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The map label for this gene is gcvH

Identifier: 34498885

GI number: 34498885

Start: 3726586

End: 3726972

Strand: Reverse

Name: gcvH

Synonym: CV_3430

Alternate gene names: 34498885

Gene position: 3726972-3726586 (Counterclockwise)

Preceding gene: 34498886

Following gene: 34498884

Centisome position: 78.44

GC content: 65.12

Gene sequence:

>387_bases
ATGAGCAACATTCCCGCCGAACTGAAATACGTCGACAGCCACGAATGGCTGCGCCTGGAAGCCGACGGCTCCGTGACCGT
GGGCATCACCGCGCACGCGCAGGAATTGCTGGGCGACATCGTCTTCGTCGAGCTGCCGAAAGTCGGCGCGAGTCTGGCCA
AGGATGAGCAGGCCGGCGTGGTGGAGTCGGTGAAGGCCGCTTCCGACGTCTACTGTCCGATCGCCGGCGAAGTGCTGGCC
GTCAACGAGGAGCTGGAGGGCGAGCCGGAGCTGGCCAACAGCGATCCGTACGGCGACGGCTGGTTCTTCAAGATCAAGCC
GGCCAACGCCGCCGATCTGAACGGCCTGATGGACGCCGCCGCCTACGCCAAGGAAATCGGCGCCTGA

Upstream 100 bases:

>100_bases
TGAAGATGCCCTTCGTCCGCAACGGCAAGAAAGTATTCGAATAAACCGGCCGGCGCGCCGCGCCGCCATCCCGATCATCA
ATACATCTGGAGAAAACGAG

Downstream 100 bases:

>100_bases
TAGCCTGACCGCCCCGCCGTCGCCAGACGCCGGGGCGTTGTTTTTCCCGGCACGGCCTCGCCCAGGCCGCCGCAAAGAAG
GAAGTCCCAAGCATGTCGCT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 128; Mature: 127

Protein sequence:

>128_residues
MSNIPAELKYVDSHEWLRLEADGSVTVGITAHAQELLGDIVFVELPKVGASLAKDEQAGVVESVKAASDVYCPIAGEVLA
VNEELEGEPELANSDPYGDGWFFKIKPANAADLNGLMDAAAYAKEIGA

Sequences:

>Translated_128_residues
MSNIPAELKYVDSHEWLRLEADGSVTVGITAHAQELLGDIVFVELPKVGASLAKDEQAGVVESVKAASDVYCPIAGEVLA
VNEELEGEPELANSDPYGDGWFFKIKPANAADLNGLMDAAAYAKEIGA
>Mature_127_residues
SNIPAELKYVDSHEWLRLEADGSVTVGITAHAQELLGDIVFVELPKVGASLAKDEQAGVVESVKAASDVYCPIAGEVLAV
NEELEGEPELANSDPYGDGWFFKIKPANAADLNGLMDAAAYAKEIGA

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=118, Percent_Identity=46.6101694915254, Blast_Score=109, Evalue=7e-25,
Organism=Homo sapiens, GI89057342, Length=118, Percent_Identity=45.7627118644068, Blast_Score=106, Evalue=7e-24,
Organism=Escherichia coli, GI1789271, Length=122, Percent_Identity=61.4754098360656, Blast_Score=163, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17507493, Length=115, Percent_Identity=47.8260869565217, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17551294, Length=116, Percent_Identity=43.9655172413793, Blast_Score=102, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6319272, Length=125, Percent_Identity=40.8, Blast_Score=106, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17865652, Length=121, Percent_Identity=42.9752066115703, Blast_Score=112, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_CHRVO (Q7NSJ4)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903100.1
- HSSP:   P16048
- ProteinModelPortal:   Q7NSJ4
- SMR:   Q7NSJ4
- GeneID:   2548313
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3430
- NMPDR:   fig|243365.1.peg.3430
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- PhylomeDB:   Q7NSJ4
- ProtClustDB:   PRK01202
- BioCyc:   CVIO243365:CV_3430-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13518; Mature: 13387

Theoretical pI: Translated: 3.93; Mature: 3.93

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIPAELKYVDSHEWLRLEADGSVTVGITAHAQELLGDIVFVELPKVGASLAKDEQAGV
CCCCCCCEEEECCCCEEEEECCCCEEEEEEHHHHHHHHCEEEEECCCCCCHHCCCCCCCH
VESVKAASDVYCPIAGEVLAVNEELEGEPELANSDPYGDGWFFKIKPANAADLNGLMDAA
HHHHHHCCCEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHH
AYAKEIGA
HHHHHHCC
>Mature Secondary Structure 
SNIPAELKYVDSHEWLRLEADGSVTVGITAHAQELLGDIVFVELPKVGASLAKDEQAGV
CCCCCCEEEECCCCEEEEECCCCEEEEEEHHHHHHHHCEEEEECCCCCCHHCCCCCCCH
VESVKAASDVYCPIAGEVLAVNEELEGEPELANSDPYGDGWFFKIKPANAADLNGLMDAA
HHHHHHCCCEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHH
AYAKEIGA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782