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The map label for this gene is alkB [H]

Identifier: 34498651

GI number: 34498651

Start: 3480294

End: 3480941

Strand: Reverse

Name: alkB [H]

Synonym: CV_3196

Alternate gene names: 34498651

Gene position: 3480941-3480294 (Counterclockwise)

Preceding gene: 34498652

Following gene: 34498650

Centisome position: 73.27

GC content: 72.22

Gene sequence:

>648_bases
GTGAGCGACAGCCTGTTCGCGCCGGACGACGGCCCGTGGACCGAGCGGCTGTCGGCCGGCGCCGCCTTGCTGCACGGCTG
GGCCTGCGCCGGCGACGCGGAGCTGGTCGCCGCGGTGGAGGCGGTCGTAGCGCAGTCTCCGCTGCGCGGCATGGTGACGC
CCGGCGGGCAGGCCATGTCGGTGACCACCGCCAGCTGCGGCGACTGGGGCTGGGTGTCCGATCGCCGCGGCTACCGCTAT
TCCGCCGTCGATCCGGATAAGGGACGGCCCTGGCCGGCGATGCCTGGGCCGCTGCGCGAACTGGCGCGCGGCGCGGCCGC
CGAGGCGGGTTTTGCCGGCTTCGATCCCGACGCCTGCCTGATCAACTGCTACCTGCCCGGCGCCCGGATGGGCCTGCATC
AGGACAAGGACGAGCGCGATTTCTCCGCGCCCATCGTTTCCGTGTCGCTGGGCCTGCCGGCGATATTCCTGCTGGGCGGC
CTGAGCCGCGCCGACAAAACCGCCCGCATCCTGCTGCAGCATGGCGACGTGCTGGTGTGGGGCGGGCCGGACCGGTTGCG
CTTTCATGGCGTGCTGCCGGTCCAGCCGGGCATTCACCCGCTGCTCGGCGGCCGGCGTCTCAACCTCACTTTCCGCAAGG
CCTTTTGA

Upstream 100 bases:

>100_bases
TGTTCGTCAATATCGAAGCCATCGCCGACGATACGCTGGACCGCGGCGGCCGCCGCATCGGCTCGGTGCTGGTGGGCGGC
ATGATGGTGGCCGGCGAGGA

Downstream 100 bases:

>100_bases
TCCATGTCCGTCATCGATTGCGATATCGCGCTGCCGGCCGGCTTCCGGCCGCAGGACATCCTGGCCTTCCACCGTCGCGA
CGGCGAGCGCCTCGCCGAGC

Product: alkylated DNA repair protein, AlkB protein

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MSDSLFAPDDGPWTERLSAGAALLHGWACAGDAELVAAVEAVVAQSPLRGMVTPGGQAMSVTTASCGDWGWVSDRRGYRY
SAVDPDKGRPWPAMPGPLRELARGAAAEAGFAGFDPDACLINCYLPGARMGLHQDKDERDFSAPIVSVSLGLPAIFLLGG
LSRADKTARILLQHGDVLVWGGPDRLRFHGVLPVQPGIHPLLGGRRLNLTFRKAF

Sequences:

>Translated_215_residues
MSDSLFAPDDGPWTERLSAGAALLHGWACAGDAELVAAVEAVVAQSPLRGMVTPGGQAMSVTTASCGDWGWVSDRRGYRY
SAVDPDKGRPWPAMPGPLRELARGAAAEAGFAGFDPDACLINCYLPGARMGLHQDKDERDFSAPIVSVSLGLPAIFLLGG
LSRADKTARILLQHGDVLVWGGPDRLRFHGVLPVQPGIHPLLGGRRLNLTFRKAF
>Mature_214_residues
SDSLFAPDDGPWTERLSAGAALLHGWACAGDAELVAAVEAVVAQSPLRGMVTPGGQAMSVTTASCGDWGWVSDRRGYRYS
AVDPDKGRPWPAMPGPLRELARGAAAEAGFAGFDPDACLINCYLPGARMGLHQDKDERDFSAPIVSVSLGLPAIFLLGGL
SRADKTARILLQHGDVLVWGGPDRLRFHGVLPVQPGIHPLLGGRRLNLTFRKAF

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=131, Percent_Identity=35.8778625954198, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI1788541, Length=210, Percent_Identity=55.2380952380952, Blast_Score=236, Evalue=6e-64,
Organism=Drosophila melanogaster, GI45555401, Length=98, Percent_Identity=40.8163265306122, Blast_Score=74, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004574 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 22825; Mature: 22694

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSLFAPDDGPWTERLSAGAALLHGWACAGDAELVAAVEAVVAQSPLRGMVTPGGQAMS
CCCCCCCCCCCCHHHHHHHCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCEEE
VTTASCGDWGWVSDRRGYRYSAVDPDKGRPWPAMPGPLRELARGAAAEAGFAGFDPDACL
EEECCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEE
INCYLPGARMGLHQDKDERDFSAPIVSVSLGLPAIFLLGGLSRADKTARILLQHGDVLVW
EEEECCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHCCCCCCCHHHEEEEECCCEEEE
GGPDRLRFHGVLPVQPGIHPLLGGRRLNLTFRKAF
CCCCCEEEEEECCCCCCCCHHCCCEEEEEEEEECC
>Mature Secondary Structure 
SDSLFAPDDGPWTERLSAGAALLHGWACAGDAELVAAVEAVVAQSPLRGMVTPGGQAMS
CCCCCCCCCCCHHHHHHHCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCEEE
VTTASCGDWGWVSDRRGYRYSAVDPDKGRPWPAMPGPLRELARGAAAEAGFAGFDPDACL
EEECCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEE
INCYLPGARMGLHQDKDERDFSAPIVSVSLGLPAIFLLGGLSRADKTARILLQHGDVLVW
EEEECCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHCCCCCCCHHHEEEEECCCEEEE
GGPDRLRFHGVLPVQPGIHPLLGGRRLNLTFRKAF
CCCCCEEEEEECCCCCCCCHHCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536913; 9097040; 9278503; 3887409; 7928996 [H]