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The map label for this gene is miaA

Identifier: 34498844

GI number: 34498844

Start: 3682719

End: 3683696

Strand: Direct

Name: miaA

Synonym: CV_3389

Alternate gene names: 34498844

Gene position: 3682719-3683696 (Clockwise)

Preceding gene: 34498838

Following gene: 34498847

Centisome position: 77.51

GC content: 69.63

Gene sequence:

>978_bases
ATGAGCGACACCCCACAAGCGATACTGCTGATGGGCCCCACCGCCTCCGGCAAGACCGGGCTGGCGCTGGAGCTGGCCCG
TCGTTTCCCCGTCGAAATCGTCAGCGTCGATTCGGCGCTGGTCTACCGCGACATGGATATCGGCACCGCCAAGCCCACGG
CCGAGGAGATGGCCGCCTGTCCCCATCATCTGATAGACGTCATCAGCCCGCTGCAAAGCTACTCCGCCGCGCAGTTCCAT
GCCGACGCCAACCGGCTGATCGCCGACATCCAGGCGCGCGGCAAGCTGCCGCTGCTGGTGGGCGGCACCATGCTTTATTA
CAAGGCGCTGCTGGAAGGCCTGTCCGACCTGCCTCAGGCCGACGCCGCGCTACGCGCCGAGCTAGACGCCGACGCCGCGC
TGCTGGGCTGGCCGGCAATGCACGCGCGCCTGGCCGAACTCGACCCGGCGACCGCCTCCCGCCTCAATCCCAACGACTCG
CAGCGCATCCACCGCGCGCTGGAAGTGTGCCTGCTCAGCGGCAAGCCGATGTCCGAGCTGATCGCCCAGGGCAAGGAAGC
CGCCGCAGGCTTCCGTTTCCTGCCGCTGGCGCTGGTGCCGCGCGAGCGCGGCTGGCTGCACGCGCGCATCGCCGAGCGCT
TCCGCATCATGCTGGAGCAGGGCTTTCTGGACGAAGTCAGCCGATTGCGGGCGAAATATCCGGAACTGACGCTGAACCTG
CCGTCGATGCGCTGCGTCGGCTACCGCCAGGCCTGGGAGCACCAGGACGGCCTGTACGGCCACGACGAATTCGTCGAGCG
CGGCGTCGCCGCCACCCGCCAGCTGGCCAAGCGCCAGCTGACCTGGACCCGCAGCCTGGACGCGATCCCGGTCGACGCGC
AGCGCGACGGCCTGGCCGGGCTGCTGGGCGACGCGGTGGACGATTTCCTCGCCGGCCGGCCGCCGGCCGAGTCGCTGCGC
TACGCCGGCGATTTCTGA

Upstream 100 bases:

>100_bases
GATACTCACATCGTAAACAGCTGAGCGGCAAGGTCAAGCCTTTCGCGCATGCGCGCCCCGGCTGTGCGACAATCGCCCCC
TGACCGCAGCGAGCAACACC

Downstream 100 bases:

>100_bases
GAGACAAACGGCGGGCCGGCCCGCCGTTTCCGCATCGCCCCGCAAGCCGCTCAGGGGCTGACGCTCATCCGCAGCGTGTG
CGTCCAGTTGTGGTCGCTGG

Product: tRNA isopentenyltransferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSDTPQAILLMGPTASGKTGLALELARRFPVEIVSVDSALVYRDMDIGTAKPTAEEMAACPHHLIDVISPLQSYSAAQFH
ADANRLIADIQARGKLPLLVGGTMLYYKALLEGLSDLPQADAALRAELDADAALLGWPAMHARLAELDPATASRLNPNDS
QRIHRALEVCLLSGKPMSELIAQGKEAAAGFRFLPLALVPRERGWLHARIAERFRIMLEQGFLDEVSRLRAKYPELTLNL
PSMRCVGYRQAWEHQDGLYGHDEFVERGVAATRQLAKRQLTWTRSLDAIPVDAQRDGLAGLLGDAVDDFLAGRPPAESLR
YAGDF

Sequences:

>Translated_325_residues
MSDTPQAILLMGPTASGKTGLALELARRFPVEIVSVDSALVYRDMDIGTAKPTAEEMAACPHHLIDVISPLQSYSAAQFH
ADANRLIADIQARGKLPLLVGGTMLYYKALLEGLSDLPQADAALRAELDADAALLGWPAMHARLAELDPATASRLNPNDS
QRIHRALEVCLLSGKPMSELIAQGKEAAAGFRFLPLALVPRERGWLHARIAERFRIMLEQGFLDEVSRLRAKYPELTLNL
PSMRCVGYRQAWEHQDGLYGHDEFVERGVAATRQLAKRQLTWTRSLDAIPVDAQRDGLAGLLGDAVDDFLAGRPPAESLR
YAGDF
>Mature_324_residues
SDTPQAILLMGPTASGKTGLALELARRFPVEIVSVDSALVYRDMDIGTAKPTAEEMAACPHHLIDVISPLQSYSAAQFHA
DANRLIADIQARGKLPLLVGGTMLYYKALLEGLSDLPQADAALRAELDADAALLGWPAMHARLAELDPATASRLNPNDSQ
RIHRALEVCLLSGKPMSELIAQGKEAAAGFRFLPLALVPRERGWLHARIAERFRIMLEQGFLDEVSRLRAKYPELTLNLP
SMRCVGYRQAWEHQDGLYGHDEFVERGVAATRQLAKRQLTWTRSLDAIPVDAQRDGLAGLLGDAVDDFLAGRPPAESLRY
AGDF

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=315, Percent_Identity=29.2063492063492, Blast_Score=129, Evalue=3e-30,
Organism=Escherichia coli, GI1790613, Length=285, Percent_Identity=55.0877192982456, Blast_Score=303, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI25144712, Length=267, Percent_Identity=27.3408239700375, Blast_Score=95, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6324848, Length=335, Percent_Identity=29.8507462686567, Blast_Score=139, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24649830, Length=259, Percent_Identity=31.6602316602317, Blast_Score=129, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_CHRVO (Q7MBE2)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903059.1
- ProteinModelPortal:   Q7MBE2
- SMR:   Q7MBE2
- GeneID:   2548781
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3389
- NMPDR:   fig|243365.1.peg.3389
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   Q7MBE2
- BioCyc:   CVIO243365:CV_3389-MONOMER
- BRENDA:   2.5.1.8
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35584; Mature: 35453

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTPQAILLMGPTASGKTGLALELARRFPVEIVSVDSALVYRDMDIGTAKPTAEEMAAC
CCCCCCEEEEECCCCCCCCCHHHHHHHHCCEEEEECCCCCEEECCCCCCCCCCHHHHHHC
PHHLIDVISPLQSYSAAQFHADANRLIADIQARGKLPLLVGGTMLYYKALLEGLSDLPQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHCCCCH
DAALRAELDADAALLGWPAMHARLAELDPATASRLNPNDSQRIHRALEVCLLSGKPMSEL
HHHHHHHCCCCHHHHCCHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHH
IAQGKEAAAGFRFLPLALVPRERGWLHARIAERFRIMLEQGFLDEVSRLRAKYPELTLNL
HHCCHHHHCCCEEEHEEECCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCEEEEC
PSMRCVGYRQAWEHQDGLYGHDEFVERGVAATRQLAKRQLTWTRSLDAIPVDAQRDGLAG
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
LLGDAVDDFLAGRPPAESLRYAGDF
HHHHHHHHHHCCCCCHHHHCCCCCC
>Mature Secondary Structure 
SDTPQAILLMGPTASGKTGLALELARRFPVEIVSVDSALVYRDMDIGTAKPTAEEMAAC
CCCCCEEEEECCCCCCCCCHHHHHHHHCCEEEEECCCCCEEECCCCCCCCCCHHHHHHC
PHHLIDVISPLQSYSAAQFHADANRLIADIQARGKLPLLVGGTMLYYKALLEGLSDLPQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHCCCCH
DAALRAELDADAALLGWPAMHARLAELDPATASRLNPNDSQRIHRALEVCLLSGKPMSEL
HHHHHHHCCCCHHHHCCHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHH
IAQGKEAAAGFRFLPLALVPRERGWLHARIAERFRIMLEQGFLDEVSRLRAKYPELTLNL
HHCCHHHHCCCEEEHEEECCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCEEEEC
PSMRCVGYRQAWEHQDGLYGHDEFVERGVAATRQLAKRQLTWTRSLDAIPVDAQRDGLAG
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
LLGDAVDDFLAGRPPAESLRYAGDF
HHHHHHHHHHCCCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782