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The map label for this gene is greB [H]

Identifier: 34498839

GI number: 34498839

Start: 3678783

End: 3679337

Strand: Reverse

Name: greB [H]

Synonym: CV_3384

Alternate gene names: 34498839

Gene position: 3679337-3678783 (Counterclockwise)

Preceding gene: 34498840

Following gene: 34498829

Centisome position: 77.44

GC content: 63.96

Gene sequence:

>555_bases
ATGAGCAAGGCGTTTACCAAAGAAGACGAGCAGCAGGACGATGATCTGCCGGCCGAGCGCCGCCTGCCGGGCTCGACCAA
GAACTACATCACGCCCATGGGCTGGCAGCGGTTGAAGGATGAGCTGTACCACCTGGTCAACCGCGAGCGCCCCGAGATCG
TGCAGGTGGTGAACTGGGCGGCCAGCAACGGCGACCGTTCGGAAAACGGCGACTATCTGTACGGCAAGCGCCGGCTGCGC
GAGATCGACCGCCGCATCCGCTTCCTGACCAAACGATTGGAAATCGCCGAAGTGGTGGATCCGGAAGCCCGCGAGGCGAC
CGACCAGGTGTTCTTCTCCGCCACCGTGCTGATCCAGCGCGGCGACGGCAGCGAGCAAAGGCTGTCCATCGTCGGCGTCG
ACGAGATCGACTTGCAGAGGGGCCGCATCAGCTGGATATCCCCGATCGCGCGCGCGCTGCTGAAGTCGCGCGAGGGCGAC
ACCGTGCTGTTTCGGGGCCCGGATGGAGACGAGGACATCGAAGTGCTTGAAGTCAGCTACGTTCGGCTGGACTGA

Upstream 100 bases:

>100_bases
AAGTTGCGGCCCAAGTCGCTGCGCCCGCATTGCCTGGCGCGGCTGGGAGCCGTGGACGGGACTCGGGCATAATGGCGAAA
TCCTCATCAGGAATACCGAC

Downstream 100 bases:

>100_bases
GGCGGAAATGATGAAAACGGCCCGGAATGCTCCGGGCCGTTTTTGTTTGGGCGGCGGGAGGGATCAGGGCTGCATGTGCT
CGGACAGCTTGCCGTTGATC

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MSKAFTKEDEQQDDDLPAERRLPGSTKNYITPMGWQRLKDELYHLVNRERPEIVQVVNWAASNGDRSENGDYLYGKRRLR
EIDRRIRFLTKRLEIAEVVDPEAREATDQVFFSATVLIQRGDGSEQRLSIVGVDEIDLQRGRISWISPIARALLKSREGD
TVLFRGPDGDEDIEVLEVSYVRLD

Sequences:

>Translated_184_residues
MSKAFTKEDEQQDDDLPAERRLPGSTKNYITPMGWQRLKDELYHLVNRERPEIVQVVNWAASNGDRSENGDYLYGKRRLR
EIDRRIRFLTKRLEIAEVVDPEAREATDQVFFSATVLIQRGDGSEQRLSIVGVDEIDLQRGRISWISPIARALLKSREGD
TVLFRGPDGDEDIEVLEVSYVRLD
>Mature_183_residues
SKAFTKEDEQQDDDLPAERRLPGSTKNYITPMGWQRLKDELYHLVNRERPEIVQVVNWAASNGDRSENGDYLYGKRRLRE
IDRRIRFLTKRLEIAEVVDPEAREATDQVFFSATVLIQRGDGSEQRLSIVGVDEIDLQRGRISWISPIARALLKSREGDT
VLFRGPDGDEDIEVLEVSYVRLD

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=153, Percent_Identity=48.3660130718954, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI87082225, Length=153, Percent_Identity=34.640522875817, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 21263; Mature: 21132

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAFTKEDEQQDDDLPAERRLPGSTKNYITPMGWQRLKDELYHLVNRERPEIVQVVNWA
CCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
ASNGDRSENGDYLYGKRRLREIDRRIRFLTKRLEIAEVVDPEAREATDQVFFSATVLIQR
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEEEEEEC
GDGSEQRLSIVGVDEIDLQRGRISWISPIARALLKSREGDTVLFRGPDGDEDIEVLEVSY
CCCCCCEEEEECCCHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEE
VRLD
EECC
>Mature Secondary Structure 
SKAFTKEDEQQDDDLPAERRLPGSTKNYITPMGWQRLKDELYHLVNRERPEIVQVVNWA
CCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
ASNGDRSENGDYLYGKRRLREIDRRIRFLTKRLEIAEVVDPEAREATDQVFFSATVLIQR
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEEEEEEC
GDGSEQRLSIVGVDEIDLQRGRISWISPIARALLKSREGDTVLFRGPDGDEDIEVLEVSY
CCCCCCEEEEECCCHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEE
VRLD
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]