGCGCCGAACGCACCGCCGCGCCGCTGTCCATCGCGCTGATCGACG
TCGACAACTTCAAGCAGCTGAACGACCGCTACGGCCACCTGGCCGGCGACGACGCGTTGAAATACCTGGTGGACACCATC
AAGCACAGCCTGCGCCCGGCCGACATCGTCGCCCGCTTCGGCGGCGAGGAGTTCGTGATCCTGATGCCGGACACGCCGGA
GACCGAGGCGGTGCAGACCGTGCAGCGTCTGCAGCGCGAACTGACCAAGACCTTCTTCCTGGCCAATAACGACAAACTGT
TGATCACCTTCAGCGCCGGCGTCGCCCGCTGGCATCTGGGCGAGCAGGAGTCCGACGTGCTGGAGCGCGCCGACCACGCG
ATGTACCAGGCCAAGATCAACGGCAAGAACCGCGTCTGCTCGGCCGAGGACATGCCCGGCTGA

Upstream 100 bases:

>100_bases
CTTCGGCAACCAGCCGGAGGAGCTGAACCAGAAGCTGGTCGCCCTGCTGCAGAACTGGAACGCCTCGCCGTCCAGCCGCG
ATCTCCCCCCGCCCGAGCCG

Downstream 100 bases:

>100_bases
CCAGCCCGTTGGCCAATAAAAAAAGGATGGCTTCCAGGCCATCCTTTTTTTGTTTCCACCCGCCGGACTCAGCCGCCCTT
TTTCATCCTCTTCCCGGCTT

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MPALDGADEAASDASGSWQAWQRTLAFALKHGLEPRLSDYPDLVDGLESLLQELEGIADDPSLNVFMPKLRAFMIKLELQ
SQQEGRLATGLANLLKLMLDNVAELNRSDEYLLGQVGSLQEILSHEPLSMQQIYQLETSLKEVIRKQGMLKSSLDEAASS
LRALLDRFVSRLALMTDSTDEFHGKIQAHSEKLKQMPNVDELGGIIGSLMEDTSIMQLDLTRSRDELVSAKEQVEAAEHR
IRELETALEAASAKVKEDQLTGAYNRRGLAEYFQREIGRAERTAAPLSIALIDVDNFKQLNDRYGHLAGDDALKYLVDTI
KHSLRPADIVARFGGEEFVILMPDTPETEAVQTVQRLQRELTKTFFLANNDKLLITFSAGVARWHLGEQESDVLERADHA
MYQAKINGKNRVCSAEDMPG

Sequences:

>Translated_420_residues
MPALDGADEAASDASGSWQAWQRTLAFALKHGLEPRLSDYPDLVDGLESLLQELEGIADDPSLNVFMPKLRAFMIKLELQ
SQQEGRLATGLANLLKLMLDNVAELNRSDEYLLGQVGSLQEILSHEPLSMQQIYQLETSLKEVIRKQGMLKSSLDEAASS
LRALLDRFVSRLALMTDSTDEFHGKIQAHSEKLKQMPNVDELGGIIGSLMEDTSIMQLDLTRSRDELVSAKEQVEAAEHR
IRELETALEAASAKVKEDQLTGAYNRRGLAEYFQREIGRAERTAAPLSIALIDVDNFKQLNDRYGHLAGDDALKYLVDTI
KHSLRPADIVARFGGEEFVILMPDTPETEAVQTVQRLQRELTKTFFLANNDKLLITFSAGVARWHLGEQESDVLERADHA
MYQAKINGKNRVCSAEDMPG
>Mature_419_residues
PALDGADEAASDASGSWQAWQRTLAFALKHGLEPRLSDYPDLVDGLESLLQELEGIADDPSLNVFMPKLRAFMIKLELQS
QQEGRLATGLANLLKLMLDNVAELNRSDEYLLGQVGSLQEILSHEPLSMQQIYQLETSLKEVIRKQGMLKSSLDEAASSL
RALLDRFVSRLALMTDSTDEFHGKIQAHSEKLKQMPNVDELGGIIGSLMEDTSIMQLDLTRSRDELVSAKEQVEAAEHRI
RELETALEAASAKVKEDQLTGAYNRRGLAEYFQREIGRAERTAAPLSIALIDVDNFKQLNDRYGHLAGDDALKYLVDTIK
HSLRPADIVARFGGEEFVILMPDTPETEAVQTVQRLQRELTKTFFLANNDKLLITFSAGVARWHLGEQESDVLERADHAM
YQAKINGKNRVCSAEDMPG

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=165, Percent_Identity=37.5757575757576, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1786584, Length=187, Percent_Identity=34.7593582887701, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI87081881, Length=176, Percent_Identity=36.9318181818182, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI87082007, Length=164, Percent_Identity=34.7560975609756, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787816, Length=157, Percent_Identity=36.9426751592357, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1787262, Length=174, Percent_Identity=30.4597701149425, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787802, Length=159, Percent_Identity=28.9308176100629, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081974, Length=154, Percent_Identity=30.5194805194805, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081977, Length=180, Percent_Identity=30.5555555555556, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788085, Length=201, Percent_Identity=27.363184079602, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787541, Length=157, Percent_Identity=29.9363057324841, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788381, Length=177, Percent_Identity=29.3785310734463, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 46837; Mature: 46705

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPALDGADEAASDASGSWQAWQRTLAFALKHGLEPRLSDYPDLVDGLESLLQELEGIADD
CCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
PSLNVFMPKLRAFMIKLELQSQQEGRLATGLANLLKLMLDNVAELNRSDEYLLGQVGSLQ
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCHHH
EILSHEPLSMQQIYQLETSLKEVIRKQGMLKSSLDEAASSLRALLDRFVSRLALMTDSTD
HHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EFHGKIQAHSEKLKQMPNVDELGGIIGSLMEDTSIMQLDLTRSRDELVSAKEQVEAAEHR
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHH
IRELETALEAASAKVKEDQLTGAYNRRGLAEYFQREIGRAERTAAPLSIALIDVDNFKQL
HHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHCHHHHCCCCEEEEEEECHHHHHH
NDRYGHLAGDDALKYLVDTIKHSLRPADIVARFGGEEFVILMPDTPETEAVQTVQRLQRE
HHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHH
LTKTFFLANNDKLLITFSAGVARWHLGEQESDVLERADHAMYQAKINGKNRVCSAEDMPG
HHHHHEEECCCEEEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHEECCCCCEECCCCCCC
>Mature Secondary Structure 
PALDGADEAASDASGSWQAWQRTLAFALKHGLEPRLSDYPDLVDGLESLLQELEGIADD
CCCCCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
PSLNVFMPKLRAFMIKLELQSQQEGRLATGLANLLKLMLDNVAELNRSDEYLLGQVGSLQ
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCHHH
EILSHEPLSMQQIYQLETSLKEVIRKQGMLKSSLDEAASSLRALLDRFVSRLALMTDSTD
HHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EFHGKIQAHSEKLKQMPNVDELGGIIGSLMEDTSIMQLDLTRSRDELVSAKEQVEAAEHR
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHH
IRELETALEAASAKVKEDQLTGAYNRRGLAEYFQREIGRAERTAAPLSIALIDVDNFKQL
HHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHCHHHHCCCCEEEEEEECHHHHHH
NDRYGHLAGDDALKYLVDTIKHSLRPADIVARFGGEEFVILMPDTPETEAVQTVQRLQRE
HHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHH
LTKTFFLANNDKLLITFSAGVARWHLGEQESDVLERADHAMYQAKINGKNRVCSAEDMPG
HHHHHEEECCCEEEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHEECCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]