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The map label for this gene is gcvT

Identifier: 34498886

GI number: 34498886

Start: 3727029

End: 3728117

Strand: Reverse

Name: gcvT

Synonym: CV_3431

Alternate gene names: 34498886

Gene position: 3728117-3727029 (Counterclockwise)

Preceding gene: 34498887

Following gene: 34498885

Centisome position: 78.47

GC content: 65.01

Gene sequence:

>1089_bases
ATGACGGCCCCGAAACGTACCCCGCTGTTTGATGCCCATATCGCCGCCGGCGCCAAGATGGTCGATTTCGCCGGTTGGGA
AATGCCCATCCACTATGGTTCCCAGCTGAAAGAGCACGAAATCGTCCGCAGCGACGCGGGCATGTTCGACGTCTCGCACA
TGACGGTGATCGACATCACCGGCGCCGACGCCAAGGCCTGGCTGCAAAAACTGATCGCTAACGACGTGGCCAAGCTCGGC
TTCGAGGGCAAGGCGCTGTATTCCGGCATGCTGACCCCGGAAGGCACCGTCGTCGACGACCTGATCGTCTACCTCACTGC
CTACGGCTACCGCATGGTGGTCAACGCCGGCACCACCGAGAAAGACTTGGCCTGGATGGAAAGCCAGAAGGCCGGCTTCG
ACGTGGCGCTGAAGGTGCGCCGCGACCTGGCGATGCTGGCGGTGCAGGGCCCCAAGGCGATTCAAAAAGTCTGTTCCATC
AAGCCGGAGCTGGCCGAGGCCATCCGCGCGCTGAAAGTGTTCCAGGGCCTGCCGCAGGGCGAGTGGTTCTACGCCCGCAC
CGGCTATACCGGCGAAGACGGCCTGGAAATCATGGTCCCGGCCGATCAGGCCATTTCCTTCTTCCATCAATTGCAAGCCG
CCGGCGTGTCGCCGATCGGCCTGGGCGCGCGCGACACGCTGCGCTTGGAAGCCGGCATGAACCTGTACGGCCACGACATG
GACGAGACCGTGTCGCCGCTGGAAGCCGGCATGGGCTGGACCATCGCCTGGGAGCCGGCCGAGCGCAAGTTCAACGGCCG
CGAGGCGCTGGAAGCGCAAAAGGCCGCCGGCGTGAAGATGAAGCAGGTGGGCCTGGTGCTGGAAGGCCGCGGCGTGCTGC
GCGAAGGCCTGAAGGTGGTGGTGGAGAGCGTCGGCGAAGGCGTGATCACCAGCGGCACCTTCTCGCCTACGCTGAAGCAT
TCCATCGCCATCGCCCGCGTGCCGGCCGCCACCGGCGCCGCCGCCCAGGTGGACCTGCGCGGCACGCTGACCGACGTCCG
CGTGGTGAAGATGCCCTTCGTCCGCAACGGCAAGAAAGTATTCGAATAA

Upstream 100 bases:

>100_bases
CGAAGGGGCTAACGGCTTATCCGGCCGGAAAATCTCAGGTGCAGGGACAGAGGGGTGTGGTTGATGAGGCTATCCACACT
ATTCTCGAAGGAACCGCGCC

Downstream 100 bases:

>100_bases
ACCGGCCGGCGCGCCGCGCCGCCATCCCGATCATCAATACATCTGGAGAAAACGAGATGAGCAACATTCCCGCCGAACTG
AAATACGTCGACAGCCACGA

Product: glycine cleavage system aminomethyltransferase T

Products: NA

Alternate protein names: Glycine cleavage system T protein

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MTAPKRTPLFDAHIAAGAKMVDFAGWEMPIHYGSQLKEHEIVRSDAGMFDVSHMTVIDITGADAKAWLQKLIANDVAKLG
FEGKALYSGMLTPEGTVVDDLIVYLTAYGYRMVVNAGTTEKDLAWMESQKAGFDVALKVRRDLAMLAVQGPKAIQKVCSI
KPELAEAIRALKVFQGLPQGEWFYARTGYTGEDGLEIMVPADQAISFFHQLQAAGVSPIGLGARDTLRLEAGMNLYGHDM
DETVSPLEAGMGWTIAWEPAERKFNGREALEAQKAAGVKMKQVGLVLEGRGVLREGLKVVVESVGEGVITSGTFSPTLKH
SIAIARVPAATGAAAQVDLRGTLTDVRVVKMPFVRNGKKVFE

Sequences:

>Translated_362_residues
MTAPKRTPLFDAHIAAGAKMVDFAGWEMPIHYGSQLKEHEIVRSDAGMFDVSHMTVIDITGADAKAWLQKLIANDVAKLG
FEGKALYSGMLTPEGTVVDDLIVYLTAYGYRMVVNAGTTEKDLAWMESQKAGFDVALKVRRDLAMLAVQGPKAIQKVCSI
KPELAEAIRALKVFQGLPQGEWFYARTGYTGEDGLEIMVPADQAISFFHQLQAAGVSPIGLGARDTLRLEAGMNLYGHDM
DETVSPLEAGMGWTIAWEPAERKFNGREALEAQKAAGVKMKQVGLVLEGRGVLREGLKVVVESVGEGVITSGTFSPTLKH
SIAIARVPAATGAAAQVDLRGTLTDVRVVKMPFVRNGKKVFE
>Mature_361_residues
TAPKRTPLFDAHIAAGAKMVDFAGWEMPIHYGSQLKEHEIVRSDAGMFDVSHMTVIDITGADAKAWLQKLIANDVAKLGF
EGKALYSGMLTPEGTVVDDLIVYLTAYGYRMVVNAGTTEKDLAWMESQKAGFDVALKVRRDLAMLAVQGPKAIQKVCSIK
PELAEAIRALKVFQGLPQGEWFYARTGYTGEDGLEIMVPADQAISFFHQLQAAGVSPIGLGARDTLRLEAGMNLYGHDMD
ETVSPLEAGMGWTIAWEPAERKFNGREALEAQKAAGVKMKQVGLVLEGRGVLREGLKVVVESVGEGVITSGTFSPTLKHS
IAIARVPAATGAAAQVDLRGTLTDVRVVKMPFVRNGKKVFE

Specific function: The glycine cleavage system catalyzes the degradation of glycine

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family

Homologues:

Organism=Homo sapiens, GI44662838, Length=369, Percent_Identity=39.0243902439024, Blast_Score=202, Evalue=6e-52,
Organism=Homo sapiens, GI257796258, Length=339, Percent_Identity=38.6430678466077, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI257796254, Length=362, Percent_Identity=34.2541436464088, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI257796256, Length=313, Percent_Identity=37.0607028753994, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI24797151, Length=353, Percent_Identity=30.028328611898, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI197927446, Length=381, Percent_Identity=25.1968503937008, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI21361378, Length=381, Percent_Identity=25.1968503937008, Blast_Score=82, Evalue=8e-16,
Organism=Escherichia coli, GI1789272, Length=362, Percent_Identity=55.524861878453, Blast_Score=386, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17560118, Length=377, Percent_Identity=36.3395225464191, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI71994052, Length=329, Percent_Identity=24.6200607902736, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71994045, Length=329, Percent_Identity=24.6200607902736, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320222, Length=378, Percent_Identity=34.6560846560847, Blast_Score=182, Evalue=6e-47,
Organism=Drosophila melanogaster, GI20129441, Length=375, Percent_Identity=33.8666666666667, Blast_Score=174, Evalue=6e-44,
Organism=Drosophila melanogaster, GI20130091, Length=433, Percent_Identity=21.9399538106236, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): GCST_CHRVO (Q7NSJ3)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903101.1
- ProteinModelPortal:   Q7NSJ3
- SMR:   Q7NSJ3
- GeneID:   2548314
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3431
- NMPDR:   fig|243365.1.peg.3431
- HOGENOM:   HBG299834
- OMA:   KALYGGM
- PhylomeDB:   Q7NSJ3
- ProtClustDB:   PRK00389
- BioCyc:   CVIO243365:CV_3431-MONOMER
- BRENDA:   2.1.2.10
- GO:   GO:0005737
- HAMAP:   MF_00259
- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903
- PIRSF:   PIRSF006487
- TIGRFAMs:   TIGR00528

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C

EC number: =2.1.2.10

Molecular weight: Translated: 39136; Mature: 39005

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPKRTPLFDAHIAAGAKMVDFAGWEMPIHYGSQLKEHEIVRSDAGMFDVSHMTVIDIT
CCCCCCCCCCHHHHHCCCEEEECCCCCCCCHHCCCHHHHHHHHCCCCEEEECCEEEEEEE
GADAKAWLQKLIANDVAKLGFEGKALYSGMLTPEGTVVDDLIVYLTAYGYRMVVNAGTTE
CCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCH
KDLAWMESQKAGFDVALKVRRDLAMLAVQGPKAIQKVCSIKPELAEAIRALKVFQGLPQG
HHHHHHHHCCCCCCEEEHHHHCCCEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
EWFYARTGYTGEDGLEIMVPADQAISFFHQLQAAGVSPIGLGARDTLRLEAGMNLYGHDM
CEEEEECCCCCCCCCEEEECHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCH
DETVSPLEAGMGWTIAWEPAERKFNGREALEAQKAAGVKMKQVGLVLEGRGVLREGLKVV
HHHHHHHHHCCCEEEEECCHHCCCCCHHHHHHHHHCCCEEHHCCEEEECCHHHHHHHHHH
VESVGEGVITSGTFSPTLKHSIAIARVPAATGAAAQVDLRGTLTDVRVVKMPFVRNGKKV
HHHHCCCEEECCCCCCHHHHCEEEEECCCCCCCEEEEEECCCCCCEEEEEECCCCCCHHH
FE
CC
>Mature Secondary Structure 
TAPKRTPLFDAHIAAGAKMVDFAGWEMPIHYGSQLKEHEIVRSDAGMFDVSHMTVIDIT
CCCCCCCCCHHHHHCCCEEEECCCCCCCCHHCCCHHHHHHHHCCCCEEEECCEEEEEEE
GADAKAWLQKLIANDVAKLGFEGKALYSGMLTPEGTVVDDLIVYLTAYGYRMVVNAGTTE
CCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCH
KDLAWMESQKAGFDVALKVRRDLAMLAVQGPKAIQKVCSIKPELAEAIRALKVFQGLPQG
HHHHHHHHCCCCCCEEEHHHHCCCEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
EWFYARTGYTGEDGLEIMVPADQAISFFHQLQAAGVSPIGLGARDTLRLEAGMNLYGHDM
CEEEEECCCCCCCCCEEEECHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCH
DETVSPLEAGMGWTIAWEPAERKFNGREALEAQKAAGVKMKQVGLVLEGRGVLREGLKVV
HHHHHHHHHCCCEEEEECCHHCCCCCHHHHHHHHHCCCEEHHCCEEEECCHHHHHHHHHH
VESVGEGVITSGTFSPTLKHSIAIARVPAATGAAAQVDLRGTLTDVRVVKMPFVRNGKKV
HHHHCCCEEECCCCCCHHHHCEEEEECCCCCCCEEEEEECCCCCCEEEEEECCCCCCHHH
FE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782