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The map label for this gene is pyrG

Identifier: 34498912

GI number: 34498912

Start: 3752408

End: 3754048

Strand: Direct

Name: pyrG

Synonym: CV_3457

Alternate gene names: 34498912

Gene position: 3752408-3754048 (Clockwise)

Preceding gene: 34498909

Following gene: 34498914

Centisome position: 78.98

GC content: 63.86

Gene sequence:

>1641_bases
ATGACCAAGTACATCTTCGTAACCGGTGGCGTGGTGTCCTCCCTGGGCAAGGGCATTGCCGCTGCGTCCATCGCCGCGAT
CCTCGAATCCCGCGGGCTGAAAGTCACCATGCTGAAGCTCGATCCCTATATCAACGTCGACCCGGGCACGATGAGCCCCT
TCCAGCACGGCGAGGTGTTCGTCACCGAAGACGGCGCGGAAACCGACCTTGACCTCGGCCACTACGAGCGCTTCATCCAC
GCCAAGATGAAGAAGAGCAACAACTTCACCACCGGCCAGGTGTACGAATCGGTCATCACCAAGGAGCGCCGCGGCGACTA
CCTGGGCGGCACCGTGCAGGTCATCCCGCACATCACCGACGAAATCAAGCTGAAAATGGGCGAAGGCGCGGCTGACGCCG
ACGTCGCCATCGTCGAGATCGGCGGCACCGTCGGCGACATCGAATCGCTGCCCTTCCTGGAAGCCATCCGCCAGATGCGC
TCCAACCACGGCCGCAAGAACACGCTCTACGTGCACCTCTCCTACGTGCCCTACATCGCCACCGCCGGCGAAATCAAGAC
CAAGCCGACCCAGCACTCGGTGAAGGAGCTGCGCGAGATCGGCATCCAGCCGGACATCCTGCTGTGCCGGATGGACCGCG
AGCTGCCGGAGGACGAAAAGCGCAAGATCGCGCTGTTCTGCAACGTGGAAGAGAACGCGGTGATCGGCTGTTACGACTCC
GACTCCATCTACAAGGTGCCGGGCATGCTGCACGCCCAGGGCATCGACGACATCATCGCCGAACAGCTGCAACTGGACCT
GCCCCAGGCCGACCTGTCCGCCTGGGACCGCATCATCGACGCGATCGAAAAGCCGGACCACGCGATCAAGATCGCGATGG
TGGGCAAGTACGTCGATCTGACCGAATCGTACAAATCGCTGACCGAAGCGCTGAAGCACGCCGGCATCCACACCCGCACC
AAGGTCGACATCATTTACGTCGACTCCGAGGAAATCGAGCGCGACGGCGCCGAGTCTCTGCGCGACATGGACGCCATCCT
GGTGCCGGGCGGCTTCGGCAAGCGCGGCGTGGAAGGCAAGATCAAGGCGGTGAAATTCGCCCGCGAGAACGACATCCCCT
ACCTGGGCATCTGCCTGGGCATGCAGATCGCGCTGATCGAATACGCCCGCGACGTGGCCGGCATGGCCGGCGCCAACTCC
ACCGAGTTCGACCTGGACACCAACTTCCCGGTGGTGGCGCTGATCGACGAGTGGGTGAACCACGACGGCAAGATCGAGAA
GCGCGACGAGAACTCCAATATGGGCGGCACCATGCGCCTGGGCGGCCAGCAGTGCGACCTGGTTCCCGGCTCGCTGGCCG
CGCGCGTCTACGGCAGCACAGAGATCACCGAGCGCCACCGCCACCGCTACGAGGTCAACAACTACTACATCCAGCGCCTG
GAACAGGCCGGCCTGACCATCAGCGGCCGCTCCGCCGGCCACGAGAAGCTGGTCGAGACCATCGAGCTGACGGGCCACCG
CTGGTTCTTCGCCTGCCAGTTCCACCCGGAATTCACCTCCACCCCGCGTGACGGCCATCCGCTGTTCATCGCCTACGTGA
AGGCCGCGCTGGCGCACCAGGCCGACAAGCAGGCCAGCTGA

Upstream 100 bases:

>100_bases
CATTCTGCGCCGCAGACGGGGCCGCAACAAGATGCAGCAAGTGATTCAACTTCGTCCGATTTCAGGGTAAAATCCCGGAT
TCCCACAGAGCCGTAATCTC

Downstream 100 bases:

>100_bases
TTGGCAATTGCTGACCACGCTCAATGCGCCCCATGAGCGTGCGCAGCACAATCCCAAGCTGAGCACTTGCTGTAACACAC
AGCTGGTAATGTCCGCCCGC

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MTKYIFVTGGVVSSLGKGIAAASIAAILESRGLKVTMLKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFIH
AKMKKSNNFTTGQVYESVITKERRGDYLGGTVQVIPHITDEIKLKMGEGAADADVAIVEIGGTVGDIESLPFLEAIRQMR
SNHGRKNTLYVHLSYVPYIATAGEIKTKPTQHSVKELREIGIQPDILLCRMDRELPEDEKRKIALFCNVEENAVIGCYDS
DSIYKVPGMLHAQGIDDIIAEQLQLDLPQADLSAWDRIIDAIEKPDHAIKIAMVGKYVDLTESYKSLTEALKHAGIHTRT
KVDIIYVDSEEIERDGAESLRDMDAILVPGGFGKRGVEGKIKAVKFARENDIPYLGICLGMQIALIEYARDVAGMAGANS
TEFDLDTNFPVVALIDEWVNHDGKIEKRDENSNMGGTMRLGGQQCDLVPGSLAARVYGSTEITERHRHRYEVNNYYIQRL
EQAGLTISGRSAGHEKLVETIELTGHRWFFACQFHPEFTSTPRDGHPLFIAYVKAALAHQADKQAS

Sequences:

>Translated_546_residues
MTKYIFVTGGVVSSLGKGIAAASIAAILESRGLKVTMLKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFIH
AKMKKSNNFTTGQVYESVITKERRGDYLGGTVQVIPHITDEIKLKMGEGAADADVAIVEIGGTVGDIESLPFLEAIRQMR
SNHGRKNTLYVHLSYVPYIATAGEIKTKPTQHSVKELREIGIQPDILLCRMDRELPEDEKRKIALFCNVEENAVIGCYDS
DSIYKVPGMLHAQGIDDIIAEQLQLDLPQADLSAWDRIIDAIEKPDHAIKIAMVGKYVDLTESYKSLTEALKHAGIHTRT
KVDIIYVDSEEIERDGAESLRDMDAILVPGGFGKRGVEGKIKAVKFARENDIPYLGICLGMQIALIEYARDVAGMAGANS
TEFDLDTNFPVVALIDEWVNHDGKIEKRDENSNMGGTMRLGGQQCDLVPGSLAARVYGSTEITERHRHRYEVNNYYIQRL
EQAGLTISGRSAGHEKLVETIELTGHRWFFACQFHPEFTSTPRDGHPLFIAYVKAALAHQADKQAS
>Mature_545_residues
TKYIFVTGGVVSSLGKGIAAASIAAILESRGLKVTMLKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFIHA
KMKKSNNFTTGQVYESVITKERRGDYLGGTVQVIPHITDEIKLKMGEGAADADVAIVEIGGTVGDIESLPFLEAIRQMRS
NHGRKNTLYVHLSYVPYIATAGEIKTKPTQHSVKELREIGIQPDILLCRMDRELPEDEKRKIALFCNVEENAVIGCYDSD
SIYKVPGMLHAQGIDDIIAEQLQLDLPQADLSAWDRIIDAIEKPDHAIKIAMVGKYVDLTESYKSLTEALKHAGIHTRTK
VDIIYVDSEEIERDGAESLRDMDAILVPGGFGKRGVEGKIKAVKFARENDIPYLGICLGMQIALIEYARDVAGMAGANST
EFDLDTNFPVVALIDEWVNHDGKIEKRDENSNMGGTMRLGGQQCDLVPGSLAARVYGSTEITERHRHRYEVNNYYIQRLE
QAGLTISGRSAGHEKLVETIELTGHRWFFACQFHPEFTSTPRDGHPLFIAYVKAALAHQADKQAS

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=568, Percent_Identity=43.8380281690141, Blast_Score=478, Evalue=1e-135,
Organism=Homo sapiens, GI28559085, Length=558, Percent_Identity=43.5483870967742, Blast_Score=464, Evalue=1e-130,
Organism=Homo sapiens, GI28559083, Length=558, Percent_Identity=43.5483870967742, Blast_Score=464, Evalue=1e-130,
Organism=Homo sapiens, GI221316689, Length=558, Percent_Identity=43.5483870967742, Blast_Score=464, Evalue=1e-130,
Organism=Escherichia coli, GI1789142, Length=539, Percent_Identity=65.3061224489796, Blast_Score=721, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25148299, Length=609, Percent_Identity=37.9310344827586, Blast_Score=422, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6322563, Length=570, Percent_Identity=42.280701754386, Blast_Score=454, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6319432, Length=569, Percent_Identity=41.8277680140597, Blast_Score=438, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24664469, Length=559, Percent_Identity=43.8282647584973, Blast_Score=463, Evalue=1e-130,
Organism=Drosophila melanogaster, GI21357815, Length=504, Percent_Identity=42.0634920634921, Blast_Score=390, Evalue=1e-108,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_CHRVO (Q7NSG9)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903127.1
- HSSP:   P0A7E5
- ProteinModelPortal:   Q7NSG9
- SMR:   Q7NSG9
- GeneID:   2547824
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3457
- NMPDR:   fig|243365.1.peg.3457
- HOGENOM:   HBG597806
- OMA:   RVTMQKL
- PhylomeDB:   Q7NSG9
- ProtClustDB:   PRK05380
- BioCyc:   CVIO243365:CV_3457-MONOMER
- BRENDA:   6.3.4.2
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 60466; Mature: 60334

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 378-378 ACT_SITE 515-515 ACT_SITE 517-517

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYIFVTGGVVSSLGKGIAAASIAAILESRGLKVTMLKLDPYINVDPGTMSPFQHGEVF
CCEEEEEECHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTEDGAETDLDLGHYERFIHAKMKKSNNFTTGQVYESVITKERRGDYLGGTVQVIPHITD
EECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
EIKLKMGEGAADADVAIVEIGGTVGDIESLPFLEAIRQMRSNHGRKNTLYVHLSYVPYIA
EEEEEECCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCEEE
TAGEIKTKPTQHSVKELREIGIQPDILLCRMDRELPEDEKRKIALFCNVEENAVIGCYDS
ECCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCEEEEECC
DSIYKVPGMLHAQGIDDIIAEQLQLDLPQADLSAWDRIIDAIEKPDHAIKIAMVGKYVDL
CCEEECCCCHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHH
TESYKSLTEALKHAGIHTRTKVDIIYVDSEEIERDGAESLRDMDAILVPGGFGKRGVEGK
HHHHHHHHHHHHHCCCCCEEEEEEEEECHHHHHCCHHHHHHCCCEEEECCCCCCCCCCCC
IKAVKFARENDIPYLGICLGMQIALIEYARDVAGMAGANSTEFDLDTNFPVVALIDEWVN
EEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEEHHHHC
HDGKIEKRDENSNMGGTMRLGGQQCDLVPGSLAARVYGSTEITERHRHRYEVNNYYIQRL
CCCCEEECCCCCCCCCEEEECCCCCCCCCCCHHEEECCCCHHHHHHHHHHHHHHHHHHHH
EQAGLTISGRSAGHEKLVETIELTGHRWFFACQFHPEFTSTPRDGHPLFIAYVKAALAHQ
HHCCCEEECCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
ADKQAS
HCCCCC
>Mature Secondary Structure 
TKYIFVTGGVVSSLGKGIAAASIAAILESRGLKVTMLKLDPYINVDPGTMSPFQHGEVF
CEEEEEECHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTEDGAETDLDLGHYERFIHAKMKKSNNFTTGQVYESVITKERRGDYLGGTVQVIPHITD
EECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
EIKLKMGEGAADADVAIVEIGGTVGDIESLPFLEAIRQMRSNHGRKNTLYVHLSYVPYIA
EEEEEECCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCEEE
TAGEIKTKPTQHSVKELREIGIQPDILLCRMDRELPEDEKRKIALFCNVEENAVIGCYDS
ECCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCEEEEECC
DSIYKVPGMLHAQGIDDIIAEQLQLDLPQADLSAWDRIIDAIEKPDHAIKIAMVGKYVDL
CCEEECCCCHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHH
TESYKSLTEALKHAGIHTRTKVDIIYVDSEEIERDGAESLRDMDAILVPGGFGKRGVEGK
HHHHHHHHHHHHHCCCCCEEEEEEEEECHHHHHCCHHHHHHCCCEEEECCCCCCCCCCCC
IKAVKFARENDIPYLGICLGMQIALIEYARDVAGMAGANSTEFDLDTNFPVVALIDEWVN
EEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEEHHHHC
HDGKIEKRDENSNMGGTMRLGGQQCDLVPGSLAARVYGSTEITERHRHRYEVNNYYIQRL
CCCCEEECCCCCCCCCEEEECCCCCCCCCCCHHEEECCCCHHHHHHHHHHHHHHHHHHHH
EQAGLTISGRSAGHEKLVETIELTGHRWFFACQFHPEFTSTPRDGHPLFIAYVKAALAHQ
HHCCCEEECCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
ADKQAS
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782